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The map label for this gene is ompF [H]

Identifier: 16764359

GI number: 16764359

Start: 1089781

End: 1090872

Strand: Reverse

Name: ompF [H]

Synonym: STM0999

Alternate gene names: 16764359

Gene position: 1090872-1089781 (Counterclockwise)

Preceding gene: 16764360

Following gene: 16764358

Centisome position: 22.46

GC content: 51.1

Gene sequence:

>1092_bases
ATGATGAAGCGCAAAATCCTGGCAGCGGTGATCCCTGCCCTGCTGGCTGCTGCAACCGCAAACGCAGCAGAAATTTATAA
TAAAGATGGTAATAAGCTGGATCTGTACGGTAAAGCCGTGGGTCGTCACGTATGGACAACGACCGGCGATAGTAAAAATG
CCGACCAGACTTATGCCCAGATTGGTTTTAAAGGGGAAACGCAGATTAACACCGATCTGACCGGTTTCGGTCAGTGGGAA
TACCGTACTAAAGCAGACCGCGCTGAAGGCGAACAGCAGAACTCGAATCTGGTCCGTCTGGCTTTCGCGGGTTTGAAATA
CGCGGAAGTGGGTTCAATCGATTATGGTCGTAACTACGGTATCGTTTATGATGTTGAATCCTATACCGATATGGCCCCCT
ACTTCTCCGGCGAAACCTGGGGCGGCGCCTATACTGATAACTACATGACCAGCCGTGCTGGCGGTTTGTTGACCTACCGT
AACTCTGACTTCTTTGGTCTGGTGGACGGTCTCTCTTTCGGTATCCAGTATCAGGGTAAAAATCAGGACAACCACAGCAT
TAACTCTCAGAATGGCGATGGCGTAGGTTACACCATGGCATATGAGTTCGACGGCTTTGGCGTCACCGCAGCGTACAGCA
ACAGCAAGCGTACTAACGATCAGCAGGATCGCGATGGTAATGGCGATCGCGCAGAATCGTGGGCCGTTGGCGCGAAATAT
GATGCAAACAACGTCTACCTGGCTGCCGTATATGCTGAAACCCGCAATATGAGCATTGTTGAAAATACGGTTACCGATAC
CGTGGAAATGGCAAACAAAACGCAGAACCTGGAAGTGGTCGCTCAGTACCAGTTTGACTTCGGCCTGCGTCCGGCAATCT
CGTATGTGCAGAGTAAAGGTAAGCAGTTGAACGGCGCCGGCGGCTCGGCCGATCTGGCGAAATATATTCAGGCGGGCGCG
ACTTACTACTTCAACAAAAACATGAACGTATGGGTTGACTACCGTTTCAACCTGCTGGACGAAAACGACTACAGCTCCAG
CTACGTTGGCACCGACGATCAGGCGGCTGTCGGTATTACTTACCAGTTCTGA

Upstream 100 bases:

>100_bases
ACACCAAACTCTCATCAATATTTCTGTAAAGTTTTATTGACGGAATTTATTGACGGCAGTGGCAGGTGTCATATAAAAAA
ACCAATGAGGGTAATAAATA

Downstream 100 bases:

>100_bases
TCAGCAGACCCCTTTGTTTTATGCCTCAAAAACAGGACTTCGGTCCTGTTTTTTTATCTCTCTTAGAGAAAATGTGCGTC
TTTCAAAAACAGGCGGGCTT

Product: outer membrane protein F precursor

Products: NA

Alternate protein names: Outer membrane protein S3; Porin ompF [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MMKRKILAAVIPALLAAATANAAEIYNKDGNKLDLYGKAVGRHVWTTTGDSKNADQTYAQIGFKGETQINTDLTGFGQWE
YRTKADRAEGEQQNSNLVRLAFAGLKYAEVGSIDYGRNYGIVYDVESYTDMAPYFSGETWGGAYTDNYMTSRAGGLLTYR
NSDFFGLVDGLSFGIQYQGKNQDNHSINSQNGDGVGYTMAYEFDGFGVTAAYSNSKRTNDQQDRDGNGDRAESWAVGAKY
DANNVYLAAVYAETRNMSIVENTVTDTVEMANKTQNLEVVAQYQFDFGLRPAISYVQSKGKQLNGAGGSADLAKYIQAGA
TYYFNKNMNVWVDYRFNLLDENDYSSSYVGTDDQAAVGITYQF

Sequences:

