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The map label for this gene is citE2 [H]

Identifier: 16763450

GI number: 16763450

Start: 70000

End: 70869

Strand: Direct

Name: citE2 [H]

Synonym: STM0060

Alternate gene names: 16763450

Gene position: 70000-70869 (Clockwise)

Preceding gene: 16763449

Following gene: 16763451

Centisome position: 1.44

GC content: 55.63

Gene sequence:

>870_bases
ATGAGACCACGTCGCAGTATGTTGTTTATCCCCGGCGCAAATGCGGCCATGTTGAGCACCTCGTTTGTCTACGGTGCGGA
CGCGGTGATGTTCGACTTAGAAGACGCCGTAGCGTTACGTGAGAAAGATACCGCCCGTCTTTTAGTCCATCACGCGCTTC
AACATCCGTTCTATCAGGATGTTGAAAAAGTGGTGCGCATTAATCCACTGAACACGCCGTTTGGTCTGGCCGATCTTGAA
GCCGTCGTGCGTGGTGGCGCGGATATCGTGCGTCTGCCGAAAACCGACAGCAAAAACGATGTGCTGGAGCTGGAAGCGCA
GGTCGAACGGATCGAACGTGAATGTGGACGCGAGGTCGGCAGTACCAGGCTGATGGCGGCGATCGAATCAGCGAAAGGCG
TGGTCAATGCCGTCGACATCGCCACCTCCAGCCCGCGCATGGTGGCAATTGCGCTGGCGGCATTTGATTACGTGATGGAT
ATGGGCACCAGCCGGGGCGATGGCACCGAGCTGTTCTACGCCCGCTGCGCAGTGCTGCATGCGGCGCGCGTTGCGGGTAT
TGCCGCCTATGATGTCGTTTGGTCGGATATCAATAATGAAGAAGGCTTCCTGAAGGAAGTGCAGTTGGCGAAAGGGCTTG
GTTTTAACGGTAAGTCCTTAGTGAACCCACGTCAGATTGAGCTGTTACATCAGGCTTATTCTCCGACACGTAAAGAAGTT
GAGCATGCGTATGAGGTGATTGCCGCCGCTGAAGAGGCTGAGTCTCGCGGTCTGGGCGTCGTTTCACTCAACGGCAAAAT
GATTGACGGCCCGATTATCGACCACGCGCGCAAAGTGGTGGCGCTGTCGGCTTCCGGTATTCGTGATTAA

Upstream 100 bases:

>100_bases
GCGTCGATGACAAAGGCGCGCTGGAGTGCATTTTACGCGCCCGAGTGCAGGCGGCGGCGTTACGCGCTGCAGAACAAACG
GAAATTCAATGGAGTGCGTT

Downstream 100 bases:

>100_bases
GGAAGGAACCATGAAAGAGACTGTAACCATGCTCAACCAGCAATATGTCGTACCGGAAGGATTACAGCCCTACCAGGGGG
TAACAGCGAACAGCCCGTGG

Product: citrate lyase beta chain

Products: NA

Alternate protein names: Citrase beta chain; Citrate (pro-3S)-lyase subunit beta; Citryl-CoA lyase subunit [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MRPRRSMLFIPGANAAMLSTSFVYGADAVMFDLEDAVALREKDTARLLVHHALQHPFYQDVEKVVRINPLNTPFGLADLE
AVVRGGADIVRLPKTDSKNDVLELEAQVERIERECGREVGSTRLMAAIESAKGVVNAVDIATSSPRMVAIALAAFDYVMD
MGTSRGDGTELFYARCAVLHAARVAGIAAYDVVWSDINNEEGFLKEVQLAKGLGFNGKSLVNPRQIELLHQAYSPTRKEV
EHAYEVIAAAEEAESRGLGVVSLNGKMIDGPIIDHARKVVALSASGIRD

Sequences:

>Translated_289_residues
MRPRRSMLFIPGANAAMLSTSFVYGADAVMFDLEDAVALREKDTARLLVHHALQHPFYQDVEKVVRINPLNTPFGLADLE
AVVRGGADIVRLPKTDSKNDVLELEAQVERIERECGREVGSTRLMAAIESAKGVVNAVDIATSSPRMVAIALAAFDYVMD
MGTSRGDGTELFYARCAVLHAARVAGIAAYDVVWSDINNEEGFLKEVQLAKGLGFNGKSLVNPRQIELLHQAYSPTRKEV
EHAYEVIAAAEEAESRGLGVVSLNGKMIDGPIIDHARKVVALSASGIRD
>Mature_289_residues
MRPRRSMLFIPGANAAMLSTSFVYGADAVMFDLEDAVALREKDTARLLVHHALQHPFYQDVEKVVRINPLNTPFGLADLE
AVVRGGADIVRLPKTDSKNDVLELEAQVERIERECGREVGSTRLMAAIESAKGVVNAVDIATSSPRMVAIALAAFDYVMD
MGTSRGDGTELFYARCAVLHAARVAGIAAYDVVWSDINNEEGFLKEVQLAKGLGFNGKSLVNPRQIELLHQAYSPTRKEV
EHAYEVIAAAEEAESRGLGVVSLNGKMIDGPIIDHARKVVALSASGIRD

Specific function: Represents a citryl-ACP lyase [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=295, Percent_Identity=26.4406779661017, Blast_Score=122, Evalue=3e-28,
Organism=Escherichia coli, GI87081764, Length=290, Percent_Identity=63.448275862069, Blast_Score=367, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17557458, Length=299, Percent_Identity=28.4280936454849, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR006475
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.6; =4.1.3.34 [H]

Molecular weight: Translated: 31388; Mature: 31388

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPRRSMLFIPGANAAMLSTSFVYGADAVMFDLEDAVALREKDTARLLVHHALQHPFYQD
CCCCCCEEEECCCCHHHHHHHHHCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHCCHHHH
VEKVVRINPLNTPFGLADLEAVVRGGADIVRLPKTDSKNDVLELEAQVERIERECGREVG
HHHHHEECCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHC
STRLMAAIESAKGVVNAVDIATSSPRMVAIALAAFDYVMDMGTSRGDGTELFYARCAVLH
HHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
AARVAGIAAYDVVWSDINNEEGFLKEVQLAKGLGFNGKSLVNPRQIELLHQAYSPTRKEV
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHH
EHAYEVIAAAEEAESRGLGVVSLNGKMIDGPIIDHARKVVALSASGIRD
HHHHHHHHHHHHHHHCCCEEEEECCEEECCHHHHHHHHEEEEECCCCCC
>Mature Secondary Structure
MRPRRSMLFIPGANAAMLSTSFVYGADAVMFDLEDAVALREKDTARLLVHHALQHPFYQD
CCCCCCEEEECCCCHHHHHHHHHCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHCCHHHH
VEKVVRINPLNTPFGLADLEAVVRGGADIVRLPKTDSKNDVLELEAQVERIERECGREVG
HHHHHEECCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHC
STRLMAAIESAKGVVNAVDIATSSPRMVAIALAAFDYVMDMGTSRGDGTELFYARCAVLH
HHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
AARVAGIAAYDVVWSDINNEEGFLKEVQLAKGLGFNGKSLVNPRQIELLHQAYSPTRKEV
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHH
EHAYEVIAAAEEAESRGLGVVSLNGKMIDGPIIDHARKVVALSASGIRD
HHHHHHHHHHHHHHHCCCEEEEECCEEECCHHHHHHHHEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2205500; 7830578 [H]