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The map label for this gene is macA

Identifier: 218928512

GI number: 218928512

Start: 1531046

End: 1532161

Strand: Direct

Name: macA

Synonym: YPO1364

Alternate gene names: 218928512

Gene position: 1531046-1532161 (Clockwise)

Preceding gene: 218928510

Following gene: 218928513

Centisome position: 32.9

GC content: 49.01

Gene sequence:

>1116_bases
TTGATGCAACTCAGTAGAGGTCAGCGCCGGTGGCTTGCCGCCATTGCGGTATTGCTTATCGGTGGTTTTTTCATCGCCAG
GCATCTGATGGCACCGGTACCTGTTAACTATCAAACAGTCAAAGTGGTTCACCGTGATCTGCAACAAAATGTCTTGGCAA
CCGGTAAGCTCGATGCGGTTCGTAAAGTTGATGTAGGTGCGCAGGTCAGTGGTCAGTTGGAAAAACTGTATGTAGAAATT
GGCGATCACGTTAAGCGGGGGCAGTTGCTGGCGATGATCGATCCACAGCAGGCGCAAAACCAAATTAAGGAAGTGGAAGC
CACATTACAGGATTTGAACGCTCAACGGATACAGGCGAAGGCCGAATTACACTTGGCAACGGTTACGCTCGGTCGTCAAC
AAAATTTGGCTAAATTACAGGTGGTGTCACGGCAGGATCTGGACCAGGCAGTAACCGATTTGGCGGTTAAAAATGCGAAA
GTTGGCACGATTGATGCTCAAATCAACAAAGCGAAAGCCAGCTTAGATACGGCCAAAATCAATCTGGATTACACTCAAAT
TTCTGCACCGATGGATGGTGATGTTGTACAAATCACCACGTTGCAAGGTCAGACGGTAATTGCCGCACAGCAAGCGCCCA
ATATTCTGACCCTGGCCGATATGAGTACCATGTTGGTACAAGCACAGGTATCAGAAGCGGATGTTATCAACCTGAAACCG
GGGATGAAAGCCTCGTTCACGGTACTTGGCGATCCTGGCAAGCGTTTTTCTGGCGTACTAAAAGATATTCTGCCAACGCC
AGAGAAAGTCAATGACGCCATCTTTTATTCAGCCCGCTTTGAAGTACCAAACCCTGACAGATTATTGCGGTTACAAATGA
CGGCTCAGGTATCCATACAGCTAGCAAATGTAGATCAGGCCGTGGTGATCCCACTAGCTGCGCTGGGTGATGAGTTAGGG
AGTAATCGCTATCAGGTTACGGTCCTGAAAGAGGGCAAAGAAGAGAAGCGTGAGGTGACCATTGGTATTCGTAATAATGT
CGATGCTCAAGTTATTAGCGGGCTGAGTGTCGGCGAAGACGTCATCGTCAGCCGCGGTGGCACGGGGGATGCATAA

Upstream 100 bases:

>100_bases
TTGATGGTATTTTCTGCGGGTATATTTTCTTTGTGTTGATATGATCCATTTTTCAACCACTTAACTGCCATGGAAATAAG
AATGAAGGGAGGTAATTGAT

Downstream 100 bases:

>100_bases
TGGCGGCATTGCTTGAATTAGAAGGCATCCGCCGTAGTTATCAGTCTGGCGAAGAAATTGTCGATGTGTTGCAGGATGTC
TCATTGACCATCAATGCGGG

Product: macrolide transporter subunit MacA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MMQLSRGQRRWLAAIAVLLIGGFFIARHLMAPVPVNYQTVKVVHRDLQQNVLATGKLDAVRKVDVGAQVSGQLEKLYVEI
GDHVKRGQLLAMIDPQQAQNQIKEVEATLQDLNAQRIQAKAELHLATVTLGRQQNLAKLQVVSRQDLDQAVTDLAVKNAK
VGTIDAQINKAKASLDTAKINLDYTQISAPMDGDVVQITTLQGQTVIAAQQAPNILTLADMSTMLVQAQVSEADVINLKP
GMKASFTVLGDPGKRFSGVLKDILPTPEKVNDAIFYSARFEVPNPDRLLRLQMTAQVSIQLANVDQAVVIPLAALGDELG
SNRYQVTVLKEGKEEKREVTIGIRNNVDAQVISGLSVGEDVIVSRGGTGDA

Sequences:

>Translated_371_residues
MMQLSRGQRRWLAAIAVLLIGGFFIARHLMAPVPVNYQTVKVVHRDLQQNVLATGKLDAVRKVDVGAQVSGQLEKLYVEI
GDHVKRGQLLAMIDPQQAQNQIKEVEATLQDLNAQRIQAKAELHLATVTLGRQQNLAKLQVVSRQDLDQAVTDLAVKNAK
VGTIDAQINKAKASLDTAKINLDYTQISAPMDGDVVQITTLQGQTVIAAQQAPNILTLADMSTMLVQAQVSEADVINLKP
GMKASFTVLGDPGKRFSGVLKDILPTPEKVNDAIFYSARFEVPNPDRLLRLQMTAQVSIQLANVDQAVVIPLAALGDELG
SNRYQVTVLKEGKEEKREVTIGIRNNVDAQVISGLSVGEDVIVSRGGTGDA
>Mature_371_residues
MMQLSRGQRRWLAAIAVLLIGGFFIARHLMAPVPVNYQTVKVVHRDLQQNVLATGKLDAVRKVDVGAQVSGQLEKLYVEI
GDHVKRGQLLAMIDPQQAQNQIKEVEATLQDLNAQRIQAKAELHLATVTLGRQQNLAKLQVVSRQDLDQAVTDLAVKNAK
VGTIDAQINKAKASLDTAKINLDYTQISAPMDGDVVQITTLQGQTVIAAQQAPNILTLADMSTMLVQAQVSEADVINLKP
GMKASFTVLGDPGKRFSGVLKDILPTPEKVNDAIFYSARFEVPNPDRLLRLQMTAQVSIQLANVDQAVVIPLAALGDELG
SNRYQVTVLKEGKEEKREVTIGIRNNVDAQVISGLSVGEDVIVSRGGTGDA

Specific function: Efflux transporter for macrolide antibiotics

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family

Homologues:

Organism=Escherichia coli, GI87081796, Length=364, Percent_Identity=65.1098901098901, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI87082036, Length=358, Percent_Identity=30.4469273743017, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790519, Length=239, Percent_Identity=30.1255230125523, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789929, Length=246, Percent_Identity=28.4552845528455, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789637, Length=188, Percent_Identity=29.7872340425532, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MACA_YERPE (P58411)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AF0166
- RefSeq:   NP_670114.1
- RefSeq:   YP_002346387.1
- ProteinModelPortal:   P58411
- SMR:   P58411
- IntAct:   P58411
- GeneID:   1147761
- GeneID:   1174205
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO1364
- KEGG:   ypk:y2814
- KEGG:   ypm:YP_1230
- HOGENOM:   HBG651049
- OMA:   AYGVFRI
- ProtClustDB:   PRK11578
- BioCyc:   YPES187410:Y2814-MONOMER
- BioCyc:   YPES214092:YPO1364-MONOMER
- InterPro:   IPR006143
- TIGRFAMs:   TIGR01730

Pfam domain/function: PF00529 HlyD

EC number: NA

Molecular weight: Translated: 40187; Mature: 40187

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQLSRGQRRWLAAIAVLLIGGFFIARHLMAPVPVNYQTVKVVHRDLQQNVLATGKLDAV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHH
RKVDVGAQVSGQLEKLYVEIGDHVKRGQLLAMIDPQQAQNQIKEVEATLQDLNAQRIQAK
EEECCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCHHHHHHH
AELHLATVTLGRQQNLAKLQVVSRQDLDQAVTDLAVKNAKVGTIDAQINKAKASLDTAKI
HEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCEEEE
NLDYTQISAPMDGDVVQITTLQGQTVIAAQQAPNILTLADMSTMLVQAQVSEADVINLKP
EEEHEEECCCCCCCEEEEEEECCCEEEEECCCCCEEEEHHHHHHHHEEECCCCCEEEECC
GMKASFTVLGDPGKRFSGVLKDILPTPEKVNDAIFYSARFEVPNPDRLLRLQMTAQVSIQ
CCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCEEEEEEEEEEEEE
LANVDQAVVIPLAALGDELGSNRYQVTVLKEGKEEKREVTIGIRNNVDAQVISGLSVGED
EECCCCEEEEEHHHHHHHHCCCCEEEEEEECCCCCCEEEEEEECCCCCHHHHCCCCCCCC
VIVSRGGTGDA
EEEECCCCCCC
>Mature Secondary Structure
MMQLSRGQRRWLAAIAVLLIGGFFIARHLMAPVPVNYQTVKVVHRDLQQNVLATGKLDAV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHH
RKVDVGAQVSGQLEKLYVEIGDHVKRGQLLAMIDPQQAQNQIKEVEATLQDLNAQRIQAK
EEECCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCHHHHHHH
AELHLATVTLGRQQNLAKLQVVSRQDLDQAVTDLAVKNAKVGTIDAQINKAKASLDTAKI
HEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCEEEE
NLDYTQISAPMDGDVVQITTLQGQTVIAAQQAPNILTLADMSTMLVQAQVSEADVINLKP
EEEHEEECCCCCCCEEEEEEECCCEEEEECCCCCEEEEHHHHHHHHEEECCCCCEEEECC
GMKASFTVLGDPGKRFSGVLKDILPTPEKVNDAIFYSARFEVPNPDRLLRLQMTAQVSIQ
CCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCEEEEEEEEEEEEE
LANVDQAVVIPLAALGDELGSNRYQVTVLKEGKEEKREVTIGIRNNVDAQVISGLSVGED
EECCCCEEEEEHHHHHHHHCCCCEEEEEEECCCCCCEEEEEEECCCCCHHHHCCCCCCCC
VIVSRGGTGDA
EEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430