>Translated_363_residues
MMKRKILAAVIPALLAAATANAAEIYNKDGNKLDLYGKAVGRHVWTTTGDSKNADQTYAQIGFKGETQINTDLTGFGQWE
YRTKADRAEGEQQNSNLVRLAFAGLKYAEVGSIDYGRNYGIVYDVESYTDMAPYFSGETWGGAYTDNYMTSRAGGLLTYR
NSDFFGLVDGLSFGIQYQGKNQDNHSINSQNGDGVGYTMAYEFDGFGVTAAYSNSKRTNDQQDRDGNGDRAESWAVGAKY
DANNVYLAAVYAETRNMSIVENTVTDTVEMANKTQNLEVVAQYQFDFGLRPAISYVQSKGKQLNGAGGSADLAKYIQAGA
TYYFNKNMNVWVDYRFNLLDENDYSSSYVGTDDQAAVGITYQF
>Mature_363_residues
MMKRKILAAVIPALLAAATANAAEIYNKDGNKLDLYGKAVGRHVWTTTGDSKNADQTYAQIGFKGETQINTDLTGFGQWE
YRTKADRAEGEQQNSNLVRLAFAGLKYAEVGSIDYGRNYGIVYDVESYTDMAPYFSGETWGGAYTDNYMTSRAGGLLTYR
NSDFFGLVDGLSFGIQYQGKNQDNHSINSQNGDGVGYTMAYEFDGFGVTAAYSNSKRTNDQQDRDGNGDRAESWAVGAKY
DANNVYLAAVYAETRNMSIVENTVTDTVEMANKTQNLEVVAQYQFDFGLRPAISYVQSKGKQLNGAGGSADLAKYIQAGA
TYYFNKNMNVWVDYRFNLLDENDYSSSYVGTDDQAAVGITYQF

Specific function: OmpF is a porin that forms passive diffusion pores which allow small molecular weight hydrophilic materials across the outer membrane. It is also a receptor for the bacteriophage T2 [H]

COG id: COG3203

COG function: function code M; Outer membrane protein (porin)

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Gram-negative porin family [H]

Homologues:

Organism=Escherichia coli, GI1787160, Length=369, Percent_Identity=58.5365853658537, Blast_Score=413, Evalue=1e-117,
Organism=Escherichia coli, GI1788544, Length=378, Percent_Identity=52.9100529100529, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1786436, Length=366, Percent_Identity=54.6448087431694, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1787641, Length=384, Percent_Identity=53.6458333333333, Blast_Score=363, Evalue=1e-101,

Paralogues:

None

Copy number: 1980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 17600 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12744 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001897
- InterPro:   IPR013793
- InterPro:   IPR001702 [H]

Pfam domain/function: PF00267 Porin_1 [H]

EC number: NA

Molecular weight: Translated: 40048; Mature: 40048

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00576 GRAM_NEG_PORIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKRKILAAVIPALLAAATANAAEIYNKDGNKLDLYGKAVGRHVWTTTGDSKNADQTYAQ
CCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEHHHHCCEEEEECCCCCCCCHHHHH
IGFKGETQINTDLTGFGQWEYRTKADRAEGEQQNSNLVRLAFAGLKYAEVGSIDYGRNYG
CCCCCCCEECCCCCCCCCEEEECCCHHCCCCCCCCCEEEEEECCCCEEEECCCCCCCCCC
IVYDVESYTDMAPYFSGETWGGAYTDNYMTSRAGGLLTYRNSDFFGLVDGLSFGIQYQGK
EEEEECCCCCCCCCCCCCCCCCCCCCCCHHHCCCCEEEEECCCCEEEECCCEEEEEECCC
NQDNHSINSQNGDGVGYTMAYEFDGFGVTAAYSNSKRTNDQQDRDGNGDRAESWAVGAKY
CCCCCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCEE
DANNVYLAAVYAETRNMSIVENTVTDTVEMANKTQNLEVVAQYQFDFGLRPAISYVQSKG
CCCCEEEEEEEECCCCCEEEHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHCC
KQLNGAGGSADLAKYIQAGATYYFNKNMNVWVDYRFNLLDENDYSSSYVGTDDQAAVGIT
CEECCCCCHHHHHHHHHCCEEEEEECCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEE
YQF
EEC
>Mature Secondary Structure
MMKRKILAAVIPALLAAATANAAEIYNKDGNKLDLYGKAVGRHVWTTTGDSKNADQTYAQ
CCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEHHHHCCEEEEECCCCCCCCHHHHH
IGFKGETQINTDLTGFGQWEYRTKADRAEGEQQNSNLVRLAFAGLKYAEVGSIDYGRNYG
CCCCCCCEECCCCCCCCCEEEECCCHHCCCCCCCCCEEEEEECCCCEEEECCCCCCCCCC
IVYDVESYTDMAPYFSGETWGGAYTDNYMTSRAGGLLTYRNSDFFGLVDGLSFGIQYQGK
EEEEECCCCCCCCCCCCCCCCCCCCCCCHHHCCCCEEEEECCCCEEEECCCEEEEEECCC
NQDNHSINSQNGDGVGYTMAYEFDGFGVTAAYSNSKRTNDQQDRDGNGDRAESWAVGAKY
CCCCCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCEE
DANNVYLAAVYAETRNMSIVENTVTDTVEMANKTQNLEVVAQYQFDFGLRPAISYVQSKG
CCCCEEEEEEEECCCCCEEEHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHCC
KQLNGAGGSADLAKYIQAGATYYFNKNMNVWVDYRFNLLDENDYSSSYVGTDDQAAVGIT
CEECCCCCHHHHHHHHHCCEEEEEECCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEE
YQF
EEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]