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The map label for this gene is mchE [H]

Identifier: 218927314

GI number: 218927314

Start: 108358

End: 109593

Strand: Reverse

Name: mchE [H]

Synonym: YPO0099

Alternate gene names: 218927314

Gene position: 109593-108358 (Counterclockwise)

Preceding gene: 218927318

Following gene: 218927313

Centisome position: 2.35

GC content: 38.83

Gene sequence:

>1236_bases
TTGGGGAAGGCGCTGTTAACTTCGGGTTATTCCACAACGTTCATAGCTGCTTTATGTGCTATCTTTTTGGTGTTATTAAT
TACTCTTATCATTTATGGAACATACACCCGTAGAATAAACGTTAATGGTGAGGTTATTTCTCAGCCTCACCCCATAAACA
TTTTTTCTCCCCAACAAGGATTCATTACCAAAAAATGGGTGGAAGTGGGTGATATTGTCCGTAAAGGTCAGCACTTATAT
CAAATTGATGTCAGTCGAACGACCTTTTCAGGTAATGTTAGCCTTAACTCTTTAGAAGCAATAAATAACCAACTTTCTCA
AATTGATAGCATCATCAATAATACACAGAAAAATAAAGAACTTACGCTACTTAATTTACGTCAGCAGCTCGCACAATACC
AAAAGGCACATAAGAAATCCCAAGAGCTGGTCGATAACGCAGGCAAAGGAATGGATGATATGCGGCGTACAATGGCCAGT
TATGGTACTTACCAACGTCAGGGTCTAATTACTAAAGATCAATTAACCAATCAGCGTTCCCTCTTTTATCAACAACAGAA
TGCCTTTCAAAGCCTGAATACTCAACTGATACAAGAAAGCTTACAGATAGCAAAACTTGAAAGTGAAATCTCTACGCGAG
CATCAGATTTTGATAATGATATCTCTCAGTATTTATTTCAAAAAGGTGATTTAAAGCGTCAATTGGCTGAAGTTGATGCG
AGTGGCATGTTACTCATTAATTCGCCAAGCGATGGCAAGATTGAAAATATGAGTGTGACCCAAGGACAGATGGTCAACGT
AAATGACAGTTTGGTCCAATTGACGCCTTCTGATAATCCCTATTATTGTCTTGTGCTTTGGGTACCAAACAATAGCGTCC
CTTATATAAATACTGGAGACAAAGTCAATATACGCTATGACGCTTTCCCCTTTGAAAAGTTTGGTCAATTTCCTGGGCGT
ATAATCTCTATCTCTAATGTCCCCGTTTCACAACAAGAAATAGCCTCATACAATATTGCACCGCGGCTCCCGAACGGTGG
ACTCATTGAGCCTTACTATAAAGTCATCGTGGCACTAGACGATATTCATTTTCGTTATCAGTCTAAACCTTTAATGCTAT
CCAATGGCCTTAAAGCTAATGTCACGTTATTCCTTGAAAAAAGGCCTCTTTATCAATGGATGCTATCTCCATTTTATGAC
ATCAAGAAAAGTGTAACGGGGCCTGTAAATGAATGA

Upstream 100 bases:

>100_bases
TGTGATATGTTGATTTGAAATGATGGGTATAACGGAGCGAGGAATGAGCAGCAATCAGATATTCCGCCGGGACGCGGTAG
AGTATATTAGAACAAAATGG

Downstream 100 bases:

>100_bases
TACGCATTTTAACGAGATAAAAAACCGTCTGAATTTTAGCTTTCGTCGTAAAGTCCCACAGGTGCTTCAAACCGAAGCAG
CAGAATGCGGACTTGCCTGT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MGKALLTSGYSTTFIAALCAIFLVLLITLIIYGTYTRRINVNGEVISQPHPINIFSPQQGFITKKWVEVGDIVRKGQHLY
QIDVSRTTFSGNVSLNSLEAINNQLSQIDSIINNTQKNKELTLLNLRQQLAQYQKAHKKSQELVDNAGKGMDDMRRTMAS
YGTYQRQGLITKDQLTNQRSLFYQQQNAFQSLNTQLIQESLQIAKLESEISTRASDFDNDISQYLFQKGDLKRQLAEVDA
SGMLLINSPSDGKIENMSVTQGQMVNVNDSLVQLTPSDNPYYCLVLWVPNNSVPYINTGDKVNIRYDAFPFEKFGQFPGR
IISISNVPVSQQEIASYNIAPRLPNGGLIEPYYKVIVALDDIHFRYQSKPLMLSNGLKANVTLFLEKRPLYQWMLSPFYD
IKKSVTGPVNE

Sequences:

>Translated_411_residues
MGKALLTSGYSTTFIAALCAIFLVLLITLIIYGTYTRRINVNGEVISQPHPINIFSPQQGFITKKWVEVGDIVRKGQHLY
QIDVSRTTFSGNVSLNSLEAINNQLSQIDSIINNTQKNKELTLLNLRQQLAQYQKAHKKSQELVDNAGKGMDDMRRTMAS
YGTYQRQGLITKDQLTNQRSLFYQQQNAFQSLNTQLIQESLQIAKLESEISTRASDFDNDISQYLFQKGDLKRQLAEVDA
SGMLLINSPSDGKIENMSVTQGQMVNVNDSLVQLTPSDNPYYCLVLWVPNNSVPYINTGDKVNIRYDAFPFEKFGQFPGR
IISISNVPVSQQEIASYNIAPRLPNGGLIEPYYKVIVALDDIHFRYQSKPLMLSNGLKANVTLFLEKRPLYQWMLSPFYD
IKKSVTGPVNE
>Mature_410_residues
GKALLTSGYSTTFIAALCAIFLVLLITLIIYGTYTRRINVNGEVISQPHPINIFSPQQGFITKKWVEVGDIVRKGQHLYQ
IDVSRTTFSGNVSLNSLEAINNQLSQIDSIINNTQKNKELTLLNLRQQLAQYQKAHKKSQELVDNAGKGMDDMRRTMASY
GTYQRQGLITKDQLTNQRSLFYQQQNAFQSLNTQLIQESLQIAKLESEISTRASDFDNDISQYLFQKGDLKRQLAEVDAS
GMLLINSPSDGKIENMSVTQGQMVNVNDSLVQLTPSDNPYYCLVLWVPNNSVPYINTGDKVNIRYDAFPFEKFGQFPGRI
ISISNVPVSQQEIASYNIAPRLPNGGLIEPYYKVIVALDDIHFRYQSKPLMLSNGLKANVTLFLEKRPLYQWMLSPFYDI
KKSVTGPVNE

Specific function: Probably involved, in conjunction with mchF, in the secretion of microcin H47 [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003997
- InterPro:   IPR006143
- InterPro:   IPR006144
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 46463; Mature: 46331

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS00543 HLYD_FAMILY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKALLTSGYSTTFIAALCAIFLVLLITLIIYGTYTRRINVNGEVISQPHPINIFSPQQG
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEECCCCCCEEECCCCC
FITKKWVEVGDIVRKGQHLYQIDVSRTTFSGNVSLNSLEAINNQLSQIDSIINNTQKNKE
CHHHHHHHHHHHHHCCCEEEEEEECEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
LTLLNLRQQLAQYQKAHKKSQELVDNAGKGMDDMRRTMASYGTYQRQGLITKDQLTNQRS
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHCCCCCHHHHCCHHH
LFYQQQNAFQSLNTQLIQESLQIAKLESEISTRASDFDNDISQYLFQKGDLKRQLAEVDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
SGMLLINSPSDGKIENMSVTQGQMVNVNDSLVQLTPSDNPYYCLVLWVPNNSVPYINTGD
CCEEEEECCCCCCEEEEEECCCEEEECCCCEEEECCCCCCEEEEEEEECCCCCCEEECCC
KVNIRYDAFPFEKFGQFPGRIISISNVPVSQQEIASYNIAPRLPNGGLIEPYYKVIVALD
EEEEEEECCCHHHHCCCCCEEEEECCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHEEEE
DIHFRYQSKPLMLSNGLKANVTLFLEKRPLYQWMLSPFYDIKKSVTGPVNE
CCEEEECCCCEEEECCCCEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GKALLTSGYSTTFIAALCAIFLVLLITLIIYGTYTRRINVNGEVISQPHPINIFSPQQG
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEECCCCCCEEECCCCC
FITKKWVEVGDIVRKGQHLYQIDVSRTTFSGNVSLNSLEAINNQLSQIDSIINNTQKNKE
CHHHHHHHHHHHHHCCCEEEEEEECEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
LTLLNLRQQLAQYQKAHKKSQELVDNAGKGMDDMRRTMASYGTYQRQGLITKDQLTNQRS
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHCCCCCHHHHCCHHH
LFYQQQNAFQSLNTQLIQESLQIAKLESEISTRASDFDNDISQYLFQKGDLKRQLAEVDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
SGMLLINSPSDGKIENMSVTQGQMVNVNDSLVQLTPSDNPYYCLVLWVPNNSVPYINTGD
CCEEEEECCCCCCEEEEEECCCEEEECCCCEEEECCCCCCEEEEEEEECCCCCCEEECCC
KVNIRYDAFPFEKFGQFPGRIISISNVPVSQQEIASYNIAPRLPNGGLIEPYYKVIVALD
EEEEEEECCCHHHHCCCCCEEEEECCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHEEEE
DIHFRYQSKPLMLSNGLKANVTLFLEKRPLYQWMLSPFYDIKKSVTGPVNE
CCEEEECCCCEEEECCCCEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11181394 [H]