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The map label for this gene is phoQ

Identifier: 16764585

GI number: 16764585

Start: 1317226

End: 1318689

Strand: Reverse

Name: phoQ

Synonym: STM1230

Alternate gene names: 16764585

Gene position: 1318689-1317226 (Counterclockwise)

Preceding gene: 16764586

Following gene: 16764584

Centisome position: 27.15

GC content: 51.5

Gene sequence:

>1464_bases
ATGAATAAATTTGCTCGCCATTTTCTGCCGCTGTCGCTGCGGGTTCGTTTTTTGCTGGCGACAGCCGGCGTCGTGCTGGT
GCTTTCTTTGGCATATGGCATAGTGGCGCTGGTCGGCTATAGCGTAAGTTTTGATAAAACCACCTTTCGTTTGCTGCGCG
GCGAAAGCAACCTGTTTTATACCCTCGCCAAATGGGAAAATAATAAAATCAGCGTTGAGCTGCCTGAAAATCTGGACATG
CAAAGCCCGACCATGACGCTGATTTACGATGAAACGGGCAAATTATTATGGACGCAGCGCAACATTCCCTGGCTGATTAA
AAGCATTCAACCGGAATGGTTAAAAACGAACGGCTTCCATGAAATTGAAACCAACGTAGACGCCACCAGCACGCTGTTGA
GCGAAGACCATTCCGCGCAGGAAAAACTCAAAGAAGTACGTGAAGATGACGATGATGCCGAGATGACCCACTCGGTAGCG
GTAAATATTTATCCTGCCACGGCGCGGATGCCGCAGTTAACCATCGTGGTGGTCGATACCATTCCGATAGAACTAAAACG
CTCCTATATGGTGTGGAGCTGGTTCGTATACGTGCTGGCCGCCAATTTACTGTTAGTCATTCCTTTACTGTGGATCGCCG
CCTGGTGGAGCTTACGCCCTATCGAGGCGCTGGCGCGGGAAGTCCGCGAGCTTGAAGATCATCACCGCGAAATGCTCAAT
CCGGAGACGACGCGTGAGCTGACCAGCCTTGTGCGCAACCTTAATCAACTGCTCAAAAGCGAGCGTGAACGTTATAACAA
ATACCGCACGACCCTGACCGACCTGACGCACAGTTTAAAAACGCCGCTCGCGGTTTTGCAGAGTACGTTACGCTCTTTAC
GCAACGAAAAGATGAGCGTCAGCAAAGCTGAACCGGTGATGCTGGAACAGATCAGCCGGATTTCCCAGCAGATCGGCTAT
TATCTGCATCGCGCCAGTATGCGCGGTAGCGGCGTGTTGTTAAGCCGCGAACTGCATCCCGTCGCGCCGTTGTTAGATAA
CCTGATTTCTGCGCTAAATAAAGTTTATCAGCGTAAAGGGGTGAATATCAGTATGGATATTTCACCAGAAATCAGTTTTG
TCGGCGAGCAAAACGACTTTGTCGAAGTGATGGGCAACGTACTGGACAACGCTTGTAAATATTGTCTGGAGTTTGTCGAG
ATTTCGGCTCGCCAGACCGACGATCATTTGCATATTTTCGTCGAAGATGACGGCCCAGGCATTCCCCACAGCAAACGTTC
CCTGGTGTTTGATCGCGGTCAGCGCGCCGATACCCTACGACCAGGACAAGGCGTGGGGCTGGCTGTCGCGCGCGAGATTA
CGGAACAATACGCCGGGCAGATCATTGCCAGCGACAGTCTGCTCGGTGGCGCCCGTATGGAGGTCGTTTTTGGCCGACAG
CATCCCACACAGAAAGAGGAATAA

Upstream 100 bases:

>100_bases
ATGTTCTCATGGGGCGTCTGCGGAAAAAAATACAGGCCCAGTATCCGCACGATGTCATTACCACCGTACGCGGACAAGGA
TATCTTTTTGAATTGCGCTA

Downstream 100 bases:

