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The map label for this gene is rumB [H]

Identifier: 16764244

GI number: 16764244

Start: 953113

End: 954243

Strand: Direct

Name: rumB [H]

Synonym: STM0882

Alternate gene names: 16764244

Gene position: 953113-954243 (Clockwise)

Preceding gene: 16764243

Following gene: 16764245

Centisome position: 19.62

GC content: 55.79

Gene sequence:

>1131_bases
ATGCAGTGCGCACTCTATGACGCGGGTCGCTGCCGTTCCTGTCAATGGATTACGCAGTCGGTTAACGAACAACTTTCCGC
AAAAACAGCCGATTTACATCGTTTGCTCGCCGGGCTGCCTGTTGAACAGTGGTGCGCGCCGATTGGCGGCCCGGAACAGC
ATTTCCGCAACAAAGCGAAAATGGTCGTGAGCGGTAGTGTAGAAAAACCGCTCTTCGGGATGCTGCATCGCGACGGTACG
CCGGTCGATCTTTGCGGCTGCCCGCTGTATCCCGCCTCCTTTGACCCCGTATTCAGCGCGCTAAAACCTTTTATTGCCCG
GGCCGGACTCACGCCTTATAACGTGGCGCGAAAACGTGGCGAACTGAAATACCTGTTGCTGACGGAAAGTCAGTTCGATG
GCGGCATGATGCTACGCTTCGTGTTGCGTTCAGAAACCAAACTTACGCAACTGCGCGCGGCGTTACCGTGGCTGCGTGCG
CAACTGCCGCAGCTCAGAGTGATCACCGCAAATATCCAGCCGGTACATATGGCGATTATGGAAGGGGAGACGGAAATCTA
CCTGACGGATCAGCAGGCGCTGGCGGAACGTTTTAACGATGTGCCGCTGTGGATTCGTCCGCAAAGTTTCTTTCAGACCA
ACCCTACGGTCGCCAGCCGATTATATGCAACGGCGCGCGATTGGGTAGGGCAACTGCCGGTACGCCATATGTGGGATCTT
TTTTGCGGAGTGGGCGGATTCGGCCTGCACTGCGCGACGCCGCAGATGCAATTAACCGGTATTGAGATCGCGCCGGAGGC
GATTGCCTGCGCGAAGCAATCCGCTGCCGAGCTGGGATTGACCCGCCTTCATTTCCAGGCGTTGGATTCCACCCAATTCG
CCACCGCACAAGGCGAAACGCCCGATCTGGTGTTGGTAAATCCTCCCCGCCGCGGCATTGGCAAGCCGTTGTGCGATTAC
CTTGCACAAATGGCGCCGCGTTTTATCATTTATTCGAGCTGTAACGCGCAGACGATGGCGCAGGATATTCGTCATCTGCC
GAACTACCGTATTCAACGTGTTCAGCTTTTCGATATGTTCCCGCATACCGCGCATTATGAGGTTTTAGCGTTACTGCGCA
GGTCAATATAA

Upstream 100 bases:

>100_bases
AATTCTGTTTCTGCTGTTTATTCCCGCTCCCAGCCGTCGGTTCTTCCGGCTGCAATAATGTGTATAATCGTCGCCCCGGA
TAATATGAAGGTTTTTTGGT

Downstream 100 bases:

>100_bases
TCTATTCACAATATTTATTTTTATCCCTTAACTATTTCATAAAAATAATAAATACAATCAATTGATTATTTAATTCAATT
GGTTGTATTTATATATTACC

Product: 23S rRNA methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U747)-methyltransferase [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MQCALYDAGRCRSCQWITQSVNEQLSAKTADLHRLLAGLPVEQWCAPIGGPEQHFRNKAKMVVSGSVEKPLFGMLHRDGT
PVDLCGCPLYPASFDPVFSALKPFIARAGLTPYNVARKRGELKYLLLTESQFDGGMMLRFVLRSETKLTQLRAALPWLRA
QLPQLRVITANIQPVHMAIMEGETEIYLTDQQALAERFNDVPLWIRPQSFFQTNPTVASRLYATARDWVGQLPVRHMWDL
FCGVGGFGLHCATPQMQLTGIEIAPEAIACAKQSAAELGLTRLHFQALDSTQFATAQGETPDLVLVNPPRRGIGKPLCDY
LAQMAPRFIIYSSCNAQTMAQDIRHLPNYRIQRVQLFDMFPHTAHYEVLALLRRSI

Sequences:

>Translated_376_residues
MQCALYDAGRCRSCQWITQSVNEQLSAKTADLHRLLAGLPVEQWCAPIGGPEQHFRNKAKMVVSGSVEKPLFGMLHRDGT
PVDLCGCPLYPASFDPVFSALKPFIARAGLTPYNVARKRGELKYLLLTESQFDGGMMLRFVLRSETKLTQLRAALPWLRA
QLPQLRVITANIQPVHMAIMEGETEIYLTDQQALAERFNDVPLWIRPQSFFQTNPTVASRLYATARDWVGQLPVRHMWDL
FCGVGGFGLHCATPQMQLTGIEIAPEAIACAKQSAAELGLTRLHFQALDSTQFATAQGETPDLVLVNPPRRGIGKPLCDY
LAQMAPRFIIYSSCNAQTMAQDIRHLPNYRIQRVQLFDMFPHTAHYEVLALLRRSI
>Mature_376_residues
MQCALYDAGRCRSCQWITQSVNEQLSAKTADLHRLLAGLPVEQWCAPIGGPEQHFRNKAKMVVSGSVEKPLFGMLHRDGT
PVDLCGCPLYPASFDPVFSALKPFIARAGLTPYNVARKRGELKYLLLTESQFDGGMMLRFVLRSETKLTQLRAALPWLRA
QLPQLRVITANIQPVHMAIMEGETEIYLTDQQALAERFNDVPLWIRPQSFFQTNPTVASRLYATARDWVGQLPVRHMWDL
FCGVGGFGLHCATPQMQLTGIEIAPEAIACAKQSAAELGLTRLHFQALDSTQFATAQGETPDLVLVNPPRRGIGKPLCDY
LAQMAPRFIIYSSCNAQTMAQDIRHLPNYRIQRVQLFDMFPHTAHYEVLALLRRSI

Specific function: Catalyzes the formation of 5-methyl-uridine at position 747 (M-5-U747) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=370, Percent_Identity=24.0540540540541, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI51173878, Length=370, Percent_Identity=24.0540540540541, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787083, Length=374, Percent_Identity=87.4331550802139, Blast_Score=687, Evalue=0.0,
Organism=Escherichia coli, GI1789148, Length=384, Percent_Identity=27.34375, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790403, Length=218, Percent_Identity=26.1467889908257, Blast_Score=65, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24666579, Length=367, Percent_Identity=24.7956403269755, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011825
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.189 [H]

Molecular weight: Translated: 42181; Mature: 42181

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQCALYDAGRCRSCQWITQSVNEQLSAKTADLHRLLAGLPVEQWCAPIGGPEQHFRNKAK
CCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHCHHE
MVVSGSVEKPLFGMLHRDGTPVDLCGCPLYPASFDPVFSALKPFIARAGLTPYNVARKRG
EEEECCCCCHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCC
ELKYLLLTESQFDGGMMLRFVLRSETKLTQLRAALPWLRAQLPQLRVITANIQPVHMAIM
CEEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEE
EGETEIYLTDQQALAERFNDVPLWIRPQSFFQTNPTVASRLYATARDWVGQLPVRHMWDL
CCCEEEEEECHHHHHHHHCCCCEEECCHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
FCGVGGFGLHCATPQMQLTGIEIAPEAIACAKQSAAELGLTRLHFQALDSTQFATAQGET
HHCCCCCCEEECCCCEEEECEEECHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEECCCCC
PDLVLVNPPRRGIGKPLCDYLAQMAPRFIIYSSCNAQTMAQDIRHLPNYRIQRVQLFDMF
CCEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCCEEEEEEECCC
PHTAHYEVLALLRRSI
CCCHHHHHHHHHHHCC
>Mature Secondary Structure
MQCALYDAGRCRSCQWITQSVNEQLSAKTADLHRLLAGLPVEQWCAPIGGPEQHFRNKAK
CCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHCHHE
MVVSGSVEKPLFGMLHRDGTPVDLCGCPLYPASFDPVFSALKPFIARAGLTPYNVARKRG
EEEECCCCCHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCC
ELKYLLLTESQFDGGMMLRFVLRSETKLTQLRAALPWLRAQLPQLRVITANIQPVHMAIM
CEEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEE
EGETEIYLTDQQALAERFNDVPLWIRPQSFFQTNPTVASRLYATARDWVGQLPVRHMWDL
CCCEEEEEECHHHHHHHHCCCCEEECCHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
FCGVGGFGLHCATPQMQLTGIEIAPEAIACAKQSAAELGLTRLHFQALDSTQFATAQGET
HHCCCCCCEEECCCCEEEECEEECHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEECCCCC
PDLVLVNPPRRGIGKPLCDYLAQMAPRFIIYSSCNAQTMAQDIRHLPNYRIQRVQLFDMF
CCEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCCEEEEEEECCC
PHTAHYEVLALLRRSI
CCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA