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The map label for this gene is benM [H]

Identifier: 218930255

GI number: 218930255

Start: 3592389

End: 3593300

Strand: Reverse

Name: benM [H]

Synonym: YPO3228

Alternate gene names: 218930255

Gene position: 3593300-3592389 (Counterclockwise)

Preceding gene: 218930256

Following gene: 218930252

Centisome position: 77.21

GC content: 32.57

Gene sequence:

>912_bases
TTGCTTTTACTATCTAAAAGTTTAAGGTATTTCATTATTACCGCACAAAAAGAATCCATTACGCTGGCCGCAGATAAACT
GTGTATCACCGCGTCCCCTCTCAGCCGAAATATAAAGGAGCTTGAAATGAAGTTGGGTTGTGAACTTTTTACTCGAACCA
GTAAAGGGATTTTACTGACATCGAGTGGGCATTTTTTATACGATAAAATAATCCCTGTATATAACGAAATAATTGAACTT
GAAAAGGAGTTAATCAGTAATCATAAATTAAATGTTATTTCAGAAGAAAAAAGATTGGAGTTGAATATAGGGACCGATAT
TTTCCATTATGCTCATTTGTTACCGATTGCTAAGGCTCGGTTTTTTAATGAAGAAAATGTTAATTTTTCTCTAGAAGTTA
GTACTAGCCAAGATATTAGCCAGATGCTGAGAGAGAATATATATCAAGTGTTTTTCTCTCAAAGAGTTTTACCAAAGGAG
CATGGCATTATACATCGTAAATTAAGTGATGAATTTATGATGATTGCATTGCAGGATGATATGTATAGGGATGATATGAG
CCAAGAGGATATTATCTCTTCTTACCCTCTAGTCCAATATCCAAGACAAAACATCAGTAGTGTAGTTGATGAGTTAACTA
ACTATTTAATTAGTAGAAAATTGAACCCGATAAGGATCACATTACCTAACGTAAATGATCATCTATCAATAATAAATCAA
GGAGGCGCGATAGGGATATTGCCATCATCAGTTAAAAAATTAATCAATATGCATAATTATAATATTAAGCTGATTCCATT
CGTTCATAAAGGAGAGAAATTAAAAGTAAAAAGTAACGCTTACTATTTATGTGATAATAAAGATGTCTTTGAAGAAAATA
TACTCCCATTGTTGGGTGATAGTCATCTCTGA

Upstream 100 bases:

>100_bases
GTTAATAAGTTCAATATTATGATTTATATAATAATTGTCCTATCCTGCTTGCTCAGTAGAGTAAGCGTAAACCTTACATT
TTTAAATTGGGGGTGAGTAA

Downstream 100 bases:

>100_bases
ATTATAGTAAACCTCTAATTTTTAATGAATAATGATATTTTATTATGATTTATATGTTCTGCTAGGTGTTCTATGGAAAG
AGCCAGCATAAAATTATAAA

Product: putative LysR family transcriptional regulator

Products: NA

Alternate protein names: Ben and cat operon transcriptional regulator [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MLLLSKSLRYFIITAQKESITLAADKLCITASPLSRNIKELEMKLGCELFTRTSKGILLTSSGHFLYDKIIPVYNEIIEL
EKELISNHKLNVISEEKRLELNIGTDIFHYAHLLPIAKARFFNEENVNFSLEVSTSQDISQMLRENIYQVFFSQRVLPKE
HGIIHRKLSDEFMMIALQDDMYRDDMSQEDIISSYPLVQYPRQNISSVVDELTNYLISRKLNPIRITLPNVNDHLSIINQ
GGAIGILPSSVKKLINMHNYNIKLIPFVHKGEKLKVKSNAYYLCDNKDVFEENILPLLGDSHL

Sequences:

>Translated_303_residues
MLLLSKSLRYFIITAQKESITLAADKLCITASPLSRNIKELEMKLGCELFTRTSKGILLTSSGHFLYDKIIPVYNEIIEL
EKELISNHKLNVISEEKRLELNIGTDIFHYAHLLPIAKARFFNEENVNFSLEVSTSQDISQMLRENIYQVFFSQRVLPKE
HGIIHRKLSDEFMMIALQDDMYRDDMSQEDIISSYPLVQYPRQNISSVVDELTNYLISRKLNPIRITLPNVNDHLSIINQ
GGAIGILPSSVKKLINMHNYNIKLIPFVHKGEKLKVKSNAYYLCDNKDVFEENILPLLGDSHL
>Mature_303_residues
MLLLSKSLRYFIITAQKESITLAADKLCITASPLSRNIKELEMKLGCELFTRTSKGILLTSSGHFLYDKIIPVYNEIIEL
EKELISNHKLNVISEEKRLELNIGTDIFHYAHLLPIAKARFFNEENVNFSLEVSTSQDISQMLRENIYQVFFSQRVLPKE
HGIIHRKLSDEFMMIALQDDMYRDDMSQEDIISSYPLVQYPRQNISSVVDELTNYLISRKLNPIRITLPNVNDHLSIINQ
GGAIGILPSSVKKLINMHNYNIKLIPFVHKGEKLKVKSNAYYLCDNKDVFEENILPLLGDSHL

Specific function: Positive regulator of the ben and cat genes for benzoate degradation. BenM is necessary for ben gene expression but not for expression of the cat genes, which can be regulated by CatM. Binds to the inducers cis,cis-muconate and benzoate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082184, Length=83, Percent_Identity=39.7590361445783, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34885; Mature: 34885

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLLSKSLRYFIITAQKESITLAADKLCITASPLSRNIKELEMKLGCELFTRTSKGILLT
CEEEECCCEEEEEEECCCEEEEEECCEEEEECHHHCCHHHHHHHHCHHHEEECCCCEEEE
SSGHFLYDKIIPVYNEIIELEKELISNHKLNVISEEKRLELNIGTDIFHYAHLLPIAKAR
CCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCCHHHHHHHHHHHHHHH
FFNEENVNFSLEVSTSQDISQMLRENIYQVFFSQRVLPKEHGIIHRKLSDEFMMIALQDD
HCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEECC
MYRDDMSQEDIISSYPLVQYPRQNISSVVDELTNYLISRKLNPIRITLPNVNDHLSIINQ
HHHCCCCHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEC
GGAIGILPSSVKKLINMHNYNIKLIPFVHKGEKLKVKSNAYYLCDNKDVFEENILPLLGD
CCEEEECHHHHHHHHHHCCCCEEEEEEEECCCEEEEECCEEEEECCCCHHHHCCCCCCCC
SHL
CCC
>Mature Secondary Structure
MLLLSKSLRYFIITAQKESITLAADKLCITASPLSRNIKELEMKLGCELFTRTSKGILLT
CEEEECCCEEEEEEECCCEEEEEECCEEEEECHHHCCHHHHHHHHCHHHEEECCCCEEEE
SSGHFLYDKIIPVYNEIIELEKELISNHKLNVISEEKRLELNIGTDIFHYAHLLPIAKAR
CCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCCHHHHHHHHHHHHHHH
FFNEENVNFSLEVSTSQDISQMLRENIYQVFFSQRVLPKEHGIIHRKLSDEFMMIALQDD
HCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEECC
MYRDDMSQEDIISSYPLVQYPRQNISSVVDELTNYLISRKLNPIRITLPNVNDHLSIINQ
HHHCCCCHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEC
GGAIGILPSSVKKLINMHNYNIKLIPFVHKGEKLKVKSNAYYLCDNKDVFEENILPLLGD
CCEEEECHHHHHHHHHHCCCCEEEEEEEECCCEEEEECCEEEEECCCCHHHHCCCCCCCC
SHL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9573203 [H]