Click here to switch to the map view.

The map label for this gene is nuoN2 [H]

Identifier: 15922552

GI number: 15922552

Start: 2231874

End: 2233262

Strand: Direct

Name: nuoN2 [H]

Synonym: ST2223

Alternate gene names: 15922552

Gene position: 2231874-2233262 (Clockwise)

Preceding gene: 15922551

Following gene: 15922553

Centisome position: 82.82

GC content: 32.83

Gene sequence:

>1389_bases
ATGATACTTCAATATATTCCCATAGTTCTTCCTTCAATTTTAATAGTATTTTCATCTATAGCTGTTCTATATATTGATAA
TGGTACTAGAAGCAGATATATTATAGCAGTCGATTTATCTATTGGTACTCTGCTGGTTTCTTTTTTAACTCTAATAATTT
TTTACGGGTTAGGATATTATGGCTACTCTCTTTTTTCTTCTACTCTTTACTTATCTAATTTCGGATATTTTATTGCAGTA
TCCGCAATATTAGCTACAATAATAGTTATTTATGGAGGAATGGAAAGCTTAGAGCCTATTAAGACTAGATCCTCTTTCTT
ATCCTTAGCGATGTTAACAGATTTAGGCGTAATTTATTTAGGTTTCGCGTATAGTGTAATTACCATACTAGCATCATGGG
GTATAGCATCTGCAGCAACTTATGTAATTGCAATGATTAGGAAGGACTACTCTTCGACTATTGCTGGAGTAAAATACTTA
ATTATGGGGTTACTCTCAAGCTCTCTAATGGTTTTAGGTTTCGCATTCTTCATTTTAGGAATTGGTTCTCTATCATTAGA
TGCTACTATAAACTACCAAACTTTAGTAATATTAGGTATTATGTTTCTTTCAATAGCTTTCCTCTTTAAAGTAGGGGCTT
TTCCATTTCAAGCATGGCTTCCAGATGTATACTCTATGTCAGACAGAATATCTGTAGCTTTTGTATCAAGTGTAGGTAAA
ATAGTAGGTATTGCACCTCTCTTTGATATAATCTATTTCTTAAAACCATCTGGTATAGTAGGATTCTCCATATTCGTAAT
TTTTGCTTTGATTACAGTAATGAGCCTAATATTTGGAAATATAGTAGCTTTCTCGAGGCAAGATTTTGCGTCAATGTTAG
CATATAGTAGTATTACCCAAGTGGGGTTTATGTTAATTGCAATAACTATGCTACCTTATAATCCAACTGTTTCTACTAGT
GGATTAATGGTTTATTTATTAGCTTACTCAATAGCTCAAGCTGGTTTATTTATTGCTTTATCCCATATAGAGAAAGTTAG
CGGAACTAGTTATATTGAAGGTTTTAGAGGAATGAGTTCTAGTGATAAAGCTTTAGCATTCGCGCTTTCAATATTACTCC
TAAGCTTATTAGGTATACCGCCAATATTAGGATTTTGGGCTAAATTATTTGTTCTAGAAGCATCATTCTCACAGCCGTGG
TTAACTGTTATTGGGTTTTTGAATAGTGCTGTATCAGCTGGATACTATATACCGCCAATTAGAGAAATTTTTAGAGAAGG
AGAGTTTAGGAATGTAAATTCTACAGAAAGAGATGCAGCAATTATAGCATCAATTTTAAGTATAGCTTTGGGTATAGTAG
CACCATTGATAGTAGGTGTTATATCTTGA

Upstream 100 bases:

>100_bases
GACTGAAACAGGGATTTTGCGTGATTACATTTTTATGTATTTAATAGGCCTTGTAATTCTTGCTTTTATCATAATCTTTT
TCATTATGGGGGTGTAAAAT

Downstream 100 bases:

>100_bases
TTAAGGTTGCAATTTTTGGCGTAGGAAATGTTGCAAGTGCTCTTTTACAAGGTCTAGAATGGCTAAAGGAAGGAAAAAAT
TTACCAGGATTGTTAGATCT

Product: NADH dehydrogenase subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N 2; NDH-1 subunit N 2 [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MILQYIPIVLPSILIVFSSIAVLYIDNGTRSRYIIAVDLSIGTLLVSFLTLIIFYGLGYYGYSLFSSTLYLSNFGYFIAV
SAILATIIVIYGGMESLEPIKTRSSFLSLAMLTDLGVIYLGFAYSVITILASWGIASAATYVIAMIRKDYSSTIAGVKYL
IMGLLSSSLMVLGFAFFILGIGSLSLDATINYQTLVILGIMFLSIAFLFKVGAFPFQAWLPDVYSMSDRISVAFVSSVGK
IVGIAPLFDIIYFLKPSGIVGFSIFVIFALITVMSLIFGNIVAFSRQDFASMLAYSSITQVGFMLIAITMLPYNPTVSTS
GLMVYLLAYSIAQAGLFIALSHIEKVSGTSYIEGFRGMSSSDKALAFALSILLLSLLGIPPILGFWAKLFVLEASFSQPW
LTVIGFLNSAVSAGYYIPPIREIFREGEFRNVNSTERDAAIIASILSIALGIVAPLIVGVIS

Sequences:

>Translated_462_residues
MILQYIPIVLPSILIVFSSIAVLYIDNGTRSRYIIAVDLSIGTLLVSFLTLIIFYGLGYYGYSLFSSTLYLSNFGYFIAV
SAILATIIVIYGGMESLEPIKTRSSFLSLAMLTDLGVIYLGFAYSVITILASWGIASAATYVIAMIRKDYSSTIAGVKYL
IMGLLSSSLMVLGFAFFILGIGSLSLDATINYQTLVILGIMFLSIAFLFKVGAFPFQAWLPDVYSMSDRISVAFVSSVGK
IVGIAPLFDIIYFLKPSGIVGFSIFVIFALITVMSLIFGNIVAFSRQDFASMLAYSSITQVGFMLIAITMLPYNPTVSTS
GLMVYLLAYSIAQAGLFIALSHIEKVSGTSYIEGFRGMSSSDKALAFALSILLLSLLGIPPILGFWAKLFVLEASFSQPW
LTVIGFLNSAVSAGYYIPPIREIFREGEFRNVNSTERDAAIIASILSIALGIVAPLIVGVIS
>Mature_462_residues
MILQYIPIVLPSILIVFSSIAVLYIDNGTRSRYIIAVDLSIGTLLVSFLTLIIFYGLGYYGYSLFSSTLYLSNFGYFIAV
SAILATIIVIYGGMESLEPIKTRSSFLSLAMLTDLGVIYLGFAYSVITILASWGIASAATYVIAMIRKDYSSTIAGVKYL
IMGLLSSSLMVLGFAFFILGIGSLSLDATINYQTLVILGIMFLSIAFLFKVGAFPFQAWLPDVYSMSDRISVAFVSSVGK
IVGIAPLFDIIYFLKPSGIVGFSIFVIFALITVMSLIFGNIVAFSRQDFASMLAYSSITQVGFMLIAITMLPYNPTVSTS
GLMVYLLAYSIAQAGLFIALSHIEKVSGTSYIEGFRGMSSSDKALAFALSILLLSLLGIPPILGFWAKLFVLEASFSQPW
LTVIGFLNSAVSAGYYIPPIREIFREGEFRNVNSTERDAAIIASILSIALGIVAPLIVGVIS

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Escherichia coli, GI145693160, Length=401, Percent_Identity=26.9326683291771, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788829, Length=211, Percent_Identity=25.5924170616114, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788827, Length=276, Percent_Identity=25.3623188405797, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 50003; Mature: 50003

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILQYIPIVLPSILIVFSSIAVLYIDNGTRSRYIIAVDLSIGTLLVSFLTLIIFYGLGYY
CCHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHH
GYSLFSSTLYLSNFGYFIAVSAILATIIVIYGGMESLEPIKTRSSFLSLAMLTDLGVIYL
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHH
GFAYSVITILASWGIASAATYVIAMIRKDYSSTIAGVKYLIMGLLSSSLMVLGFAFFILG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGSLSLDATINYQTLVILGIMFLSIAFLFKVGAFPFQAWLPDVYSMSDRISVAFVSSVGK
CCCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHCCCCHHHHHHHHHHHH
IVGIAPLFDIIYFLKPSGIVGFSIFVIFALITVMSLIFGNIVAFSRQDFASMLAYSSITQ
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGFMLIAITMLPYNPTVSTSGLMVYLLAYSIAQAGLFIALSHIEKVSGTSYIEGFRGMSS
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCC
SDKALAFALSILLLSLLGIPPILGFWAKLFVLEASFSQPWLTVIGFLNSAVSAGYYIPPI
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCH
REIFREGEFRNVNSTERDAAIIASILSIALGIVAPLIVGVIS
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MILQYIPIVLPSILIVFSSIAVLYIDNGTRSRYIIAVDLSIGTLLVSFLTLIIFYGLGYY
CCHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHH
GYSLFSSTLYLSNFGYFIAVSAILATIIVIYGGMESLEPIKTRSSFLSLAMLTDLGVIYL
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHH
GFAYSVITILASWGIASAATYVIAMIRKDYSSTIAGVKYLIMGLLSSSLMVLGFAFFILG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGSLSLDATINYQTLVILGIMFLSIAFLFKVGAFPFQAWLPDVYSMSDRISVAFVSSVGK
CCCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHCCCCHHHHHHHHHHHH
IVGIAPLFDIIYFLKPSGIVGFSIFVIFALITVMSLIFGNIVAFSRQDFASMLAYSSITQ
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGFMLIAITMLPYNPTVSTSGLMVYLLAYSIAQAGLFIALSHIEKVSGTSYIEGFRGMSS
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCC
SDKALAFALSILLLSLLGIPPILGFWAKLFVLEASFSQPWLTVIGFLNSAVSAGYYIPPI
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCH
REIFREGEFRNVNSTERDAAIIASILSIALGIVAPLIVGVIS
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]