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The map label for this gene is lpdA [H]

Identifier: 15922193

GI number: 15922193

Start: 1889225

End: 1890538

Strand: Direct

Name: lpdA [H]

Synonym: ST1879

Alternate gene names: 15922193

Gene position: 1889225-1890538 (Clockwise)

Preceding gene: 15922191

Following gene: 15922194

Centisome position: 70.11

GC content: 32.27

Gene sequence:

>1314_bases
ATGGAATATGATGTTATAATAATAGGCGGAGGAACAGCAGGGTATACTGCTGGCGTCATATTAGGAAGAAGAGGTAAAAA
AATAGCAGTAGTAGAAAAGGAAAAGTTTGGAGGTACTTGTGTAAATTATGGATGTGTTCCAAGTATTTTTTTATCAGATA
TTTCTTTTTTGTATTCACGCCTTTCCGAAATAGGCAATTATAAGGGTATTGAAATTAATTTAAAATTAAATAATTTCTTT
TCTAAAAGAGAAGAAATAATAGACTATTTGAGTACAGCGGGTAAGAGACTGATTGAAAATGCTAGTGGAGAGACAATTGA
AGGAGAAGGTATAATAAGAGATAATCATACAGTTGAGGTCAACGGAAGGCTACTGACTACTAAAGAGATTATTATTGCTA
CTGGTTCCAAACCTAAACCTCCAAGAATAGAAGGTATTGAAAATGCAATAAGCGAAGATCAAGTTGTCAGACTTAATACT
ATTCCTTCATCAATGGTAATTATTGGTGGAGGAGTTGCTGGTACTGAAATTGCTCAAATATTTGCAAAATTAGGAACTAA
AGTTACTCTTTTAACAAGAAGCAAAATATTAAAAGAACTTCATGAAGAAACTAGAAAATTAATGATTGATTCGCTAGAGT
TTGATGGGATAGATATCATAGAGAATTCTAAAATAGAGAAGATATACGGAGAAAAAGTTTATACAAACAAAGGTGTATTT
GATGGAGAAATTGTAGTATATGCCACTGGAAGGGAACCAAATTATCCAAAAGGATTTGAAAAACTAAATGTGGAAATTGG
AAAGGAAGGAATAATAGTTAATGAAAGAATGCAGACTAATATTAAGAACATCTATGCTATTGGCGATATTGTAAATAAGG
AGAAAAAAGTTGCACATGTTGCATATATGGAGGCAATTATTGCCTCCCTTAATGTCTTAGGTACATGTGAATATATGGAT
TATACTGGAACACCTCAAGTAATCTATACAGATCCACAAATAGGTATAGCAGGTGAAAAAGATAAAGCAGTTAAGTTTCT
TAAGTTTCCTTATAATGCTGATACTAGAGCAATAATAAAAGGGTTAAGGGAGGGATTTGCATTAATAGGGATTGATAAGA
ACGGTACTATTGTCTATTCAGAAATAATTGGTGATAATGCTGAGGAGCTCATAAATATAATGGCTTTGGCTATACGCAAG
AGAGTTACAATCAGAGATTTAGCTTTTACAATATTCATTCATCCTTCCTTAAGTGAGATTCTCGTTAATGCGGCAAGAGG
AGAATTTGATTTAGATGTTGACTTATATAAATAA

Upstream 100 bases:

>100_bases
GAATGAAAGCGATGTAGTAGACGGAGTAGAAATTGTAGGAGGATCAACATTAATAGATTTACTCTTCGACGCTGATAGAG
CATTATTCTTCTGACCTAAC

Downstream 100 bases:

>100_bases
TAATGAGAAACAATGTCAAGCCTAGAGGAATACTTAAAAAAGAAGGGATTTCAACTCGTTAATGATGGAAAAACAGAAAA
AATCATAATGGATGACTATG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MEYDVIIIGGGTAGYTAGVILGRRGKKIAVVEKEKFGGTCVNYGCVPSIFLSDISFLYSRLSEIGNYKGIEINLKLNNFF
SKREEIIDYLSTAGKRLIENASGETIEGEGIIRDNHTVEVNGRLLTTKEIIIATGSKPKPPRIEGIENAISEDQVVRLNT
IPSSMVIIGGGVAGTEIAQIFAKLGTKVTLLTRSKILKELHEETRKLMIDSLEFDGIDIIENSKIEKIYGEKVYTNKGVF
DGEIVVYATGREPNYPKGFEKLNVEIGKEGIIVNERMQTNIKNIYAIGDIVNKEKKVAHVAYMEAIIASLNVLGTCEYMD
YTGTPQVIYTDPQIGIAGEKDKAVKFLKFPYNADTRAIIKGLREGFALIGIDKNGTIVYSEIIGDNAEELINIMALAIRK
RVTIRDLAFTIFIHPSLSEILVNAARGEFDLDVDLYK

Sequences:

>Translated_437_residues
MEYDVIIIGGGTAGYTAGVILGRRGKKIAVVEKEKFGGTCVNYGCVPSIFLSDISFLYSRLSEIGNYKGIEINLKLNNFF
SKREEIIDYLSTAGKRLIENASGETIEGEGIIRDNHTVEVNGRLLTTKEIIIATGSKPKPPRIEGIENAISEDQVVRLNT
IPSSMVIIGGGVAGTEIAQIFAKLGTKVTLLTRSKILKELHEETRKLMIDSLEFDGIDIIENSKIEKIYGEKVYTNKGVF
DGEIVVYATGREPNYPKGFEKLNVEIGKEGIIVNERMQTNIKNIYAIGDIVNKEKKVAHVAYMEAIIASLNVLGTCEYMD
YTGTPQVIYTDPQIGIAGEKDKAVKFLKFPYNADTRAIIKGLREGFALIGIDKNGTIVYSEIIGDNAEELINIMALAIRK
RVTIRDLAFTIFIHPSLSEILVNAARGEFDLDVDLYK
>Mature_437_residues
MEYDVIIIGGGTAGYTAGVILGRRGKKIAVVEKEKFGGTCVNYGCVPSIFLSDISFLYSRLSEIGNYKGIEINLKLNNFF
SKREEIIDYLSTAGKRLIENASGETIEGEGIIRDNHTVEVNGRLLTTKEIIIATGSKPKPPRIEGIENAISEDQVVRLNT
IPSSMVIIGGGVAGTEIAQIFAKLGTKVTLLTRSKILKELHEETRKLMIDSLEFDGIDIIENSKIEKIYGEKVYTNKGVF
DGEIVVYATGREPNYPKGFEKLNVEIGKEGIIVNERMQTNIKNIYAIGDIVNKEKKVAHVAYMEAIIASLNVLGTCEYMD
YTGTPQVIYTDPQIGIAGEKDKAVKFLKFPYNADTRAIIKGLREGFALIGIDKNGTIVYSEIIGDNAEELINIMALAIRK
RVTIRDLAFTIFIHPSLSEILVNAARGEFDLDVDLYK

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=469, Percent_Identity=30.0639658848614, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI50301238, Length=475, Percent_Identity=27.1578947368421, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI22035672, Length=473, Percent_Identity=25.369978858351, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI291045266, Length=479, Percent_Identity=25.8872651356994, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI148277071, Length=475, Percent_Identity=25.6842105263158, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI148277065, Length=475, Percent_Identity=25.6842105263158, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI33519430, Length=473, Percent_Identity=25.7928118393235, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI33519428, Length=473, Percent_Identity=25.7928118393235, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI33519426, Length=473, Percent_Identity=25.7928118393235, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI291045268, Length=464, Percent_Identity=25.6465517241379, Blast_Score=89, Evalue=8e-18,
Organism=Escherichia coli, GI1786307, Length=454, Percent_Identity=26.431718061674, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1789915, Length=425, Percent_Identity=27.0588235294118, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI87082354, Length=469, Percent_Identity=26.4392324093817, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87081717, Length=452, Percent_Identity=25, Blast_Score=112, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI32565766, Length=461, Percent_Identity=29.7180043383948, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17557007, Length=479, Percent_Identity=23.5908141962422, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI71983429, Length=436, Percent_Identity=28.2110091743119, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71983419, Length=436, Percent_Identity=28.2110091743119, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71982272, Length=474, Percent_Identity=25.7383966244726, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321091, Length=470, Percent_Identity=30.8510638297872, Blast_Score=163, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6325166, Length=459, Percent_Identity=28.1045751633987, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6325240, Length=469, Percent_Identity=26.6524520255864, Blast_Score=121, Evalue=3e-28,
Organism=Drosophila melanogaster, GI21358499, Length=468, Percent_Identity=30.5555555555556, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24640553, Length=466, Percent_Identity=27.2532188841202, Blast_Score=132, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24640549, Length=466, Percent_Identity=27.2532188841202, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24640551, Length=467, Percent_Identity=27.6231263383298, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17737741, Length=470, Percent_Identity=24.2553191489362, Blast_Score=115, Evalue=6e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48248; Mature: 48248

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYDVIIIGGGTAGYTAGVILGRRGKKIAVVEKEKFGGTCVNYGCVPSIFLSDISFLYSR
CCEEEEEECCCCCCCEEEEEEECCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHH
LSEIGNYKGIEINLKLNNFFSKREEIIDYLSTAGKRLIENASGETIEGEGIIRDNHTVEV
HHHHCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEECCCEEEE
NGRLLTTKEIIIATGSKPKPPRIEGIENAISEDQVVRLNTIPSSMVIIGGGVAGTEIAQI
CCEEEEEEEEEEEECCCCCCCCCHHHHHHCCCCCEEEEECCCCEEEEEECCCCHHHHHHH
FAKLGTKVTLLTRSKILKELHEETRKLMIDSLEFDGIDIIENSKIEKIYGEKVYTNKGVF
HHHHCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHCCEEECCCCCC
DGEIVVYATGREPNYPKGFEKLNVEIGKEGIIVNERMQTNIKNIYAIGDIVNKEKKVAHV
CCEEEEEECCCCCCCCCCCCEEEEEECCCCEEECHHHHCCHHHEEEEHHHHCCHHHHHHH
AYMEAIIASLNVLGTCEYMDYTGTPQVIYTDPQIGIAGEKDKAVKFLKFPYNADTRAIIK
HHHHHHHHHHHHEEEEEECCCCCCCCEEEECCCCCCCCCCCCCEEEEECCCCCCHHHHHH
GLREGFALIGIDKNGTIVYSEIIGDNAEELINIMALAIRKRVTIRDLAFTIFIHPSLSEI
HHHCCEEEEEECCCCCEEEHHHHCCCHHHHHHHHHHHHHCCCEEEEEEEEEEECCCHHHH
LVNAARGEFDLDVDLYK
HHHHCCCCEEEEEEECC
>Mature Secondary Structure
MEYDVIIIGGGTAGYTAGVILGRRGKKIAVVEKEKFGGTCVNYGCVPSIFLSDISFLYSR
CCEEEEEECCCCCCCEEEEEEECCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHH
LSEIGNYKGIEINLKLNNFFSKREEIIDYLSTAGKRLIENASGETIEGEGIIRDNHTVEV
HHHHCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEECCCEEEE
NGRLLTTKEIIIATGSKPKPPRIEGIENAISEDQVVRLNTIPSSMVIIGGGVAGTEIAQI
CCEEEEEEEEEEEECCCCCCCCCHHHHHHCCCCCEEEEECCCCEEEEEECCCCHHHHHHH
FAKLGTKVTLLTRSKILKELHEETRKLMIDSLEFDGIDIIENSKIEKIYGEKVYTNKGVF
HHHHCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHCCEEECCCCCC
DGEIVVYATGREPNYPKGFEKLNVEIGKEGIIVNERMQTNIKNIYAIGDIVNKEKKVAHV
CCEEEEEECCCCCCCCCCCCEEEEEECCCCEEECHHHHCCHHHEEEEHHHHCCHHHHHHH
AYMEAIIASLNVLGTCEYMDYTGTPQVIYTDPQIGIAGEKDKAVKFLKFPYNADTRAIIK
HHHHHHHHHHHHEEEEEECCCCCCCCEEEECCCCCCCCCCCCCEEEEECCCCCCHHHHHH
GLREGFALIGIDKNGTIVYSEIIGDNAEELINIMALAIRKRVTIRDLAFTIFIHPSLSEI
HHHCCEEEEEECCCCCEEEHHHHCCCHHHHHHHHHHHHHCCCEEEEEEEEEEECCCHHHH
LVNAARGEFDLDVDLYK
HHHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]