>100_bases
ACATTTCTGTGCGTTCTTCCACGCATCTCGTTAAGCATCCGTTATAATCGGTGTCAGATACCAGCCTGCGGATACTTAAC
ATGGAATACCAATTAACTCT

Product: sensor protein PhoQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MNKFARHFLPLSLRVRFLLATAGVVLVLSLAYGIVALVGYSVSFDKTTFRLLRGESNLFYTLAKWENNKISVELPENLDM
QSPTMTLIYDETGKLLWTQRNIPWLIKSIQPEWLKTNGFHEIETNVDATSTLLSEDHSAQEKLKEVREDDDDAEMTHSVA
VNIYPATARMPQLTIVVVDTIPIELKRSYMVWSWFVYVLAANLLLVIPLLWIAAWWSLRPIEALAREVRELEDHHREMLN
PETTRELTSLVRNLNQLLKSERERYNKYRTTLTDLTHSLKTPLAVLQSTLRSLRNEKMSVSKAEPVMLEQISRISQQIGY
YLHRASMRGSGVLLSRELHPVAPLLDNLISALNKVYQRKGVNISMDISPEISFVGEQNDFVEVMGNVLDNACKYCLEFVE
ISARQTDDHLHIFVEDDGPGIPHSKRSLVFDRGQRADTLRPGQGVGLAVAREITEQYAGQIIASDSLLGGARMEVVFGRQ
HPTQKEE

Sequences:

>Translated_487_residues
MNKFARHFLPLSLRVRFLLATAGVVLVLSLAYGIVALVGYSVSFDKTTFRLLRGESNLFYTLAKWENNKISVELPENLDM
QSPTMTLIYDETGKLLWTQRNIPWLIKSIQPEWLKTNGFHEIETNVDATSTLLSEDHSAQEKLKEVREDDDDAEMTHSVA
VNIYPATARMPQLTIVVVDTIPIELKRSYMVWSWFVYVLAANLLLVIPLLWIAAWWSLRPIEALAREVRELEDHHREMLN
PETTRELTSLVRNLNQLLKSERERYNKYRTTLTDLTHSLKTPLAVLQSTLRSLRNEKMSVSKAEPVMLEQISRISQQIGY
YLHRASMRGSGVLLSRELHPVAPLLDNLISALNKVYQRKGVNISMDISPEISFVGEQNDFVEVMGNVLDNACKYCLEFVE
ISARQTDDHLHIFVEDDGPGIPHSKRSLVFDRGQRADTLRPGQGVGLAVAREITEQYAGQIIASDSLLGGARMEVVFGRQ
HPTQKEE
>Mature_487_residues
MNKFARHFLPLSLRVRFLLATAGVVLVLSLAYGIVALVGYSVSFDKTTFRLLRGESNLFYTLAKWENNKISVELPENLDM
QSPTMTLIYDETGKLLWTQRNIPWLIKSIQPEWLKTNGFHEIETNVDATSTLLSEDHSAQEKLKEVREDDDDAEMTHSVA
VNIYPATARMPQLTIVVVDTIPIELKRSYMVWSWFVYVLAANLLLVIPLLWIAAWWSLRPIEALAREVRELEDHHREMLN
PETTRELTSLVRNLNQLLKSERERYNKYRTTLTDLTHSLKTPLAVLQSTLRSLRNEKMSVSKAEPVMLEQISRISQQIGY
YLHRASMRGSGVLLSRELHPVAPLLDNLISALNKVYQRKGVNISMDISPEISFVGEQNDFVEVMGNVLDNACKYCLEFVE
ISARQTDDHLHIFVEDDGPGIPHSKRSLVFDRGQRADTLRPGQGVGLAVAREITEQYAGQIIASDSLLGGARMEVVFGRQ
HPTQKEE

Specific function: Member of the two-component regulatory system phoQ/phoP which regulates the expression of genes involved in virulence and resistance to host defense antimicrobial peptides. In presence of low periplasmic Mg(2+) concentrations, phoQ functions as a membrane

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain

Homologues:

Organism=Escherichia coli, GI1787374, Length=487, Percent_Identity=85.4209445585216, Blast_Score=874, Evalue=0.0,
Organism=Escherichia coli, GI1788393, Length=262, Percent_Identity=27.0992366412214, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790346, Length=236, Percent_Identity=25.8474576271186, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1786912, Length=275, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786783, Length=282, Percent_Identity=22.6950354609929, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790861, Length=291, Percent_Identity=27.8350515463918, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787894, Length=274, Percent_Identity=25.9124087591241, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786600, Length=267, Percent_Identity=23.9700374531835, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PHOQ_SALCH (Q57QC4)

Other databases:

- EMBL:   AE017220
- RefSeq:   YP_216168.1
- ProteinModelPortal:   Q57QC4
- PRIDE:   Q57QC4
- GeneID:   3333635
- GenomeReviews:   AE017220_GR
- KEGG:   sec:SC1181
- HOGENOM:   HBG700828
- OMA:   AHWSLRP
- ProtClustDB:   PRK10815
- BioCyc:   SENT321314:SCH_1181-MONOMER
- BRENDA:   2.7.13.3
- GO:   GO:0009405
- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR015014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- Gene3D:   G3DSA:3.30.565.10
- PRINTS:   PR00344
- SMART:   SM00387
- SMART:   SM00388

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF08918 PhoQ_Sensor; SSF55874 ATP_bd_ATPase

EC number: =2.7.13.3

Molecular weight: Translated: 55467; Mature: 55467

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50885 HAMP; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe1e6350)-; HASH(0xe4febf4)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKFARHFLPLSLRVRFLLATAGVVLVLSLAYGIVALVGYSVSFDKTTFRLLRGESNLFY
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEE
TLAKWENNKISVELPENLDMQSPTMTLIYDETGKLLWTQRNIPWLIKSIQPEWLKTNGFH
EEEECCCCEEEEECCCCCCCCCCEEEEEEECCCCEEEEECCCCHHHHCCCCCHHHCCCCE
EIETNVDATSTLLSEDHSAQEKLKEVREDDDDAEMTHSVAVNIYPATARMPQLTIVVVDT
EEECCCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHEEEEEEEECCCCCCEEEEEEEEC
IPIELKRSYMVWSWFVYVLAANLLLVIPLLWIAAWWSLRPIEALAREVRELEDHHREMLN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
PETTRELTSLVRNLNQLLKSERERYNKYRTTLTDLTHSLKTPLAVLQSTLRSLRNEKMSV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SKAEPVMLEQISRISQQIGYYLHRASMRGSGVLLSRELHPVAPLLDNLISALNKVYQRKG
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCC
VNISMDISPEISFVGEQNDFVEVMGNVLDNACKYCLEFVEISARQTDDHLHIFVEDDGPG
CEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
IPHSKRSLVFDRGQRADTLRPGQGVGLAVAREITEQYAGQIIASDSLLGGARMEVVFGRQ
CCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCEEEEEECCC
HPTQKEE
CCCCCCH
>Mature Secondary Structure
MNKFARHFLPLSLRVRFLLATAGVVLVLSLAYGIVALVGYSVSFDKTTFRLLRGESNLFY
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEE
TLAKWENNKISVELPENLDMQSPTMTLIYDETGKLLWTQRNIPWLIKSIQPEWLKTNGFH
EEEECCCCEEEEECCCCCCCCCCEEEEEEECCCCEEEEECCCCHHHHCCCCCHHHCCCCE
EIETNVDATSTLLSEDHSAQEKLKEVREDDDDAEMTHSVAVNIYPATARMPQLTIVVVDT
EEECCCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHEEEEEEEECCCCCCEEEEEEEEC
IPIELKRSYMVWSWFVYVLAANLLLVIPLLWIAAWWSLRPIEALAREVRELEDHHREMLN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
PETTRELTSLVRNLNQLLKSERERYNKYRTTLTDLTHSLKTPLAVLQSTLRSLRNEKMSV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SKAEPVMLEQISRISQQIGYYLHRASMRGSGVLLSRELHPVAPLLDNLISALNKVYQRKG
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCC
VNISMDISPEISFVGEQNDFVEVMGNVLDNACKYCLEFVEISARQTDDHLHIFVEDDGPG
CEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
IPHSKRSLVFDRGQRADTLRPGQGVGLAVAREITEQYAGQIIASDSLLGGARMEVVFGRQ
CCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCEEEEEECCC
HPTQKEE
CCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA