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The map label for this gene is thiC

Identifier: 15922177

GI number: 15922177

Start: 1875828

End: 1877117

Strand: Reverse

Name: thiC

Synonym: ST1864

Alternate gene names: 15922177

Gene position: 1877117-1875828 (Counterclockwise)

Preceding gene: 15922179

Following gene: 15922176

Centisome position: 69.66

GC content: 35.27

Gene sequence:

>1290_bases
ATGGCAATAATTGATGATGCACGAAAAGGTATTATAACTGATGAAATGAAAAAGATAAGTCTAATTGAGAAAATTTCACC
AGAAAAAGTTAGGAAAAGAATAGTAGAAGGAAAAATTATGCTAATTAGAAATGAAAAATATCCTTCTAAAAAGATAGTTG
CAATAGGTAAGGGTTTAACAACTAAAGTTAATATAAACATAGGAACATCTAGTGAAGTCGTCAATCTTGATATGGAATTA
GAAAAGGTGAAGATTGCAAATAAATGGGGAGATACATTAATGGACTTATCAACTGGAGGAGATTTAGATTTAATTAGAAG
GGAAATAATAAAACACTCTGAGCTACCAGTAGGTACTGTTCCAGTTTATCAAGTGTTTATAGAATCTTTTAAGCATAAAT
CTGGTGGAGCATATTTCACAGAAGATGATTTGTTAAATACTATAGAAAAACATTTAAAAGATGGGGTTGCTTTTATGACG
ATTCATGCGGGTTTAACAAAGGATTTAGCTATAAGGGCTTTGAAAAGCAACAGGATAATCCCTATAGTATCTAGAGGAGG
AGATATGATAGCAGGGTGGATGATTCATAATAACTCTGAGAATCCATATAGAAAGAACTGGGATTATATATTAGAAATGT
TTAAACAATATGATGCAGTAATCTCATTAGGAGACGCTTTAAGACCTGGAGCTACTGGAGATGCTCATGATGAGTTCCAG
ATAGGAGAATTATTAGAAACAGCGAGACTAGTAAAGAGTGCACTTGAGAAAGGAGTTCAGGTAATGGTAGAAGGACCAGG
TCATGTTCCATTAAATGAGATAGCGTGGGACGTAAAGTTAATGAAAAAGCTTACTGGTGGTGTTCCTTATTACGTTTTAG
GTCCTTTGCCAATAGATGTTGGTGCACCATATGATCATATTGCCTCAGCTATAGGTGCGGCAATTGCCTCAGCAAGTGGT
GCTGACTTATTATGTTATCTAACTCCAGCAGAACACTTAAGTTTACCAACTGTTGAACAAGTTGAAGAAGGAGCGATAGC
TTATAGAATTGCCGCTCATGCTGGAGATATTGTAAAATTGGGTAAAAAAGTTAGAAACTGGGATGATGTAGTAAGTTATT
ATAGAGGAAAATTGGAATGGGAAAAAATGATATCGTCATTAATAGATCCCGAAAGGGCTATGAAAGTCTATACGCAATTT
GGTAAACCGAAAGTTAGGGCATGTACTATGTGTGGTGGTTATTGTCCTATGATGTGGGCAATGGAGCAGGTAAGGAAAAT
TAGTGACTAG

Upstream 100 bases:

>100_bases
TATCTATTACTACTAATTAAGCTTTATCACAGAAAAATGTAAAGTTAGTACACAAACTGAATATAATAAGGTTTATAAGT
AACTTTTTTGATATATTTTT

Downstream 100 bases:

>100_bases
AATCGAAAATAATTTAATCACTTTCTCAACTTACTCTCTTAAATATGTCATTACCATGGAATGTTGTTAATGAGAAGAAT
ATTTATTTTAGTCATCAAGA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MAIIDDARKGIITDEMKKISLIEKISPEKVRKRIVEGKIMLIRNEKYPSKKIVAIGKGLTTKVNINIGTSSEVVNLDMEL
EKVKIANKWGDTLMDLSTGGDLDLIRREIIKHSELPVGTVPVYQVFIESFKHKSGGAYFTEDDLLNTIEKHLKDGVAFMT
IHAGLTKDLAIRALKSNRIIPIVSRGGDMIAGWMIHNNSENPYRKNWDYILEMFKQYDAVISLGDALRPGATGDAHDEFQ
IGELLETARLVKSALEKGVQVMVEGPGHVPLNEIAWDVKLMKKLTGGVPYYVLGPLPIDVGAPYDHIASAIGAAIASASG
ADLLCYLTPAEHLSLPTVEQVEEGAIAYRIAAHAGDIVKLGKKVRNWDDVVSYYRGKLEWEKMISSLIDPERAMKVYTQF
GKPKVRACTMCGGYCPMMWAMEQVRKISD

Sequences:

>Translated_429_residues
MAIIDDARKGIITDEMKKISLIEKISPEKVRKRIVEGKIMLIRNEKYPSKKIVAIGKGLTTKVNINIGTSSEVVNLDMEL
EKVKIANKWGDTLMDLSTGGDLDLIRREIIKHSELPVGTVPVYQVFIESFKHKSGGAYFTEDDLLNTIEKHLKDGVAFMT
IHAGLTKDLAIRALKSNRIIPIVSRGGDMIAGWMIHNNSENPYRKNWDYILEMFKQYDAVISLGDALRPGATGDAHDEFQ
IGELLETARLVKSALEKGVQVMVEGPGHVPLNEIAWDVKLMKKLTGGVPYYVLGPLPIDVGAPYDHIASAIGAAIASASG
ADLLCYLTPAEHLSLPTVEQVEEGAIAYRIAAHAGDIVKLGKKVRNWDDVVSYYRGKLEWEKMISSLIDPERAMKVYTQF
GKPKVRACTMCGGYCPMMWAMEQVRKISD
>Mature_428_residues
AIIDDARKGIITDEMKKISLIEKISPEKVRKRIVEGKIMLIRNEKYPSKKIVAIGKGLTTKVNINIGTSSEVVNLDMELE
KVKIANKWGDTLMDLSTGGDLDLIRREIIKHSELPVGTVPVYQVFIESFKHKSGGAYFTEDDLLNTIEKHLKDGVAFMTI
HAGLTKDLAIRALKSNRIIPIVSRGGDMIAGWMIHNNSENPYRKNWDYILEMFKQYDAVISLGDALRPGATGDAHDEFQI
GELLETARLVKSALEKGVQVMVEGPGHVPLNEIAWDVKLMKKLTGGVPYYVLGPLPIDVGAPYDHIASAIGAAIASASGA
DLLCYLTPAEHLSLPTVEQVEEGAIAYRIAAHAGDIVKLGKKVRNWDDVVSYYRGKLEWEKMISSLIDPERAMKVYTQFG
KPKVRACTMCGGYCPMMWAMEQVRKISD

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=451, Percent_Identity=36.1419068736142, Blast_Score=269, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_SULTO (Q96ZH0)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377846.1
- GeneID:   1459920
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1864
- NMPDR:   fig|273063.1.peg.2014
- HOGENOM:   HBG285234
- OMA:   VDFMTIH
- ProtClustDB:   PRK13352
- BioCyc:   STOK273063:ST1864-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 47568; Mature: 47437

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: NA

Important sites: BINDING 66-66 BINDING 94-94 BINDING 123-123 BINDING 162-162 BINDING 264-264 BINDING 291-291

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIIDDARKGIITDEMKKISLIEKISPEKVRKRIVEGKIMLIRNEKYPSKKIVAIGKGLT
CCCCCCHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCCCEEEEECCCCE
TKVNINIGTSSEVVNLDMELEKVKIANKWGDTLMDLSTGGDLDLIRREIIKHSELPVGTV
EEEEEEECCCCCEEEECCHHHHHHHHHHHCCHHEECCCCCCHHHHHHHHHHHCCCCCCCH
PVYQVFIESFKHKSGGAYFTEDDLLNTIEKHLKDGVAFMTIHAGLTKDLAIRALKSNRII
HHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCCEE
PIVSRGGDMIAGWMIHNNSENPYRKNWDYILEMFKQYDAVISLGDALRPGATGDAHDEFQ
EEEECCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
IGELLETARLVKSALEKGVQVMVEGPGHVPLNEIAWDVKLMKKLTGGVPYYVLGPLPIDV
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEECCEEECC
GAPYDHIASAIGAAIASASGADLLCYLTPAEHLSLPTVEQVEEGAIAYRIAAHAGDIVKL
CCCHHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCCCHHHHHCCCEEEEEHHCCCHHHHH
GKKVRNWDDVVSYYRGKLEWEKMISSLIDPERAMKVYTQFGKPKVRACTMCGGYCPMMWA
HHHHCCHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHH
MEQVRKISD
HHHHHHCCC
>Mature Secondary Structure 
AIIDDARKGIITDEMKKISLIEKISPEKVRKRIVEGKIMLIRNEKYPSKKIVAIGKGLT
CCCCCHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCCCEEEEECCCCE
TKVNINIGTSSEVVNLDMELEKVKIANKWGDTLMDLSTGGDLDLIRREIIKHSELPVGTV
EEEEEEECCCCCEEEECCHHHHHHHHHHHCCHHEECCCCCCHHHHHHHHHHHCCCCCCCH
PVYQVFIESFKHKSGGAYFTEDDLLNTIEKHLKDGVAFMTIHAGLTKDLAIRALKSNRII
HHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCCEE
PIVSRGGDMIAGWMIHNNSENPYRKNWDYILEMFKQYDAVISLGDALRPGATGDAHDEFQ
EEEECCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
IGELLETARLVKSALEKGVQVMVEGPGHVPLNEIAWDVKLMKKLTGGVPYYVLGPLPIDV
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEECCEEECC
GAPYDHIASAIGAAIASASGADLLCYLTPAEHLSLPTVEQVEEGAIAYRIAAHAGDIVKL
CCCHHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCCCHHHHHCCCEEEEEHHCCCHHHHH
GKKVRNWDDVVSYYRGKLEWEKMISSLIDPERAMKVYTQFGKPKVRACTMCGGYCPMMWA
HHHHCCHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHH
MEQVRKISD
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479