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The map label for this gene is hemK [H]

Identifier: 218929129

GI number: 218929129

Start: 2290317

End: 2291147

Strand: Direct

Name: hemK [H]

Synonym: YPO2018

Alternate gene names: 218929129

Gene position: 2290317-2291147 (Clockwise)

Preceding gene: 218929128

Following gene: 218929130

Centisome position: 49.21

GC content: 53.55

Gene sequence:

>831_bases
ATGAATTATCAGCATTGGCTGTCGTTGGCGGCAGCCCGTTTTACCCACAGTGACAGCCCTAAGCGTGATGCTGAAATCCT
ACTGAGTTTTGTGACAGGTAAGGCCCGGACCTATCTGTTGGCTTTTGGTGAAACGGAAATAACGGCAGAACAACTACTGT
GGCTCGAAACACTGGCGAACCGTCGGGAGCAAGGTGAACCCATTGCTTATCTGGTTGGGGAGCGCGAGTTCTGGTCTTTA
CCGCTGAGTGTTTCCTCTGCAACGCTTATCCCACGGCCTGACACCGAATGTCTGGTGGAGCAGGCGCTGGCACGTTTACC
CGCAATGCCTTGTCGTATTTTGGACCTGGGGACGGGTACCGGTGCAATCGCGTTGGCACTGGCCACTGAACGGCGTGATT
GCGCGGTCATCGCCGTAGATATCAACGCTGATGCTGTCGCTTTGGCCCGCCATAATGCAGAAAAACTCACGATAGATAAT
GTGTGTTTTTTGCAAGGCAGTTGGTTTGAGCCAGTGAGCGGGCGTTTTGCGCTTATCGCCAGTAATCCCCCTTATATTGA
TGCCAATGACCCTCATCTTAATGAAGGCGATGTGCGCTATGAACCGCATAGTGCATTGGTTGCGGCGGCTGAGGGGATGG
CCGATCTGGCCGCGATTGTCAGCCAGGCACCGGGCTACTTGGATCCCGGCGGTTGGCTCATGCTAGAGCATGGCTGGCAG
CAGGCCAATGCAGTGCAAGAACTGCTTAAAAACTCGGGATTTAGTGCGGTAATGACCTGTAAAGATTATGGCAATAATGA
CCGGGTGACATTGGGGCAATGGGCCGTGTAA

Upstream 100 bases:

>100_bases
GGCTTGATGAAGTGATGGAAGGTAAACTGGATATGCTCATCCAGCCCATTGTGCAGGAATATCAAGCTGACCAACTGTCT
GCATTGTCAGAGCAAGACTA

Downstream 100 bases:

>100_bases
CCACGCGCAGCTAATAGCGCTGCAACATCAAGTAAAAAGAGATAAAACAAGGAATCGATAGTCATGTGGGTTGATTACAT
CGCCATGAAATATTTGCATT

Product: N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase

Products: NA

Alternate protein names: M.EcoKHemKP; Protein-(glutamine-N(5)) MTase hemK; Protein-glutamine N-methyltransferase hemK [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MNYQHWLSLAAARFTHSDSPKRDAEILLSFVTGKARTYLLAFGETEITAEQLLWLETLANRREQGEPIAYLVGEREFWSL
PLSVSSATLIPRPDTECLVEQALARLPAMPCRILDLGTGTGAIALALATERRDCAVIAVDINADAVALARHNAEKLTIDN
VCFLQGSWFEPVSGRFALIASNPPYIDANDPHLNEGDVRYEPHSALVAAAEGMADLAAIVSQAPGYLDPGGWLMLEHGWQ
QANAVQELLKNSGFSAVMTCKDYGNNDRVTLGQWAV

Sequences:

>Translated_276_residues
MNYQHWLSLAAARFTHSDSPKRDAEILLSFVTGKARTYLLAFGETEITAEQLLWLETLANRREQGEPIAYLVGEREFWSL
PLSVSSATLIPRPDTECLVEQALARLPAMPCRILDLGTGTGAIALALATERRDCAVIAVDINADAVALARHNAEKLTIDN
VCFLQGSWFEPVSGRFALIASNPPYIDANDPHLNEGDVRYEPHSALVAAAEGMADLAAIVSQAPGYLDPGGWLMLEHGWQ
QANAVQELLKNSGFSAVMTCKDYGNNDRVTLGQWAV
>Mature_276_residues
MNYQHWLSLAAARFTHSDSPKRDAEILLSFVTGKARTYLLAFGETEITAEQLLWLETLANRREQGEPIAYLVGEREFWSL
PLSVSSATLIPRPDTECLVEQALARLPAMPCRILDLGTGTGAIALALATERRDCAVIAVDINADAVALARHNAEKLTIDN
VCFLQGSWFEPVSGRFALIASNPPYIDANDPHLNEGDVRYEPHSALVAAAEGMADLAAIVSQAPGYLDPGGWLMLEHGWQ
QANAVQELLKNSGFSAVMTCKDYGNNDRVTLGQWAV

Specific function: Methylates the translation termination release factor RF1/PrfA on 'Gln-235' and RF2/PrfB on 'Gln-252' [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=236, Percent_Identity=32.6271186440678, Blast_Score=93, Evalue=2e-19,
Organism=Escherichia coli, GI1787463, Length=275, Percent_Identity=62.5454545454545, Blast_Score=356, Evalue=1e-99,
Organism=Escherichia coli, GI87082085, Length=203, Percent_Identity=31.5270935960591, Blast_Score=90, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24582226, Length=219, Percent_Identity=26.027397260274, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 30121; Mature: 30121

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYQHWLSLAAARFTHSDSPKRDAEILLSFVTGKARTYLLAFGETEITAEQLLWLETLAN
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHH
RREQGEPIAYLVGEREFWSLPLSVSSATLIPRPDTECLVEQALARLPAMPCRILDLGTGT
HHHCCCCEEEEECCCEEEECCEECCCEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCC
GAIALALATERRDCAVIAVDINADAVALARHNAEKLTIDNVCFLQGSWFEPVSGRFALIA
CEEEEEEEECCCCEEEEEEECCCCEEEEECCCCCEEEECCEEEEECCCCCCCCCCEEEEE
SNPPYIDANDPHLNEGDVRYEPHSALVAAAEGMADLAAIVSQAPGYLDPGGWLMLEHGWQ
CCCCEECCCCCCCCCCCEEECCCHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEECCCC
QANAVQELLKNSGFSAVMTCKDYGNNDRVTLGQWAV
HHHHHHHHHHCCCCEEEEEEECCCCCCCEEECCCCC
>Mature Secondary Structure
MNYQHWLSLAAARFTHSDSPKRDAEILLSFVTGKARTYLLAFGETEITAEQLLWLETLAN
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHH
RREQGEPIAYLVGEREFWSLPLSVSSATLIPRPDTECLVEQALARLPAMPCRILDLGTGT
HHHCCCCEEEEECCCEEEECCEECCCEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCC
GAIALALATERRDCAVIAVDINADAVALARHNAEKLTIDNVCFLQGSWFEPVSGRFALIA
CEEEEEEEECCCCEEEEEEECCCCEEEEECCCCCEEEECCEEEEECCCCCCCCCCEEEEE
SNPPYIDANDPHLNEGDVRYEPHSALVAAAEGMADLAAIVSQAPGYLDPGGWLMLEHGWQ
CCCCEECCCCCCCCCCCEEECCCHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEECCCC
QANAVQELLKNSGFSAVMTCKDYGNNDRVTLGQWAV
HHHHHHHHHHCCCCEEEEEEECCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7883187; 7543480; 11805295; 8905232; 9278503 [H]