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The map label for this gene is narX [H]

Identifier: 16765107

GI number: 16765107

Start: 1864282

End: 1866078

Strand: Direct

Name: narX [H]

Synonym: STM1766

Alternate gene names: 16765107

Gene position: 1864282-1866078 (Clockwise)

Preceding gene: 16765100

Following gene: 16765108

Centisome position: 38.38

GC content: 54.03

Gene sequence:

>1797_bases
ATGTTTAAACGGTGTTTCTCTCCTCTTACGCTGGTTAACCAACTGGCGCTGATTGTCATGCTCTCTACCGCTATCGGCGT
GGCGGGGATGGCGGTTTCCGGCTGGCTGGTTCAGGGTGTGCAGGGTAGCGCTCATGCGATTAATAAAGCGGGCTCATTGC
GTATGCAAAGCTATCGACTACTGGCAGCTGTCCCGCTGGATGCTAAAGATCAAAAGCTGCTGGATGAGATGGAGCAAACG
GCATTCAGCCCTGAACTGACCCGCGCCGCTGAGCGAGACGGGCAGCAGAAGCAGCTTAAAGCGTTGCAGGATTACTGGCA
TAACGAACTCTCGCCAGGGCTACAGCACGCGCAAAACGCGCACGCGGTCGCAGAAGATGTCACGCGCTTTGTGGCGGGCC
TGGATCGCCTGGTGACGTCGTTCGACCATACGACTGAATTGCGTATCGAACGCGTGGTATTAGTTCACCGGGTAATGGCG
ATCTTTATGGCGCTATTGCTGGTATTTACGATTATCTGGCTACGTGTTCGCCTTTTGCAGCCCTGGAAACAGTTGTTATC
AATGGCGCGCGCCGTCAGTCAGCGCGACTTTACCCAACGTGCCAATATCAGTGGACGCAATGAGATGGCCGCGTTGGGTT
CTGCGCTCAATAATATGTCTGAAGAACTGGCCGAAAGTTATGCGGTACTGGAACAGCGCGTACAGGAAAAAACAGCCGGT
CTGGAACATAAAAACCAGATTCTCTCGTTTTTGTGGCAAGCCAACCGCCGACTGCATTCACAGGCTCCGCTATGTGAGCG
TCTTTCACCGGTACTGAATGGCCTGCAAAATTTAACCCAGTTGCATGATATTGAGCTGCGGGTCTATGACCTGGAAGATG
AAGATAATCACCAGGAATTTACCTGTCAGTCGGACATCAGTTGCGATGATAAAGGCTGCCACCTTTGCCCACGTAGCGCC
CTGCCGATGATTAACGGCGGCACCACCTTAAAATGGCGGCTCACCGACTCTCACACCCAATACGGTATTTTGCTGGCGAC
GCTGCCCTATGGACGCCATCTCAGCCACGATCAACAACAGTTGGTGGACACGCTGGTTGAACAGCTTACCGCGACTCTGG
CGCTCGACAGGCATCAGGAGCGCCAGCAGCAGTTGATTGTCATGGAAGAGCGTGCCACCATTGCCCGCGAACTGCATGAT
TCTATTGCCCAGTCGCTCTCTTGTATGAAGATGCAGGTGAGCTGCCTGCAAATGCAGGGCGACGTGTTACCGGAAAGCAG
CCGTGAGTTACTCAGCCAAATTCGCAATGAGCTAAACTGCTCCTGGGCGCAACTGCGCGAACTGTTAACCACTTTCCGGT
TACAGTTGACGGAACCCGGGCTACGTCCGGCGCTGGAGGCAAGCTGCCAGGAGTTCAGCGCCCGGTTTGGCTTTACGGTA
AAACTGGATTATCAGCTTCCCCCCCGCCTGGTGCCTTCACACCAGGCGATACACCTGTTGCAAATTGCCAGGGAAGCCCT
CAGCAACGCCCTTAAACACTCTCATGCCGACGATGTGGTGGTGACAGTGACGCAATGTGGCAAACAGGTCAAACTAAAAG
TGCAGGATAATGGCTGCGGCGTCCCGGAAAACGCCGAACGCAGTAATCACTACGGCATGATCATCATGCGCGATCGGGCG
CAAAGTTTGCGCGGCGATTGCCAGGTACGCCGACGCGAGACTGGCGGAACAGAGGTCACTGTTACTTTTATTCCAGAAAC
AAACTTCACAGAAACCCAGGGAGATACCCATGAATAA

Upstream 100 bases:

>100_bases
TATACTCCGCTGTATCGTTGAGAATAGCGGCACAGCCGATTGACGCCCCTCTTTTTTGCTACGTTTTTTCGGCGACATTA
CCCCGAAGAAAGAAGGTAAC

Downstream 100 bases:

>100_bases
TCAGGAACCGGCAACCATCCTGTTAATCGATGACCATCCGATGCTACGCACGGGTGTAAAGCAGCTTGTGAGTATGGCGC
CCGATATCAGCGTGGTCGGA

Product: nitrate/nitrite sensor protein NarX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MFKRCFSPLTLVNQLALIVMLSTAIGVAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRLLAAVPLDAKDQKLLDEMEQT
AFSPELTRAAERDGQQKQLKALQDYWHNELSPGLQHAQNAHAVAEDVTRFVAGLDRLVTSFDHTTELRIERVVLVHRVMA
IFMALLLVFTIIWLRVRLLQPWKQLLSMARAVSQRDFTQRANISGRNEMAALGSALNNMSEELAESYAVLEQRVQEKTAG
LEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQNLTQLHDIELRVYDLEDEDNHQEFTCQSDISCDDKGCHLCPRSA
LPMINGGTTLKWRLTDSHTQYGILLATLPYGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERATIARELHD
SIAQSLSCMKMQVSCLQMQGDVLPESSRELLSQIRNELNCSWAQLRELLTTFRLQLTEPGLRPALEASCQEFSARFGFTV
KLDYQLPPRLVPSHQAIHLLQIAREALSNALKHSHADDVVVTVTQCGKQVKLKVQDNGCGVPENAERSNHYGMIIMRDRA
QSLRGDCQVRRRETGGTEVTVTFIPETNFTETQGDTHE

Sequences:

>Translated_598_residues
MFKRCFSPLTLVNQLALIVMLSTAIGVAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRLLAAVPLDAKDQKLLDEMEQT
AFSPELTRAAERDGQQKQLKALQDYWHNELSPGLQHAQNAHAVAEDVTRFVAGLDRLVTSFDHTTELRIERVVLVHRVMA
IFMALLLVFTIIWLRVRLLQPWKQLLSMARAVSQRDFTQRANISGRNEMAALGSALNNMSEELAESYAVLEQRVQEKTAG
LEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQNLTQLHDIELRVYDLEDEDNHQEFTCQSDISCDDKGCHLCPRSA
LPMINGGTTLKWRLTDSHTQYGILLATLPYGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERATIARELHD
SIAQSLSCMKMQVSCLQMQGDVLPESSRELLSQIRNELNCSWAQLRELLTTFRLQLTEPGLRPALEASCQEFSARFGFTV
KLDYQLPPRLVPSHQAIHLLQIAREALSNALKHSHADDVVVTVTQCGKQVKLKVQDNGCGVPENAERSNHYGMIIMRDRA
QSLRGDCQVRRRETGGTEVTVTFIPETNFTETQGDTHE
>Mature_598_residues
MFKRCFSPLTLVNQLALIVMLSTAIGVAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRLLAAVPLDAKDQKLLDEMEQT
AFSPELTRAAERDGQQKQLKALQDYWHNELSPGLQHAQNAHAVAEDVTRFVAGLDRLVTSFDHTTELRIERVVLVHRVMA
IFMALLLVFTIIWLRVRLLQPWKQLLSMARAVSQRDFTQRANISGRNEMAALGSALNNMSEELAESYAVLEQRVQEKTAG
LEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQNLTQLHDIELRVYDLEDEDNHQEFTCQSDISCDDKGCHLCPRSA
LPMINGGTTLKWRLTDSHTQYGILLATLPYGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERATIARELHD
SIAQSLSCMKMQVSCLQMQGDVLPESSRELLSQIRNELNCSWAQLRELLTTFRLQLTEPGLRPALEASCQEFSARFGFTV
KLDYQLPPRLVPSHQAIHLLQIAREALSNALKHSHADDVVVTVTQCGKQVKLKVQDNGCGVPENAERSNHYGMIIMRDRA
QSLRGDCQVRRRETGGTEVTVTFIPETNFTETQGDTHE

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate availability to the narL protein and of both nitrate/nitrite to the narP protein. NarX probably activates narL and narP by phosphorylation in the presence of nitrate. NarX also plays a

COG id: COG3850

COG function: function code T; Signal transduction histidine kinase, nitrate/nitrite-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=598, Percent_Identity=86.1204013377926, Blast_Score=1064, Evalue=0.0,
Organism=Escherichia coli, GI1788812, Length=586, Percent_Identity=30.0341296928328, Blast_Score=194, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR003661
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 67439; Mature: 67439

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKRCFSPLTLVNQLALIVMLSTAIGVAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRL
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHE
LAAVPLDAKDQKLLDEMEQTAFSPELTRAAERDGQQKQLKALQDYWHNELSPGLQHAQNA
EEECCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
HAVAEDVTRFVAGLDRLVTSFDHTTELRIERVVLVHRVMAIFMALLLVFTIIWLRVRLLQ
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PWKQLLSMARAVSQRDFTQRANISGRNEMAALGSALNNMSEELAESYAVLEQRVQEKTAG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQNLTQLHDIELRVYDLEDEDNHQEF
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCE
TCQSDISCDDKGCHLCPRSALPMINGGTTLKWRLTDSHTQYGILLATLPYGRHLSHDQQQ
EECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCHHHH
LVDTLVEQLTATLALDRHQERQQQLIVMEERATIARELHDSIAQSLSCMKMQVSCLQMQG
HHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DVLPESSRELLSQIRNELNCSWAQLRELLTTFRLQLTEPGLRPALEASCQEFSARFGFTV
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEE
KLDYQLPPRLVPSHQAIHLLQIAREALSNALKHSHADDVVVTVTQCGKQVKLKVQDNGCG
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCEEEEEECCCCCC
VPENAERSNHYGMIIMRDRAQSLRGDCQVRRRETGGTEVTVTFIPETNFTETQGDTHE
CCCCCCCCCCCCEEEECCCHHHHHCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MFKRCFSPLTLVNQLALIVMLSTAIGVAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRL
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHE
LAAVPLDAKDQKLLDEMEQTAFSPELTRAAERDGQQKQLKALQDYWHNELSPGLQHAQNA
EEECCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
HAVAEDVTRFVAGLDRLVTSFDHTTELRIERVVLVHRVMAIFMALLLVFTIIWLRVRLLQ
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PWKQLLSMARAVSQRDFTQRANISGRNEMAALGSALNNMSEELAESYAVLEQRVQEKTAG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQNLTQLHDIELRVYDLEDEDNHQEF
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCE
TCQSDISCDDKGCHLCPRSALPMINGGTTLKWRLTDSHTQYGILLATLPYGRHLSHDQQQ
EECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCHHHH
LVDTLVEQLTATLALDRHQERQQQLIVMEERATIARELHDSIAQSLSCMKMQVSCLQMQG
HHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DVLPESSRELLSQIRNELNCSWAQLRELLTTFRLQLTEPGLRPALEASCQEFSARFGFTV
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEE
KLDYQLPPRLVPSHQAIHLLQIAREALSNALKHSHADDVVVTVTQCGKQVKLKVQDNGCG
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCEEEEEECCCCCC
VPENAERSNHYGMIIMRDRAQSLRGDCQVRRRETGGTEVTVTFIPETNFTETQGDTHE
CCCCCCCCCCCCEEEECCCHHHHHCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]