Click here to switch to the map view.

The map label for this gene is ybjI [H]

Identifier: 16764229

GI number: 16764229

Start: 939781

End: 940596

Strand: Reverse

Name: ybjI [H]

Synonym: STM0867

Alternate gene names: 16764229

Gene position: 940596-939781 (Counterclockwise)

Preceding gene: 16764230

Following gene: 16764227

Centisome position: 19.36

GC content: 51.35

Gene sequence:

>816_bases
ATGAGTATCAAATTAATCGCCGTCGACATGGACGGCACTTTTCTGAGCGATCAAAAAACCTATAACCGTGACCGGTTTAT
GGCGCAGTACCAACAAATGAAGCGGCAAGGCATTCGCTTTGTCGTGGCCAGCGGCAACCAGTATTACCAGTTAATCTCGT
TCTTTCCGGAAATTGCGCACGAAATCGCTTTTGTCGCGGAAAACGGCGGCTGGGTGGTCAGCGAAGGCGAGGATATTTTT
AACGGCGAGCTGACAAAAGCCGACTTCCAGGCGGTTGTCGAACACCTGCTCACACGCGCCGATGTGGAGATCATCGCCTG
TGGAAAAAACAGCGCCTACACGCTGAAACGCTACGACGATGCGTTAAAAGCCGTTGCGGCAATGTATTATCATCGTCTGG
AGTTTGTGGATAATTTTAATAATATCAACGATGTCTTTTTCAAATTCGGCCTTAATATTACCGATGAACGGATACCGGAA
GTACAGGCGGCGCTGCACGACGCCATTGGCGATATCATGGTTCCCGTTCATACCGGCTATGGCAGTATTGATTTAATTAT
TCCCGGCGTGCATAAAGCCAACGGCCTGCGTCTGTTACAGCAGCGCTGGGGCATTCATGATAGTGAAGTGGTGGTATTCG
GCGACGGCGGCAACGATATTGAGATGCTGCGTCAGGCCGGGTTTAGTTTTGCCATGTCGCACGCCAGCGAAGCAGTCGCG
GCGGCGGCGAAATACCGCGCCGGATCGAACAACCAGGAAGGCGTGCTGGATATTATCGACAGCGTATTAAACAACGAGCC
GCCGTTCAACGTCTAG

Upstream 100 bases:

>100_bases
CCATGATGGTTGTGCTGGCGCTGGTTATTCTGGCGGCGCTGGTGGCGAAGGCCGTCGCCAAACCTGTATCGACTCCGCAA
CCGGTGATGGAGCATAACGC

Downstream 100 bases:

>100_bases
CGGATGCCTCTCTATGCCTGATAACAGAAAGCTTATCAGGCGTAGCCGCTCTCTCAGACTGTTTGCAAATTCCCCGTCCG
CTTATCTTTTAAGAAGACCA

Product: hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSIKLIAVDMDGTFLSDQKTYNRDRFMAQYQQMKRQGIRFVVASGNQYYQLISFFPEIAHEIAFVAENGGWVVSEGEDIF
NGELTKADFQAVVEHLLTRADVEIIACGKNSAYTLKRYDDALKAVAAMYYHRLEFVDNFNNINDVFFKFGLNITDERIPE
VQAALHDAIGDIMVPVHTGYGSIDLIIPGVHKANGLRLLQQRWGIHDSEVVVFGDGGNDIEMLRQAGFSFAMSHASEAVA
AAAKYRAGSNNQEGVLDIIDSVLNNEPPFNV

Sequences:

>Translated_271_residues
MSIKLIAVDMDGTFLSDQKTYNRDRFMAQYQQMKRQGIRFVVASGNQYYQLISFFPEIAHEIAFVAENGGWVVSEGEDIF
NGELTKADFQAVVEHLLTRADVEIIACGKNSAYTLKRYDDALKAVAAMYYHRLEFVDNFNNINDVFFKFGLNITDERIPE
VQAALHDAIGDIMVPVHTGYGSIDLIIPGVHKANGLRLLQQRWGIHDSEVVVFGDGGNDIEMLRQAGFSFAMSHASEAVA
AAAKYRAGSNNQEGVLDIIDSVLNNEPPFNV
>Mature_270_residues
SIKLIAVDMDGTFLSDQKTYNRDRFMAQYQQMKRQGIRFVVASGNQYYQLISFFPEIAHEIAFVAENGGWVVSEGEDIFN
GELTKADFQAVVEHLLTRADVEIIACGKNSAYTLKRYDDALKAVAAMYYHRLEFVDNFNNINDVFFKFGLNITDERIPEV
QAALHDAIGDIMVPVHTGYGSIDLIIPGVHKANGLRLLQQRWGIHDSEVVVFGDGGNDIEMLRQAGFSFAMSHASEAVAA
AAKYRAGSNNQEGVLDIIDSVLNNEPPFNV

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates FMN and beta-glucose 1-phosphate [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081790, Length=270, Percent_Identity=83.3333333333333, Blast_Score=472, Evalue=1e-134,
Organism=Escherichia coli, GI1787043, Length=270, Percent_Identity=53.3333333333333, Blast_Score=301, Evalue=4e-83,
Organism=Escherichia coli, GI2367265, Length=273, Percent_Identity=25.6410256410256, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 30207; Mature: 30076

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKLIAVDMDGTFLSDQKTYNRDRFMAQYQQMKRQGIRFVVASGNQYYQLISFFPEIAH
CCEEEEEEECCCCEECCCHHCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHH
EIAFVAENGGWVVSEGEDIFNGELTKADFQAVVEHLLTRADVEIIACGKNSAYTLKRYDD
HHEEEECCCCEEEECCCHHCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCEEHHHHHH
ALKAVAAMYYHRLEFVDNFNNINDVFFKFGLNITDERIPEVQAALHDAIGDIMVPVHTGY
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEECCC
GSIDLIIPGVHKANGLRLLQQRWGIHDSEVVVFGDGGNDIEMLRQAGFSFAMSHASEAVA
CCEEEEECCCCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHCCHHHHHHHHHHHHH
AAAKYRAGSNNQEGVLDIIDSVLNNEPPFNV
HHHHHCCCCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SIKLIAVDMDGTFLSDQKTYNRDRFMAQYQQMKRQGIRFVVASGNQYYQLISFFPEIAH
CEEEEEEECCCCEECCCHHCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHH
EIAFVAENGGWVVSEGEDIFNGELTKADFQAVVEHLLTRADVEIIACGKNSAYTLKRYDD
HHEEEECCCCEEEECCCHHCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCEEHHHHHH
ALKAVAAMYYHRLEFVDNFNNINDVFFKFGLNITDERIPEVQAALHDAIGDIMVPVHTGY
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEECCC
GSIDLIIPGVHKANGLRLLQQRWGIHDSEVVVFGDGGNDIEMLRQAGFSFAMSHASEAVA
CCEEEEECCCCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHCCHHHHHHHHHHHHH
AAAKYRAGSNNQEGVLDIIDSVLNNEPPFNV
HHHHHCCCCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]