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The map label for this gene is braD [H]

Identifier: 218928351

GI number: 218928351

Start: 1337040

End: 1338641

Strand: Direct

Name: braD [H]

Synonym: YPO1188

Alternate gene names: 218928351

Gene position: 1337040-1338641 (Clockwise)

Preceding gene: 218928350

Following gene: 218928352

Centisome position: 28.73

GC content: 50.0

Gene sequence:

>1602_bases
ATGAAATCGTTAATTCCTGTTTGGAATAGGGCAATCAATTATGTGTTCAGGCCGATGATAGGTCTGTTACTGGGCTTATC
GATGTCGGCAATGGCAGGGCCAGCACAGGATTTTGTTGCGGCGAATCGTGCACAGCAGGCCACATTATTACAACAGTGGG
CAACGGAACCTGATGCTAGCCGATTACCTCTTTTGCAGGCGCTAAAACAAGAAACAGTAGTGGTAGACAGTGCCGGCCAA
GCGTTTATTAAAAATAATCAAAGTATTAATTCGCTGGAAGGCCAATCGCAACCGACCGGTACGCTGAAAAAAATCTGGCT
CAACAACCGGTTACGTATCTTGATCGCCAATGCCCTGGCGGCACAACGCTTAGTCAGTGACGATGAAACCGTGCGTTTAC
AGGCGGCAGTTGAATTACAACGGCAGGCACAGAGCGATCAACTACCGCTGCTGAATCAACGCCTGAAGTTGGAAACGGAC
AGTCAGGTCAAAGAGGCATTGAGCATTGCACTCGCCAATTTACAACTGACGGACAGTAATCCGCAGGTGCGTCTGAATGC
CGTGCAATTGTTGGGGCAATCAGGTTCACCGCAAACTCAATCACGGCTGCAAAGTTTAACCGATACGCAGGTTGAACCGA
ATGAACAGGTCCGAGCCGCAGCAGCCACCAGTTTGAAACAGATACAACATCGGTTGATGATTGGGGAGGTTATTGGGCAG
GTATTTAGCGGACTATCATTGGGTTCAATCTTACTGCTGGCGGCATTGGGGTTAGCTATCACCTATGGCTTACTGGGGGT
GATCAATATGGCGCATGGTGAAATGCTGATGTTGGGAGCCTATTCCGCCTATCTGGTGCAATCGCTATTCCAACAATTTG
CCCCACACTGGCTGGCACTTTATCCATTAATCGCTCTACCGGTTGCGTTCTTTATTACTGCGGGCATTGGTATGGCGTTG
GAACGCACGGTGATTCGGCATCTGTATGGCCGCCCGTTGGAAACACTGCTAGCGACGTGGGGGATCAGCCTGATGTTGAT
TCAGGGGATCCGCATGTTGTTTGGCCCACAGAATTTGGAAGTTGCTAACCCTGCATGGCTGTCCGGTGGGGTTCAGGTTT
TACCTAATCTGGTTTTACCGTGGAATCGGATCGCGGTGATTGCATTTGTCATTATTGTTTTGCTACTCACATGGTTGCTG
CTGAATAAAACCCGCCTTGGTCTGCAAGTCCGTGCAGTGACACAAAATCGGGCAATGGCGGCCTGCTGCGGAGTGGCTAC
GGGGCGGGTTGATATGCTGGCATTCGGTTTGGGTTCTGGTATTGCAGGGCTTGGCGGTGTAGCACTCTCGCAATTGGGCA
ATGTCGGCCCGGAATTAGGGCAGGGTTATATCATCGACTCCTTCTTGGTGGTGGTCCTAGGTGGCGTCGGGCAATTAGCT
GGCAGTGTGGTTGCCGCATTAGGTCTGGGGATGTTGAATAAAATTTTGGAACCACAAATTGGCGCAGTATTGGGCAAAAT
CCTGATTTTAGTGCTGATCATTTTATTTATTCAAAAACGACCGCAGGGTCTGTTTGCCCTTAAGGGCAGGGTGAGTGACT
AA

Upstream 100 bases:

>100_bases
GACAGATGATCGCGGTAAACAGGTAATCGCTGTGACGTGCTGATGTCAGCGGGATCTGACGTCAGCTCTTTTTCACCACA
TATGTGTGATGAGTGGGCCT

Downstream 100 bases:

>100_bases
TGAATCAACCACTGACGGTAACCTTAACGCAAAAAGCACCGCTTACCAGCCTGTTATTAGGTATGGTCACGCTGATCGCA
CTGATTGTTCTGCCTTTTTT

Product: putative Branched-chain amino acid transport system, permease component

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MKSLIPVWNRAINYVFRPMIGLLLGLSMSAMAGPAQDFVAANRAQQATLLQQWATEPDASRLPLLQALKQETVVVDSAGQ
AFIKNNQSINSLEGQSQPTGTLKKIWLNNRLRILIANALAAQRLVSDDETVRLQAAVELQRQAQSDQLPLLNQRLKLETD
SQVKEALSIALANLQLTDSNPQVRLNAVQLLGQSGSPQTQSRLQSLTDTQVEPNEQVRAAAATSLKQIQHRLMIGEVIGQ
VFSGLSLGSILLLAALGLAITYGLLGVINMAHGEMLMLGAYSAYLVQSLFQQFAPHWLALYPLIALPVAFFITAGIGMAL
ERTVIRHLYGRPLETLLATWGISLMLIQGIRMLFGPQNLEVANPAWLSGGVQVLPNLVLPWNRIAVIAFVIIVLLLTWLL
LNKTRLGLQVRAVTQNRAMAACCGVATGRVDMLAFGLGSGIAGLGGVALSQLGNVGPELGQGYIIDSFLVVVLGGVGQLA
GSVVAALGLGMLNKILEPQIGAVLGKILILVLIILFIQKRPQGLFALKGRVSD

Sequences:

>Translated_533_residues
MKSLIPVWNRAINYVFRPMIGLLLGLSMSAMAGPAQDFVAANRAQQATLLQQWATEPDASRLPLLQALKQETVVVDSAGQ
AFIKNNQSINSLEGQSQPTGTLKKIWLNNRLRILIANALAAQRLVSDDETVRLQAAVELQRQAQSDQLPLLNQRLKLETD
SQVKEALSIALANLQLTDSNPQVRLNAVQLLGQSGSPQTQSRLQSLTDTQVEPNEQVRAAAATSLKQIQHRLMIGEVIGQ
VFSGLSLGSILLLAALGLAITYGLLGVINMAHGEMLMLGAYSAYLVQSLFQQFAPHWLALYPLIALPVAFFITAGIGMAL
ERTVIRHLYGRPLETLLATWGISLMLIQGIRMLFGPQNLEVANPAWLSGGVQVLPNLVLPWNRIAVIAFVIIVLLLTWLL
LNKTRLGLQVRAVTQNRAMAACCGVATGRVDMLAFGLGSGIAGLGGVALSQLGNVGPELGQGYIIDSFLVVVLGGVGQLA
GSVVAALGLGMLNKILEPQIGAVLGKILILVLIILFIQKRPQGLFALKGRVSD
>Mature_533_residues
MKSLIPVWNRAINYVFRPMIGLLLGLSMSAMAGPAQDFVAANRAQQATLLQQWATEPDASRLPLLQALKQETVVVDSAGQ
AFIKNNQSINSLEGQSQPTGTLKKIWLNNRLRILIANALAAQRLVSDDETVRLQAAVELQRQAQSDQLPLLNQRLKLETD
SQVKEALSIALANLQLTDSNPQVRLNAVQLLGQSGSPQTQSRLQSLTDTQVEPNEQVRAAAATSLKQIQHRLMIGEVIGQ
VFSGLSLGSILLLAALGLAITYGLLGVINMAHGEMLMLGAYSAYLVQSLFQQFAPHWLALYPLIALPVAFFITAGIGMAL
ERTVIRHLYGRPLETLLATWGISLMLIQGIRMLFGPQNLEVANPAWLSGGVQVLPNLVLPWNRIAVIAFVIIVLLLTWLL
LNKTRLGLQVRAVTQNRAMAACCGVATGRVDMLAFGLGSGIAGLGGVALSQLGNVGPELGQGYIIDSFLVVVLGGVGQLA
GSVVAALGLGMLNKILEPQIGAVLGKILILVLIILFIQKRPQGLFALKGRVSD

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=294, Percent_Identity=27.2108843537415, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 57222; Mature: 57222

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLIPVWNRAINYVFRPMIGLLLGLSMSAMAGPAQDFVAANRAQQATLLQQWATEPDAS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
RLPLLQALKQETVVVDSAGQAFIKNNQSINSLEGQSQPTGTLKKIWLNNRLRILIANALA
CCHHHHHHHHHHEEEECCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCHHEEHHHHHH
AQRLVSDDETVRLQAAVELQRQAQSDQLPLLNQRLKLETDSQVKEALSIALANLQLTDSN
HHHHHCCCCCEEHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHEEEECCC
PQVRLNAVQLLGQSGSPQTQSRLQSLTDTQVEPNEQVRAAAATSLKQIQHRLMIGEVIGQ
CCEEEHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VFSGLSLGSILLLAALGLAITYGLLGVINMAHGEMLMLGAYSAYLVQSLFQQFAPHWLAL
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCHHHHHH
YPLIALPVAFFITAGIGMALERTVIRHLYGRPLETLLATWGISLMLIQGIRMLFGPQNLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VANPAWLSGGVQVLPNLVLPWNRIAVIAFVIIVLLLTWLLLNKTRLGLQVRAVTQNRAMA
CCCCHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCEEEEEEHHCCHHH
ACCGVATGRVDMLAFGLGSGIAGLGGVALSQLGNVGPELGQGYIIDSFLVVVLGGVGQLA
HHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHCCHHHHH
GSVVAALGLGMLNKILEPQIGAVLGKILILVLIILFIQKRPQGLFALKGRVSD
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
>Mature Secondary Structure
MKSLIPVWNRAINYVFRPMIGLLLGLSMSAMAGPAQDFVAANRAQQATLLQQWATEPDAS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
RLPLLQALKQETVVVDSAGQAFIKNNQSINSLEGQSQPTGTLKKIWLNNRLRILIANALA
CCHHHHHHHHHHEEEECCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCHHEEHHHHHH
AQRLVSDDETVRLQAAVELQRQAQSDQLPLLNQRLKLETDSQVKEALSIALANLQLTDSN
HHHHHCCCCCEEHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHEEEECCC
PQVRLNAVQLLGQSGSPQTQSRLQSLTDTQVEPNEQVRAAAATSLKQIQHRLMIGEVIGQ
CCEEEHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VFSGLSLGSILLLAALGLAITYGLLGVINMAHGEMLMLGAYSAYLVQSLFQQFAPHWLAL
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCHHHHHH
YPLIALPVAFFITAGIGMALERTVIRHLYGRPLETLLATWGISLMLIQGIRMLFGPQNLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VANPAWLSGGVQVLPNLVLPWNRIAVIAFVIIVLLLTWLLLNKTRLGLQVRAVTQNRAMA
CCCCHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCEEEEEEHHCCHHH
ACCGVATGRVDMLAFGLGSGIAGLGGVALSQLGNVGPELGQGYIIDSFLVVVLGGVGQLA
HHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHCCHHHHH
GSVVAALGLGMLNKILEPQIGAVLGKILILVLIILFIQKRPQGLFALKGRVSD
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]