Click here to switch to the map view.

The map label for this gene is pheT

Identifier: 218929516

GI number: 218929516

Start: 2728077

End: 2730464

Strand: Reverse

Name: pheT

Synonym: YPO2428

Alternate gene names: 218929516

Gene position: 2730464-2728077 (Counterclockwise)

Preceding gene: 218929517

Following gene: 218929515

Centisome position: 58.67

GC content: 48.49

Gene sequence:

>2388_bases
ATGAAATTCAGTGAACTTTGGTTACGCGAATGGGTAAATCCAGCCATTAGCAGCGATGATTTAGCTCATCAGATTACCAT
GGCCGGGCTAGAGGTTGATGGTGTAGACGCGGTTGCCGGTGAGTTTAACGGCGTTGTTGTGGGTCATGTCGTGGAGTGTG
GGCAACATCCAAATGCAGATAAGCTGCGAGTGACCAAAATTGATGTCGGCGGTGATCGCCTCCTGGACATCGTTTGTGGT
GCACCTAATTGCCGTCAAGGCCTCAAAGTTGCAGTTGCAACGGTAGGGGCGGTATTACCGGGTGACTTTAAAATTAAAGC
CGCCAAACTGCGTGGTGAGTCTTCAGAAGGGATGCTGTGCTCTTTTTCTGAGCTGGCTATTTCAGACGATCATGATGGTA
TTATTGAATTGCCCGCAGATGCTCCCATTGGTGTCGATGTGCGTGAGTATTTGCACTTGGACGACAAGACGATTGAAATC
AGCGTAACGCCAAACCGTGCTGATTGTTTAGGGATTATTGGTGTCGCTCGTGATGTAGCTGTCGTTAATCAATTGCCATT
AACTGAACCGGATATGGCTGAAGTTGTGGCATCAATTAATGACACACTTCCTATTCGTGTCGATGCACCACAAGCTTGCC
CACGTTATCTGGGCCGTGTGGTGAAGGGAATTAATGTTAAGGCTGCGACACCCCTATGGATGCGTGAAAAATTGCGCCGT
TGTGGCATTCGTTCAATTGATCCTGTTGTCGATGTGACCAACTTTGTGTTGCTGGAGCTAGGGCAGCCAATGCATGCCTT
TGATCTGGATCGTATCAGTGGCGGGGTCATCGTTCGTCTGGCAACTGACGGCGAAGAACTCACGCTATTAGATGGTACAA
AAGCCAAGTTGAACGCAGACACACTGGTTATTGCCGATCATCAGAAACCATTGGCGATGGCGGGTATCTTTGGTGGCGAA
CATTCTGGTGTCAATGAAGAAACCAGAAATGTATTGCTGGAGTCCGCGTTCTTCAATCCACTGTCTATTACTGGCCGAGC
ACGCCGCCATGGCTTACATACTGATGCCTCTCATCGTTATGAGCGTGGTGTCGACCCAGCGTTACAGCATAAAGCTATCG
AACGTGCGACACGTCTACTGATTGATATTTGTGGTGGGGAAGCCGGGCCGGTTGTGGATGTGACGACAGCATCAGAACTA
CCCAAACAAGCCACTATCACATTGCGCCGTGAGAAGCTGGATCGCCTAATTGGTCATCATATTCCATCTGAACAGGTGAG
TGATATTTTGCGTCGCTTAGGATGCAAAGTGACTGAATGTGGTTCTGATTGGCAGGCTGTAGCACCGAGCTGGCGTTTCG
ATATGGCTATCGAAGAAGACCTGGTGGAAGAAGTCGCCCGTATTTATGGCTATAACAATATTCCAGATGTCCCCGTTAGA
GCTGATTTGGTAATGACTAAACACCGTGAAGCAAGCTTGTCGCTGAAGCGGGTAAAGACAGCTTTGGTGGATCGTGGTTA
TCAGGAAGCAATTACTTACAGTTTTGTTGATCCTAAAGTACAGGCGTTACTGCATCCACAACAGGAAGCCCTCATTTTAC
CAAACCCTATTTCAGTTGATATGTCCGCAATGCGTTTATCACTGCTAACGGGTTTGCTGTCGGCAGTCGTTTATAATCAG
AACCGTCAGCAATCTCGGCTGCGATTATTCGAAAGCGGTTTACGCTTTGTTCCTGATAATACGGCAGATTTAGGCATTAG
GCAGGATGTAATGCTGGCAGGTGTTATTGCTGGTCATCGTTACGATGAGCATTGGGATCTGGCGCGTCAGCCGATCGACT
TCTATGATTTGAAAGGTGATTTGGAAGCAATTCTGGAGCTTACCGGCAAATTATCTGATGTTCAGTTCCGGGCAGAAGCG
CATTCTGCTCTGCACCCTGGGCAGAGTGCTGCAATTTATTTAGCGGGTGAACACATTGGTTTCATTGGTGTAGTTCACCC
AGAATTGGAACGTAAGCTGGATCTGAATGGTCGAACTGTCGTGTTTGAAGTGCAGTGGAATAAGCTTGCAGACCGCGCTG
TGCCTCAGGCCAGAGAGATTTCTCGCTTCCCGGCGAACCGTCGTGATATCGCTGTTGTGGTGGCTGAAAACGTTCCCGCA
GAAGATATTTTGGCCGAGTGTAAGAAAGTTGGCGCAAATCAGGTAGTTGGCGTAAACTTGTTTGATGTGTACAGTGGCAA
GGGCGTAGCAGAGGGCTATAAGAGTCTGGCTATTAGTCTGGTGTTGCAGGATACCGCCCGTACACTGGAAGAAGAGGAGA
TCGCCGCTACCGTTGCAAAATGCGTAGAGGCATTAAAACAGCGATTCCAAGCATCCTTGAGGGATTGA

Upstream 100 bases:

>100_bases
GAACGTTTAACGATGTTACGTTACGGCGTCACCGATTTGCGTGCATTCTTCGAAAACGATCTGCGTTTCCTCAAACAGTT
TAAGTAAGGCAGGATATCTC

Downstream 100 bases:

>100_bases
ACCTATGGCGCTTACTAAAGCTGAAATGTCAGAACATCTGTTTGAAAAGCTAGGGCTTAGCAAACGAGATGCTAAAGATC
TGGTTGAGTTATTCTTCGAA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS

Number of amino acids: Translated: 795; Mature: 795

Protein sequence:

>795_residues
MKFSELWLREWVNPAISSDDLAHQITMAGLEVDGVDAVAGEFNGVVVGHVVECGQHPNADKLRVTKIDVGGDRLLDIVCG
APNCRQGLKVAVATVGAVLPGDFKIKAAKLRGESSEGMLCSFSELAISDDHDGIIELPADAPIGVDVREYLHLDDKTIEI
SVTPNRADCLGIIGVARDVAVVNQLPLTEPDMAEVVASINDTLPIRVDAPQACPRYLGRVVKGINVKAATPLWMREKLRR
CGIRSIDPVVDVTNFVLLELGQPMHAFDLDRISGGVIVRLATDGEELTLLDGTKAKLNADTLVIADHQKPLAMAGIFGGE
HSGVNEETRNVLLESAFFNPLSITGRARRHGLHTDASHRYERGVDPALQHKAIERATRLLIDICGGEAGPVVDVTTASEL
PKQATITLRREKLDRLIGHHIPSEQVSDILRRLGCKVTECGSDWQAVAPSWRFDMAIEEDLVEEVARIYGYNNIPDVPVR
ADLVMTKHREASLSLKRVKTALVDRGYQEAITYSFVDPKVQALLHPQQEALILPNPISVDMSAMRLSLLTGLLSAVVYNQ
NRQQSRLRLFESGLRFVPDNTADLGIRQDVMLAGVIAGHRYDEHWDLARQPIDFYDLKGDLEAILELTGKLSDVQFRAEA
HSALHPGQSAAIYLAGEHIGFIGVVHPELERKLDLNGRTVVFEVQWNKLADRAVPQAREISRFPANRRDIAVVVAENVPA
EDILAECKKVGANQVVGVNLFDVYSGKGVAEGYKSLAISLVLQDTARTLEEEEIAATVAKCVEALKQRFQASLRD

Sequences:

>Translated_795_residues
MKFSELWLREWVNPAISSDDLAHQITMAGLEVDGVDAVAGEFNGVVVGHVVECGQHPNADKLRVTKIDVGGDRLLDIVCG
APNCRQGLKVAVATVGAVLPGDFKIKAAKLRGESSEGMLCSFSELAISDDHDGIIELPADAPIGVDVREYLHLDDKTIEI
SVTPNRADCLGIIGVARDVAVVNQLPLTEPDMAEVVASINDTLPIRVDAPQACPRYLGRVVKGINVKAATPLWMREKLRR
CGIRSIDPVVDVTNFVLLELGQPMHAFDLDRISGGVIVRLATDGEELTLLDGTKAKLNADTLVIADHQKPLAMAGIFGGE
HSGVNEETRNVLLESAFFNPLSITGRARRHGLHTDASHRYERGVDPALQHKAIERATRLLIDICGGEAGPVVDVTTASEL
PKQATITLRREKLDRLIGHHIPSEQVSDILRRLGCKVTECGSDWQAVAPSWRFDMAIEEDLVEEVARIYGYNNIPDVPVR
ADLVMTKHREASLSLKRVKTALVDRGYQEAITYSFVDPKVQALLHPQQEALILPNPISVDMSAMRLSLLTGLLSAVVYNQ
NRQQSRLRLFESGLRFVPDNTADLGIRQDVMLAGVIAGHRYDEHWDLARQPIDFYDLKGDLEAILELTGKLSDVQFRAEA
HSALHPGQSAAIYLAGEHIGFIGVVHPELERKLDLNGRTVVFEVQWNKLADRAVPQAREISRFPANRRDIAVVVAENVPA
EDILAECKKVGANQVVGVNLFDVYSGKGVAEGYKSLAISLVLQDTARTLEEEEIAATVAKCVEALKQRFQASLRD
>Mature_795_residues
MKFSELWLREWVNPAISSDDLAHQITMAGLEVDGVDAVAGEFNGVVVGHVVECGQHPNADKLRVTKIDVGGDRLLDIVCG
APNCRQGLKVAVATVGAVLPGDFKIKAAKLRGESSEGMLCSFSELAISDDHDGIIELPADAPIGVDVREYLHLDDKTIEI
SVTPNRADCLGIIGVARDVAVVNQLPLTEPDMAEVVASINDTLPIRVDAPQACPRYLGRVVKGINVKAATPLWMREKLRR
CGIRSIDPVVDVTNFVLLELGQPMHAFDLDRISGGVIVRLATDGEELTLLDGTKAKLNADTLVIADHQKPLAMAGIFGGE
HSGVNEETRNVLLESAFFNPLSITGRARRHGLHTDASHRYERGVDPALQHKAIERATRLLIDICGGEAGPVVDVTTASEL
PKQATITLRREKLDRLIGHHIPSEQVSDILRRLGCKVTECGSDWQAVAPSWRFDMAIEEDLVEEVARIYGYNNIPDVPVR
ADLVMTKHREASLSLKRVKTALVDRGYQEAITYSFVDPKVQALLHPQQEALILPNPISVDMSAMRLSLLTGLLSAVVYNQ
NRQQSRLRLFESGLRFVPDNTADLGIRQDVMLAGVIAGHRYDEHWDLARQPIDFYDLKGDLEAILELTGKLSDVQFRAEA
HSALHPGQSAAIYLAGEHIGFIGVVHPELERKLDLNGRTVVFEVQWNKLADRAVPQAREISRFPANRRDIAVVVAENVPA
EDILAECKKVGANQVVGVNLFDVYSGKGVAEGYKSLAISLVLQDTARTLEEEEIAATVAKCVEALKQRFQASLRD

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI124028525, Length=384, Percent_Identity=22.3958333333333, Blast_Score=67, Evalue=7e-11,
Organism=Escherichia coli, GI1788006, Length=795, Percent_Identity=83.3962264150943, Blast_Score=1344, Evalue=0.0,
Organism=Drosophila melanogaster, GI21355211, Length=383, Percent_Identity=22.4543080939948, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFB_YERPE (Q8ZDX1)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AD0296
- RefSeq:   NP_669225.1
- RefSeq:   NP_993544.1
- RefSeq:   YP_002347391.1
- ProteinModelPortal:   Q8ZDX1
- SMR:   Q8ZDX1
- GeneID:   1146856
- GeneID:   1175261
- GeneID:   2764903
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO2428
- KEGG:   ypk:y1909
- KEGG:   ypm:YP_2215
- HOGENOM:   HBG399638
- OMA:   ADKLRVC
- ProtClustDB:   PRK00629
- BioCyc:   YPES187410:Y1909-MONOMER
- BioCyc:   YPES214092:YPO2428-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00283
- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547
- Gene3D:   G3DSA:3.50.40.10
- Gene3D:   G3DSA:3.30.56.20
- Gene3D:   G3DSA:3.30.70.380
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00873
- SMART:   SM00874
- SMART:   SM00896
- TIGRFAMs:   TIGR00472

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind; SSF56037 B3_4; SSF54991 Fdx_AntiC_bd; SSF50249 Nucleic_acid_OB; SSF46955 Putativ_DNA_bind

EC number: =6.1.1.20

Molecular weight: Translated: 87031; Mature: 87031

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS51483 B5; PS51447 FDX_ACB; PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSELWLREWVNPAISSDDLAHQITMAGLEVDGVDAVAGEFNGVVVGHVVECGQHPNAD
CCHHHHHHHHHCCCCCCCCHHHHHHEEECCEECCCHHHHCCCCCEEEEEHHHCCCCCCCC
KLRVTKIDVGGDRLLDIVCGAPNCRQGLKVAVATVGAVLPGDFKIKAAKLRGESSEGMLC
CEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEE
SFSELAISDDHDGIIELPADAPIGVDVREYLHLDDKTIEISVTPNRADCLGIIGVARDVA
EHHHHEECCCCCCEEEECCCCCCCCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHH
VVNQLPLTEPDMAEVVASINDTLPIRVDAPQACPRYLGRVVKGINVKAATPLWMREKLRR
HHHCCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
CGIRSIDPVVDVTNFVLLELGQPMHAFDLDRISGGVIVRLATDGEELTLLDGTKAKLNAD
CCCCCCCHHHHHHHHHHHHCCCCCHHHCHHHCCCCEEEEEECCCCEEEEECCCCCEECCC
TLVIADHQKPLAMAGIFGGEHSGVNEETRNVLLESAFFNPLSITGRARRHGLHTDASHRY
EEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEECCHHHHCCCCCCCHHHH
ERGVDPALQHKAIERATRLLIDICGGEAGPVVDVTTASELPKQATITLRREKLDRLIGHH
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHCCCHHEEEEHHHHHHHHHHCC
IPSEQVSDILRRLGCKVTECGSDWQAVAPSWRFDMAIEEDLVEEVARIYGYNNIPDVPVR
CCHHHHHHHHHHHCCEEEECCCCCHHCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCE
ADLVMTKHREASLSLKRVKTALVDRGYQEAITYSFVDPKVQALLHPQQEALILPNPISVD
EEEEEECCCHHHHHHHHHHHHHHHCCHHHHHEEEECCHHHHHHHCCCCCEEECCCCCCCC
MSAMRLSLLTGLLSAVVYNQNRQQSRLRLFESGLRFVPDNTADLGIRQDVMLAGVIAGHR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
YDEHWDLARQPIDFYDLKGDLEAILELTGKLSDVQFRAEAHSALHPGQSAAIYLAGEHIG
CCCCHHHHCCCCCEEECCHHHHHHHHHHCCCCCEEEHHHHHHHCCCCCCEEEEEECCCEE
FIGVVHPELERKLDLNGRTVVFEVQWNKLADRAVPQAREISRFPANRRDIAVVVAENVPA
EEEEECHHHHHHCCCCCCEEEEEEECCHHHHHCCCCHHHHHHCCCCCCCEEEEEECCCCH
EDILAECKKVGANQVVGVNLFDVYSGKGVAEGYKSLAISLVLQDTARTLEEEEIAATVAK
HHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHH
CVEALKQRFQASLRD
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFSELWLREWVNPAISSDDLAHQITMAGLEVDGVDAVAGEFNGVVVGHVVECGQHPNAD
CCHHHHHHHHHCCCCCCCCHHHHHHEEECCEECCCHHHHCCCCCEEEEEHHHCCCCCCCC
KLRVTKIDVGGDRLLDIVCGAPNCRQGLKVAVATVGAVLPGDFKIKAAKLRGESSEGMLC
CEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEE
SFSELAISDDHDGIIELPADAPIGVDVREYLHLDDKTIEISVTPNRADCLGIIGVARDVA
EHHHHEECCCCCCEEEECCCCCCCCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHH
VVNQLPLTEPDMAEVVASINDTLPIRVDAPQACPRYLGRVVKGINVKAATPLWMREKLRR
HHHCCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
CGIRSIDPVVDVTNFVLLELGQPMHAFDLDRISGGVIVRLATDGEELTLLDGTKAKLNAD
CCCCCCCHHHHHHHHHHHHCCCCCHHHCHHHCCCCEEEEEECCCCEEEEECCCCCEECCC
TLVIADHQKPLAMAGIFGGEHSGVNEETRNVLLESAFFNPLSITGRARRHGLHTDASHRY
EEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEECCHHHHCCCCCCCHHHH
ERGVDPALQHKAIERATRLLIDICGGEAGPVVDVTTASELPKQATITLRREKLDRLIGHH
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHCCCHHEEEEHHHHHHHHHHCC
IPSEQVSDILRRLGCKVTECGSDWQAVAPSWRFDMAIEEDLVEEVARIYGYNNIPDVPVR
CCHHHHHHHHHHHCCEEEECCCCCHHCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCE
ADLVMTKHREASLSLKRVKTALVDRGYQEAITYSFVDPKVQALLHPQQEALILPNPISVD
EEEEEECCCHHHHHHHHHHHHHHHCCHHHHHEEEECCHHHHHHHCCCCCEEECCCCCCCC
MSAMRLSLLTGLLSAVVYNQNRQQSRLRLFESGLRFVPDNTADLGIRQDVMLAGVIAGHR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
YDEHWDLARQPIDFYDLKGDLEAILELTGKLSDVQFRAEAHSALHPGQSAAIYLAGEHIG
CCCCHHHHCCCCCEEECCHHHHHHHHHHCCCCCEEEHHHHHHHCCCCCCEEEEEECCCEE
FIGVVHPELERKLDLNGRTVVFEVQWNKLADRAVPQAREISRFPANRRDIAVVVAENVPA
EEEEECHHHHHHCCCCCCEEEEEEECCHHHHHCCCCHHHHHHCCCCCCCEEEEEECCCCH
EDILAECKKVGANQVVGVNLFDVYSGKGVAEGYKSLAISLVLQDTARTLEEEEIAATVAK
HHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHH
CVEALKQRFQASLRD
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430