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The map label for this gene is ampD

Identifier: 16763536

GI number: 16763536

Start: 169354

End: 169917

Strand: Direct

Name: ampD

Synonym: STM0146

Alternate gene names: 16763536

Gene position: 169354-169917 (Clockwise)

Preceding gene: 16763531

Following gene: 16763537

Centisome position: 3.49

GC content: 55.14

Gene sequence:

>564_bases
ATGTTGCCAGATAAGGGTTGGTTGGTAGAGGCGCGACGCGTCCCTTCTCCGCATTATGATTGCCGTCCGGATGACGAAAA
ACCGTCCTTGCTGGTGGTGCATAATATTAGCCTGCCCCCCGGCGAGTTTGGCGGTCCGTGGATCGATGCATTATTCACCG
GAACGATAGATCCAGACGCCCATCCTTTTTTTGCAGAAATCGCTCATCTTCGTGTTTCGGCCCATTGTCTGATTCGTCGC
GACGGTGAAATCGTCCAGTATGTGCCTTTTGATAAGCGTGCGTGGCATGCGGGCGTGTCAAACTATCAGGGGCGGGAACG
TTGTAATGATTTCTCCATTGGTATTGAGCTGGAGGGGACGGATACGCTGGCTTATACCGATGCGCAGTATCAGCAGCTAG
CCGCCGTGACGCGCACGCTTATCGCTAGCTATCCGGCGATCGCCGACAATATGACCGGACACTGCAACATTACGCCTGAC
CGCAAAACGGACCCTGGCCCTGCCTTTGACTGGCCGCGCTTTCGCGCTTTGGTAGCCCTCTCGTCGCACAAGGAGATGAC
ATGA

Upstream 100 bases:

>100_bases
GGCGAGGCGGCATGATAAAACTCCAAATTGCTAACGAATCATAAGGTAGAAACATGCTACTCTGAAGCAGCTATAAGCAC
CACTCAAAAGGAGACTCCGC

Downstream 100 bases:

>100_bases
CGCTGTTTACGACATTACTGGTGCTGATTGTCGAGCGCCTGTTTAAGCTGGGCGAGCACTGGCAGCTCGATCATCGGCTG
GAGGCGCTATTCCGTCGGAT

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MLPDKGWLVEARRVPSPHYDCRPDDEKPSLLVVHNISLPPGEFGGPWIDALFTGTIDPDAHPFFAEIAHLRVSAHCLIRR
DGEIVQYVPFDKRAWHAGVSNYQGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRTLIASYPAIADNMTGHCNITPD
RKTDPGPAFDWPRFRALVALSSHKEMT

Sequences:

>Translated_187_residues
MLPDKGWLVEARRVPSPHYDCRPDDEKPSLLVVHNISLPPGEFGGPWIDALFTGTIDPDAHPFFAEIAHLRVSAHCLIRR
DGEIVQYVPFDKRAWHAGVSNYQGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRTLIASYPAIADNMTGHCNITPD
RKTDPGPAFDWPRFRALVALSSHKEMT
>Mature_187_residues
MLPDKGWLVEARRVPSPHYDCRPDDEKPSLLVVHNISLPPGEFGGPWIDALFTGTIDPDAHPFFAEIAHLRVSAHCLIRR
DGEIVQYVPFDKRAWHAGVSNYQGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRTLIASYPAIADNMTGHCNITPD
RKTDPGPAFDWPRFRALVALSSHKEMT

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family

Homologues:

Organism=Escherichia coli, GI1786300, Length=179, Percent_Identity=89.9441340782123, Blast_Score=339, Evalue=5e-95,
Organism=Escherichia coli, GI1787092, Length=145, Percent_Identity=36.551724137931, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMPD_SALTY (P30013)

Other databases:

- EMBL:   AJ242516
- EMBL:   AE006468
- EMBL:   L07292
- RefSeq:   NP_459151.1
- ProteinModelPortal:   P30013
- SMR:   P30013
- GeneID:   1251664
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM0146
- NMPDR:   fig|99287.1.peg.144
- HOGENOM:   HBG700567
- OMA:   VQFVSCD
- ProtClustDB:   PRK11789
- BioCyc:   STYP99287:STM0146-MONOMER
- GO:   GO:0005737
- InterPro:   IPR002502
- Gene3D:   G3DSA:3.40.80.10
- SMART:   SM00644

Pfam domain/function: PF01510 Amidase_2; SSF55846 Amidase_2

EC number: =3.5.1.28

Molecular weight: Translated: 20943; Mature: 20943

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPDKGWLVEARRVPSPHYDCRPDDEKPSLLVVHNISLPPGEFGGPWIDALFTGTIDPDA
CCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEHEEECCCCCCC
HPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSNYQGRERCNDFSIGIELEGT
CHHHHHHHHHHHEEEEEEECCCCEEEEECCCCHHHHCCCCCCCCHHHCCCEEEEEEECCC
DTLAYTDAQYQQLAAVTRTLIASYPAIADNMTGHCNITPDRKTDPGPAFDWPRFRALVAL
CEEEECCHHHHHHHHHHHHHHHHCCHHHCCCCCEEEECCCCCCCCCCCCCCHHHHEEEEE
SSHKEMT
CCCCCCC
>Mature Secondary Structure
MLPDKGWLVEARRVPSPHYDCRPDDEKPSLLVVHNISLPPGEFGGPWIDALFTGTIDPDA
CCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEHEEECCCCCCC
HPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSNYQGRERCNDFSIGIELEGT
CHHHHHHHHHHHEEEEEEECCCCEEEEECCCCHHHHCCCCCCCCHHHCCCEEEEEEECCC
DTLAYTDAQYQQLAAVTRTLIASYPAIADNMTGHCNITPDRKTDPGPAFDWPRFRALVAL
CEEEECCHHHHHHHHHHHHHHHHCCHHHCCCCCEEEECCCCCCCCCCCCCCHHHHEEEEE
SSHKEMT
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 8419294