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The map label for this gene is leuO [H]

Identifier: 16763420

GI number: 16763420

Start: 33364

End: 34368

Strand: Direct

Name: leuO [H]

Synonym: STM0030

Alternate gene names: 16763420

Gene position: 33364-34368 (Clockwise)

Preceding gene: 39546288

Following gene: 16763422

Centisome position: 0.69

GC content: 38.81

Gene sequence:

>1005_bases
ATGCGGCCAATAAAAAATGCTAAAAAAATTGACTACAATCTGATCAAAGTGTTCGATACGGTTATTACTGAAGGAAATGC
AACCAGGGCGGCGAGGAAACTGGATGTCACGCCTGCGGCGATCTCTCAGGCTCTTCTTCGTTTACAAAATCTTTATGGCG
AAGAGTTATTTATCAGAACCCGCAAAGGATTAGTTCCGTCCAGCAAAGGTAAATCGCTTCACCAGGTATTTCGCCAGGCA
ATTGAATCTATAGAAAGCACACTGTGCGATAAAACAGATGCTCAGGAGAGTAATGAACTCATTGTTCTGGGAGGTGATAT
CACTGAAAATTACTATTTTCCGGCATTGCTGGATACTGTGTTGATGAATCGATATATTATTAAACACTATGCGATTAAAA
AAACAGGGGAATACTCACCAGCCTCCATGCTGACGCATGGCTATGCGGATGTCATCATGGGAATTCTGGAAATTAAGAAT
GAGATGATCGAAAGTTATCTTATTGATAATTTATCTGATTTTGTTTGTGTTTGTGGTGAAAAAAGTCCATTGGTTGGGCT
TGAAAAAATGTCTTTATATAATTTTTATGCTGCCAGACATGCTGTCTATCATTCAGATATGTTCTCTTCTTTCACCGCTG
ATAGCATTGATTTATTCAAGAGCAGTACGCCTTATGCGGGGCGCAGGGAAATAGGTTATTATAGTGATTCACTATTTGGC
GTTATCGATATTGTTGAAAAAAGCGATATGGTTGCGATTTTGCCAGGAAAGATTGCTACTTATTTTAGAGATGTGCGGCG
TTATAATATAAAAATACTACGTATGCCTGATGAAATGATTTTTCGTACGTTACCCGTTTATGCTTATCTGGCTACAAACA
GCACCCATTATAAAAATGTCAAAAAACTGATATCAACATTTCAGTCGACCTTTCTTTTTAGCCAGGAAAAGCAGCCTGAC
GCTTTGGTTGAAGGAAGCACATCCTTATGCGATTTGTCGGTTTAA

Upstream 100 bases:

>100_bases
ACAGATAGAGGAAATGATTTACTTATTGAAATAAGTGTGGTGCAAGGTTAATCTGCGCCTTAAATGATGGTGCCAGATTA
TACCAGCCAGGATAACCTAT

Downstream 100 bases:

>100_bases
TATCAAATTATCGTATTAAATAAATAACCATTATGACCATGGTCAGTAAAGCGAAAAATAATGCCATATATTTATACTCT
TCTTTTTTTAAAGAAGAGGG

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MRPIKNAKKIDYNLIKVFDTVITEGNATRAARKLDVTPAAISQALLRLQNLYGEELFIRTRKGLVPSSKGKSLHQVFRQA
IESIESTLCDKTDAQESNELIVLGGDITENYYFPALLDTVLMNRYIIKHYAIKKTGEYSPASMLTHGYADVIMGILEIKN
EMIESYLIDNLSDFVCVCGEKSPLVGLEKMSLYNFYAARHAVYHSDMFSSFTADSIDLFKSSTPYAGRREIGYYSDSLFG
VIDIVEKSDMVAILPGKIATYFRDVRRYNIKILRMPDEMIFRTLPVYAYLATNSTHYKNVKKLISTFQSTFLFSQEKQPD
ALVEGSTSLCDLSV

Sequences:

>Translated_334_residues
MRPIKNAKKIDYNLIKVFDTVITEGNATRAARKLDVTPAAISQALLRLQNLYGEELFIRTRKGLVPSSKGKSLHQVFRQA
IESIESTLCDKTDAQESNELIVLGGDITENYYFPALLDTVLMNRYIIKHYAIKKTGEYSPASMLTHGYADVIMGILEIKN
EMIESYLIDNLSDFVCVCGEKSPLVGLEKMSLYNFYAARHAVYHSDMFSSFTADSIDLFKSSTPYAGRREIGYYSDSLFG
VIDIVEKSDMVAILPGKIATYFRDVRRYNIKILRMPDEMIFRTLPVYAYLATNSTHYKNVKKLISTFQSTFLFSQEKQPD
ALVEGSTSLCDLSV
>Mature_334_residues
MRPIKNAKKIDYNLIKVFDTVITEGNATRAARKLDVTPAAISQALLRLQNLYGEELFIRTRKGLVPSSKGKSLHQVFRQA
IESIESTLCDKTDAQESNELIVLGGDITENYYFPALLDTVLMNRYIIKHYAIKKTGEYSPASMLTHGYADVIMGILEIKN
EMIESYLIDNLSDFVCVCGEKSPLVGLEKMSLYNFYAARHAVYHSDMFSSFTADSIDLFKSSTPYAGRREIGYYSDSLFG
VIDIVEKSDMVAILPGKIATYFRDVRRYNIKILRMPDEMIFRTLPVYAYLATNSTHYKNVKKLISTFQSTFLFSQEKQPD
ALVEGSTSLCDLSV

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081766, Length=266, Percent_Identity=25.5639097744361, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87081684, Length=288, Percent_Identity=23.6111111111111, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786820, Length=271, Percent_Identity=21.4022140221402, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 37796; Mature: 37796

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPIKNAKKIDYNLIKVFDTVITEGNATRAARKLDVTPAAISQALLRLQNLYGEELFIRT
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEH
RKGLVPSSKGKSLHQVFRQAIESIESTLCDKTDAQESNELIVLGGDITENYYFPALLDTV
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHH
LMNRYIIKHYAIKKTGEYSPASMLTHGYADVIMGILEIKNEMIESYLIDNLSDFVCVCGE
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCC
KSPLVGLEKMSLYNFYAARHAVYHSDMFSSFTADSIDLFKSSTPYAGRREIGYYSDSLFG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCHHHH
VIDIVEKSDMVAILPGKIATYFRDVRRYNIKILRMPDEMIFRTLPVYAYLATNSTHYKNV
HHHHHCCCCEEEEECHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHEEEEECCCHHHHHH
KKLISTFQSTFLFSQEKQPDALVEGSTSLCDLSV
HHHHHHHHHHHHHCCCCCCCHHHCCCCCEEECCC
>Mature Secondary Structure
MRPIKNAKKIDYNLIKVFDTVITEGNATRAARKLDVTPAAISQALLRLQNLYGEELFIRT
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEH
RKGLVPSSKGKSLHQVFRQAIESIESTLCDKTDAQESNELIVLGGDITENYYFPALLDTV
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHH
LMNRYIIKHYAIKKTGEYSPASMLTHGYADVIMGILEIKNEMIESYLIDNLSDFVCVCGE
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCC
KSPLVGLEKMSLYNFYAARHAVYHSDMFSSFTADSIDLFKSSTPYAGRREIGYYSDSLFG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCHHHH
VIDIVEKSDMVAILPGKIATYFRDVRRYNIKILRMPDEMIFRTLPVYAYLATNSTHYKNV
HHHHHCCCCEEEEECHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHEEEEECCCHHHHHH
KKLISTFQSTFLFSQEKQPDALVEGSTSLCDLSV
HHHHHHHHHHHHHCCCCCCCHHHCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 3519576 [H]