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The map label for this gene is ndhK [H]

Identifier: 15921820

GI number: 15921820

Start: 1520281

End: 1520802

Strand: Direct

Name: ndhK [H]

Synonym: ST1527

Alternate gene names: 15921820

Gene position: 1520281-1520802 (Clockwise)

Preceding gene: 15921817

Following gene: 15921822

Centisome position: 56.42

GC content: 36.97

Gene sequence:

>522_bases
ATGACTGAAGAACTACTTTTAACAGGAAACTTACAAGAAGCATCTAAGAAAGCAATCCAATGGTTGATAAACAGAAAACC
GATAAAGAACTTAATAGATTGGGGTATTTCATTTTCACTATGGCCACCACATTTAACAACCAGCTGTTGTGGAACAGAAT
TTGGAGCTTTTGCTGCAGCTAGATTTGATGCTGAGAGATTTGGTGTTCTTCCATTTTCCTCTGCAAGGCAAACTAATTTA
TTAGTTATTGAAGGCACTTTAACTAGAAAAATGGCAAGAGCAGCGAAAATTGTCTATGATCAAATGCCAGAACCCAAATG
GGTAATAGCTATGGGAGCTTGCTCACTTGAAGGTGGAATATTTTGGAACTCCTATAATACTGTCTTACCCTCAGAAATTG
GGATTCCAGTAGACATTTATGCCCCAGGCTGTCCAACTAGACCAGAAGCATTAGCAAGAGCTATAATAACACTTCAAAAA
AGAATAAGAAGTAATCAAACAGTAAAAGCTAAATCTGCATAA

Upstream 100 bases:

>100_bases
TAGAAATGTATATACATTTTTTTCCAATGAAACACAAATTGTACGACTTTTATTAACAAAAGGATTTTAAGAATGCCTCT
AATTGTAATATGGGATTATA

Downstream 100 bases:

>100_bases
TCTACGTCTAATATTTTTCCTTTTACTGGAGTCTTATACTCTGGTTTAACTATAGCAGGTAAATCTGAAATTTCAATACC
GTTCCAAAGCTTAAGATATT

Product: NADH-ubiquinone oxidoreductase subunit K

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I subunit K; NDH-1 subunit K; NDH-K [H]

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MTEELLLTGNLQEASKKAIQWLINRKPIKNLIDWGISFSLWPPHLTTSCCGTEFGAFAAARFDAERFGVLPFSSARQTNL
LVIEGTLTRKMARAAKIVYDQMPEPKWVIAMGACSLEGGIFWNSYNTVLPSEIGIPVDIYAPGCPTRPEALARAIITLQK
RIRSNQTVKAKSA

Sequences:

>Translated_173_residues
MTEELLLTGNLQEASKKAIQWLINRKPIKNLIDWGISFSLWPPHLTTSCCGTEFGAFAAARFDAERFGVLPFSSARQTNL
LVIEGTLTRKMARAAKIVYDQMPEPKWVIAMGACSLEGGIFWNSYNTVLPSEIGIPVDIYAPGCPTRPEALARAIITLQK
RIRSNQTVKAKSA
>Mature_172_residues
TEELLLTGNLQEASKKAIQWLINRKPIKNLIDWGISFSLWPPHLTTSCCGTEFGAFAAARFDAERFGVLPFSSARQTNLL
VIEGTLTRKMARAAKIVYDQMPEPKWVIAMGACSLEGGIFWNSYNTVLPSEIGIPVDIYAPGCPTRPEALARAIITLQKR
IRSNQTVKAKSA

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=142, Percent_Identity=42.9577464788732, Blast_Score=137, Evalue=4e-33,
Organism=Escherichia coli, GI1788624, Length=152, Percent_Identity=41.4473684210526, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=37.7049180327869, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1789074, Length=123, Percent_Identity=37.3983739837398, Blast_Score=80, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17509685, Length=155, Percent_Identity=41.9354838709677, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI18859983, Length=150, Percent_Identity=43.3333333333333, Blast_Score=144, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24642371, Length=150, Percent_Identity=43.3333333333333, Blast_Score=144, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24651058, Length=142, Percent_Identity=45.0704225352113, Blast_Score=142, Evalue=1e-34,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 19125; Mature: 18994

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEELLLTGNLQEASKKAIQWLINRKPIKNLIDWGISFSLWPPHLTTSCCGTEFGAFAAA
CCCCEEEECCHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCCCCHHHHCCHHHHHHHH
RFDAERFGVLPFSSARQTNLLVIEGTLTRKMARAAKIVYDQMPEPKWVIAMGACSLEGGI
HCCHHHHCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCEEEEEECEECCCCE
FWNSYNTVLPSEIGIPVDIYAPGCPTRPEALARAIITLQKRIRSNQTVKAKSA
EECCCCCCCCHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCC
>Mature Secondary Structure 
TEELLLTGNLQEASKKAIQWLINRKPIKNLIDWGISFSLWPPHLTTSCCGTEFGAFAAA
CCCEEEECCHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCCCCHHHHCCHHHHHHHH
RFDAERFGVLPFSSARQTNLLVIEGTLTRKMARAAKIVYDQMPEPKWVIAMGACSLEGGI
HCCHHHHCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCEEEEEECEECCCCE
FWNSYNTVLPSEIGIPVDIYAPGCPTRPEALARAIITLQKRIRSNQTVKAKSA
EECCCCCCCCHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA