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The map label for this gene is pheS

Identifier: 15921705

GI number: 15921705

Start: 1419598

End: 1421007

Strand: Reverse

Name: pheS

Synonym: ST1416

Alternate gene names: 15921705

Gene position: 1421007-1419598 (Counterclockwise)

Preceding gene: 15921707

Following gene: 15921704

Centisome position: 52.73

GC content: 31.99

Gene sequence:

>1410_bases
ATGTTAAGTGAAAACGAAATTAAAATTCTAGAGTATTTAAGTAAGAAAAGGGAAGCTTCAGCTGAGGATATAAGCAAACA
ACTTAACATACCCATAGAAAGCGTTTTCAGTATTTCACAGTTACTAAAAGAAAAAGGATACATAGAAGTTGAGGAAAAAA
AAGTTATAAAATATGAGCTTACTGATGAAGGAAAAAGAAGATTAAAGGAGGGTTTTCCAGAGGAAAAACTAGTTAAATTA
TTAGATGGAAAAGAGAAAAAAATACATGAATTAAAGGATAAACTAGGAAGAGATTTTGAAATTTCTATTGGATGGGCTAA
AAGAAAAGGGCTTGTAAAAATAGATGGAGACACAGTCATACCACTTGTTAAAGACTATGAGGCAAAGGAAGAAAAAGAAG
CATTAATAAATCCAGAAAAAGCAAACAAAGATATTTTGCAGCAATTATTAAATAGAAAACTACTCATAAAAAAAGAGGAA
AAAATATTGAATATAAGACTTATAAGAGAACCCTTAGAAATCAAACCGGCATTAATATTTTTAACTCCAGAGCTTTTAGC
TAGCGGGGAATGGAAAAAATATGCTTTAAGGGAATATAACGTAGAAGCTTATCCTCCTTTTTACCCTATAGGGAAAAAAC
ATTTCTTTAAAGAATTCCTAGAGAGATTAAGAGATTTAATGAGAGACTTAGGATTTAAAGAAGTATACAGTGATTATGTA
GAGATAGAATTTTATAACTTTGATCTTCTATTTCAAGCTCAAGACCACCCTGCTAGAGAAATTCATGATAGTTTTGCAAT
AAGTGGTAAAGGTAAATTAACCGACGAGAAATTAATACAGAGAGTTAAACAAATCCATGAGAGAGGATGGAAGTATAATT
GGGATGTCAATATTTCAATGAGACTTATGTTAAGAAGTCAAACTACAGCAACTACTGCAAGAGTTTTAGCATCAAGACCT
AAACCACCAATAAGAGTTTTCACGATAGGTAAAGTCTTCAGACCAGATTCGATAGATGCTACACACTTAATTGAATTTCA
TCAACTAGATGGTTTAGTAATAGAAGATAATTTCACTTTTAGAGATTTATTAGGTACTCTTAAGGAAATATTCAACGGTA
TCGGAATTAAAGAAATAAGATTTAAACCAGCCTATTTTCCATTCACTGAACCAAGCGTGGAAGTTTACGGAAAAATAGAG
GGATTAGGATGGGTTGAGATGGCTGGGGCAGGTTTGTTAAGACCAGAAATCCTAGAAGCAGTTGAAATTAGCTCATCTGC
TGGTGCATGGGGATTAGGAATAGATAGATTAGCTATGTTACTTCTTGGTCTTAAAGATATAAGATTATTATACGCTACAG
ATATTGAATACCTAAGAAATAGAAAGGTTGAGATAAATGCCGACAATTAA

Upstream 100 bases:

>100_bases
GGATCTAATAAAAGTAGATCCTAATGATTTTGTAAAAGAAGTTTCAAAAAGAGGATATAAAGCTTTAGTACTTCAGCCAG
GGCAGAGTGTGGAGTTATAG

Downstream 100 bases:

