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The map label for this gene is potI [H]

Identifier: 218928484

GI number: 218928484

Start: 1499691

End: 1500536

Strand: Direct

Name: potI [H]

Synonym: YPO1334

Alternate gene names: 218928484

Gene position: 1499691-1500536 (Clockwise)

Preceding gene: 218928483

Following gene: 218928485

Centisome position: 32.23

GC content: 53.78

Gene sequence:

>846_bases
ATGAATAACTTACCGGAGGTGCGTTCTGTCTGGCGTCGGGTGATCCTGACCGTCGGTTATACCTTCCTGTATGCCCCAAT
GTTGATGTTGGTAATTTATTCGTTTAACAGCTCGAAGCTGGTGACGGTGTGGGCTGGGTGGTCAACCCGTTGGTATACGC
AGTTATTTAATGATTCGGCGATGATCTCTGCCGTTGGGCTGAGTCTGACGATTGCCGCCGCCTCTGCCACTATGGCGGTG
GTGTTGGGGACGATTGCCGCGGTGGTCATGGTGCGCTTTGGGCGTTTTCGTGGGTCAACCGGTTTTGCTTTTATGCTGAC
TGCACCGCTGGTTATGCCTGATGTCATCACCGGACTGTCACTACTGCTGCTGTTTGTGGCGATGGGGCATGCCATTGGGT
GGCCCGCAGAACGTGGCATGTTCACCATCTGGCTGGCCCATGTGACTTTCTGCACGGCCTATGTGACGGTGGTCATCAGT
TCGCGGTTGCGCGAATTGGACCGTTCTATTGAAGAGGCGGCCATGGATCTGGGCGCGACACCACTGAAGGTCTTCTTTGT
TATTACGGTACCGATGATCGCCCCTGCGCTGATCTCTGGTTGGTTGCTGGCCTTTACCTTATCCCTTGATGATCTGGTTA
TTGCCAGCTTTGTTGCCGGGCCGGGGGCCACCACGTTACCGATGTTGGTATTCTCCAGTGTGCGTATGGGGGTGAATCCA
GAGATTAATGCGTTAGCGACACTTATCCTGCTGGTGGTTGGGGTTATCGGTTTATTTGCATGGTGGATAATGTCGCGTGC
GGAAAAGCAGAGATTGCGCGAATTACGTAAAGCTGCTCGTAGCTGA

Upstream 100 bases:

>100_bases
GGCCGGTGGCCTCTGCGGTGGCAACCGTGATGTTAGTGCTGCTGATTGCCCCGATACTCTGGTTCCACAAACATCAAAAT
AAAGATATTGGGGGCGCGAG

Downstream 100 bases:

>100_bases
TTCTCCCTAAAGCTGTTAGTCTTTCGTCTCCTTTAACGCCGCTTTAGAGCGGCGTTATTGTGAGAGATAACATCAGGTTG
CGGAAAACCGATTATGAGCA

Product: putrescine transporter subunit: membrane component of ABC superfamily

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MNNLPEVRSVWRRVILTVGYTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYTQLFNDSAMISAVGLSLTIAAASATMAV
VLGTIAAVVMVRFGRFRGSTGFAFMLTAPLVMPDVITGLSLLLLFVAMGHAIGWPAERGMFTIWLAHVTFCTAYVTVVIS
SRLRELDRSIEEAAMDLGATPLKVFFVITVPMIAPALISGWLLAFTLSLDDLVIASFVAGPGATTLPMLVFSSVRMGVNP
EINALATLILLVVGVIGLFAWWIMSRAEKQRLRELRKAARS

Sequences:

>Translated_281_residues
MNNLPEVRSVWRRVILTVGYTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYTQLFNDSAMISAVGLSLTIAAASATMAV
VLGTIAAVVMVRFGRFRGSTGFAFMLTAPLVMPDVITGLSLLLLFVAMGHAIGWPAERGMFTIWLAHVTFCTAYVTVVIS
SRLRELDRSIEEAAMDLGATPLKVFFVITVPMIAPALISGWLLAFTLSLDDLVIASFVAGPGATTLPMLVFSSVRMGVNP
EINALATLILLVVGVIGLFAWWIMSRAEKQRLRELRKAARS
>Mature_281_residues
MNNLPEVRSVWRRVILTVGYTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYTQLFNDSAMISAVGLSLTIAAASATMAV
VLGTIAAVVMVRFGRFRGSTGFAFMLTAPLVMPDVITGLSLLLLFVAMGHAIGWPAERGMFTIWLAHVTFCTAYVTVVIS
SRLRELDRSIEEAAMDLGATPLKVFFVITVPMIAPALISGWLLAFTLSLDDLVIASFVAGPGATTLPMLVFSSVRMGVNP
EINALATLILLVVGVIGLFAWWIMSRAEKQRLRELRKAARS

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=281, Percent_Identity=83.9857651245552, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI1787368, Length=239, Percent_Identity=39.7489539748954, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1787714, Length=256, Percent_Identity=33.59375, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788764, Length=212, Percent_Identity=26.8867924528302, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30732; Mature: 30732

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLPEVRSVWRRVILTVGYTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYTQLFNDSA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCH
MISAVGLSLTIAAASATMAVVLGTIAAVVMVRFGRFRGSTGFAFMLTAPLVMPDVITGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LLLLFVAMGHAIGWPAERGMFTIWLAHVTFCTAYVTVVISSRLRELDRSIEEAAMDLGAT
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLKVFFVITVPMIAPALISGWLLAFTLSLDDLVIASFVAGPGATTLPMLVFSSVRMGVNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
EINALATLILLVVGVIGLFAWWIMSRAEKQRLRELRKAARS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNNLPEVRSVWRRVILTVGYTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYTQLFNDSA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCH
MISAVGLSLTIAAASATMAVVLGTIAAVVMVRFGRFRGSTGFAFMLTAPLVMPDVITGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LLLLFVAMGHAIGWPAERGMFTIWLAHVTFCTAYVTVVISSRLRELDRSIEEAAMDLGAT
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLKVFFVITVPMIAPALISGWLLAFTLSLDDLVIASFVAGPGATTLPMLVFSSVRMGVNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
EINALATLILLVVGVIGLFAWWIMSRAEKQRLRELRKAARS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]