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The map label for this gene is glgX [H]

Identifier: 16764902

GI number: 16764902

Start: 1636060

End: 1638135

Strand: Reverse

Name: glgX [H]

Synonym: STM1558

Alternate gene names: 16764902

Gene position: 1638135-1636060 (Counterclockwise)

Preceding gene: 16764903

Following gene: 16764898

Centisome position: 33.72

GC content: 52.12

Gene sequence:

>2076_bases
ATGACAACAAACAGGGCTTTTGAAATCAGATCAGGATACAGCCATACGCTTGGCGCAAATTATGACGGCGAAGGCGTGAA
TTTCGCTATTTTTTCCGCCCATGCGGAACGCGTCGAGCTTTGCCTTTACGATCCGAGCGGGGAGCATGAAATCGCTCGTC
TGGAGTTACCGGAATATACTGACGAAATATGGCACGGCTATGTCCCGAAGTTGCAGCCCGGCGCGCTGTATGGGTACCGC
GTTTACGGTCCTTATGACCCGGAAAATGGTCATCGTTTTAACCCCAACAAGCTGCTTATTGACCCTTATGCCCGTGAGCT
GGTGGGCGATATTCAGTGGAACGATGCGCATTTCGCGTATCAATTACTTCATGACGATAAAGATTTGACCTTTGATGACC
AGGATAGCGCGCCATTTACGCCTAAATGCCGGGTGATTGATCCGGATGAGGCTAACTGGGAGGATAGACAACGGCCTTCC
ATACCGTGGTCGAACGCCATTATTTATGAAACGCATGTAAAAGGCTTTACCCAGCTTAATTCCGCGATCCCTGAGCCTCT
ACGCGGTACGTTTGAAGGCATGGGGCATAAAGCCAGCGTGGATTACATTAAAAGTCTGGGGATTACCTCCGTCGAGCTTT
TACCCGTTCACTGGTTCCCGGACGATCAGCATCTGCTCGATAAAGGACTGAAAAATTTCTGGGGATACAACTCGCTGGGC
TTTTTTGCGCCGGCGACGCGCTACTATGGTCCAAAAGGTATTCAGGGATTCCGCGATATGGTGCGCGCTTTCCATGATGC
CGGCATCGAAGTGATACTGGATGTGGTCTATAACCATACGGCAGAAGGGAATGAACTTGGCCCCACGCTGTCATTTAAAG
GTATCGATAACTTCTCTTACTATCGCACGATGCCCGATCAGCATCGCTATTACATTAACGATACAGGGACAGGTAATACC
GTCAATACCTCTCATCCACGGGTACTGCAAATGGTGATGGATTCCCTTCGCTATTGGGCGGAGTCAATGCATGTCGACGG
CTTTCGTTTTGATTTGGGCACCATTCTTGGTCGTGAGCCGGAAGGCTTTGATCAGAGAGGCGGTTTTTTTGATGCGGTAA
CGCAAGACCCGGTGCTGGCAAAACTGAAACTGATTGGCGAACCCTGGGATATCGGACCGGGCGGCTATCAGGTTGGCGGC
TTTCCACCAGGGTGGGGCGAATGGAATGATAAATACCGCGATACGGTACGCGAATACTGGAAAGGTGACAACGTCACAAA
TGATTTCGCCGCACGCCTGCTGGGTTCCGGCGATCTCTATGATTTGCGCGGCCGTCGCCCCTGGTCAAGCGTTAACTTTA
TTACTGCCCATGACGGCTTCACGCTGAACGATCTGGTGTCTTATAACGACAAACATAATGAAGCCAATGGCGAAGATAAT
AATGACGGGCATAATGATAACCGCTCCTGTAACTATGGCGCAGAAGGGCCAACCGACGACGAGGGCATCAATGCGATACG
CGAGCGACAGAAACGTAATTTCCTGACCACCTTGCTTTTCTCGCACGGCACGCCGATGTTATTAGCGGGCGATGAGTTTG
GCCGCAGTCAGATGGGCAATAACAATGGCTACTGTCAGGACAGTGAAATATCGTGGGTCCACTGGGATAATTTGCCGGAA
ACCGCCAATGCGCTACGGGAGTTTACCCGTCATCTGATTCAACTGCGCGCCACGCAACCGTTGCTGCGCCGGGAGAGCTG
GCGTGACGGTCTGGAGATCCGCTGGTTTAACGCCGGCGGAGGGGCGCAGCAATCCGAACAATGGGATGAGGGATCGACCA
TAGGCGTATGTATTAGTCGTCCCGATCTTCAGCCGGAAGCGGGGATCTGGCATGACGCCTTACTGCTTTTCAATCCGTTT
GAAGGCAGCGTACCGTTCCGTATTCCGATGTGGGGAGAAGGCGGCTGGGTACTTGAACTGACGACTGCCGATAACGCGCA
GCAGGGAATGCGGATTACCGAAGAAATGGATTTCGATCTGGCCGGGCGCAGTATCGTGTTATTCAGACGCCCCTGA

Upstream 100 bases:

>100_bases
TGGCCTCACACCAGGGCGGCGTACTGGTTTTAATGAGCGACTGAGTTTATGTGTTGATGTGCCGTGACAGGACAACGTTT
ACCGTGGGTGGAGAAAGAGA

Downstream 100 bases:

>100_bases
TAGTAAAACCGCGCTCCGGACCCAACCGGCCTTGATGGCCGGTTTTTTACTGGCGCTCAGGCGATATCATGCCGCAGAAA
TAACACCGTTGTCAGTTTAC

Product: glycosyl hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 691; Mature: 690

Protein sequence:

>691_residues
MTTNRAFEIRSGYSHTLGANYDGEGVNFAIFSAHAERVELCLYDPSGEHEIARLELPEYTDEIWHGYVPKLQPGALYGYR
VYGPYDPENGHRFNPNKLLIDPYARELVGDIQWNDAHFAYQLLHDDKDLTFDDQDSAPFTPKCRVIDPDEANWEDRQRPS
IPWSNAIIYETHVKGFTQLNSAIPEPLRGTFEGMGHKASVDYIKSLGITSVELLPVHWFPDDQHLLDKGLKNFWGYNSLG
FFAPATRYYGPKGIQGFRDMVRAFHDAGIEVILDVVYNHTAEGNELGPTLSFKGIDNFSYYRTMPDQHRYYINDTGTGNT
VNTSHPRVLQMVMDSLRYWAESMHVDGFRFDLGTILGREPEGFDQRGGFFDAVTQDPVLAKLKLIGEPWDIGPGGYQVGG
FPPGWGEWNDKYRDTVREYWKGDNVTNDFAARLLGSGDLYDLRGRRPWSSVNFITAHDGFTLNDLVSYNDKHNEANGEDN
NDGHNDNRSCNYGAEGPTDDEGINAIRERQKRNFLTTLLFSHGTPMLLAGDEFGRSQMGNNNGYCQDSEISWVHWDNLPE
TANALREFTRHLIQLRATQPLLRRESWRDGLEIRWFNAGGGAQQSEQWDEGSTIGVCISRPDLQPEAGIWHDALLLFNPF
EGSVPFRIPMWGEGGWVLELTTADNAQQGMRITEEMDFDLAGRSIVLFRRP

Sequences:

>Translated_691_residues
MTTNRAFEIRSGYSHTLGANYDGEGVNFAIFSAHAERVELCLYDPSGEHEIARLELPEYTDEIWHGYVPKLQPGALYGYR
VYGPYDPENGHRFNPNKLLIDPYARELVGDIQWNDAHFAYQLLHDDKDLTFDDQDSAPFTPKCRVIDPDEANWEDRQRPS
IPWSNAIIYETHVKGFTQLNSAIPEPLRGTFEGMGHKASVDYIKSLGITSVELLPVHWFPDDQHLLDKGLKNFWGYNSLG
FFAPATRYYGPKGIQGFRDMVRAFHDAGIEVILDVVYNHTAEGNELGPTLSFKGIDNFSYYRTMPDQHRYYINDTGTGNT
VNTSHPRVLQMVMDSLRYWAESMHVDGFRFDLGTILGREPEGFDQRGGFFDAVTQDPVLAKLKLIGEPWDIGPGGYQVGG
FPPGWGEWNDKYRDTVREYWKGDNVTNDFAARLLGSGDLYDLRGRRPWSSVNFITAHDGFTLNDLVSYNDKHNEANGEDN
NDGHNDNRSCNYGAEGPTDDEGINAIRERQKRNFLTTLLFSHGTPMLLAGDEFGRSQMGNNNGYCQDSEISWVHWDNLPE
TANALREFTRHLIQLRATQPLLRRESWRDGLEIRWFNAGGGAQQSEQWDEGSTIGVCISRPDLQPEAGIWHDALLLFNPF
EGSVPFRIPMWGEGGWVLELTTADNAQQGMRITEEMDFDLAGRSIVLFRRP
>Mature_690_residues
TTNRAFEIRSGYSHTLGANYDGEGVNFAIFSAHAERVELCLYDPSGEHEIARLELPEYTDEIWHGYVPKLQPGALYGYRV
YGPYDPENGHRFNPNKLLIDPYARELVGDIQWNDAHFAYQLLHDDKDLTFDDQDSAPFTPKCRVIDPDEANWEDRQRPSI
PWSNAIIYETHVKGFTQLNSAIPEPLRGTFEGMGHKASVDYIKSLGITSVELLPVHWFPDDQHLLDKGLKNFWGYNSLGF
FAPATRYYGPKGIQGFRDMVRAFHDAGIEVILDVVYNHTAEGNELGPTLSFKGIDNFSYYRTMPDQHRYYINDTGTGNTV
NTSHPRVLQMVMDSLRYWAESMHVDGFRFDLGTILGREPEGFDQRGGFFDAVTQDPVLAKLKLIGEPWDIGPGGYQVGGF
PPGWGEWNDKYRDTVREYWKGDNVTNDFAARLLGSGDLYDLRGRRPWSSVNFITAHDGFTLNDLVSYNDKHNEANGEDNN
DGHNDNRSCNYGAEGPTDDEGINAIRERQKRNFLTTLLFSHGTPMLLAGDEFGRSQMGNNNGYCQDSEISWVHWDNLPET
ANALREFTRHLIQLRATQPLLRRESWRDGLEIRWFNAGGGAQQSEQWDEGSTIGVCISRPDLQPEAGIWHDALLLFNPFE
GSVPFRIPMWGEGGWVLELTTADNAQQGMRITEEMDFDLAGRSIVLFRRP

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=610, Percent_Identity=48.3606557377049, Blast_Score=556, Evalue=1e-159,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 78570; Mature: 78439

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNRAFEIRSGYSHTLGANYDGEGVNFAIFSAHAERVELCLYDPSGEHEIARLELPEYT
CCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCEEEEEECCHHH
DEIWHGYVPKLQPGALYGYRVYGPYDPENGHRFNPNKLLIDPYARELVGDIQWNDAHFAY
HHHHCCCCCCCCCCCEEEEEEECCCCCCCCCEECCCEEEECHHHHHHHHCCEECCHHEEE
QLLHDDKDLTFDDQDSAPFTPKCRVIDPDEANWEDRQRPSIPWSNAIIYETHVKGFTQLN
EEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHH
SAIPEPLRGTFEGMGHKASVDYIKSLGITSVELLPVHWFPDDQHLLDKGLKNFWGYNSLG
HHCCHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCCC
FFAPATRYYGPKGIQGFRDMVRAFHDAGIEVILDVVYNHTAEGNELGPTLSFKGIDNFSY
CCCCHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHH
YRTMPDQHRYYINDTGTGNTVNTSHPRVLQMVMDSLRYWAESMHVDGFRFDLGTILGREP
EECCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCC
EGFDQRGGFFDAVTQDPVLAKLKLIGEPWDIGPGGYQVGGFPPGWGEWNDKYRDTVREYW
CCCCCCCCEECCCCCCCCEEEHEECCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHH
KGDNVTNDFAARLLGSGDLYDLRGRRPWSSVNFITAHDGFTLNDLVSYNDKHNEANGEDN
CCCCCCHHHHHHHHCCCCEEECCCCCCCCCCCEEEECCCCCHHHHHHCCCCCCCCCCCCC
NDGHNDNRSCNYGAEGPTDDEGINAIRERQKRNFLTTLLFSHGTPMLLAGDEFGRSQMGN
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHCHHHCCC
NNGYCQDSEISWVHWDNLPETANALREFTRHLIQLRATQPLLRRESWRDGLEIRWFNAGG
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCC
GAQQSEQWDEGSTIGVCISRPDLQPEAGIWHDALLLFNPFEGSVPFRIPMWGEGGWVLEL
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEEE
TTADNAQQGMRITEEMDFDLAGRSIVLFRRP
ECCCCCHHCCEEHHHCCCCCCCCEEEEEECC
>Mature Secondary Structure 
TTNRAFEIRSGYSHTLGANYDGEGVNFAIFSAHAERVELCLYDPSGEHEIARLELPEYT
CCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCEEEEEECCHHH
DEIWHGYVPKLQPGALYGYRVYGPYDPENGHRFNPNKLLIDPYARELVGDIQWNDAHFAY
HHHHCCCCCCCCCCCEEEEEEECCCCCCCCCEECCCEEEECHHHHHHHHCCEECCHHEEE
QLLHDDKDLTFDDQDSAPFTPKCRVIDPDEANWEDRQRPSIPWSNAIIYETHVKGFTQLN
EEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHH
SAIPEPLRGTFEGMGHKASVDYIKSLGITSVELLPVHWFPDDQHLLDKGLKNFWGYNSLG
HHCCHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCCC
FFAPATRYYGPKGIQGFRDMVRAFHDAGIEVILDVVYNHTAEGNELGPTLSFKGIDNFSY
CCCCHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHH
YRTMPDQHRYYINDTGTGNTVNTSHPRVLQMVMDSLRYWAESMHVDGFRFDLGTILGREP
EECCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCC
EGFDQRGGFFDAVTQDPVLAKLKLIGEPWDIGPGGYQVGGFPPGWGEWNDKYRDTVREYW
CCCCCCCCEECCCCCCCCEEEHEECCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHH
KGDNVTNDFAARLLGSGDLYDLRGRRPWSSVNFITAHDGFTLNDLVSYNDKHNEANGEDN
CCCCCCHHHHHHHHCCCCEEECCCCCCCCCCCEEEECCCCCHHHHHHCCCCCCCCCCCCC
NDGHNDNRSCNYGAEGPTDDEGINAIRERQKRNFLTTLLFSHGTPMLLAGDEFGRSQMGN
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHCHHHCCC
NNGYCQDSEISWVHWDNLPETANALREFTRHLIQLRATQPLLRRESWRDGLEIRWFNAGG
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCC
GAQQSEQWDEGSTIGVCISRPDLQPEAGIWHDALLLFNPFEGSVPFRIPMWGEGGWVLEL
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEEE
TTADNAQQGMRITEEMDFDLAGRSIVLFRRP
ECCCCCHHCCEEHHHCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]