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The map label for this gene is rstB [H]

Identifier: 16764817

GI number: 16764817

Start: 1546405

End: 1547706

Strand: Reverse

Name: rstB [H]

Synonym: STM1471

Alternate gene names: 16764817

Gene position: 1547706-1546405 (Counterclockwise)

Preceding gene: 16764818

Following gene: 16764816

Centisome position: 31.86

GC content: 54.15

Gene sequence:

>1302_bases
ATGAAAAAGCTGTTTGTGCAGTTTTACCTTTTGCTGTTCGTCTGTTTTCTGGTCATGACCTTATTGGTCGGACTGGTGTA
CAAATTCACCGCCGAACGCGCGGGCAGGCAATCGCTCGACGATCTGATGAAAAGTTCGCTCTATCTGATGCGCAGCGAAC
TGCGGGAAATTCCGCCGCGTGAATGGGGGAAAACGTTAAAAGAGATGGACCTGAACCTCTCTTTCGATTTACGCGTTGAA
CCGTTAAATCACTATAAGCTTGATGCCGCCACCACGCAGCGACTGCGTGAAGGCGATATTGTCGCGCTTGACGATCAATA
CACCTTTATTCAGCGTATCCCGCGCAGCCATTACGTCCTGGCGGTTGGCCCGGTTCCCTATCTCTATTTCCTGCATCAAA
TGCGACTGCTGGATATCGCCTTAATGGCGCTCATTGCCTTTTCTCTCGCCTTTCCTGTGTTTATCTGGATGCGCCCGCAC
TGGCAAGAAATGCTCAGGCTCGAATCCGCCGCGCAGCGCTTTGGCGAGGGTCATTTGACTGAACGCCTGCACTTCGATAA
TGGCTCCAGTTTCGAACGTCTGGGCGTCGCGTTCAACCAGATGGCGGATAACATTAATGCCCTGATCGCCAGTAAAAAAC
AGCTGATTGACGGTATCGCCCATGAGCTTCGCACGCCGCTGGTACGTTTACGCTATCGGCTTGAAATGAGTGAAAATCTG
ACACCGCCGGAATCACAGGCGCTCAATCGCGATATTGGTCAACTGGAAGCGCTGATTGAAGAACTGCTCACCTATGCGCG
CCTTGACCGACCACAAAATGAACTGATGTTGACCGAACCCGACCTCCCCGCCTGGTTGCTCGCGCATTTGCAGGATGTGC
AGAGCGTGACCCCGGAACGTGCCGTAAACCTTGTCACATGTGTAATCGGCGATTATGGCGCGCTTGATATGCGGTTAATG
TCGCGAGTGCTGGATAACCTGCTGAATAATGCGTTGCGCTACAGTCATACCACCGTTCAGGTCAGTCTGTTACTCGACGG
CAGTCAGGCAACGTTGATAGTCGAAGATGATGGCCCCGGCATTGAAGCGGATGCGCGAGAGCGCGTATTTGAACCTTTTG
TCAGACTCGACCCCAGTAGAGACCGGGCGACAGGCGGCTGCGGGCTTGGACTCGCCATCGTACACTCTATCGCCCTGGCG
ATGGGCGGTAGCGTCGTCTGTGACGAGAGCGAACTGGGCGGCGCAAAATTTAGCTTTCGCTGGCCGGTCTGGCACGCGAT
GCCCGACATGACCGCTGCCTGA

Upstream 100 bases:

>100_bases
TGACGCAAAGCCTGCTTTTATAACCCACCAGCGGCGCTGACGGGAAATCTTTTTCGTCAGCGCGCGATAGAGCTAATGTA
TTGAGATCCGGTGGGCGTTG

Downstream 100 bases:

>100_bases
CGCATTTTGCACGCTGGTTGAGCGGCGGGAACTGTGCTATACATATAGTATGTTGTAACTAAAGTGAGTGATACCATGTC
CCGCTACGATCTCGTTGAAC

Product: sensor protein RstB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MKKLFVQFYLLLFVCFLVMTLLVGLVYKFTAERAGRQSLDDLMKSSLYLMRSELREIPPREWGKTLKEMDLNLSFDLRVE
PLNHYKLDAATTQRLREGDIVALDDQYTFIQRIPRSHYVLAVGPVPYLYFLHQMRLLDIALMALIAFSLAFPVFIWMRPH
WQEMLRLESAAQRFGEGHLTERLHFDNGSSFERLGVAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSENL
TPPESQALNRDIGQLEALIEELLTYARLDRPQNELMLTEPDLPAWLLAHLQDVQSVTPERAVNLVTCVIGDYGALDMRLM
SRVLDNLLNNALRYSHTTVQVSLLLDGSQATLIVEDDGPGIEADARERVFEPFVRLDPSRDRATGGCGLGLAIVHSIALA
MGGSVVCDESELGGAKFSFRWPVWHAMPDMTAA

Sequences:

>Translated_433_residues
MKKLFVQFYLLLFVCFLVMTLLVGLVYKFTAERAGRQSLDDLMKSSLYLMRSELREIPPREWGKTLKEMDLNLSFDLRVE
PLNHYKLDAATTQRLREGDIVALDDQYTFIQRIPRSHYVLAVGPVPYLYFLHQMRLLDIALMALIAFSLAFPVFIWMRPH
WQEMLRLESAAQRFGEGHLTERLHFDNGSSFERLGVAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSENL
TPPESQALNRDIGQLEALIEELLTYARLDRPQNELMLTEPDLPAWLLAHLQDVQSVTPERAVNLVTCVIGDYGALDMRLM
SRVLDNLLNNALRYSHTTVQVSLLLDGSQATLIVEDDGPGIEADARERVFEPFVRLDPSRDRATGGCGLGLAIVHSIALA
MGGSVVCDESELGGAKFSFRWPVWHAMPDMTAA
>Mature_433_residues
MKKLFVQFYLLLFVCFLVMTLLVGLVYKFTAERAGRQSLDDLMKSSLYLMRSELREIPPREWGKTLKEMDLNLSFDLRVE
PLNHYKLDAATTQRLREGDIVALDDQYTFIQRIPRSHYVLAVGPVPYLYFLHQMRLLDIALMALIAFSLAFPVFIWMRPH
WQEMLRLESAAQRFGEGHLTERLHFDNGSSFERLGVAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSENL
TPPESQALNRDIGQLEALIEELLTYARLDRPQNELMLTEPDLPAWLLAHLQDVQSVTPERAVNLVTCVIGDYGALDMRLM
SRVLDNLLNNALRYSHTTVQVSLLLDGSQATLIVEDDGPGIEADARERVFEPFVRLDPSRDRATGGCGLGLAIVHSIALA
MGGSVVCDESELGGAKFSFRWPVWHAMPDMTAA

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=433, Percent_Identity=82.2170900692841, Blast_Score=745, Evalue=0.0,
Organism=Escherichia coli, GI1790346, Length=273, Percent_Identity=32.6007326007326, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789808, Length=274, Percent_Identity=31.021897810219, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1788393, Length=271, Percent_Identity=29.520295202952, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786783, Length=248, Percent_Identity=32.258064516129, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1786600, Length=230, Percent_Identity=29.5652173913043, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI145693157, Length=256, Percent_Identity=27.34375, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790861, Length=216, Percent_Identity=31.4814814814815, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789403, Length=236, Percent_Identity=29.6610169491525, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI87082128, Length=269, Percent_Identity=30.1115241635688, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081816, Length=224, Percent_Identity=28.125, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790551, Length=234, Percent_Identity=29.9145299145299, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1788279, Length=292, Percent_Identity=27.3972602739726, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789149, Length=228, Percent_Identity=27.6315789473684, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787374, Length=243, Percent_Identity=25.9259259259259, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790436, Length=221, Percent_Identity=27.6018099547511, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786912, Length=220, Percent_Identity=27.2727272727273, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1788549, Length=225, Percent_Identity=25.3333333333333, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49176; Mature: 49176

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLFVQFYLLLFVCFLVMTLLVGLVYKFTAERAGRQSLDDLMKSSLYLMRSELREIPPR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCH
EWGKTLKEMDLNLSFDLRVEPLNHYKLDAATTQRLREGDIVALDDQYTFIQRIPRSHYVL
HHHHHHHHHCCCCEEEEEECCCCCEEECHHHHHHHCCCCEEEECCCHHHHHHCCCCCEEE
AVGPVPYLYFLHQMRLLDIALMALIAFSLAFPVFIWMRPHWQEMLRLESAAQRFGEGHLT
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCH
ERLHFDNGSSFERLGVAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSENL
HEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TPPESQALNRDIGQLEALIEELLTYARLDRPQNELMLTEPDLPAWLLAHLQDVQSVTPER
CCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHCCHHH
AVNLVTCVIGDYGALDMRLMSRVLDNLLNNALRYSHTTVQVSLLLDGSQATLIVEDDGPG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEEECCCCC
IEADARERVFEPFVRLDPSRDRATGGCGLGLAIVHSIALAMGGSVVCDESELGGAKFSFR
CCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEE
WPVWHAMPDMTAA
CCHHHCCCCCCCC
>Mature Secondary Structure
MKKLFVQFYLLLFVCFLVMTLLVGLVYKFTAERAGRQSLDDLMKSSLYLMRSELREIPPR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCH
EWGKTLKEMDLNLSFDLRVEPLNHYKLDAATTQRLREGDIVALDDQYTFIQRIPRSHYVL
HHHHHHHHHCCCCEEEEEECCCCCEEECHHHHHHHCCCCEEEECCCHHHHHHCCCCCEEE
AVGPVPYLYFLHQMRLLDIALMALIAFSLAFPVFIWMRPHWQEMLRLESAAQRFGEGHLT
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCH
ERLHFDNGSSFERLGVAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSENL
HEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TPPESQALNRDIGQLEALIEELLTYARLDRPQNELMLTEPDLPAWLLAHLQDVQSVTPER
CCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHCCHHH
AVNLVTCVIGDYGALDMRLMSRVLDNLLNNALRYSHTTVQVSLLLDGSQATLIVEDDGPG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEEECCCCC
IEADARERVFEPFVRLDPSRDRATGGCGLGLAIVHSIALAMGGSVVCDESELGGAKFSFR
CCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEE
WPVWHAMPDMTAA
CCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]