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The map label for this gene is yaeL [H]

Identifier: 16763613

GI number: 16763613

Start: 260995

End: 262347

Strand: Direct

Name: yaeL [H]

Synonym: STM0223

Alternate gene names: 16763613

Gene position: 260995-262347 (Clockwise)

Preceding gene: 16763612

Following gene: 16763614

Centisome position: 5.37

GC content: 53.07

Gene sequence:

>1353_bases
ATGCTGAGTATTCTCTGGAATCTGGCTGCATTCATCATCGCACTGGGCGTGCTGATCACTGTGCATGAATTTGGTCATTT
CTGGGTTGCCCGGCGCTGCGGTGTGCGAGTTGAGCGCTTTTCCATCGGTTTTGGCAAAGCGCTCTGGCGTCGTACCGATC
GGTACGGCACCGAGTATGTCATCGCCCTGATCCCGCTGGGCGGCTACGTCAAAATGCTGGATGAGCGCGCCGAACCGGTA
GCGCCGGAATTGCGTCGTCACGCCTTCAATAACAAAACGGTGGGCCAGCGCGCCGCAATTATTGCCGCAGGTCCGGTTGC
GAACTTCATTTTTGCTATCTTTGCTTACTGGCTGGTCTTTATCATCGGCGTCCCTGGCGTTCGTCCGGTCATTGGCGAAA
TAACGCCCAACTCGATTGCTGCGCAGGCGCAAATTGCGCCTGGTACGGAACTTAAAGCCGTTGATGGTATCGAAACCCCT
GATTGGGATGCCGTGCGTTTACAACTGGTGTCCAAAATTGGCGATCAGCAGACGACGGTGAGCGTCGCGCCGTTTGGTAG
CGACCAGCGGCAGGATAAAACGCTTGATTTACGTCACTGGGCATTTGAGCCTGATAAGCAAGATCCTGTATCTTCTTTAG
GGATTCGGCCCCGCGGGCCACAGATTGAACCGGTACTGTCAGAAGTGCAGGCGAATTCCGCAGCCAGTAAAGCGGGTTTG
CAAGCAGGCGACAGGATCGTTAAAGTCGATGGTCAGCCGTTAACGCAGTGGATGAAATTCGTGACGTTTGTACGCGATAA
TCCCGGTAAGCCGTTAGCGTTAGAGATTGAAAGGCAGGGGAGCGCCTTGTCGTTGACGTTAACGCCGGATACGAAATCGG
TTAACGGAAAGGCGGAAGGGTTTGCAGGCGTGGTGCCTAAAATTATTCCTCTGCCGGAAGAGTACAAGACTATACGCCAG
TATGGGCCTTTTAGCGCCATCCTCGAAGCCACGGATAAGACGTGGCAGTTGATGAAGCTGACGGTCAGTATGCTGGGTAA
ATTGATAACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATTTCTATCGCTCAGGGGGCTGGGATGTCAGCGGAGT
TCGGGGTGATTTATTACCTGATGTTCCTTGCGCTAATCAGCGTGAACTTAGGGATAATTAACCTGTTTCCGCTGCCCGTT
CTTGACGGGGGGCATCTGCTGTTCCTGGCGATTGAAAAGCTTAAGGGCGGGCCGGTATCCGAGCGGGTTCAAGACTTTAG
TTATCGCATTGGCTCGATTTTGCTGGTGTTGTTAATGGGGCTTGCACTTTTCAATGATTTCTCTCGGTTGTAA

Upstream 100 bases:

>100_bases
ATGGCGGCATTCTGGACCGAATTGACAGTCTGACGGCTGCCGTACCGGTCTTTGCTTGCCTGTTGTTACTGGTGTTCAGG
ACGCTCTAACGGAAGGTTTT

Downstream 100 bases:

>100_bases
GAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTCAGCAGCGCCA
CCGTATACGGTGCTGAAGGG

Product: zinc metallopeptidase RseP

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MLSILWNLAAFIIALGVLITVHEFGHFWVARRCGVRVERFSIGFGKALWRRTDRYGTEYVIALIPLGGYVKMLDERAEPV
APELRRHAFNNKTVGQRAAIIAAGPVANFIFAIFAYWLVFIIGVPGVRPVIGEITPNSIAAQAQIAPGTELKAVDGIETP
DWDAVRLQLVSKIGDQQTTVSVAPFGSDQRQDKTLDLRHWAFEPDKQDPVSSLGIRPRGPQIEPVLSEVQANSAASKAGL
QAGDRIVKVDGQPLTQWMKFVTFVRDNPGKPLALEIERQGSALSLTLTPDTKSVNGKAEGFAGVVPKIIPLPEEYKTIRQ
YGPFSAILEATDKTWQLMKLTVSMLGKLITGDVKLNNLSGPISIAQGAGMSAEFGVIYYLMFLALISVNLGIINLFPLPV
LDGGHLLFLAIEKLKGGPVSERVQDFSYRIGSILLVLLMGLALFNDFSRL

Sequences:

>Translated_450_residues
MLSILWNLAAFIIALGVLITVHEFGHFWVARRCGVRVERFSIGFGKALWRRTDRYGTEYVIALIPLGGYVKMLDERAEPV
APELRRHAFNNKTVGQRAAIIAAGPVANFIFAIFAYWLVFIIGVPGVRPVIGEITPNSIAAQAQIAPGTELKAVDGIETP
DWDAVRLQLVSKIGDQQTTVSVAPFGSDQRQDKTLDLRHWAFEPDKQDPVSSLGIRPRGPQIEPVLSEVQANSAASKAGL
QAGDRIVKVDGQPLTQWMKFVTFVRDNPGKPLALEIERQGSALSLTLTPDTKSVNGKAEGFAGVVPKIIPLPEEYKTIRQ
YGPFSAILEATDKTWQLMKLTVSMLGKLITGDVKLNNLSGPISIAQGAGMSAEFGVIYYLMFLALISVNLGIINLFPLPV
LDGGHLLFLAIEKLKGGPVSERVQDFSYRIGSILLVLLMGLALFNDFSRL
>Mature_450_residues
MLSILWNLAAFIIALGVLITVHEFGHFWVARRCGVRVERFSIGFGKALWRRTDRYGTEYVIALIPLGGYVKMLDERAEPV
APELRRHAFNNKTVGQRAAIIAAGPVANFIFAIFAYWLVFIIGVPGVRPVIGEITPNSIAAQAQIAPGTELKAVDGIETP
DWDAVRLQLVSKIGDQQTTVSVAPFGSDQRQDKTLDLRHWAFEPDKQDPVSSLGIRPRGPQIEPVLSEVQANSAASKAGL
QAGDRIVKVDGQPLTQWMKFVTFVRDNPGKPLALEIERQGSALSLTLTPDTKSVNGKAEGFAGVVPKIIPLPEEYKTIRQ
YGPFSAILEATDKTWQLMKLTVSMLGKLITGDVKLNNLSGPISIAQGAGMSAEFGVIYYLMFLALISVNLGIINLFPLPV
LDGGHLLFLAIEKLKGGPVSERVQDFSYRIGSILLVLLMGLALFNDFSRL

Specific function: Cleaves the peptide bond between 'Ala-108' and 'Cys-109' in the transmembrane region of rseA. Acts on degS-cleaved rseA to release the cytoplasmic domain of rseA. This provides the cell with sigma E (rpoE) activity through the proteolysis of rseA. In vitr

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain [H]

Homologues:

Organism=Escherichia coli, GI1786373, Length=450, Percent_Identity=88.2222222222222, Blast_Score=794, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR004387
- InterPro:   IPR008915 [H]

Pfam domain/function: PF00595 PDZ; PF02163 Peptidase_M50 [H]

EC number: NA

Molecular weight: Translated: 49185; Mature: 49185

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS50106 PDZ ; PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSILWNLAAFIIALGVLITVHEFGHFWVARRCGVRVERFSIGFGKALWRRTDRYGTEYV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHCCCCEEE
IALIPLGGYVKMLDERAEPVAPELRRHAFNNKTVGQRAAIIAAGPVANFIFAIFAYWLVF
EEEECCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
IIGVPGVRPVIGEITPNSIAAQAQIAPGTELKAVDGIETPDWDAVRLQLVSKIGDQQTTV
HHCCCCCCCHHCCCCCCCCCCEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCEEE
SVAPFGSDQRQDKTLDLRHWAFEPDKQDPVSSLGIRPRGPQIEPVLSEVQANSAASKAGL
EEECCCCCCCCCCCEEHHHCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHCHHHHHHCC
QAGDRIVKVDGQPLTQWMKFVTFVRDNPGKPLALEIERQGSALSLTLTPDTKSVNGKAEG
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCC
FAGVVPKIIPLPEEYKTIRQYGPFSAILEATDKTWQLMKLTVSMLGKLITGDVKLNNLSG
CCCCCHHHCCCCHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCC
PISIAQGAGMSAEFGVIYYLMFLALISVNLGIINLFPLPVLDGGHLLFLAIEKLKGGPVS
CEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCCEEEEEEEHHCCCCHH
ERVQDFSYRIGSILLVLLMGLALFNDFSRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSILWNLAAFIIALGVLITVHEFGHFWVARRCGVRVERFSIGFGKALWRRTDRYGTEYV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHCCCCEEE
IALIPLGGYVKMLDERAEPVAPELRRHAFNNKTVGQRAAIIAAGPVANFIFAIFAYWLVF
EEEECCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
IIGVPGVRPVIGEITPNSIAAQAQIAPGTELKAVDGIETPDWDAVRLQLVSKIGDQQTTV
HHCCCCCCCHHCCCCCCCCCCEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCEEE
SVAPFGSDQRQDKTLDLRHWAFEPDKQDPVSSLGIRPRGPQIEPVLSEVQANSAASKAGL
EEECCCCCCCCCCCEEHHHCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHCHHHHHHCC
QAGDRIVKVDGQPLTQWMKFVTFVRDNPGKPLALEIERQGSALSLTLTPDTKSVNGKAEG
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCC
FAGVVPKIIPLPEEYKTIRQYGPFSAILEATDKTWQLMKLTVSMLGKLITGDVKLNNLSG
CCCCCHHHCCCCHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCC
PISIAQGAGMSAEFGVIYYLMFLALISVNLGIINLFPLPVLDGGHLLFLAIEKLKGGPVS
CEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCCEEEEEEEHHCCCCHH
ERVQDFSYRIGSILLVLLMGLALFNDFSRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]