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The map label for this gene is purR [H]

Identifier: 218929587

GI number: 218929587

Start: 2807155

End: 2808159

Strand: Direct

Name: purR [H]

Synonym: YPO2498

Alternate gene names: 218929587

Gene position: 2807155-2808159 (Clockwise)

Preceding gene: 218929586

Following gene: 218929596

Centisome position: 60.32

GC content: 40.5

Gene sequence:

>1005_bases
GTGTCGAAAGCTTATTCAAATGCCACGATTGTGGATATTGCCCGTCGAGCTAATGTGACAAACATCACGGTTTCCCGTAC
TTTTAATCAACCTGGATTAGTTAAACAAGAAACCCGTGAAAAAATTCATGCTATCGCAAAAGAACTTAATTACGTCCCCA
ATGCTTTTGCTCAGGGATTAAAACGCAGTAGCAGCCAAATTATTGGTATTGTCACCAGCAGTATGTATAACCCTTTTTAC
TCTGAATTAATTCAAACGGTCTCCAGAATTGCACGAACACAGTGTTATCAAATTATGCTGTTTGATACCGATGGCAGTGA
AGAGGCAGAAATGGAGGCTATTCAGGCGCTATTTGGCTATAAAGCCTGTGGAATTCTTTTGTCACCGGTTCGCGATGATA
AAAACTACCAACCGGCCTATCTCGATTTAGCCGAAACCTATCGCGTTCCATTGATTCTTATTGATAGAGATATCTATAAT
CGGCAACTGAGCGGAGTATTTCTTAATAACAAAGAGATCGGGTTACTGGCGGGGAAATACCTGTCTGAGCAGCCAGAGAG
GAAAATGCTAATCATTGGTGGGCCCGCAGAATCAGAGATAACACGGGTACGTATTGAAGGGATTATCAATGCGTTAGAAA
ATAAAAAAAATGACGTGCATATTATTAATGGTGATTACGATTTTATTTCACAGGAAGCTGCCGTTAGAGATTATCTCTCT
GTCCCTCAGAATCAACCTGATTATATTATTGGCTTAAATGGAATTTTGACACTTGGCGCTATTGCAATTTGTCATGAACT
GAAAATTTATAATAATATAAAATTCTTCTCGATTGATGAACCACCTAAAGCAGCAGATTATGGCCTTCATATTGCCGGTG
TTTACCACGATACCCAGATGCTGGGGGAGATTGCTGCAGAACTCCTTTTTAATGCCATTAAAAAACCCCACAACGATCAG
GCAGTAAGACGCGAATTCTTCACCGGATCTCTGCTCACCCACTAA

Upstream 100 bases:

>100_bases
TTGTCATTATTTCCGACAAAGACACTCACTTATATCTGGCATACTTTAAGCGGCTTCCCTACAATAAGTATTCAGTTGAC
GAATAAAGCAAGGAATAAAG

Downstream 100 bases:

>100_bases
AGCGGCCTACGAAAATATGGGGCACCCATGTTCAACCCGCCGTAATGGCGGCCATTATCGCGCCGCCATTACCCTCACTG
CATCAACTTAATTACTGTAT

Product: putative LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSKAYSNATIVDIARRANVTNITVSRTFNQPGLVKQETREKIHAIAKELNYVPNAFAQGLKRSSSQIIGIVTSSMYNPFY
SELIQTVSRIARTQCYQIMLFDTDGSEEAEMEAIQALFGYKACGILLSPVRDDKNYQPAYLDLAETYRVPLILIDRDIYN
RQLSGVFLNNKEIGLLAGKYLSEQPERKMLIIGGPAESEITRVRIEGIINALENKKNDVHIINGDYDFISQEAAVRDYLS
VPQNQPDYIIGLNGILTLGAIAICHELKIYNNIKFFSIDEPPKAADYGLHIAGVYHDTQMLGEIAAELLFNAIKKPHNDQ
AVRREFFTGSLLTH

Sequences:

>Translated_334_residues
MSKAYSNATIVDIARRANVTNITVSRTFNQPGLVKQETREKIHAIAKELNYVPNAFAQGLKRSSSQIIGIVTSSMYNPFY
SELIQTVSRIARTQCYQIMLFDTDGSEEAEMEAIQALFGYKACGILLSPVRDDKNYQPAYLDLAETYRVPLILIDRDIYN
RQLSGVFLNNKEIGLLAGKYLSEQPERKMLIIGGPAESEITRVRIEGIINALENKKNDVHIINGDYDFISQEAAVRDYLS
VPQNQPDYIIGLNGILTLGAIAICHELKIYNNIKFFSIDEPPKAADYGLHIAGVYHDTQMLGEIAAELLFNAIKKPHNDQ
AVRREFFTGSLLTH
>Mature_333_residues
SKAYSNATIVDIARRANVTNITVSRTFNQPGLVKQETREKIHAIAKELNYVPNAFAQGLKRSSSQIIGIVTSSMYNPFYS
ELIQTVSRIARTQCYQIMLFDTDGSEEAEMEAIQALFGYKACGILLSPVRDDKNYQPAYLDLAETYRVPLILIDRDIYNR
QLSGVFLNNKEIGLLAGKYLSEQPERKMLIIGGPAESEITRVRIEGIINALENKKNDVHIINGDYDFISQEAAVRDYLSV
PQNQPDYIIGLNGILTLGAIAICHELKIYNNIKFFSIDEPPKAADYGLHIAGVYHDTQMLGEIAAELLFNAIKKPHNDQA
VRREFFTGSLLTH

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=314, Percent_Identity=27.7070063694268, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787948, Length=313, Percent_Identity=27.1565495207668, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1790369, Length=294, Percent_Identity=25.5102040816327, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789068, Length=266, Percent_Identity=26.3157894736842, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786268, Length=216, Percent_Identity=26.3888888888889, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1786540, Length=312, Percent_Identity=25, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1788474, Length=240, Percent_Identity=28.3333333333333, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI48994940, Length=141, Percent_Identity=28.3687943262411, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789202, Length=242, Percent_Identity=24.3801652892562, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790715, Length=330, Percent_Identity=23.9393939393939, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787580, Length=332, Percent_Identity=25, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787906, Length=199, Percent_Identity=23.6180904522613, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37496; Mature: 37365

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAYSNATIVDIARRANVTNITVSRTFNQPGLVKQETREKIHAIAKELNYVPNAFAQGL
CCCCCCCCHHEEHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
KRSSSQIIGIVTSSMYNPFYSELIQTVSRIARTQCYQIMLFDTDGSEEAEMEAIQALFGY
HCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHCEEEEEEEECCCCCHHHHHHHHHHHHH
KACGILLSPVRDDKNYQPAYLDLAETYRVPLILIDRDIYNRQLSGVFLNNKEIGLLAGKY
HHHHHEEECCCCCCCCCCCEEEHHHHCCCCEEEEECHHHCCEECEEEECCCCEEEEECHH
LSEQPERKMLIIGGPAESEITRVRIEGIINALENKKNDVHIINGDYDFISQEAAVRDYLS
HCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHH
VPQNQPDYIIGLNGILTLGAIAICHELKIYNNIKFFSIDEPPKAADYGLHIAGVYHDTQM
CCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEEEEHHHHH
LGEIAAELLFNAIKKPHNDQAVRREFFTGSLLTH
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHCCC
>Mature Secondary Structure 
SKAYSNATIVDIARRANVTNITVSRTFNQPGLVKQETREKIHAIAKELNYVPNAFAQGL
CCCCCCCHHEEHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
KRSSSQIIGIVTSSMYNPFYSELIQTVSRIARTQCYQIMLFDTDGSEEAEMEAIQALFGY
HCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHCEEEEEEEECCCCCHHHHHHHHHHHHH
KACGILLSPVRDDKNYQPAYLDLAETYRVPLILIDRDIYNRQLSGVFLNNKEIGLLAGKY
HHHHHEEECCCCCCCCCCCEEEHHHHCCCCEEEEECHHHCCEECEEEECCCCEEEEECHH
LSEQPERKMLIIGGPAESEITRVRIEGIINALENKKNDVHIINGDYDFISQEAAVRDYLS
HCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHH
VPQNQPDYIIGLNGILTLGAIAICHELKIYNNIKFFSIDEPPKAADYGLHIAGVYHDTQM
CCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEEEEHHHHH
LGEIAAELLFNAIKKPHNDQAVRREFFTGSLLTH
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA