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The map label for this gene is mdfA [H]

Identifier: 16764228

GI number: 16764228

Start: 938495

End: 939727

Strand: Direct

Name: mdfA [H]

Synonym: STM0866

Alternate gene names: 16764228

Gene position: 938495-939727 (Clockwise)

Preceding gene: 16764225

Following gene: 16764231

Centisome position: 19.32

GC content: 55.31

Gene sequence:

>1233_bases
ATGCAGAATCGTTTACAATCAGGCGGCAGGCTGGGACGTCAGGCGCTGCTTTTTCCTCTCTGTCTGGTGCTGTACGAGTT
TTCTACTTATATTGGCAACGACATGATCCAGCCGGGTATGCTGGCGGTGGTTGAGCAGTATCAGGCGGGCCTGGACTGGG
TTCCGACGTCCATGACCGCTTATCTGGCGGGCGGTATGTTCTTACAGTGGCTGCTTGGCCCGTTGTCTGACCGGATTGGC
CGTCGTCCCGTGATGCTGGCGGGCGTCGTGTGGTTTATTGTCACCTGCCTGGCGACGTTACTGGCGAAAAACATCGAACA
ATTTACCTTCCTGCGTTTTTTGCAAGGGATTAGCCTGTGTTTTATCGGCGCTGTAGGGTATGCCGCGATTCAGGAGTCCT
TTGAGGAGGCGGTGTGTATAAAAATTACCGCGCTGATGGCGAACGTGGCGTTAATCGCGCCGCTACTGGGACCACTGGTT
GGGGCGGCATGGGTGCATGTTCTGCCGTGGGAGGGGATGTTTATTTTATTTGCCGCACTGGCCGCTATCGCCTTTTTCGG
GCTACAGCGCGCAATGACGGAGACCGCCACGCGGCGGGGCGAAACGCTGTCATTTAAGGCGCTGGGCCGGGATTATCGGT
TGGTGATAAAAAACCGGCGGTTCGTCGCAGGGGCGTTAGCGCTGGGATTTGTCAGCCTGCCGTTGTTGGCCTGGATTGCG
CAGTCGCCGATTATCATCATTAGCGGCGAACAGCTCAGCAGCTATGAATATGGTCTGCTGCAGGTGCCCGTTTTTGGCGC
GCTCATTGCCGGTAATCTGGTGTTGGCGCGTTTAACCTCCCGACGTACGGTTCGCTCGCTGATCGTGATGGGCGGCTGGC
CTATCGTCGCGGGGCTGATCATCGCCGCGGCGGCGACGGTGGTTTCATCCCATGCTTATTTATGGATGACGGCGGGATTA
AGCGTATACGCTTTTGGCATTGGGCTGGCGAATGCCGGACTGGTGCGTCTGACGCTCTTTTCCAGCGATATGAGTAAAGG
AACGGTCTCGGCGGCGATGGGAATGCTACAAATGCTGATTTTTACCGTTGGCATTGAAGTCAGTAAACACGCCTGGTTAA
GCGGCGGCAATGGGCTATTTAGCCTGTTTAATCTGGCAAACGGTATTCTTTGGCTACTGCTGATGCTGGTCTTCTTAAAA
GATAAGCGGACGGGGAATTTGCAAACAGTCTGA

Upstream 100 bases:

>100_bases
GTAAAAATGTGCGCTATTTCCCTTGCAGCCTCATAATTCGCTATCTCAGTTCCCCGACTTTCATTACACTCTGCGCGGTT
TTTATTGGCGAAGAGATGGT

Downstream 100 bases:

>100_bases
GAGAGCGGCTACGCCTGATAAGCTTTCTGTTATCAGGCATAGAGAGGCATCCGCTAGACGTTGAACGGCGGCTCGTTGTT
TAATACGCTGTCGATAATAT

Product: multidrug translocase

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Chloramphenicol resistance pump cmr [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MQNRLQSGGRLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVVEQYQAGLDWVPTSMTAYLAGGMFLQWLLGPLSDRIG
RRPVMLAGVVWFIVTCLATLLAKNIEQFTFLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
GAAWVHVLPWEGMFILFAALAAIAFFGLQRAMTETATRRGETLSFKALGRDYRLVIKNRRFVAGALALGFVSLPLLAWIA
QSPIIIISGEQLSSYEYGLLQVPVFGALIAGNLVLARLTSRRTVRSLIVMGGWPIVAGLIIAAAATVVSSHAYLWMTAGL
SVYAFGIGLANAGLVRLTLFSSDMSKGTVSAAMGMLQMLIFTVGIEVSKHAWLSGGNGLFSLFNLANGILWLLLMLVFLK
DKRTGNLQTV

Sequences:

>Translated_410_residues
MQNRLQSGGRLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVVEQYQAGLDWVPTSMTAYLAGGMFLQWLLGPLSDRIG
RRPVMLAGVVWFIVTCLATLLAKNIEQFTFLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
GAAWVHVLPWEGMFILFAALAAIAFFGLQRAMTETATRRGETLSFKALGRDYRLVIKNRRFVAGALALGFVSLPLLAWIA
QSPIIIISGEQLSSYEYGLLQVPVFGALIAGNLVLARLTSRRTVRSLIVMGGWPIVAGLIIAAAATVVSSHAYLWMTAGL
SVYAFGIGLANAGLVRLTLFSSDMSKGTVSAAMGMLQMLIFTVGIEVSKHAWLSGGNGLFSLFNLANGILWLLLMLVFLK
DKRTGNLQTV
>Mature_410_residues
MQNRLQSGGRLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVVEQYQAGLDWVPTSMTAYLAGGMFLQWLLGPLSDRIG
RRPVMLAGVVWFIVTCLATLLAKNIEQFTFLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
GAAWVHVLPWEGMFILFAALAAIAFFGLQRAMTETATRRGETLSFKALGRDYRLVIKNRRFVAGALALGFVSLPLLAWIA
QSPIIIISGEQLSSYEYGLLQVPVFGALIAGNLVLARLTSRRTVRSLIVMGGWPIVAGLIIAAAATVVSSHAYLWMTAGL
SVYAFGIGLANAGLVRLTLFSSDMSKGTVSAAMGMLQMLIFTVGIEVSKHAWLSGGNGLFSLFNLANGILWLLLMLVFLK
DKRTGNLQTV

Specific function: Efflux pump driven by the proton motive force. Confers resistance to basic, aromatic and lipophilic drugs as well as neutral, non-aromatic, zwitterionic or non-aromatic hydrophilic antibiotics [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787065, Length=406, Percent_Identity=90.64039408867, Blast_Score=749, Evalue=0.0,
Organism=Escherichia coli, GI1790794, Length=379, Percent_Identity=41.4248021108179, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI48994889, Length=236, Percent_Identity=25.8474576271186, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788509, Length=303, Percent_Identity=22.7722772277228, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790146, Length=145, Percent_Identity=27.5862068965517, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44353; Mature: 44353

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure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HHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]