Click here to switch to the map view.

The map label for this gene is cbiJ

Identifier: 16765356

GI number: 16765356

Start: 2105211

End: 2106002

Strand: Reverse

Name: cbiJ

Synonym: STM2026

Alternate gene names: 16765356

Gene position: 2106002-2105211 (Counterclockwise)

Preceding gene: 16765357

Following gene: 16765355

Centisome position: 43.36

GC content: 59.47

Gene sequence:

>792_bases
GTGAATGAGGGGGATGTGCTGGTGGTGGGCGGCACCAGCGATGCGCGCGCGTTATGCCGACAACTGGATGCGGCGAACGT
CGCGTACACCTTATCCGTGGCGACGCCAGCCGGAAAAGCGCTGGCGGGCGACATTAAAGGGCAGGTGCGCTGTGGTCGTC
TGGAGTACGGGCAGATGGTCGCCTGGCTAAAAGAAAACCGTACTCGCTGGGTAATAGATGCATCGCATCCTTATGCAGAA
ATGGTCAGCCATAACCTCCTTCGCGCCTGTGAAACGGCAGGCGTGTTGTTAAGTCGTTATCAGCGTCCGGAGCAGCTTAG
CAATCTGACGCATCCGCTTCTCTATACGGCGCGCAGTATTGCCGATGCCTGCGAGATAGCGAGGCGTTTTGGTCCGCGCG
TGTTACTGACGACCGGCAGTAAAGATCTGGCCGTCTGGCGTGCCGGGCTGGCGGAAAAAACGTTGCTGGCGCGCGTACTG
CCCGTGGCGGAGGTGATTCAACGCTGCAGCGAACTGGGGTTCGGCGTTGGAGAAATTTTCGCTCTGTGTGGGCCGTTCAG
CGCTGATTTTAACGCCGCGTTTTACCACCAGTGCCGGGCCGACGTGGTCATTACCAAAGCGTCCGGCGCGGAGGGGGGAT
ATCAGGAAAAAGTTCAGCCCTGTCTGGATGCGGGCATTCCTTGCATCGTTATTGCGCGCCCGACGCCGCTTGTGACGGGT
GATGAGTTACTGGAAAGCCAGGCCGCCTTTGCGCAGCGTTTATCGCGCTGGCTGGCCGCAGCTAAGGAGTAA

Upstream 100 bases:

>100_bases
TGGATTTTGAACCGGTAGATATGACCAGTCTGGTCATCGTTGGTAATAAAACGACCTATGTTCAGGATGGGCTGATGATA
ACGCCGCGAGGTTACACGCT

Downstream 100 bases:

>100_bases
AAATGAAAAAAGCGCTTCTGGTGGTCAGTTTTGGCACCAGCTATCACGACACCTGTGAAAAAAACATTGTGGCGTGCGAA
CGTGATCTGGCGGCGTCCTG

Product: cobalt-precorrin-6x reductase

Products: Precorrin 6A; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MNEGDVLVVGGTSDARALCRQLDAANVAYTLSVATPAGKALAGDIKGQVRCGRLEYGQMVAWLKENRTRWVIDASHPYAE
MVSHNLLRACETAGVLLSRYQRPEQLSNLTHPLLYTARSIADACEIARRFGPRVLLTTGSKDLAVWRAGLAEKTLLARVL
PVAEVIQRCSELGFGVGEIFALCGPFSADFNAAFYHQCRADVVITKASGAEGGYQEKVQPCLDAGIPCIVIARPTPLVTG
DELLESQAAFAQRLSRWLAAAKE

Sequences:

>Translated_263_residues
MNEGDVLVVGGTSDARALCRQLDAANVAYTLSVATPAGKALAGDIKGQVRCGRLEYGQMVAWLKENRTRWVIDASHPYAE
MVSHNLLRACETAGVLLSRYQRPEQLSNLTHPLLYTARSIADACEIARRFGPRVLLTTGSKDLAVWRAGLAEKTLLARVL
PVAEVIQRCSELGFGVGEIFALCGPFSADFNAAFYHQCRADVVITKASGAEGGYQEKVQPCLDAGIPCIVIARPTPLVTG
DELLESQAAFAQRLSRWLAAAKE
>Mature_263_residues
MNEGDVLVVGGTSDARALCRQLDAANVAYTLSVATPAGKALAGDIKGQVRCGRLEYGQMVAWLKENRTRWVIDASHPYAE
MVSHNLLRACETAGVLLSRYQRPEQLSNLTHPLLYTARSIADACEIARRFGPRVLLTTGSKDLAVWRAGLAEKTLLARVL
PVAEVIQRCSELGFGVGEIFALCGPFSADFNAAFYHQCRADVVITKASGAEGGYQEKVQPCLDAGIPCIVIARPTPLVTG
DELLESQAAFAQRLSRWLAAAKE

Specific function: Catalyzes the reduction of the macrocycle of cobalt- precorrin-6X into cobalt-precorrin-6Y

COG id: COG2099

COG function: function code H; Precorrin-6x reductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin-6x reductase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CBIJ_SALTY (Q05591)

Other databases:

- EMBL:   L12006
- EMBL:   AE006468
- RefSeq:   NP_460971.1
- PRIDE:   Q05591
- GeneID:   1253547
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM2026
- NMPDR:   fig|99287.1.peg.1951
- HOGENOM:   HBG711334
- OMA:   PYIRFER
- ProtClustDB:   PRK08057
- BioCyc:   MetaCyc:MONOMER-13224
- BioCyc:   STYP99287:STM2026-MONOMER
- InterPro:   IPR003723
- TIGRFAMs:   TIGR00715

Pfam domain/function: PF02571 CbiJ

EC number: 1.3.1.54

Molecular weight: Translated: 28403; Mature: 28403

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS51014 COBK_CBIJ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEGDVLVVGGTSDARALCRQLDAANVAYTLSVATPAGKALAGDIKGQVRCGRLEYGQMV
CCCCCEEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHCCCCCCEEECCCHHHHHH
AWLKENRTRWVIDASHPYAEMVSHNLLRACETAGVLLSRYQRPEQLSNLTHPLLYTARSI
HHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
ADACEIARRFGPRVLLTTGSKDLAVWRAGLAEKTLLARVLPVAEVIQRCSELGFGVGEIF
HHHHHHHHHHCCEEEEEECCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ALCGPFSADFNAAFYHQCRADVVITKASGAEGGYQEKVQPCLDAGIPCIVIARPTPLVTG
HHHCCCCCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCH
DELLESQAAFAQRLSRWLAAAKE
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNEGDVLVVGGTSDARALCRQLDAANVAYTLSVATPAGKALAGDIKGQVRCGRLEYGQMV
CCCCCEEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHCCCCCCEEECCCHHHHHH
AWLKENRTRWVIDASHPYAEMVSHNLLRACETAGVLLSRYQRPEQLSNLTHPLLYTARSI
HHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
ADACEIARRFGPRVLLTTGSKDLAVWRAGLAEKTLLARVLPVAEVIQRCSELGFGVGEIF
HHHHHHHHHHCCEEEEEECCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ALCGPFSADFNAAFYHQCRADVVITKASGAEGGYQEKVQPCLDAGIPCIVIARPTPLVTG
HHHCCCCCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCH
DELLESQAAFAQRLSRWLAAAKE
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Precorrin 6B; NADP+

Specific reaction: precorrin-6B + NADP+ = precorrin-6A + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609