Click here to switch to the map view.

The map label for this gene is purF [H]

Identifier: 15921791

GI number: 15921791

Start: 1492846

End: 1494057

Strand: Direct

Name: purF [H]

Synonym: ST1497

Alternate gene names: 15921791

Gene position: 1492846-1494057 (Clockwise)

Preceding gene: 15921790

Following gene: 15921792

Centisome position: 55.4

GC content: 32.67

Gene sequence:

>1212_bases
ATGGCTGGAATAATAGGTGTATATGCATTTGATAAAATTTGGAATATAAGTAAATTTTTATATTATGGTCTAATAGGATT
ACAGAATAGAGGATATAGTTACTCAGGAATGTCGATACTTAAAGAAAACTTCCAATTAATAACTAACGAAGGTGCACCAG
AAGATATTGAGCTACCACCGAATATAGAAGGATGGGCAGGGATAGCTTATACTGGAACTAAAATAGGATATCCAATAATA
ACAGATTTTGGCACTTTAGTTGTAGATGGAATTATTAAGGGAGATTTAAATGAAATCGCAAAAGGATTATATAAAGATCC
AGAGAATACTTTAAAAGAAATCAATGGTGTTTTCTCATTAATATTCTTAGCAAAAGATGGAAAAATGTTGGGTTATAGAG
ATTCATATGGAATAAAACCTCTAGAAATAGGAGGTTTTGGTTTTGATTTAGCTATTCTATCATCAGAGACTTCGGGAATT
ACGGTTATAGGTGGAGAATTCAGAAGAGAAATTAAACCTGGTGAAGCGGTATTTATTGACACTTATGAAATTTCTTACAG
CCAAATAAATGAGAGTAGACATAATTACTGCGCGATTGATTTAGTTTATCAATCAAGAATAGACAGTTTTGTATTTAGTA
AAAATATTTATGAAGTAAGAGTGAAGATTGGAGAACAGCTAGCTGAAGAAAAGAAAATTGATGCTGATGTTGTGATTGGC
GTTCCAGATACTGCTATACCTTTTGCAATAGGCTATTCTAAGAAGTCTGGTATACCTTATGATTTAGGTTTTACCAGAAC
TGGAAGTCCGATTAGAACAATGCTGGCTTCAGATGATTTCTTAAAGATCATAGGAGTTCAACTAAAATTAAATCCTATTA
AATACGTAGTCAAAGGCAAAAGAGTAATCTTAATTGACGATTCTATGGTCACTGGCAGAACTTTGAAAAATACAGTTTTT
GCTTTGAGAAGTTTAGGTGCTAAGGAGGTTCACGTTTTAATTGGCAGTCCTAAGTTAATATCAAAATGTCCTTATGGTAT
GGAAGTTCCAGAGGAAAAAGATTTAATTGCTGCTAATCTCTCAGAAGAAGAAATAGCTAAGGTTATTGGTGCTGATTCCA
TTTACTGGTTAAGTCTTGAAGGTCTTTATAAAGTTCTAGGTAAGAGCATATGTGTTGGTTGTATGACTAGAAACTATCCT
AAGGTGATATAA

Upstream 100 bases:

>100_bases
AAGACCTGATTTATGTCATGCATGTTTTTCTGGAGTTTATCCCCTTAAATTTTCATATAATTTGCAAGTATTAGAATCTA
TATTTAAAAAGGTGAGTTAA

Downstream 100 bases:

>100_bases
GATGAAAGTTTTGTTAATCGGAGATGGAGCAAGAGAAAACGCTCTAGCAGAAGCATTAGCTAACTCTCCTAAAGGTTATA
AGATTCATGCAATTTCTTCA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MAGIIGVYAFDKIWNISKFLYYGLIGLQNRGYSYSGMSILKENFQLITNEGAPEDIELPPNIEGWAGIAYTGTKIGYPII
TDFGTLVVDGIIKGDLNEIAKGLYKDPENTLKEINGVFSLIFLAKDGKMLGYRDSYGIKPLEIGGFGFDLAILSSETSGI
TVIGGEFRREIKPGEAVFIDTYEISYSQINESRHNYCAIDLVYQSRIDSFVFSKNIYEVRVKIGEQLAEEKKIDADVVIG
VPDTAIPFAIGYSKKSGIPYDLGFTRTGSPIRTMLASDDFLKIIGVQLKLNPIKYVVKGKRVILIDDSMVTGRTLKNTVF
ALRSLGAKEVHVLIGSPKLISKCPYGMEVPEEKDLIAANLSEEEIAKVIGADSIYWLSLEGLYKVLGKSICVGCMTRNYP
KVI

Sequences:

>Translated_403_residues
MAGIIGVYAFDKIWNISKFLYYGLIGLQNRGYSYSGMSILKENFQLITNEGAPEDIELPPNIEGWAGIAYTGTKIGYPII
TDFGTLVVDGIIKGDLNEIAKGLYKDPENTLKEINGVFSLIFLAKDGKMLGYRDSYGIKPLEIGGFGFDLAILSSETSGI
TVIGGEFRREIKPGEAVFIDTYEISYSQINESRHNYCAIDLVYQSRIDSFVFSKNIYEVRVKIGEQLAEEKKIDADVVIG
VPDTAIPFAIGYSKKSGIPYDLGFTRTGSPIRTMLASDDFLKIIGVQLKLNPIKYVVKGKRVILIDDSMVTGRTLKNTVF
ALRSLGAKEVHVLIGSPKLISKCPYGMEVPEEKDLIAANLSEEEIAKVIGADSIYWLSLEGLYKVLGKSICVGCMTRNYP
KVI
>Mature_402_residues
AGIIGVYAFDKIWNISKFLYYGLIGLQNRGYSYSGMSILKENFQLITNEGAPEDIELPPNIEGWAGIAYTGTKIGYPIIT
DFGTLVVDGIIKGDLNEIAKGLYKDPENTLKEINGVFSLIFLAKDGKMLGYRDSYGIKPLEIGGFGFDLAILSSETSGIT
VIGGEFRREIKPGEAVFIDTYEISYSQINESRHNYCAIDLVYQSRIDSFVFSKNIYEVRVKIGEQLAEEKKIDADVVIGV
PDTAIPFAIGYSKKSGIPYDLGFTRTGSPIRTMLASDDFLKIIGVQLKLNPIKYVVKGKRVILIDDSMVTGRTLKNTVFA
LRSLGAKEVHVLIGSPKLISKCPYGMEVPEEKDLIAANLSEEEIAKVIGADSIYWLSLEGLYKVLGKSICVGCMTRNYPK
VI

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=301, Percent_Identity=32.890365448505, Blast_Score=160, Evalue=1e-39,
Organism=Escherichia coli, GI1788651, Length=284, Percent_Identity=30.6338028169014, Blast_Score=135, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17554892, Length=481, Percent_Identity=26.8191268191268, Blast_Score=164, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6323958, Length=452, Percent_Identity=28.0973451327434, Blast_Score=133, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24659604, Length=310, Percent_Identity=34.1935483870968, Blast_Score=175, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24659598, Length=310, Percent_Identity=34.1935483870968, Blast_Score=175, Evalue=5e-44,
Organism=Drosophila melanogaster, GI28573187, Length=313, Percent_Identity=34.5047923322684, Blast_Score=174, Evalue=8e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 44466; Mature: 44335

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGIIGVYAFDKIWNISKFLYYGLIGLQNRGYSYSGMSILKENFQLITNEGAPEDIELPP
CCCEEEEHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHCCEEEECCCCCCCCCCCC
NIEGWAGIAYTGTKIGYPIITDFGTLVVDGIIKGDLNEIAKGLYKDPENTLKEINGVFSL
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHEEE
IFLAKDGKMLGYRDSYGIKPLEIGGFGFDLAILSSETSGITVIGGEFRREIKPGEAVFID
EEEECCCCEEEECCCCCCCEEEECCCCEEEEEEECCCCCEEEECHHHHHCCCCCCEEEEE
TYEISYSQINESRHNYCAIDLVYQSRIDSFVFSKNIYEVRVKIGEQLAEEKKIDADVVIG
EEECCHHHHHCCCCCEEEEEEEEHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCCEEEEE
VPDTAIPFAIGYSKKSGIPYDLGFTRTGSPIRTMLASDDFLKIIGVQLKLNPIKYVVKGK
CCCCCCCEEEECCCCCCCCEECCCCCCCCHHHHHHCCCCEEEEEEEEEEECCEEEEECCC
RVILIDDSMVTGRTLKNTVFALRSLGAKEVHVLIGSPKLISKCPYGMEVPEEKDLIAANL
EEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHCCCCCCCCCCCCEEEECC
SEEEIAKVIGADSIYWLSLEGLYKVLGKSICVGCMTRNYPKVI
CHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AGIIGVYAFDKIWNISKFLYYGLIGLQNRGYSYSGMSILKENFQLITNEGAPEDIELPP
CCEEEEHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHCCEEEECCCCCCCCCCCC
NIEGWAGIAYTGTKIGYPIITDFGTLVVDGIIKGDLNEIAKGLYKDPENTLKEINGVFSL
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHEEE
IFLAKDGKMLGYRDSYGIKPLEIGGFGFDLAILSSETSGITVIGGEFRREIKPGEAVFID
EEEECCCCEEEECCCCCCCEEEECCCCEEEEEEECCCCCEEEECHHHHHCCCCCCEEEEE
TYEISYSQINESRHNYCAIDLVYQSRIDSFVFSKNIYEVRVKIGEQLAEEKKIDADVVIG
EEECCHHHHHCCCCCEEEEEEEEHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCCEEEEE
VPDTAIPFAIGYSKKSGIPYDLGFTRTGSPIRTMLASDDFLKIIGVQLKLNPIKYVVKGK
CCCCCCCEEEECCCCCCCCEECCCCCCCCHHHHHHCCCCEEEEEEEEEEECCEEEEECCC
RVILIDDSMVTGRTLKNTVFALRSLGAKEVHVLIGSPKLISKCPYGMEVPEEKDLIAANL
EEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHCCCCCCCCCCCCEEEECC
SEEEIAKVIGADSIYWLSLEGLYKVLGKSICVGCMTRNYPKVI
CHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]