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The map label for this gene is cbiP

Identifier: 16765349

GI number: 16765349

Start: 2099682

End: 2101202

Strand: Reverse

Name: cbiP

Synonym: STM2019

Alternate gene names: 16765349

Gene position: 2101202-2099682 (Counterclockwise)

Preceding gene: 16765350

Following gene: 16765348

Centisome position: 43.26

GC content: 56.02

Gene sequence:

>1521_bases
ATGACGCAGGCAGTTATGTTGCAGGGGACGGCATCCGACGTCGGCAAAAGTGTGCTGGCGGCGGGTTTATGCCGCATTTT
TTATCAGGATGGTCTGCGTACCGCGCCGTTTAAATCGCAAAATATGGCGCTTAATTCTGGTATTACGCCGGATGGTAAAG
AGATGGGGCGGGCGCAAATTTTTCAGGCGGAAGCCGCGGGGATCACGCCAGATGTGCGTATGAACCCGGTGCTGCTCAAA
CCGACCAGCGATCGCCAGGCGCAGGTCGTGCTGATGGGGAAAGTGGCGACCAATATGGATGCGGTTAGTTACCATGACTA
TAAACCGCGTTTGCGTGAGCAAATCCTTGCGGTCTATAACAGCCTGGCGCAGGAATATGACGTTATTGTGCTGGAAGGCG
CCGGAAGCCCGGCGGAAATCAATCTGCGCGATCGCGATATCGTCAATATGGGAATGGCGGAAATGGCCCAGTGTCCGGTT
ATTCTGGTGGCGGATATCGACCGGGGCGGCGTATTCGCCGCTATTTATGGCACGCTGGCGCTGCTGCATAAACAGGAGCG
TGACAGAGTAAAGGGCGTCATTATCAACAAATTCCGCGGCGACGTGGCGCTACTCTATTCCGGTATCGAACAAATCGAAT
CGCTTACCGGCGTCCCGGTCCTGGGAGTGATGCCGTGGCTGGATGTGGATCTGGAAGATGAAGATGGCGTCGCGCTGCAA
AACGATAAATATCGCGGTAACGCCCCCCGTGATATCACTATTGCCATTGTGCAACTGCCACATATTTCTAACTTTACGGA
TTTTAACGCTCTGGCGGCGCAGCCGGATGTGCGCATACGCTATATTCGCCGCCCGGAAGCGTTGACTGACGTTGATCTGG
TGATCCTGCCGGGCAGTAAGAATACGCTGAGCGACCTCGCCTGGCTGCGCGAAAGCGGGATGGCGGATGCGGTATTGCAG
ACACATCGGCAGGGCGTGCCGGTGATGGGGATTTGCGGCGGCTACCAGATGCTGGGCGACACCATTGTGGATGAGGTGGA
GTCGGGGCTGGGTACGCAGCCGGGCCTGGGGCTGCTTAATACAATCACTCGCTTTGCACAGGATAAAACCACGACGCAGG
TTAACGCGACAATGTCAGGCGAGCTGCCCGGCTGGCTGGCTGCCGCGGCGGGGCTACCGGTACGCGGTTATGAAATTCAC
ATGGGGGAAACGGTGCTGCAGGAAGGATGCTGTACGGCCATGACGTTACAGAAAAATGGATGCTCTGTGGCGGATGGCGC
GGTCACCGCCGACGGACTGGCGTTTGGCACTTACCTTCACGGCCTGTTTGACAGCGATGCCTTTACCCGTGCGGTGGTCA
ATGGGTTACGGGCGCGAAAGGGGCTTGCGCCGTGGGAAACGACTTTTTGTTATGCGGATCATAAAGCGCGGCAATTTGAT
CTGCTGGCGGAAGCGATGCGGCAACATATTGATATTGATAAAATTTATACCATCATGCAACAACATCAGGAGCCGGTATG
A

Upstream 100 bases:

>100_bases
CCTGGCTGGTCAAACTTCATACTCAACTGGGACTGCCGCTGTGTAAAACAGAAACGGAGTTTTTTCATCGAATGCAAAAG
TGCGCATTCAGGGAGGCGTC

Downstream 100 bases:

>100_bases
TGATTCTGGTGACGGGCGGGGCACGTAGTGGTAAAAGCCGTCATGCTGAAGCCTTAATTGGCGATGCGCCGCAGGTACTG
TATATCGCCACCTCGCAGAT

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MTQAVMLQGTASDVGKSVLAAGLCRIFYQDGLRTAPFKSQNMALNSGITPDGKEMGRAQIFQAEAAGITPDVRMNPVLLK
PTSDRQAQVVLMGKVATNMDAVSYHDYKPRLREQILAVYNSLAQEYDVIVLEGAGSPAEINLRDRDIVNMGMAEMAQCPV
ILVADIDRGGVFAAIYGTLALLHKQERDRVKGVIINKFRGDVALLYSGIEQIESLTGVPVLGVMPWLDVDLEDEDGVALQ
NDKYRGNAPRDITIAIVQLPHISNFTDFNALAAQPDVRIRYIRRPEALTDVDLVILPGSKNTLSDLAWLRESGMADAVLQ
THRQGVPVMGICGGYQMLGDTIVDEVESGLGTQPGLGLLNTITRFAQDKTTTQVNATMSGELPGWLAAAAGLPVRGYEIH
MGETVLQEGCCTAMTLQKNGCSVADGAVTADGLAFGTYLHGLFDSDAFTRAVVNGLRARKGLAPWETTFCYADHKARQFD
LLAEAMRQHIDIDKIYTIMQQHQEPV

Sequences:

>Translated_506_residues
MTQAVMLQGTASDVGKSVLAAGLCRIFYQDGLRTAPFKSQNMALNSGITPDGKEMGRAQIFQAEAAGITPDVRMNPVLLK
PTSDRQAQVVLMGKVATNMDAVSYHDYKPRLREQILAVYNSLAQEYDVIVLEGAGSPAEINLRDRDIVNMGMAEMAQCPV
ILVADIDRGGVFAAIYGTLALLHKQERDRVKGVIINKFRGDVALLYSGIEQIESLTGVPVLGVMPWLDVDLEDEDGVALQ
NDKYRGNAPRDITIAIVQLPHISNFTDFNALAAQPDVRIRYIRRPEALTDVDLVILPGSKNTLSDLAWLRESGMADAVLQ
THRQGVPVMGICGGYQMLGDTIVDEVESGLGTQPGLGLLNTITRFAQDKTTTQVNATMSGELPGWLAAAAGLPVRGYEIH
MGETVLQEGCCTAMTLQKNGCSVADGAVTADGLAFGTYLHGLFDSDAFTRAVVNGLRARKGLAPWETTFCYADHKARQFD
LLAEAMRQHIDIDKIYTIMQQHQEPV
>Mature_505_residues
TQAVMLQGTASDVGKSVLAAGLCRIFYQDGLRTAPFKSQNMALNSGITPDGKEMGRAQIFQAEAAGITPDVRMNPVLLKP
TSDRQAQVVLMGKVATNMDAVSYHDYKPRLREQILAVYNSLAQEYDVIVLEGAGSPAEINLRDRDIVNMGMAEMAQCPVI
LVADIDRGGVFAAIYGTLALLHKQERDRVKGVIINKFRGDVALLYSGIEQIESLTGVPVLGVMPWLDVDLEDEDGVALQN
DKYRGNAPRDITIAIVQLPHISNFTDFNALAAQPDVRIRYIRRPEALTDVDLVILPGSKNTLSDLAWLRESGMADAVLQT
HRQGVPVMGICGGYQMLGDTIVDEVESGLGTQPGLGLLNTITRFAQDKTTTQVNATMSGELPGWLAAAAGLPVRGYEIHM
GETVLQEGCCTAMTLQKNGCSVADGAVTADGLAFGTYLHGLFDSDAFTRAVVNGLRARKGLAPWETTFCYADHKARQFDL
LAEAMRQHIDIDKIYTIMQQHQEPV

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CBIP_SALTY (Q05597)

Other databases:

- EMBL:   L12006
- EMBL:   AE006468
- RefSeq:   NP_460964.1
- ProteinModelPortal:   Q05597
- PRIDE:   Q05597
- GeneID:   1253540
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM2019
- NMPDR:   fig|99287.1.peg.1944
- HOGENOM:   HBG302271
- OMA:   CRTITEQ
- ProtClustDB:   PRK00784
- BioCyc:   MetaCyc:MONOMER-13223
- BioCyc:   STYP99287:STM2019-MONOMER
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 54968; Mature: 54837

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 332-332 ACT_SITE 440-440

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQAVMLQGTASDVGKSVLAAGLCRIFYQDGLRTAPFKSQNMALNSGITPDGKEMGRAQI
CCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCHHHHCHHHE
FQAEAAGITPDVRMNPVLLKPTSDRQAQVVLMGKVATNMDAVSYHDYKPRLREQILAVYN
EEHHCCCCCCCCCCCCEEECCCCCCCEEEEEEEEHHCCCCCEEECCCCHHHHHHHHHHHH
SLAQEYDVIVLEGAGSPAEINLRDRDIVNMGMAEMAQCPVILVADIDRGGVFAAIYGTLA
HHHHHCCEEEEECCCCCCEEEECCCHHHHCCHHHHHCCCEEEEEECCCCCCHHHHHHHHH
LLHKQERDRVKGVIINKFRGDVALLYSGIEQIESLTGVPVLGVMPWLDVDLEDEDGVALQ
HHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCEEEE
NDKYRGNAPRDITIAIVQLPHISNFTDFNALAAQPDVRIRYIRRPEALTDVDLVILPGSK
CCCCCCCCCCEEEEEEEEECCCCCCCCCCHHCCCCCCEEEEEECCCCCCCEEEEEECCCC
NTLSDLAWLRESGMADAVLQTHRQGVPVMGICGGYQMLGDTIVDEVESGLGTQPGLGLLN
CHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHH
TITRFAQDKTTTQVNATMSGELPGWLAAAAGLPVRGYEIHMGETVLQEGCCTAMTLQKNG
HHHHHHCCCCCEEEEEEECCCCCHHHHHHCCCCCCCEEEECCHHHHHCCCCEEEEEECCC
CSVADGAVTADGLAFGTYLHGLFDSDAFTRAVVNGLRARKGLAPWETTFCYADHKARQFD
CCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHH
LLAEAMRQHIDIDKIYTIMQQHQEPV
HHHHHHHHHCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TQAVMLQGTASDVGKSVLAAGLCRIFYQDGLRTAPFKSQNMALNSGITPDGKEMGRAQI
CCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCHHHHCHHHE
FQAEAAGITPDVRMNPVLLKPTSDRQAQVVLMGKVATNMDAVSYHDYKPRLREQILAVYN
EEHHCCCCCCCCCCCCEEECCCCCCCEEEEEEEEHHCCCCCEEECCCCHHHHHHHHHHHH
SLAQEYDVIVLEGAGSPAEINLRDRDIVNMGMAEMAQCPVILVADIDRGGVFAAIYGTLA
HHHHHCCEEEEECCCCCCEEEECCCHHHHCCHHHHHCCCEEEEEECCCCCCHHHHHHHHH
LLHKQERDRVKGVIINKFRGDVALLYSGIEQIESLTGVPVLGVMPWLDVDLEDEDGVALQ
HHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCEEEE
NDKYRGNAPRDITIAIVQLPHISNFTDFNALAAQPDVRIRYIRRPEALTDVDLVILPGSK
CCCCCCCCCCEEEEEEEEECCCCCCCCCCHHCCCCCCEEEEEECCCCCCCEEEEEECCCC
NTLSDLAWLRESGMADAVLQTHRQGVPVMGICGGYQMLGDTIVDEVESGLGTQPGLGLLN
CHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHH
TITRFAQDKTTTQVNATMSGELPGWLAAAAGLPVRGYEIHMGETVLQEGCCTAMTLQKNG
HHHHHHCCCCCEEEEEEECCCCCHHHHHHCCCCCCCEEEECCHHHHHCCCCEEEEEECCC
CSVADGAVTADGLAFGTYLHGLFDSDAFTRAVVNGLRARKGLAPWETTFCYADHKARQFD
CCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHH
LLAEAMRQHIDIDKIYTIMQQHQEPV
HHHHHHHHHCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609