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The map label for this gene is yadB [H]

Identifier: 218930415

GI number: 218930415

Start: 3795916

End: 3796881

Strand: Direct

Name: yadB [H]

Synonym: YPO3398

Alternate gene names: 218930415

Gene position: 3795916-3796881 (Clockwise)

Preceding gene: 218930414

Following gene: 218930416

Centisome position: 81.57

GC content: 50.52

Gene sequence:

>966_bases
GTGGTTCAACAAGCGGTCATTCAACGATCAGCTAACCAACGAGCAACGAATCAACCAACAGAATATATTGGCCGTTTTGC
ACCTTCGCCATCAGGGGATTTGCATTTTGGTTCACTGATTGCCGCACTCGGGAGTTATCTGCAAGCCCGTGCTCAAGGCG
GGAAGTGGTTAGTTCGTATTGAAGATATTGATCCTCCCCGTGAAGTTCCTGGCGCGGCCTCACGTATTTTAGCGGCCTTA
GAGCATTATGGTCTCCATTGGGATGGCCCGGTTATCTATCAATCACAGCGCCATGAAGCGTATCGGGCCACGCTCAACTG
GCTGGAACAGCAGGGATTAAGCTATTACTGCACCTGCACCCGTAGCCGCATCCACCAGCTCGGCGGGTTCTACGACGGCT
ACTGCCGTGATCGTCATTTACCCGCTAGCGGCGCGGCTATCCGTTTGCGGCAGACTCAGCCGGTTTACGCTTTTTATGAT
AAATTACTCGGCGAACTGCATGCTCACCCTGCGTTGGCACAAGAAGATTTTATTATCCGTCGCCGTGACGGTCTGTTCGC
TTATAATCTGGCGGTGGTGGTGGATGATGCTTTTCAGGGCGTGACCGAAATTGTACGAGGTGCTGATTTGATTGAGCCGA
CAGTGCGCCAGATAGCGCTCTATCAGCAGTTGCAACACCCTGTCCCCGGCTATATACACTTGCCGTTGGCGCTGAATAAT
CAGGGGAATAAGCTCTCCAAGCAGAATCATGCTCCTCCCTTGCCTAATGGCGATCCACGTCCTATTTTAATCGACGCATT
AAAGTTTTTACGCCAACCGCTACCGGAATACTGGCAAGATCTTGATTTGTATTTATTATTACGTTATGCCGTTAAGCATT
GGACGTTGGTGTCAATCCCACTCCAGGGGGCAATAACCCCACAGAAAACACAACGGCATTCTCAAAGTAAGTATGGTGAG
CTATGA

Upstream 100 bases:

>100_bases
ATTCGGTTCTCAGCCATTCGCTGCTGCTCAACTATTTTCTGCTGCTCAACCACGTGCTTCCCAATTTCCCAGCGCCCAGC
TTCCCGAGACTCAACAGCCT

Downstream 100 bases:

>100_bases
TTAGCCGCTGTTTTTCTGTCGTTCTGTTATTTATTAGCCACTATCGAGGTGTACCATTTTTACCCGAGTAGCCAATTTCT
GCCGTAAGGTGCTGATCCGC

Product: glutamyl-Q tRNA(Asp) synthetase

Products: NA

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MVQQAVIQRSANQRATNQPTEYIGRFAPSPSGDLHFGSLIAALGSYLQARAQGGKWLVRIEDIDPPREVPGAASRILAAL
EHYGLHWDGPVIYQSQRHEAYRATLNWLEQQGLSYYCTCTRSRIHQLGGFYDGYCRDRHLPASGAAIRLRQTQPVYAFYD
KLLGELHAHPALAQEDFIIRRRDGLFAYNLAVVVDDAFQGVTEIVRGADLIEPTVRQIALYQQLQHPVPGYIHLPLALNN
QGNKLSKQNHAPPLPNGDPRPILIDALKFLRQPLPEYWQDLDLYLLLRYAVKHWTLVSIPLQGAITPQKTQRHSQSKYGE
L

Sequences:

>Translated_321_residues
MVQQAVIQRSANQRATNQPTEYIGRFAPSPSGDLHFGSLIAALGSYLQARAQGGKWLVRIEDIDPPREVPGAASRILAAL
EHYGLHWDGPVIYQSQRHEAYRATLNWLEQQGLSYYCTCTRSRIHQLGGFYDGYCRDRHLPASGAAIRLRQTQPVYAFYD
KLLGELHAHPALAQEDFIIRRRDGLFAYNLAVVVDDAFQGVTEIVRGADLIEPTVRQIALYQQLQHPVPGYIHLPLALNN
QGNKLSKQNHAPPLPNGDPRPILIDALKFLRQPLPEYWQDLDLYLLLRYAVKHWTLVSIPLQGAITPQKTQRHSQSKYGE
L
>Mature_321_residues
MVQQAVIQRSANQRATNQPTEYIGRFAPSPSGDLHFGSLIAALGSYLQARAQGGKWLVRIEDIDPPREVPGAASRILAAL
EHYGLHWDGPVIYQSQRHEAYRATLNWLEQQGLSYYCTCTRSRIHQLGGFYDGYCRDRHLPASGAAIRLRQTQPVYAFYD
KLLGELHAHPALAQEDFIIRRRDGLFAYNLAVVVDDAFQGVTEIVRGADLIEPTVRQIALYQQLQHPVPGYIHLPLALNN
QGNKLSKQNHAPPLPNGDPRPILIDALKFLRQPLPEYWQDLDLYLLLRYAVKHWTLVSIPLQGAITPQKTQRHSQSKYGE
L

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=249, Percent_Identity=36.9477911646586, Blast_Score=131, Evalue=7e-31,
Organism=Escherichia coli, GI226510925, Length=281, Percent_Identity=71.1743772241993, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1788743, Length=239, Percent_Identity=35.5648535564854, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71993394, Length=251, Percent_Identity=31.0756972111554, Blast_Score=111, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6324540, Length=269, Percent_Identity=32.3420074349442, Blast_Score=128, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24665321, Length=252, Percent_Identity=35.3174603174603, Blast_Score=133, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: NA

Molecular weight: Translated: 36311; Mature: 36311

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQQAVIQRSANQRATNQPTEYIGRFAPSPSGDLHFGSLIAALGSYLQARAQGGKWLVRI
CCHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
EDIDPPREVPGAASRILAALEHYGLHWDGPVIYQSQRHEAYRATLNWLEQQGLSYYCTCT
ECCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCEEEEEEH
RSRIHQLGGFYDGYCRDRHLPASGAAIRLRQTQPVYAFYDKLLGELHAHPALAQEDFIIR
HHHHHHHCCCCCCHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCEEE
RRDGLFAYNLAVVVDDAFQGVTEIVRGADLIEPTVRQIALYQQLQHPVPGYIHLPLALNN
ECCCCEEEEHHEEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECC
QGNKLSKQNHAPPLPNGDPRPILIDALKFLRQPLPEYWQDLDLYLLLRYAVKHWTLVSIP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEE
LQGAITPQKTQRHSQSKYGEL
CCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MVQQAVIQRSANQRATNQPTEYIGRFAPSPSGDLHFGSLIAALGSYLQARAQGGKWLVRI
CCHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
EDIDPPREVPGAASRILAALEHYGLHWDGPVIYQSQRHEAYRATLNWLEQQGLSYYCTCT
ECCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCEEEEEEH
RSRIHQLGGFYDGYCRDRHLPASGAAIRLRQTQPVYAFYDKLLGELHAHPALAQEDFIIR
HHHHHHHCCCCCCHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCEEE
RRDGLFAYNLAVVVDDAFQGVTEIVRGADLIEPTVRQIALYQQLQHPVPGYIHLPLALNN
ECCCCEEEEHHEEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECC
QGNKLSKQNHAPPLPNGDPRPILIDALKFLRQPLPEYWQDLDLYLLLRYAVKHWTLVSIP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEE
LQGAITPQKTQRHSQSKYGEL
CCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA