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The map label for this gene is ydaO [H]

Identifier: 16764998

GI number: 16764998

Start: 1747309

End: 1748244

Strand: Direct

Name: ydaO [H]

Synonym: STM1654

Alternate gene names: 16764998

Gene position: 1747309-1748244 (Clockwise)

Preceding gene: 16764996

Following gene: 16765003

Centisome position: 35.97

GC content: 50.53

Gene sequence:

>936_bases
ATGCAAGAAATTCAAAAGAATACAAAGAAAGAGCAATACAACCTCAACAAGTTGCAAAAGCGCCTGCGCCGTAACGTTGG
CGAAGCGATTGCCGATTTTAATATGATTGAAGAAGGCGATCGCATTATGGTTTGCCTTTCTGGCGGCAAAGATAGCTATA
CGATGCTGGAAATTTTACGTAATTTGCAGCAAAGCGCCCCGATCAATTTTTCACTGGTCGCCGTCAACCTCGATCAAAAG
CAGCCAGGTTTCCCGGAACATATCCTGCCAGCCTACCTTGAGCAGCTGGGCGTAGAATATAAAATCGTCGAAGAAAACAC
CTACGGCATTGTGAAAGAGAAGATTCCGGAAGGAAAAACCACCTGCTCGCTGTGCTCGCGTTTGCGTCGGGGTATCCTGT
ATCGTACGGCGACTGAACTGGGCGCGACCAAAATCGCCCTGGGCCACCACCGCGACGATATTCTGCAAACCCTGTTTCTG
AATATGTTCTATGGCGGAAAAATGAAAGGGATGCCGCCGAAGCTGATGAGCGATGACGGCAAACATATCGTGATCCGCCC
GCTGGCTTACTGCCGCGAGAAAGATATTATCCGTTTTGCTGAGGCCAAAGCCTTCCCTATCATTCCTTGTAATCTGTGCG
GTTCGCAACCAAACCTGCAACGCCAGGTGATTGCCGACATGCTACGCGACTGGGATAAGCGCTATCCTGGACGGATCGAG
ACGATGTTTAGCGCCATGCAGAATGTCGTGCCGTCTCACCTTTGTGACACTAACCTGTTCGATTTCAAAGGAATCACTCA
CGGTTCCGAGGTCGTCGACGGCGGCGATTTAGCGTTCGATCGTGAAGAGATTCCCTTGCAGCCCGCTGGCTGGCAGCCGG
AAGAAGATGACACCGCCTTAGAGGCGTTGCGGCTTGATGTTATCGAAGTGAAATAA

Upstream 100 bases:

>100_bases
TGACATGAACGACGAACAAGTAGCGATGTTGAATGCGCGGTTATCGTAATTGCAAAGTTTGCCCCCAATTTGCCCCATTT
AGTACCAGAGAACTGAAATA

Downstream 100 bases:

>100_bases
TCTGCAGGCGTCTCAGCACTCCGCTGAGACGCCATGCTATCGATCATTTTAATAGCCGTACCCGGCATGACTTGCCTTTG
ATCTTCCCGTTTTGCAACTG

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MQEIQKNTKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMVCLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQK
QPGFPEHILPAYLEQLGVEYKIVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIIRFAEAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIE
TMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVDGGDLAFDREEIPLQPAGWQPEEDDTALEALRLDVIEVK

Sequences:

>Translated_311_residues
MQEIQKNTKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMVCLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQK
QPGFPEHILPAYLEQLGVEYKIVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIIRFAEAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIE
TMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVDGGDLAFDREEIPLQPAGWQPEEDDTALEALRLDVIEVK
>Mature_311_residues
MQEIQKNTKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMVCLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQK
QPGFPEHILPAYLEQLGVEYKIVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIIRFAEAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIE
TMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVDGGDLAFDREEIPLQPAGWQPEEDDTALEALRLDVIEVK

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Escherichia coli, GI1787606, Length=311, Percent_Identity=94.5337620578778, Blast_Score=615, Evalue=1e-177,
Organism=Caenorhabditis elegans, GI193211090, Length=250, Percent_Identity=31.6, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17540090, Length=235, Percent_Identity=27.2340425531915, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24651972, Length=234, Percent_Identity=26.9230769230769, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 35358; Mature: 35358

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEIQKNTKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMVCLSGGKDSYTMLEILR
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHH
NLQQSAPINFSLVAVNLDQKQPGFPEHILPAYLEQLGVEYKIVEENTYGIVKEKIPEGKT
HHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHCCCCCH
TCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFLNMFYGGKMKGMPPKLMSDDG
HHHHHHHHHHHHHHHHHHHHCCHHEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC
KHIVIRPLAYCREKDIIRFAEAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIE
CEEEECCHHHHCHHHHHHHHHCCCCCEEEHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
TMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVDGGDLAFDREEIPLQPAGWQPEEDDTAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
EALRLDVIEVK
HHHHHHHEECC
>Mature Secondary Structure
MQEIQKNTKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMVCLSGGKDSYTMLEILR
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHH
NLQQSAPINFSLVAVNLDQKQPGFPEHILPAYLEQLGVEYKIVEENTYGIVKEKIPEGKT
HHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHCCCCCH
TCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFLNMFYGGKMKGMPPKLMSDDG
HHHHHHHHHHHHHHHHHHHHCCHHEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC
KHIVIRPLAYCREKDIIRFAEAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIE
CEEEECCHHHHCHHHHHHHHHCCCCCEEEHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
TMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVDGGDLAFDREEIPLQPAGWQPEEDDTAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
EALRLDVIEVK
HHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA