Click here to switch to the map view.

The map label for this gene is nikB [H]

Identifier: 15921952

GI number: 15921952

Start: 1640150

End: 1641379

Strand: Reverse

Name: nikB [H]

Synonym: ST1649

Alternate gene names: 15921952

Gene position: 1641379-1640150 (Counterclockwise)

Preceding gene: 15921953

Following gene: 15921951

Centisome position: 60.91

GC content: 30.65

Gene sequence:

>1230_bases
GTGAAAAGCTCATATTTACTTTATATTTCTATAATTCTCTTAATACTAGGTTTAATTATTTCGTTTATTTCGATAGAATC
TAAACAAATTATGCTCTTTTATTATAATGAGAGTAATATCGCTTATCCACAACCAGGATGGCAAGACTATTATTGGAGTG
TGTTAGTATATGGAAATCCGAACGCTACAATAATTACCTTCAATGAGACTATATTTCTAAAGAATGTTACCATATATCAT
TTTAATACAACGACAAGATACGTGTATATTGTACATGGTTATATACAACCATATGCATATCTTTCTCCATTTTCCCTTTT
TTTAGTATTAACAGCCTCATTTATTGGATTTAAAGGAACAACTTTATTCTTCCAATCAAGAATACTAGGAGAAGAGCTAA
CAAAAGGTTACGCTGTAGGAGGTTCCTTATATAGATACGTGCTCAAAAGATTTTCCAGCTTCCTAATCTCTATTATTCTA
GTAATAACTGTAGTTATTATACTCGAATCGCTTCATGGAAGAAATATAATAAAAACTATTTATGACATGAGTTTATTTAA
TTTAGGTAACAGTTCATATTTTGGAATTTCAGTTACTAGCCTAGTTTTAACAGCCTTAGCTTATACTTCTTTACTTCTAG
GAATCTCCTTTGCACTAACTGTATATTTAAGTGCATTTCTTTCAATTTATAGTTTGACCAATAGGAGGCTAAGAAATATT
ATTGATAAATGGAAATACATTGGTACCGCATTAGCATCATGGGTACTATCAATAATAGTAATTTACCTTTTACATTTCTT
CCTCGACATTTTCCCTTATGGTACTCCAAAAGAGAATATATTCCCGTATTTATTCATGCCACTCATATCCTTATTTTTCC
CCTATGTAGGAATATTTAGTAATAGACTTCTATCTAATGTAAAAAGTATTCCAGCTAATTACAAGGGATTAAAAGAAACC
ATCCTAATTTATAGGCATTTACTAGGTAATGTGACGGTAGTAATGTTATCATCAATATCCTCAGCATTTATTGAAATGTT
ACTTGCTGAAATGCTAGTTGAAGGAATATTCTTATGGCCAGGTTTAGGTGAGTTATTAAAAATAGCCATATTTCATGGGG
ACTATAAAATAGTAGAAGGAGTGATGGTACTCTATTCTACTATTGCTCTGTTATCTAATCTGATAACTGACATTATTTAT
GGTGTTATGGATCCAAGGGTGACTAGGTAA

Upstream 100 bases:

>100_bases
AAAACTTACGATCCTAATGATTTGCTTAAGTATTTAAGAGAGAAGTATCTAAGCATTTGATCTTAGATAAATATTTAAGC
ATACTGTTGAACCCTTTTTT

Downstream 100 bases:

>100_bases
TGATTAGACTTTACGTATTGTTAGGACTATTTATTTCTATTGTAACTTTAAGTATATTCCTTGACTTTTATCAATTACCT
AAAGGGAAACCATTAACACC

Product: peptide transporter permease

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MKSSYLLYISIILLILGLIISFISIESKQIMLFYYNESNIAYPQPGWQDYYWSVLVYGNPNATIITFNETIFLKNVTIYH
FNTTTRYVYIVHGYIQPYAYLSPFSLFLVLTASFIGFKGTTLFFQSRILGEELTKGYAVGGSLYRYVLKRFSSFLISIIL
VITVVIILESLHGRNIIKTIYDMSLFNLGNSSYFGISVTSLVLTALAYTSLLLGISFALTVYLSAFLSIYSLTNRRLRNI
IDKWKYIGTALASWVLSIIVIYLLHFFLDIFPYGTPKENIFPYLFMPLISLFFPYVGIFSNRLLSNVKSIPANYKGLKET
ILIYRHLLGNVTVVMLSSISSAFIEMLLAEMLVEGIFLWPGLGELLKIAIFHGDYKIVEGVMVLYSTIALLSNLITDIIY
GVMDPRVTR

Sequences:

>Translated_409_residues
MKSSYLLYISIILLILGLIISFISIESKQIMLFYYNESNIAYPQPGWQDYYWSVLVYGNPNATIITFNETIFLKNVTIYH
FNTTTRYVYIVHGYIQPYAYLSPFSLFLVLTASFIGFKGTTLFFQSRILGEELTKGYAVGGSLYRYVLKRFSSFLISIIL
VITVVIILESLHGRNIIKTIYDMSLFNLGNSSYFGISVTSLVLTALAYTSLLLGISFALTVYLSAFLSIYSLTNRRLRNI
IDKWKYIGTALASWVLSIIVIYLLHFFLDIFPYGTPKENIFPYLFMPLISLFFPYVGIFSNRLLSNVKSIPANYKGLKET
ILIYRHLLGNVTVVMLSSISSAFIEMLLAEMLVEGIFLWPGLGELLKIAIFHGDYKIVEGVMVLYSTIALLSNLITDIIY
GVMDPRVTR
>Mature_409_residues
MKSSYLLYISIILLILGLIISFISIESKQIMLFYYNESNIAYPQPGWQDYYWSVLVYGNPNATIITFNETIFLKNVTIYH
FNTTTRYVYIVHGYIQPYAYLSPFSLFLVLTASFIGFKGTTLFFQSRILGEELTKGYAVGGSLYRYVLKRFSSFLISIIL
VITVVIILESLHGRNIIKTIYDMSLFNLGNSSYFGISVTSLVLTALAYTSLLLGISFALTVYLSAFLSIYSLTNRRLRNI
IDKWKYIGTALASWVLSIIVIYLLHFFLDIFPYGTPKENIFPYLFMPLISLFFPYVGIFSNRLLSNVKSIPANYKGLKET
ILIYRHLLGNVTVVMLSSISSAFIEMLLAEMLVEGIFLWPGLGELLKIAIFHGDYKIVEGVMVLYSTIALLSNLITDIIY
GVMDPRVTR

Specific function: Involved in a nickel transport system, probably translocates nickel through the bacterial inner membrane [H]

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=234, Percent_Identity=24.7863247863248, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787497, Length=227, Percent_Identity=24.6696035242291, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1789965, Length=96, Percent_Identity=37.5, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1788504, Length=258, Percent_Identity=27.1317829457364, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR014156 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 46598; Mature: 46598

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSYLLYISIILLILGLIISFISIESKQIMLFYYNESNIAYPQPGWQDYYWSVLVYGNP
CCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEEECC
NATIITFNETIFLKNVTIYHFNTTTRYVYIVHGYIQPYAYLSPFSLFLVLTASFIGFKGT
CEEEEEEECEEEEEEEEEEEEECCEEEEEEEEEHHCCHHHHHHHHHHHHHHHHHHCCCCH
TLFFQSRILGEELTKGYAVGGSLYRYVLKRFSSFLISIILVITVVIILESLHGRNIIKTI
HHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
YDMSLFNLGNSSYFGISVTSLVLTALAYTSLLLGISFALTVYLSAFLSIYSLTNRRLRNI
HHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDKWKYIGTALASWVLSIIVIYLLHFFLDIFPYGTPKENIFPYLFMPLISLFFPYVGIFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
NRLLSNVKSIPANYKGLKETILIYRHLLGNVTVVMLSSISSAFIEMLLAEMLVEGIFLWP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GLGELLKIAIFHGDYKIVEGVMVLYSTIALLSNLITDIIYGVMDPRVTR
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKSSYLLYISIILLILGLIISFISIESKQIMLFYYNESNIAYPQPGWQDYYWSVLVYGNP
CCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEEECC
NATIITFNETIFLKNVTIYHFNTTTRYVYIVHGYIQPYAYLSPFSLFLVLTASFIGFKGT
CEEEEEEECEEEEEEEEEEEEECCEEEEEEEEEHHCCHHHHHHHHHHHHHHHHHHCCCCH
TLFFQSRILGEELTKGYAVGGSLYRYVLKRFSSFLISIILVITVVIILESLHGRNIIKTI
HHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
YDMSLFNLGNSSYFGISVTSLVLTALAYTSLLLGISFALTVYLSAFLSIYSLTNRRLRNI
HHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDKWKYIGTALASWVLSIIVIYLLHFFLDIFPYGTPKENIFPYLFMPLISLFFPYVGIFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
NRLLSNVKSIPANYKGLKETILIYRHLLGNVTVVMLSSISSAFIEMLLAEMLVEGIFLWP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GLGELLKIAIFHGDYKIVEGVMVLYSTIALLSNLITDIIYGVMDPRVTR
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]