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The map label for this gene is arsB [H]

Identifier: 218930365

GI number: 218930365

Start: 3732956

End: 3734245

Strand: Direct

Name: arsB [H]

Synonym: YPO3347

Alternate gene names: 218930365

Gene position: 3732956-3734245 (Clockwise)

Preceding gene: 218930364

Following gene: 218930366

Centisome position: 80.21

GC content: 50.47

Gene sequence:

>1290_bases
ATGTTGTTGGCTGGTGCTATTTTTATCCTGACTATCGTGTTAGTTATCTGGCAACCCAAAGGGTTAGGGATTGGTTGGAG
TGCCATGCTGGGGGCAGGACTGGCATTACTCACTGGCGTGGTACATGTGAGTGATATTCCCGTGGTCTGGCAAATTGTCT
GGAACGCCACGGCGACTTTTATCGCCATCATCATCATCAGCCTGTTATTGGATGAGTCGGGGTTCTTTGAATGGGCAGCC
TTACATGTCGCACGGTGGGGTAATGGCCATGGTCGCCGCCTGTTTACTTACATCATCTTACTCGGCGCGGCAGTCGCTGC
CCTGTTTGCCAATGATGGCGCGGCGCTGATCCTCACGCCGATTGTCATTGCCATGCTATTGGCGCTGGGGTTCGGCCGTG
GCGCAACATTAGCCTTTGTGATGGCAGCCGGGTTTATTGCCGATACTGCCAGCTTGCCGCTTATCGTGTCGAACCTGGTG
AATATAGTTTCTGCTGACTTCTTTAACCTCGGGTTCACTGAATACGCGGCGGTGATGGTGCCAGTCAATCTGGTCGCCGT
TATCGCCACGCTGGCGATGCTACATCTATTTTTTCGTAAAGACATTCCGGCGGTTTATGATTTATCACTGCTGAAAGCCC
CGCAAGCGGCTATCCGCGATATGAATACGTTCAAAACCGGCTGGTTGGTCCTTGCCCTACTGCTGATCGGGTTCTTTGCT
CTGGAACCGCTCGGTGTTCCAGTCAGTTTGGTGGCCGCCGTCGGTGCGCTGATTTTGTTGGCTGTAGCGAAAAAAGGACA
TGCCATCAATACGGGAAAAGTGCTACGTGGTGCACCTTGGCAAATTGTTATTTTTTCATTAGGGATGTATCTGGTGGTTT
ATGGTCTGCGTAATGCGGGTCTGACCCACTATATTTCAGCGTTGCTAAACCGACTGGGTGAACAGGGGCTGTGGGCGTCA
ACTTTAGGGACTGGCTTCTTAACCGCGTTTCTCTCTTCCATTATGAATAATTTACCCACCGTTCTGGTAGGGGCACTTTC
TATCGAAGGCAGCACGGCAACCGGGTTAATTAAAGAGGCGATGATTTATGCCAATGTTATTGGTAGCGATCTCGGGCCGA
AAATCACCCTTATAGGTAGCCTGGCCACTTTATTGTGGCTGCATGTTTTGTCGCAAAAAAATATGACCATCACTTGGGGA
TATTATTTCCGAGTAGGGATTATCATGACCCTTCCAGTGCTATTGGTGACGTTGAGCGCGCTCGCACTACGATTGTCCGT
ATCGATGTAA

Upstream 100 bases:

>100_bases
AATGTTGTGTAACTAACCTATTTAAAATTTACCTAATTATATCTGTTTCTCCATATATGACCGAATGGAACGACACACTA
TTTAACTCAGCGGAGGCGTT

Downstream 100 bases:

>100_bases
TCACCTATTAATGTCATCATCTATTAATGTCATCACCCGTTAATATAATCGCCTGTCGATGTACTGGTCTGTTACTGTCA
TCGTCTATTTATATCCGCGC

Product: arsenical pump membrane protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: Arsenic efflux pump protein [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MLLAGAIFILTIVLVIWQPKGLGIGWSAMLGAGLALLTGVVHVSDIPVVWQIVWNATATFIAIIIISLLLDESGFFEWAA
LHVARWGNGHGRRLFTYIILLGAAVAALFANDGAALILTPIVIAMLLALGFGRGATLAFVMAAGFIADTASLPLIVSNLV
NIVSADFFNLGFTEYAAVMVPVNLVAVIATLAMLHLFFRKDIPAVYDLSLLKAPQAAIRDMNTFKTGWLVLALLLIGFFA
LEPLGVPVSLVAAVGALILLAVAKKGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAGLTHYISALLNRLGEQGLWAS
TLGTGFLTAFLSSIMNNLPTVLVGALSIEGSTATGLIKEAMIYANVIGSDLGPKITLIGSLATLLWLHVLSQKNMTITWG
YYFRVGIIMTLPVLLVTLSALALRLSVSM

Sequences:

>Translated_429_residues
MLLAGAIFILTIVLVIWQPKGLGIGWSAMLGAGLALLTGVVHVSDIPVVWQIVWNATATFIAIIIISLLLDESGFFEWAA
LHVARWGNGHGRRLFTYIILLGAAVAALFANDGAALILTPIVIAMLLALGFGRGATLAFVMAAGFIADTASLPLIVSNLV
NIVSADFFNLGFTEYAAVMVPVNLVAVIATLAMLHLFFRKDIPAVYDLSLLKAPQAAIRDMNTFKTGWLVLALLLIGFFA
LEPLGVPVSLVAAVGALILLAVAKKGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAGLTHYISALLNRLGEQGLWAS
TLGTGFLTAFLSSIMNNLPTVLVGALSIEGSTATGLIKEAMIYANVIGSDLGPKITLIGSLATLLWLHVLSQKNMTITWG
YYFRVGIIMTLPVLLVTLSALALRLSVSM
>Mature_429_residues
MLLAGAIFILTIVLVIWQPKGLGIGWSAMLGAGLALLTGVVHVSDIPVVWQIVWNATATFIAIIIISLLLDESGFFEWAA
LHVARWGNGHGRRLFTYIILLGAAVAALFANDGAALILTPIVIAMLLALGFGRGATLAFVMAAGFIADTASLPLIVSNLV
NIVSADFFNLGFTEYAAVMVPVNLVAVIATLAMLHLFFRKDIPAVYDLSLLKAPQAAIRDMNTFKTGWLVLALLLIGFFA
LEPLGVPVSLVAAVGALILLAVAKKGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAGLTHYISALLNRLGEQGLWAS
TLGTGFLTAFLSSIMNNLPTVLVGALSIEGSTATGLIKEAMIYANVIGSDLGPKITLIGSLATLLWLHVLSQKNMTITWG
YYFRVGIIMTLPVLLVTLSALALRLSVSM

Specific function: Involved in arsenical resistance. Thought to form the channel of an arsenite pump [H]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=429, Percent_Identity=83.6829836829837, Blast_Score=689, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 45547; Mature: 45547

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCC
>Mature Secondary Structure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HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9006011 [H]