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The map label for this gene is hpaI [H]

Identifier: 16764464

GI number: 16764464

Start: 1193173

End: 1193964

Strand: Direct

Name: hpaI [H]

Synonym: STM1106

Alternate gene names: 16764464

Gene position: 1193173-1193964 (Clockwise)

Preceding gene: 16764463

Following gene: 16764465

Centisome position: 24.56

GC content: 60.73

Gene sequence:

>792_bases
ATGAAAAATGCTTTCAAAGACGCGTTAAAAGCGGGGCGTCCGCAAATCGGTTTGTGGCTGGGGCTTGCCAACAGTTACAG
CGCTGAACTGTTAGCGGGCGCCGGCTTCGACTGGCTACTGATCGACGGTGAACACGCGCCAAACAACGTGCAGACGGTGT
TGACCCAGTTGCAGGCGATTGCGCCTTATCCCAGCCAGCCGGTGGTGCGTCCGTCATGGAACGATCCGGTACAGATTAAG
CAACTGCTCGACGTCGGCGCGCAAACGCTGCTGATACCGATGGTGCAGAATGCCGATGAAGCGCGAAACGCCGTGGCGGC
TACGCGTTATCCGCCTGCCGGTATTCGCGGCGTGGGCAGCGCGCTGGCGCGGGCATCGCGCTGGAACCGCATTCCGGAGT
ATCTCCACCTGGCCAACGACGCCATGTGCGTACTGGTGCAGATTGAAACGCGTGAGGCGATGAGCAATCTGGCGTCAATT
CTCGACGTGGATGGCATTGACGGCGTGTTTATTGGTCCGGCGGACCTCAGCGCCGATATGGGCTTTGCCGGCAATCCGCA
GCACCCGGAAGTGCAGGCGGCGATTGAGAACGCCATCGTGCAGATACGCGCGGCGGGGAAAGCGCCGGGGATTCTGATGG
CCAATGAAGCACTGGCGAAACGTTATCTGGAACTGGGGGCGCTATTTGTCGCCGTCGGCGTTGACACTACGCTGCTGGCG
CGCGGAGCGGAGGCGCTGGCGGCGCGCTTTGGCGTAGAAAAAAAACTGTCCGGTGCGTCCGGCGTCTATTAA

Upstream 100 bases:

>100_bases
TCGTTCACCCGCCCGGTGCCGGCGCGCAAGGGCGACACCTTCCATGTCGATTACGGCAACATGGGCGCGATCAGTTGCCG
GTTTGTGTAAGGAAAAAACG

Downstream 100 bases:

>100_bases
GCCTGGCCCCGTAAGCGCAGCGCTACCGGGCAACCGTAGTACCCTACAAAATTCCCATCAGAGGAAAAAAAATGAGCGAC
ACATCATCTGCACTTCCGGA

Product: 4-hydroxyphenylacetate catabolism

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MKNAFKDALKAGRPQIGLWLGLANSYSAELLAGAGFDWLLIDGEHAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIK
QLLDVGAQTLLIPMVQNADEARNAVAATRYPPAGIRGVGSALARASRWNRIPEYLHLANDAMCVLVQIETREAMSNLASI
LDVDGIDGVFIGPADLSADMGFAGNPQHPEVQAAIENAIVQIRAAGKAPGILMANEALAKRYLELGALFVAVGVDTTLLA
RGAEALAARFGVEKKLSGASGVY

Sequences:

>Translated_263_residues
MKNAFKDALKAGRPQIGLWLGLANSYSAELLAGAGFDWLLIDGEHAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIK
QLLDVGAQTLLIPMVQNADEARNAVAATRYPPAGIRGVGSALARASRWNRIPEYLHLANDAMCVLVQIETREAMSNLASI
LDVDGIDGVFIGPADLSADMGFAGNPQHPEVQAAIENAIVQIRAAGKAPGILMANEALAKRYLELGALFVAVGVDTTLLA
RGAEALAARFGVEKKLSGASGVY
>Mature_263_residues
MKNAFKDALKAGRPQIGLWLGLANSYSAELLAGAGFDWLLIDGEHAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIK
QLLDVGAQTLLIPMVQNADEARNAVAATRYPPAGIRGVGSALARASRWNRIPEYLHLANDAMCVLVQIETREAMSNLASI
LDVDGIDGVFIGPADLSADMGFAGNPQHPEVQAAIENAIVQIRAAGKAPGILMANEALAKRYLELGALFVAVGVDTTLLA
RGAEALAARFGVEKKLSGASGVY

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788578, Length=261, Percent_Identity=56.3218390804598, Blast_Score=290, Evalue=9e-80,
Organism=Escherichia coli, GI1789514, Length=248, Percent_Identity=47.9838709677419, Blast_Score=229, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 27814; Mature: 27814

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNAFKDALKAGRPQIGLWLGLANSYSAELLAGAGFDWLLIDGEHAPNNVQTVLTQLQAI
CCHHHHHHHHCCCCCEEEEEEECCCCCHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHH
APYPSQPVVRPSWNDPVQIKQLLDVGAQTLLIPMVQNADEARNAVAATRYPPAGIRGVGS
CCCCCCCCCCCCCCCHHHHHHHHHCCHHHEEHHHHCCHHHHHHHHHHCCCCCCCHHHHHH
ALARASRWNRIPEYLHLANDAMCVLVQIETREAMSNLASILDVDGIDGVFIGPADLSADM
HHHHHHHHHCCHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCC
GFAGNPQHPEVQAAIENAIVQIRAAGKAPGILMANEALAKRYLELGALFVAVGVDTTLLA
CCCCCCCCCHHHHHHHHHHEEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHCCCHHHHH
RGAEALAARFGVEKKLSGASGVY
HHHHHHHHHHCCHHHCCCCCCCC
>Mature Secondary Structure
MKNAFKDALKAGRPQIGLWLGLANSYSAELLAGAGFDWLLIDGEHAPNNVQTVLTQLQAI
CCHHHHHHHHCCCCCEEEEEEECCCCCHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHH
APYPSQPVVRPSWNDPVQIKQLLDVGAQTLLIPMVQNADEARNAVAATRYPPAGIRGVGS
CCCCCCCCCCCCCCCHHHHHHHHHCCHHHEEHHHHCCHHHHHHHHHHCCCCCCCHHHHHH
ALARASRWNRIPEYLHLANDAMCVLVQIETREAMSNLASILDVDGIDGVFIGPADLSADM
HHHHHHHHHCCHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCC
GFAGNPQHPEVQAAIENAIVQIRAAGKAPGILMANEALAKRYLELGALFVAVGVDTTLLA
CCCCCCCCCHHHHHHHHHHEEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHCCCHHHHH
RGAEALAARFGVEKKLSGASGVY
HHHHHHHHHHCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA