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The map label for this gene is lpdA [C]

Identifier: 15922579

GI number: 15922579

Start: 2255086

End: 2256408

Strand: Direct

Name: lpdA [C]

Synonym: ST2247

Alternate gene names: 15922579

Gene position: 2255086-2256408 (Clockwise)

Preceding gene: 15922575

Following gene: 15922580

Centisome position: 83.68

GC content: 35.45

Gene sequence:

>1323_bases
ATGGAAACTTTAGTAGCAGTAGGTGGCGGAGCTGCTGGAATGAGTGCTTCTTCAAGAGCTAGGAGATTAAAGCCAGACAT
GGAAATTGTTGTATTTGAATCAACAAAAATGGTTAGTCATGCACCTTGTGGAATTCCTTATTTCGTTGAAGGATTATTTA
ATGACGAGAATCTTTTTATGACTTATACTCCACAATTTTTTGAAGAAAAGAGAAAAATTCATGTAAAAACTAATACGAAA
GTAACAGAAATTGATTTTGATTCCCGGATAGTATATGGGGAATCTAGAGAAGGAAAAATCAAAGCTGAATATGATTATTT
AGTTATTAGTACGGGAGCTATACCTAGAAAAATTCCAGTTGAAGACGGAAATGATAGGATTTTTTATGCACATCATCCGG
CTAATGCAGTAGAATTAAGAGAGAGACTTTGGTCTTTAAATACTATCGCAATAGTAGGAGGTGGAATATTAGGTATAGAA
ATGGCGGAGGCACTAACTCATATAGGCAAAAAGGTAATTTTGATTCACAGGAGTAAGTATTTGCTAAATAAGACTATAGA
CACTGAACTGGGAAATATAATAACTCAAAGAGTTTCTAAAGATGCAGAAGTTAGACTCAATGAGAGCGTTGAGACTATAA
AGGAAGGAGGTAGGTTAATAGTCACAGATAAGGGAAAGTACCAAGTAGATGGCACTATAATAGCAATAGGAGTTGCACCA
AATGTTGAACTTGTAAAAGATAAAATAAAATTAGGAGAGACTGGAGCTATAAAGGTTGATGATCACATGAGGACTAACTA
TAGGGAAGTTTATTCAGCTGGTGATAACACTGAATCAGTAAATATAATAACTAAAAAACCCGCATGGGTTCCTTTTGCAC
CAGTAGCTAATAAGATGGGTTATGTGGCTGGAAGTAATATAGGCGGTCATGAAATGAGATTCCCAGGAGTTGTGAACACC
CAAATTACTAAGTACAAAGAATTTTATATAGGACGAGTGGGTTTACAAGAAGATGAGGCAAGGCTTCACGGATTTAAACC
TATATCAGCAACTATAAGTGGGAAGACTAGAGCAAGATATTATCCTGACGCTAAAGATATTCATGTAAAAATTATAGCAG
ATGAAAACACTAAGAGAATTTTAGGTGCTCAAATCGTTGGTGGTGAAGAAGTATTAGGACGAATAGATATGATGGCTGCA
GCGATAATGAAAGGCTTTACCATAGAAGATACATTCTTCATTGAGATGGGTTACTTACCAGCAATTAGTAGAGTTTGGGA
TCCAGTAATTGTAGCAATTAGACAACTTATGAAAGATGAATAA

Upstream 100 bases:

>100_bases
GTTTCAAGTTTTTTTATTAAATCCTCAAATAGATTTCTTGTTGTCTCCATAAAAGAATCTTATTAATACTATATAAAAAG
GTCTTCTCTTAATTTAATGT

Downstream 100 bases:

>100_bases
GAAGAGGTAATACCCATTGGTATTACGCCATATTTAAATATTTCTCTCGATATTTTTATATGAAAGATGACTGAAAATAA
GGGATTATTTGTATTACTTG

Product: NADH oxidase

Products: NA

Alternate protein names: CoA-disulfide reductase; CoADR [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
METLVAVGGGAAGMSASSRARRLKPDMEIVVFESTKMVSHAPCGIPYFVEGLFNDENLFMTYTPQFFEEKRKIHVKTNTK
VTEIDFDSRIVYGESREGKIKAEYDYLVISTGAIPRKIPVEDGNDRIFYAHHPANAVELRERLWSLNTIAIVGGGILGIE
MAEALTHIGKKVILIHRSKYLLNKTIDTELGNIITQRVSKDAEVRLNESVETIKEGGRLIVTDKGKYQVDGTIIAIGVAP
NVELVKDKIKLGETGAIKVDDHMRTNYREVYSAGDNTESVNIITKKPAWVPFAPVANKMGYVAGSNIGGHEMRFPGVVNT
QITKYKEFYIGRVGLQEDEARLHGFKPISATISGKTRARYYPDAKDIHVKIIADENTKRILGAQIVGGEEVLGRIDMMAA
AIMKGFTIEDTFFIEMGYLPAISRVWDPVIVAIRQLMKDE

Sequences:

>Translated_440_residues
METLVAVGGGAAGMSASSRARRLKPDMEIVVFESTKMVSHAPCGIPYFVEGLFNDENLFMTYTPQFFEEKRKIHVKTNTK
VTEIDFDSRIVYGESREGKIKAEYDYLVISTGAIPRKIPVEDGNDRIFYAHHPANAVELRERLWSLNTIAIVGGGILGIE
MAEALTHIGKKVILIHRSKYLLNKTIDTELGNIITQRVSKDAEVRLNESVETIKEGGRLIVTDKGKYQVDGTIIAIGVAP
NVELVKDKIKLGETGAIKVDDHMRTNYREVYSAGDNTESVNIITKKPAWVPFAPVANKMGYVAGSNIGGHEMRFPGVVNT
QITKYKEFYIGRVGLQEDEARLHGFKPISATISGKTRARYYPDAKDIHVKIIADENTKRILGAQIVGGEEVLGRIDMMAA
AIMKGFTIEDTFFIEMGYLPAISRVWDPVIVAIRQLMKDE
>Mature_440_residues
METLVAVGGGAAGMSASSRARRLKPDMEIVVFESTKMVSHAPCGIPYFVEGLFNDENLFMTYTPQFFEEKRKIHVKTNTK
VTEIDFDSRIVYGESREGKIKAEYDYLVISTGAIPRKIPVEDGNDRIFYAHHPANAVELRERLWSLNTIAIVGGGILGIE
MAEALTHIGKKVILIHRSKYLLNKTIDTELGNIITQRVSKDAEVRLNESVETIKEGGRLIVTDKGKYQVDGTIIAIGVAP
NVELVKDKIKLGETGAIKVDDHMRTNYREVYSAGDNTESVNIITKKPAWVPFAPVANKMGYVAGSNIGGHEMRFPGVVNT
QITKYKEFYIGRVGLQEDEARLHGFKPISATISGKTRARYYPDAKDIHVKIIADENTKRILGAQIVGGEEVLGRIDMMAA
AIMKGFTIEDTFFIEMGYLPAISRVWDPVIVAIRQLMKDE

Specific function: Lipoamide Dehydrogenase Is A Component Of The Glycine Cleavage System As Well As Of The Alpha-Ketoacid Dehydrogenase Complexes. [C]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786307, Length=333, Percent_Identity=28.5285285285285, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788892, Length=283, Percent_Identity=25.7950530035336, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1789065, Length=216, Percent_Identity=25.9259259259259, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017758
- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.14 [H]

Molecular weight: Translated: 48926; Mature: 48926

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METLVAVGGGAAGMSASSRARRLKPDMEIVVFESTKMVSHAPCGIPYFVEGLFNDENLFM
CCEEEEECCCCCCCCCHHHHHHCCCCCEEEEEECCCEECCCCCCCCHHHHEEECCCCEEE
TYTPQFFEEKRKIHVKTNTKVTEIDFDSRIVYGESREGKIKAEYDYLVISTGAIPRKIPV
EECHHHHCCCCEEEEEECCEEEEEECCCEEEECCCCCCCEEEEEEEEEEECCCCCCCCCC
EDGNDRIFYAHHPANAVELRERLWSLNTIAIVGGGILGIEMAEALTHIGKKVILIHRSKY
CCCCCEEEEEECCCCHHHHHHHHHCCCEEEEECCCEEHHHHHHHHHHCCCEEEEEECCHH
LLNKTIDTELGNIITQRVSKDAEVRLNESVETIKEGGRLIVTDKGKYQVDGTIIAIGVAP
HHHCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHCCCEEEEECCCCEEECCEEEEEEECC
NVELVKDKIKLGETGAIKVDDHMRTNYREVYSAGDNTESVNIITKKPAWVPFAPVANKMG
CHHHHHHHHHCCCCCEEEECCCHHCCHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHCCC
YVAGSNIGGHEMRFPGVVNTQITKYKEFYIGRVGLQEDEARLHGFKPISATISGKTRARY
EEECCCCCCCCEECCCCCCCHHHHEEHHEEEECCCCCCHHHHCCCCCEEEEECCCEEEEE
YPDAKDIHVKIIADENTKRILGAQIVGGEEVLGRIDMMAAAIMKGFTIEDTFFIEMGYLP
CCCCCCEEEEEEECCCCCEEEEEEEECHHHHHHHHHHHHHHHHCCCEECCEEEEEECCCH
AISRVWDPVIVAIRQLMKDE
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
METLVAVGGGAAGMSASSRARRLKPDMEIVVFESTKMVSHAPCGIPYFVEGLFNDENLFM
CCEEEEECCCCCCCCCHHHHHHCCCCCEEEEEECCCEECCCCCCCCHHHHEEECCCCEEE
TYTPQFFEEKRKIHVKTNTKVTEIDFDSRIVYGESREGKIKAEYDYLVISTGAIPRKIPV
EECHHHHCCCCEEEEEECCEEEEEECCCEEEECCCCCCCEEEEEEEEEEECCCCCCCCCC
EDGNDRIFYAHHPANAVELRERLWSLNTIAIVGGGILGIEMAEALTHIGKKVILIHRSKY
CCCCCEEEEEECCCCHHHHHHHHHCCCEEEEECCCEEHHHHHHHHHHCCCEEEEEECCHH
LLNKTIDTELGNIITQRVSKDAEVRLNESVETIKEGGRLIVTDKGKYQVDGTIIAIGVAP
HHHCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHCCCEEEEECCCCEEECCEEEEEEECC
NVELVKDKIKLGETGAIKVDDHMRTNYREVYSAGDNTESVNIITKKPAWVPFAPVANKMG
CHHHHHHHHHCCCCCEEEECCCHHCCHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHCCC
YVAGSNIGGHEMRFPGVVNTQITKYKEFYIGRVGLQEDEARLHGFKPISATISGKTRARY
EEECCCCCCCCEECCCCCCCHHHHEEHHEEEECCCCCCHHHHCCCCCEEEEECCCEEEEE
YPDAKDIHVKIIADENTKRILGAQIVGGEEVLGRIDMMAAAIMKGFTIEDTFFIEMGYLP
CCCCCCEEEEEEECCCCCEEEEEEEECHHHHHHHHHHHHHHHHCCCEECCEEEEEECCCH
AISRVWDPVIVAIRQLMKDE
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA