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The map label for this gene is narY [H]

Identifier: 16764922

GI number: 16764922

Start: 1664425

End: 1665969

Strand: Direct

Name: narY [H]

Synonym: STM1578

Alternate gene names: 16764922

Gene position: 1664425-1665969 (Clockwise)

Preceding gene: 16764921

Following gene: 16764923

Centisome position: 34.27

GC content: 54.11

Gene sequence:

>1545_bases
ATGAAAATACGCTCACAGGTTGGGATGGTACTGAATCTCGATAAATGTATTGGCTGTCACACCTGCTCCGTCACCTGTAA
AAACGTCTGGACCGGACGTGAAGGGATGGAATACGCATGGTTCAATAACGTCGAAACTAAACCAGGCATCGGCTATCCCA
AAAACTGGGAGGACCAGCAGGAGTGGCAAGGCGGTTGGGTTCGTGATGTTAATGGCAAGATAAGGCCCCGTCTGGGCGGC
AAAATGGGGGTAATTAGCAAAATTTTCGCCAACCCGGTGATTCCGCAAATCGATGATTACTATGAACCCTTTACCTTCGA
TTATCAGCATTTACATAATGCGCCGGAGAGTAAGCATCAGCCCACTGCCCGCCCTCGTTCACTGATCGACGGAAAACGGA
TGGACAAGGTGATCTGGGGGCCAAACTGGGAAGAGCTGCTCGGCGGCGAGTTTGAAAAACGCGCCCGCGACCGCAACTTC
GACAACATACAAAAAGAGATGTACGGTCAGTTCGAAAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCT
GAACCCCAGTTGTGTCGCCACCTGCCCAAGTGGCGCTATTTATAAGCGTGAAGAAGACGGCATCGTGCTAATCGACCAGG
ATAAATGCCGAGGCTGGCGTTTGTGTATCAGCGGCTGCCCGTACAAAAAAATCTACTTTAACTGGAAGAGCGGCAAGTCT
GAGAAGTGCATATTCTGCTATCCGCGTATTGAATCCGGCCAGCCGACCGTTTGCTCGGAAACCTGCGTCGGGCGCATTCG
TTATCTTGGCGTTCTGCTCTACGACGCCGACAGGATTGAAGAAGCGGCCAGTACCGAACACGAGACGGATTTGTATGAAC
GTCAGTGCGATGTGTTTCTCAATCCGCACGATCCGGCGGTCATCGAAGAGGCGCTTAAACAAGGCATCCCGCAAAACGTC
ATCGACGCCGCCCAACGGTCGCCGGTGTACAAAATGGCGATGGACTGGAAGCTGGCGCTTCCGCTGCACCCGGAATACCG
TACCCTGCCGATGGTCTGGTACGTACCGCCGCTATCGCCCATCCAGTCATACGCTGACGCGGGCGGGTTGCCGCACAATG
GCAATATTCTGCCGGCGGTGGAAACGCTGCGTATTCCAGTCCAGTACCTGGCCAATATGCTCAGCGCGGGCGATACCGGC
CCGGTCATTCGGGCGCTAAAGCGGATGATGGCGATGCGCCACTACATGCGCTCGCAGACGGTTGAAGGCGTGACGGATAC
CCGCGCCATTGAGGAAGTCGGTCTGAGCATACAACAGGTTGAAGAGATGTATCGCTACCTGGCGATTGCCAATTATGAAG
ATCGTTTTGTCATTCCCACCAGCCACCGGGAAATGGCGCGCGATGCCTTTCCGGAGCGCAACGGCTGCGGCTTTACTTTT
GGCGACGGTTGCCACGGGTCGGATACCAAATTCAACCTGTTCAACAGTAGCCGTATTGATGCCATCAATATCACCGAAGT
ACGTGATAAAGCGGAGGGGGAATAA

Upstream 100 bases:

>100_bases
CCGTGGGTTCGAATCGCGATGAATTCATCATGATCAGGAAGATGAAAAACATTAACTGGCTGGATGATGAGGGCCGGGAT
CAGGTACAGGAGGCGAAAAA

Downstream 100 bases:

>100_bases
TGCAAATCCTCAAAGCGATTGGCCTGTTGATGGAGTATCCGGACGACGAGTTGTGGGAATGTCGGGATGAAGCGCTCACC
CTTATCCAACACGATGCGCC

Product: nitrate reductase 2 beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQQEWQGGWVRDVNGKIRPRLGG
KMGVISKIFANPVIPQIDDYYEPFTFDYQHLHNAPESKHQPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
DNIQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEHETDLYERQCDVFLNPHDPAVIEEALKQGIPQNV
IDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPHNGNILPAVETLRIPVQYLANMLSAGDTG
PVIRALKRMMAMRHYMRSQTVEGVTDTRAIEEVGLSIQQVEEMYRYLAIANYEDRFVIPTSHREMARDAFPERNGCGFTF
GDGCHGSDTKFNLFNSSRIDAINITEVRDKAEGE

Sequences:

>Translated_514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQQEWQGGWVRDVNGKIRPRLGG
KMGVISKIFANPVIPQIDDYYEPFTFDYQHLHNAPESKHQPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
DNIQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEHETDLYERQCDVFLNPHDPAVIEEALKQGIPQNV
IDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPHNGNILPAVETLRIPVQYLANMLSAGDTG
PVIRALKRMMAMRHYMRSQTVEGVTDTRAIEEVGLSIQQVEEMYRYLAIANYEDRFVIPTSHREMARDAFPERNGCGFTF
GDGCHGSDTKFNLFNSSRIDAINITEVRDKAEGE
>Mature_514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQQEWQGGWVRDVNGKIRPRLGG
KMGVISKIFANPVIPQIDDYYEPFTFDYQHLHNAPESKHQPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
DNIQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEHETDLYERQCDVFLNPHDPAVIEEALKQGIPQNV
IDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPHNGNILPAVETLRIPVQYLANMLSAGDTG
PVIRALKRMMAMRHYMRSQTVEGVTDTRAIEEVGLSIQQVEEMYRYLAIANYEDRFVIPTSHREMARDAFPERNGCGFTF
GDGCHGSDTKFNLFNSSRIDAINITEVRDKAEGE

Specific function: This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in t

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=514, Percent_Identity=94.1634241245136, Blast_Score=1029, Evalue=0.0,
Organism=Escherichia coli, GI1787478, Length=506, Percent_Identity=80.2371541501976, Blast_Score=877, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=99, Percent_Identity=42.4242424242424, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787122, Length=99, Percent_Identity=42.4242424242424, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI2367345, Length=111, Percent_Identity=30.6306306306306, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789370, Length=99, Percent_Identity=37.3737373737374, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI226510944, Length=86, Percent_Identity=36.046511627907, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=34.4827586206897, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 58794; Mature: 58794

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQQ
CCCHHHCCCEEEHHHHHCCCEEEEEHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHH
EWQGGWVRDVNGKIRPRLGGKMGVISKIFANPVIPQIDDYYEPFTFDYQHLHNAPESKHQ
HHCCCCEEECCCEECCCCCCCHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHCCCCCCCCC
PTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNFDNIQKEMYGQFENTFMMYLP
CCCCCHHHHCCHHHCCEEECCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
HHHHHHCCCCEEEECCCCCEEEECCCCEEEEECCCCCCEEEEECCCCCEEEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEHETDLYERQCDVFL
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHCCCHHHHHHHHCCCEEE
NPHDPAVIEEALKQGIPQNVIDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSP
CCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCCEEEECCCCHH
IQSYADAGGLPHNGNILPAVETLRIPVQYLANMLSAGDTGPVIRALKRMMAMRHYMRSQT
HHHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VEGVTDTRAIEEVGLSIQQVEEMYRYLAIANYEDRFVIPTSHREMARDAFPERNGCGFTF
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHCCCCCCCCCCCC
GDGCHGSDTKFNLFNSSRIDAINITEVRDKAEGE
CCCCCCCCCEEEEECCCCCCEEEHHHHHHHCCCC
>Mature Secondary Structure
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQQ
CCCHHHCCCEEEHHHHHCCCEEEEEHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHH
EWQGGWVRDVNGKIRPRLGGKMGVISKIFANPVIPQIDDYYEPFTFDYQHLHNAPESKHQ
HHCCCCEEECCCEECCCCCCCHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHCCCCCCCCC
PTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNFDNIQKEMYGQFENTFMMYLP
CCCCCHHHHCCHHHCCEEECCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
HHHHHHCCCCEEEECCCCCEEEECCCCEEEEECCCCCCEEEEECCCCCEEEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEHETDLYERQCDVFL
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHCCCHHHHHHHHCCCEEE
NPHDPAVIEEALKQGIPQNVIDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSP
CCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCCEEEECCCCHH
IQSYADAGGLPHNGNILPAVETLRIPVQYLANMLSAGDTGPVIRALKRMMAMRHYMRSQT
HHHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VEGVTDTRAIEEVGLSIQQVEEMYRYLAIANYEDRFVIPTSHREMARDAFPERNGCGFTF
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHCCCCCCCCCCCC
GDGCHGSDTKFNLFNSSRIDAINITEVRDKAEGE
CCCCCCCCCEEEEECCCCCCEEEHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2233673; 9097039; 9278503 [H]