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The map label for this gene is thiK [H]

Identifier: 218928750

GI number: 218928750

Start: 1836616

End: 1837422

Strand: Direct

Name: thiK [H]

Synonym: YPO1614

Alternate gene names: 218928750

Gene position: 1836616-1837422 (Clockwise)

Preceding gene: 218928749

Following gene: 218928751

Centisome position: 39.47

GC content: 50.19

Gene sequence:

>807_bases
GTGAAAACCGCCGATTGTCATATTAGCCCGGTATCAGGATTAACCGGTGAAAGTTGGCGTATTACTGGCCCAGGGATTGA
CTGGCTGGCGCGTGAGCAATCGCCGCACAAAAGTCAGTTGGGGGTCAACCGACGACGTGAGCGTAAATTCCTGCAACATA
TTGCTGGCAATGGGCTGTCTCCAGCGGTGATTGCTGCTAATCAGCGTTGGCTAGTGGTGAACTGGCTTGAAGGTGACGTT
GTCACTAATGAGCAGTTCATCCCATTGGTGAATCATGGGCAATTAGCGCGGTTACTGGCCCGCTTGCATCATTTGCCCGC
GAGTGGTTACCGTCTGGATTTGCGTGCTCAGATTGCCCGCTATGGGGCGCTTATTGATCCTACGCGCCGTTCACCTGGCG
GAGTGCGGCTACAGCATGATTTTCTTCGTAGGCCATTACCAGCGATAACAAAAATTGCTCCTTTGCATATGGATATTCAC
CCCGGCAATTTGTTAACAACCCCCGTTGGGCTGAAATTAATTGACTGGGAATATGCGGCAGATGGCGATATTGCTTTGGA
AATTGCCGCGCTATTTCGCGGTAATCATTGGTCAATGCTACAACAACAGGCTTTTTTGCAGGACTATTGCAACAATGAGC
ACGGTTATCATGACATCGCTCGTTTATCCCGTCAGATTCAGCAATGGTTGCCCTGGGTCGATTACCTGATGTTGATGTGG
TTTGAAGTGCGTTGGCAGCAAACCGCGGACCCAATATTCTTGGCATGGGCCGCGCCGTTGCGCCAACGTTTTAATTTGTC
TTTTTAA

Upstream 100 bases:

>100_bases
TTGATGCAGACACAAACCGGTGAAATCATTTGGTCTGGTAATGCGCCTGTCCAATATTGATTCTTCTCTGTCTGCCTTAA
TAGCAAACATCAATCCGGCG

Downstream 100 bases:

>100_bases
GCCCTGCGGCACCAAGCCCGCCGCTTCAAGCACGAAGGGTCGTTATCAATCTGAATAAAGTGAGGTGACCGTGGGTCCCG
TGATGTTAGATGTTGCCAGC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKTADCHISPVSGLTGESWRITGPGIDWLAREQSPHKSQLGVNRRRERKFLQHIAGNGLSPAVIAANQRWLVVNWLEGDV
VTNEQFIPLVNHGQLARLLARLHHLPASGYRLDLRAQIARYGALIDPTRRSPGGVRLQHDFLRRPLPAITKIAPLHMDIH
PGNLLTTPVGLKLIDWEYAADGDIALEIAALFRGNHWSMLQQQAFLQDYCNNEHGYHDIARLSRQIQQWLPWVDYLMLMW
FEVRWQQTADPIFLAWAAPLRQRFNLSF

Sequences:

>Translated_268_residues
MKTADCHISPVSGLTGESWRITGPGIDWLAREQSPHKSQLGVNRRRERKFLQHIAGNGLSPAVIAANQRWLVVNWLEGDV
VTNEQFIPLVNHGQLARLLARLHHLPASGYRLDLRAQIARYGALIDPTRRSPGGVRLQHDFLRRPLPAITKIAPLHMDIH
PGNLLTTPVGLKLIDWEYAADGDIALEIAALFRGNHWSMLQQQAFLQDYCNNEHGYHDIARLSRQIQQWLPWVDYLMLMW
FEVRWQQTADPIFLAWAAPLRQRFNLSF
>Mature_268_residues
MKTADCHISPVSGLTGESWRITGPGIDWLAREQSPHKSQLGVNRRRERKFLQHIAGNGLSPAVIAANQRWLVVNWLEGDV
VTNEQFIPLVNHGQLARLLARLHHLPASGYRLDLRAQIARYGALIDPTRRSPGGVRLQHDFLRRPLPAITKIAPLHMDIH
PGNLLTTPVGLKLIDWEYAADGDIALEIAALFRGNHWSMLQQQAFLQDYCNNEHGYHDIARLSRQIQQWLPWVDYLMLMW
FEVRWQQTADPIFLAWAAPLRQRFNLSF

Specific function: Catalyzes the phosphorylation of thiamine to thiamine phosphate [H]

COG id: COG0510

COG function: function code M; Predicted choline kinase involved in LPS biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787349, Length=248, Percent_Identity=37.5, Blast_Score=134, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002575
- InterPro:   IPR011009
- InterPro:   IPR014093 [H]

Pfam domain/function: PF01636 APH [H]

EC number: =2.7.1.89 [H]

Molecular weight: Translated: 30746; Mature: 30746

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTADCHISPVSGLTGESWRITGPGIDWLAREQSPHKSQLGVNRRRERKFLQHIAGNGLS
CCCCCCCCCCCCCCCCCCEEEECCCCHHHHCCCCCCHHHHCCCHHHHHHHHHHHHCCCCC
PAVIAANQRWLVVNWLEGDVVTNEQFIPLVNHGQLARLLARLHHLPASGYRLDLRAQIAR
CEEEECCCCEEEEEEECCCEECCCCEEEEECCCHHHHHHHHHHCCCCCCEEEHHHHHHHH
YGALIDPTRRSPGGVRLQHDFLRRPLPAITKIAPLHMDIHPGNLLTTPVGLKLIDWEYAA
HCCCCCCCCCCCCCEEEEHHHHHCCCHHHHHHCCEEEEECCCCEEECCCCEEEEEEEECC
DGDIALEIAALFRGNHWSMLQQQAFLQDYCNNEHGYHDIARLSRQIQQWLPWVDYLMLMW
CCCHHEEEEEHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
FEVRWQQTADPIFLAWAAPLRQRFNLSF
HHHHHHCCCCCEEEEEHHHHHHHCCCCC
>Mature Secondary Structure
MKTADCHISPVSGLTGESWRITGPGIDWLAREQSPHKSQLGVNRRRERKFLQHIAGNGLS
CCCCCCCCCCCCCCCCCCEEEECCCCHHHHCCCCCCHHHHCCCHHHHHHHHHHHHCCCCC
PAVIAANQRWLVVNWLEGDVVTNEQFIPLVNHGQLARLLARLHHLPASGYRLDLRAQIAR
CEEEECCCCEEEEEEECCCEECCCCEEEEECCCHHHHHHHHHHCCCCCCEEEHHHHHHHH
YGALIDPTRRSPGGVRLQHDFLRRPLPAITKIAPLHMDIHPGNLLTTPVGLKLIDWEYAA
HCCCCCCCCCCCCCEEEEHHHHHCCCHHHHHHCCEEEEECCCCEEECCCCEEEEEEEECC
DGDIALEIAALFRGNHWSMLQQQAFLQDYCNNEHGYHDIARLSRQIQQWLPWVDYLMLMW
CCCHHEEEEEHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
FEVRWQQTADPIFLAWAAPLRQRFNLSF
HHHHHHCCCCCEEEEEHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA