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The map label for this gene is ilvB [H]

Identifier: 218929385

GI number: 218929385

Start: 2578383

End: 2580065

Strand: Direct

Name: ilvB [H]

Synonym: YPO2293

Alternate gene names: 218929385

Gene position: 2578383-2580065 (Clockwise)

Preceding gene: 218929383

Following gene: 218929386

Centisome position: 55.4

GC content: 49.85

Gene sequence:

>1683_bases
ATGAAGGATATAACTATGCGTTATACAGGCGCCCAATTGATAGTTCGGTTACTGGAACAACAAGGCATTACTCTTGTAAG
CGGTATTCCCGGCGGTGCAGCCTTACCGCTGTATGATGCCCTAGGGCAAAGCCGTATTATTCGTCATGTATTGGCACGAC
ATGAGCAAGGTGCGGGTTTTATGGCTCAGGGAATGGCCCGAGCAACCGGTGAAACGGCAGTTTGTTTAGCCTCCAGTGGG
CCAGGTGCAACCAATTTAGTGACCGCGATTGCCGATGCCAAACTTGATTCAATCCCATTAGTTTGTATCACTGGCCAAGT
CCCATCTTCTATGATCGGTACCGATGCGTTTCAAGAAGTTGATACTTACGGCATCTCAATACCCATCACTAAACACAACT
ACCTCGTCCGCGATATCAGCGAGTTGGCGCAGGTTATTCCACAAGCGTTCCGGATTGCTCAATCAGGGCGGCCCGGCCCG
GTATGGATCGATATTCCCAAAGATGTACAAACGGCGGAAATTGACTTAGCTACCCTGCCACCACCGGGAATTGCCGATAC
TCCTGGCCCAGTAGACCTCTCTGCTATCGCGCAAGTCGCCAAGATGATTAACCAATCTGTACGGCCAGTGCTCTATTTAG
GTGGGGGAATTGTCAGTTCAGGGGCGCATCAGCAAGCAATACAATTGGCGGAACTGGCAAGTCTGCCGACGACAATGACG
TTAATGGCATTAGGCACAATGCCCGTTGACCACCCATTATCACTGGGCATGTTGGGTATGCATGCGGCTCGCTCGACCAA
CCTGATTATGCAACAAGCGGATTTATTGATTGTTCTCGGCGCACGGTTTGATGATCGGGCCATCGGAAAGGCAGAACAAT
TCTGCCCTCATGCCAATATTATTCATATTGATATTGACCCGGCAGAGTTGGGGAAAATCCGTTGCCCACATTTGGCAATG
AATGCCGATATTGCGCAGGTATTGACACATTTATTGCCAATGATCGACGCTCAGCTACGCAGCGATTGGCGTTCAACTGT
TGCCGATATGCAGCGTGAATTTCCATTCAACCAACCCAATAGCGAAAATCCACTGTGCCATTATGGGTTGATTCGTGCTG
CTGCCGAAGCACTGGATGACGAGACCATTATTACCACCGATGTCGGGCAGCATCAGATGTGGGTGGCACAGGCTTATCCA
CTGCATCGCCCACGCCAATGGTTAACCTCCGGCGGGCTAGGAACCATGGGCTTTGGCTTACCCGCCGCGATTGGTGCCGC
TCTGGCAAAACCAGAAAGTAAAGTGGTCTGCTTCTCTGGTGACGGAAGCCTAATGATGAATATTCAGGAGATGGCGACCG
CGGCTGAAGAGCAACTGAATATAAAAATTATTTTGATGAATAACCAATCGCTGGGATTAGTCCATCAACAACAGGACCTT
TTCTTCGGCAAACGTATTTTTGCTGCTGATTACGCTTATCGCACTAACTTCATTCATATCGCCGAGGGATTCGGTTTCTC
TACCTGCGACCTTAATACTGCCAGCGATCCTTATACTGCACTGCATGAAGCCTTAAATCGCCCCGGTCCGGTGCTTATCC
ACGCACTGATCAATGTGGATGAGAAAGTTTACCCAATGGTGCCACCCGGCGCGGCAAATATCGATATGATTGGAGGGGAA
TAG

Upstream 100 bases:

>100_bases
CCGTAACGGGTACCGAATCCAGAAAGATTCCCAAACCCCGCCGGCGCCAGCCGAGCGGGGTTTCTTTTTATCGCCCCTCT
CAGTTACCGCACCGGCCCTG

Downstream 100 bases:

>100_bases
TATGATAAATCAACCAATACCCACTCGGGTCACTCTGCTCCTGACGGTGCGTAACCATCCAGGTGTGATGTCACATATCT
GTGGCTTATTTGCCCGCCGC

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS-I; Acetohydroxy-acid synthase I large subunit; ALS-I [H]

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MKDITMRYTGAQLIVRLLEQQGITLVSGIPGGAALPLYDALGQSRIIRHVLARHEQGAGFMAQGMARATGETAVCLASSG
PGATNLVTAIADAKLDSIPLVCITGQVPSSMIGTDAFQEVDTYGISIPITKHNYLVRDISELAQVIPQAFRIAQSGRPGP
VWIDIPKDVQTAEIDLATLPPPGIADTPGPVDLSAIAQVAKMINQSVRPVLYLGGGIVSSGAHQQAIQLAELASLPTTMT
LMALGTMPVDHPLSLGMLGMHAARSTNLIMQQADLLIVLGARFDDRAIGKAEQFCPHANIIHIDIDPAELGKIRCPHLAM
NADIAQVLTHLLPMIDAQLRSDWRSTVADMQREFPFNQPNSENPLCHYGLIRAAAEALDDETIITTDVGQHQMWVAQAYP
LHRPRQWLTSGGLGTMGFGLPAAIGAALAKPESKVVCFSGDGSLMMNIQEMATAAEEQLNIKIILMNNQSLGLVHQQQDL
FFGKRIFAADYAYRTNFIHIAEGFGFSTCDLNTASDPYTALHEALNRPGPVLIHALINVDEKVYPMVPPGAANIDMIGGE

Sequences:

>Translated_560_residues
MKDITMRYTGAQLIVRLLEQQGITLVSGIPGGAALPLYDALGQSRIIRHVLARHEQGAGFMAQGMARATGETAVCLASSG
PGATNLVTAIADAKLDSIPLVCITGQVPSSMIGTDAFQEVDTYGISIPITKHNYLVRDISELAQVIPQAFRIAQSGRPGP
VWIDIPKDVQTAEIDLATLPPPGIADTPGPVDLSAIAQVAKMINQSVRPVLYLGGGIVSSGAHQQAIQLAELASLPTTMT
LMALGTMPVDHPLSLGMLGMHAARSTNLIMQQADLLIVLGARFDDRAIGKAEQFCPHANIIHIDIDPAELGKIRCPHLAM
NADIAQVLTHLLPMIDAQLRSDWRSTVADMQREFPFNQPNSENPLCHYGLIRAAAEALDDETIITTDVGQHQMWVAQAYP
LHRPRQWLTSGGLGTMGFGLPAAIGAALAKPESKVVCFSGDGSLMMNIQEMATAAEEQLNIKIILMNNQSLGLVHQQQDL
FFGKRIFAADYAYRTNFIHIAEGFGFSTCDLNTASDPYTALHEALNRPGPVLIHALINVDEKVYPMVPPGAANIDMIGGE
>Mature_560_residues
MKDITMRYTGAQLIVRLLEQQGITLVSGIPGGAALPLYDALGQSRIIRHVLARHEQGAGFMAQGMARATGETAVCLASSG
PGATNLVTAIADAKLDSIPLVCITGQVPSSMIGTDAFQEVDTYGISIPITKHNYLVRDISELAQVIPQAFRIAQSGRPGP
VWIDIPKDVQTAEIDLATLPPPGIADTPGPVDLSAIAQVAKMINQSVRPVLYLGGGIVSSGAHQQAIQLAELASLPTTMT
LMALGTMPVDHPLSLGMLGMHAARSTNLIMQQADLLIVLGARFDDRAIGKAEQFCPHANIIHIDIDPAELGKIRCPHLAM
NADIAQVLTHLLPMIDAQLRSDWRSTVADMQREFPFNQPNSENPLCHYGLIRAAAEALDDETIITTDVGQHQMWVAQAYP
LHRPRQWLTSGGLGTMGFGLPAAIGAALAKPESKVVCFSGDGSLMMNIQEMATAAEEQLNIKIILMNNQSLGLVHQQQDL
FFGKRIFAADYAYRTNFIHIAEGFGFSTCDLNTASDPYTALHEALNRPGPVLIHALINVDEKVYPMVPPGAANIDMIGGE

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=496, Percent_Identity=27.6209677419355, Blast_Score=158, Evalue=2e-38,
Organism=Homo sapiens, GI21361361, Length=581, Percent_Identity=24.7848537005164, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1790104, Length=552, Percent_Identity=69.5652173913043, Blast_Score=799, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=554, Percent_Identity=40.072202166065, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI1786717, Length=548, Percent_Identity=33.9416058394161, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1787096, Length=551, Percent_Identity=28.8566243194192, Blast_Score=195, Evalue=8e-51,
Organism=Escherichia coli, GI1788716, Length=566, Percent_Identity=25.9717314487633, Blast_Score=142, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17531299, Length=566, Percent_Identity=26.3250883392226, Blast_Score=154, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17531301, Length=566, Percent_Identity=26.3250883392226, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17542570, Length=592, Percent_Identity=24.8310810810811, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=571, Percent_Identity=39.2294220665499, Blast_Score=398, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6320816, Length=559, Percent_Identity=26.1180679785331, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6323163, Length=496, Percent_Identity=22.3790322580645, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19922626, Length=492, Percent_Identity=29.0650406504065, Blast_Score=169, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60053; Mature: 60053

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDITMRYTGAQLIVRLLEQQGITLVSGIPGGAALPLYDALGQSRIIRHVLARHEQGAGF
CCCEEEEECHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHCHHHHHHHHHHHCCCCCCH
MAQGMARATGETAVCLASSGPGATNLVTAIADAKLDSIPLVCITGQVPSSMIGTDAFQEV
HHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHCCHHHHHH
DTYGISIPITKHNYLVRDISELAQVIPQAFRIAQSGRPGPVWIDIPKDVQTAEIDLATLP
HHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEEEECC
PPGIADTPGPVDLSAIAQVAKMINQSVRPVLYLGGGIVSSGAHQQAIQLAELASLPTTMT
CCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHCCCCHHHHHHHHHHHCCCHHHH
LMALGTMPVDHPLSLGMLGMHAARSTNLIMQQADLLIVLGARFDDRAIGKAEQFCPHANI
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHCCCCCE
IHIDIDPAELGKIRCPHLAMNADIAQVLTHLLPMIDAQLRSDWRSTVADMQREFPFNQPN
EEEECCHHHHCCEECCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SENPLCHYGLIRAAAEALDDETIITTDVGQHQMWVAQAYPLHRPRQWLTSGGLGTMGFGL
CCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCH
PAAIGAALAKPESKVVCFSGDGSLMMNIQEMATAAEEQLNIKIILMNNQSLGLVHQQQDL
HHHHHHHHCCCCCCEEEECCCCCEEEEHHHHHHHHHHHCEEEEEEECCCCCCEEECHHHH
FFGKRIFAADYAYRTNFIHIAEGFGFSTCDLNTASDPYTALHEALNRPGPVLIHALINVD
HHHHHHHHHHHHHHCCEEEEECCCCEEEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCC
EKVYPMVPPGAANIDMIGGE
CCEECCCCCCCCCEEECCCC
>Mature Secondary Structure
MKDITMRYTGAQLIVRLLEQQGITLVSGIPGGAALPLYDALGQSRIIRHVLARHEQGAGF
CCCEEEEECHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHCHHHHHHHHHHHCCCCCCH
MAQGMARATGETAVCLASSGPGATNLVTAIADAKLDSIPLVCITGQVPSSMIGTDAFQEV
HHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHCCHHHHHH
DTYGISIPITKHNYLVRDISELAQVIPQAFRIAQSGRPGPVWIDIPKDVQTAEIDLATLP
HHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEEEECC
PPGIADTPGPVDLSAIAQVAKMINQSVRPVLYLGGGIVSSGAHQQAIQLAELASLPTTMT
CCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHCCCCHHHHHHHHHHHCCCHHHH
LMALGTMPVDHPLSLGMLGMHAARSTNLIMQQADLLIVLGARFDDRAIGKAEQFCPHANI
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHCCCCCE
IHIDIDPAELGKIRCPHLAMNADIAQVLTHLLPMIDAQLRSDWRSTVADMQREFPFNQPN
EEEECCHHHHCCEECCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SENPLCHYGLIRAAAEALDDETIITTDVGQHQMWVAQAYPLHRPRQWLTSGGLGTMGFGL
CCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCH
PAAIGAALAKPESKVVCFSGDGSLMMNIQEMATAAEEQLNIKIILMNNQSLGLVHQQQDL
HHHHHHHHCCCCCCEEEECCCCCEEEEHHHHHHHHHHHCEEEEEEECCCCCCEEECHHHH
FFGKRIFAADYAYRTNFIHIAEGFGFSTCDLNTASDPYTALHEALNRPGPVLIHALINVD
HHHHHHHHHHHHHHCCEEEEECCCCEEEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCC
EKVYPMVPPGAANIDMIGGE
CCEECCCCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2989782; 2989781; 7686882; 9278503 [H]