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The map label for this gene is manA

Identifier: 16764813

GI number: 16764813

Start: 1540812

End: 1541987

Strand: Reverse

Name: manA

Synonym: STM1467

Alternate gene names: 16764813

Gene position: 1541987-1540812 (Counterclockwise)

Preceding gene: 16764816

Following gene: 16764812

Centisome position: 31.74

GC content: 54.34

Gene sequence:

>1176_bases
ATGCAAAAACTCATTAACTCAGTGCAAAACTATGCCTGGGGAAGTAAAACTGCGTTAACGGAACTTTATGGCATCGCCAA
TCCGCAGCAGCAGCCAATGGCTGAACTCTGGATGGGCGCGCATCCCAAAAGCAGCTCGCGAATCACCACCGCCAACGGCG
AAACCGTCTCCCTGCGTGACGCCATCGAAAAGAATAAAACCGCCATGCTGGGCGAAGCGGTAGCCAACCGTTTCGGCGAA
CTGCCGTTTCTGTTTAAAGTACTGTGCGCCGCACAACCGCTCTCTATTCAGGTGCACCCGAATAAACGCAACTCCGAAAT
CGGTTTCGCGAAAGAAAATGCGGCGGGTATCCCCATGGATGCCGCAGAGCGGAACTATAAAGATCCTAACCATAAACCAG
AGCTGGTTTTTGCCCTGACGCCTTTCCTGGCGATGAACGCGTTCCGCGAATTTTCTGACATTGTCTCTTTACTGCAACCT
GTCGCCGGCGCGCATTCCGCTATCGCCCACTTTTTGCAGGTGCCGAATGCTGAACGTCTGAGCCAGCTTTTCGCCAGCCT
GTTGAATATGCAAGGCGAAGAAAAATCCCGCGCGTTAGCCGTACTCAAAGCGGCGCTTAACAGCCAGCAAGGCGAACCGT
GGCAAACGATCCGCGTGATTTCAGAGTATTATCCTGACGACAGCGGGCTTTTCTCTCCTTTGTTGCTGAATGTGGTCAAA
CTGAATCCCGGCGAGGCGATGTTCCTGTTTGCTGAAACGCCTCATGCTTATCTGCAGGGCGTTGCGCTGGAAGTCATGGC
GAACTCCGATAACGTTCTGCGCGCTGGCCTTACGCCAAAATATATCGACATCCCTGAGCTGGTCGCGAACGTGAAGTTCG
AACCTAAGCCTGCCGGCGAGTTGCTGACTGCCCCGGTGAAAAGCGGCGCGGAGCTGGACTTCCCAATTCCGGTTGACGAT
TTTGCTTTTTCACTGCACGACCTGGCGCTTCAGGAGACGAGCATCGGCCAACACAGCGCCGCGATTCTGTTCTGCGTTGA
GGGTGAGGCGGTGTTACGTAAAGATGAACAGCGTCTGGTACTGAAGCCGGGTGAATCTGCCTTTATCGGCGCGGATGAGT
CTCCGGTTAACGCCAGCGGCACGGGCCGTTTAGCGCGTGTTTATAACAAGCTGTAG

Upstream 100 bases:

>100_bases
AACTGAGACAAGTACGACTTTTTACGCCCGGAGGTTACTTCATGCGGGTTTCTTGGTTTAATACCTCCCATTGATCTCCA
CATTGAAACAGGGCTTGATA

Downstream 100 bases:

>100_bases
CAACGTACTGAATTTTTTAACAACTCTTGCTAAGCTTATAACAGACGTAAAACTCCTCCAGGCGGTTTAATCCGCCTGGT
TTCATTTTTATGGACAATTG

Product: mannose-6-phosphate isomerase

Products: NA

Alternate protein names: Phosphohexomutase; Phosphomannose isomerase; PMI

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MQKLINSVQNYAWGSKTALTELYGIANPQQQPMAELWMGAHPKSSSRITTANGETVSLRDAIEKNKTAMLGEAVANRFGE
LPFLFKVLCAAQPLSIQVHPNKRNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSDIVSLLQP
VAGAHSAIAHFLQVPNAERLSQLFASLLNMQGEEKSRALAVLKAALNSQQGEPWQTIRVISEYYPDDSGLFSPLLLNVVK
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEPKPAGELLTAPVKSGAELDFPIPVDD
FAFSLHDLALQETSIGQHSAAILFCVEGEAVLRKDEQRLVLKPGESAFIGADESPVNASGTGRLARVYNKL

Sequences:

>Translated_391_residues
MQKLINSVQNYAWGSKTALTELYGIANPQQQPMAELWMGAHPKSSSRITTANGETVSLRDAIEKNKTAMLGEAVANRFGE
LPFLFKVLCAAQPLSIQVHPNKRNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSDIVSLLQP
VAGAHSAIAHFLQVPNAERLSQLFASLLNMQGEEKSRALAVLKAALNSQQGEPWQTIRVISEYYPDDSGLFSPLLLNVVK
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEPKPAGELLTAPVKSGAELDFPIPVDD
FAFSLHDLALQETSIGQHSAAILFCVEGEAVLRKDEQRLVLKPGESAFIGADESPVNASGTGRLARVYNKL
>Mature_391_residues
MQKLINSVQNYAWGSKTALTELYGIANPQQQPMAELWMGAHPKSSSRITTANGETVSLRDAIEKNKTAMLGEAVANRFGE
LPFLFKVLCAAQPLSIQVHPNKRNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSDIVSLLQP
VAGAHSAIAHFLQVPNAERLSQLFASLLNMQGEEKSRALAVLKAALNSQQGEPWQTIRVISEYYPDDSGLFSPLLLNVVK
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEPKPAGELLTAPVKSGAELDFPIPVDD
FAFSLHDLALQETSIGQHSAAILFCVEGEAVLRKDEQRLVLKPGESAFIGADESPVNASGTGRLARVYNKL

Specific function: Involved in the conversion of glucose to GDP-L-fucose, which can be converted to L-fucose, a capsular polysaccharide

COG id: COG1482

COG function: function code G; Phosphomannose isomerase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 1 family

Homologues:

Organism=Homo sapiens, GI4505235, Length=406, Percent_Identity=34.4827586206897, Blast_Score=195, Evalue=6e-50,
Organism=Escherichia coli, GI1787899, Length=391, Percent_Identity=86.4450127877238, Blast_Score=697, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71997620, Length=403, Percent_Identity=32.258064516129, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17557650, Length=413, Percent_Identity=26.8765133171913, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6320839, Length=412, Percent_Identity=32.5242718446602, Blast_Score=185, Evalue=1e-47,
Organism=Drosophila melanogaster, GI21356061, Length=403, Percent_Identity=31.5136476426799, Blast_Score=165, Evalue=6e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MANA_SALTY (P25081)

Other databases:

- EMBL:   X57117
- EMBL:   AE006468
- PIR:   JQ1192
- RefSeq:   NP_460428.1
- PDB:   2WFP
- PDB:   3H1M
- PDB:   3H1W
- PDB:   3H1Y
- PDBsum:   2WFP
- PDBsum:   3H1M
- PDBsum:   3H1W
- PDBsum:   3H1Y
- ProteinModelPortal:   P25081
- SMR:   P25081
- PRIDE:   P25081
- GeneID:   1252985
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM1467
- NMPDR:   fig|99287.1.peg.1419
- HOGENOM:   HBG590632
- OMA:   DAPHRNY
- ProtClustDB:   PRK15131
- BioCyc:   STYP99287:STM1467-MONOMER
- BRENDA:   5.3.1.8
- GO:   GO:0005737
- InterPro:   IPR011051
- InterPro:   IPR001250
- InterPro:   IPR016305
- InterPro:   IPR018050
- InterPro:   IPR014710
- Gene3D:   G3DSA:2.60.120.10
- PIRSF:   PIRSF001480
- PRINTS:   PR00714
- TIGRFAMs:   TIGR00218

Pfam domain/function: PF01238 PMI_typeI; SSF51182 RmlC_like_cupin

EC number: =5.3.1.8

Molecular weight: Translated: 42591; Mature: 42591

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00965 PMI_I_1; PS00966 PMI_I_2

Important sites: ACT_SITE 274-274

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLINSVQNYAWGSKTALTELYGIANPQQQPMAELWMGAHPKSSSRITTANGETVSLRD
CHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCEEEECCCCEEEHHH
AIEKNKTAMLGEAVANRFGELPFLFKVLCAAQPLSIQVHPNKRNSEIGFAKENAAGIPMD
HHHHCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
AAERNYKDPNHKPELVFALTPFLAMNAFREFSDIVSLLQPVAGAHSAIAHFLQVPNAERL
HHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHH
SQLFASLLNMQGEEKSRALAVLKAALNSQQGEPWQTIRVISEYYPDDSGLFSPLLLNVVK
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHE
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEPKPAGE
ECCCCEEEEEECCCHHHHCCEEEEEEECCCCEEEECCCCCCCCHHHHHHCCCCCCCCCCC
LLTAPVKSGAELDFPIPVDDFAFSLHDLALQETSIGQHSAAILFCVEGEAVLRKDEQRLV
CEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHCCCCCEEE
LKPGESAFIGADESPVNASGTGRLARVYNKL
ECCCCCEEECCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MQKLINSVQNYAWGSKTALTELYGIANPQQQPMAELWMGAHPKSSSRITTANGETVSLRD
CHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCEEEECCCCEEEHHH
AIEKNKTAMLGEAVANRFGELPFLFKVLCAAQPLSIQVHPNKRNSEIGFAKENAAGIPMD
HHHHCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
AAERNYKDPNHKPELVFALTPFLAMNAFREFSDIVSLLQPVAGAHSAIAHFLQVPNAERL
HHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHH
SQLFASLLNMQGEEKSRALAVLKAALNSQQGEPWQTIRVISEYYPDDSGLFSPLLLNVVK
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHE
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEPKPAGE
ECCCCEEEEEECCCHHHHCCEEEEEEECCCCEEEECCCCCCCCHHHHHHCCCCCCCCCCC
LLTAPVKSGAELDFPIPVDDFAFSLHDLALQETSIGQHSAAILFCVEGEAVLRKDEQRLV
CEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHCCCCCEEE
LKPGESAFIGADESPVNASGTGRLARVYNKL
ECCCCCEEECCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1879695; 11677609