>100_bases
TATATACAAGTGGCGTCTATTAAATGAGTTAAAAATTAGTGAAACCCAATTAGAGGATTTATTATTTAATCTAAAGTCTG
AAATGAAACCAATAGACCAA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MLSENEIKILEYLSKKREASAEDISKQLNIPIESVFSISQLLKEKGYIEVEEKKVIKYELTDEGKRRLKEGFPEEKLVKL
LDGKEKKIHELKDKLGRDFEISIGWAKRKGLVKIDGDTVIPLVKDYEAKEEKEALINPEKANKDILQQLLNRKLLIKKEE
KILNIRLIREPLEIKPALIFLTPELLASGEWKKYALREYNVEAYPPFYPIGKKHFFKEFLERLRDLMRDLGFKEVYSDYV
EIEFYNFDLLFQAQDHPAREIHDSFAISGKGKLTDEKLIQRVKQIHERGWKYNWDVNISMRLMLRSQTTATTARVLASRP
KPPIRVFTIGKVFRPDSIDATHLIEFHQLDGLVIEDNFTFRDLLGTLKEIFNGIGIKEIRFKPAYFPFTEPSVEVYGKIE
GLGWVEMAGAGLLRPEILEAVEISSSAGAWGLGIDRLAMLLLGLKDIRLLYATDIEYLRNRKVEINADN

Sequences:

>Translated_469_residues
MLSENEIKILEYLSKKREASAEDISKQLNIPIESVFSISQLLKEKGYIEVEEKKVIKYELTDEGKRRLKEGFPEEKLVKL
LDGKEKKIHELKDKLGRDFEISIGWAKRKGLVKIDGDTVIPLVKDYEAKEEKEALINPEKANKDILQQLLNRKLLIKKEE
KILNIRLIREPLEIKPALIFLTPELLASGEWKKYALREYNVEAYPPFYPIGKKHFFKEFLERLRDLMRDLGFKEVYSDYV
EIEFYNFDLLFQAQDHPAREIHDSFAISGKGKLTDEKLIQRVKQIHERGWKYNWDVNISMRLMLRSQTTATTARVLASRP
KPPIRVFTIGKVFRPDSIDATHLIEFHQLDGLVIEDNFTFRDLLGTLKEIFNGIGIKEIRFKPAYFPFTEPSVEVYGKIE
GLGWVEMAGAGLLRPEILEAVEISSSAGAWGLGIDRLAMLLLGLKDIRLLYATDIEYLRNRKVEINADN
>Mature_469_residues
MLSENEIKILEYLSKKREASAEDISKQLNIPIESVFSISQLLKEKGYIEVEEKKVIKYELTDEGKRRLKEGFPEEKLVKL
LDGKEKKIHELKDKLGRDFEISIGWAKRKGLVKIDGDTVIPLVKDYEAKEEKEALINPEKANKDILQQLLNRKLLIKKEE
KILNIRLIREPLEIKPALIFLTPELLASGEWKKYALREYNVEAYPPFYPIGKKHFFKEFLERLRDLMRDLGFKEVYSDYV
EIEFYNFDLLFQAQDHPAREIHDSFAISGKGKLTDEKLIQRVKQIHERGWKYNWDVNISMRLMLRSQTTATTARVLASRP
KPPIRVFTIGKVFRPDSIDATHLIEFHQLDGLVIEDNFTFRDLLGTLKEIFNGIGIKEIRFKPAYFPFTEPSVEVYGKIE
GLGWVEMAGAGLLRPEILEAVEISSSAGAWGLGIDRLAMLLLGLKDIRLLYATDIEYLRNRKVEINADN

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=482, Percent_Identity=30.9128630705394, Blast_Score=222, Evalue=5e-58,
Organism=Escherichia coli, GI1788007, Length=257, Percent_Identity=31.9066147859922, Blast_Score=139, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI32563657, Length=477, Percent_Identity=30.188679245283, Blast_Score=225, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI17508957, Length=477, Percent_Identity=30.188679245283, Blast_Score=225, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI32566635, Length=263, Percent_Identity=25.0950570342205, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321087, Length=456, Percent_Identity=31.7982456140351, Blast_Score=213, Evalue=4e-56,
Organism=Drosophila melanogaster, GI18858079, Length=479, Percent_Identity=29.4363256784969, Blast_Score=194, Evalue=1e-49,
Organism=Drosophila melanogaster, GI17137424, Length=216, Percent_Identity=29.6296296296296, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_SULTO (Q971D7)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377374.1
- ProteinModelPortal:   Q971D7
- SMR:   Q971D7
- GeneID:   1459445
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1416
- NMPDR:   fig|273063.1.peg.1542
- HOGENOM:   HBG497668
- OMA:   PEFYQCE
- ProtClustDB:   PRK04172
- BioCyc:   STOK273063:ST1416-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00282
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR022917
- InterPro:   IPR002319
- InterPro:   IPR002831
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF01978 TrmB; PF01409 tRNA-synt_2d

EC number: =6.1.1.20

Molecular weight: Translated: 54398; Mature: 54398

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSENEIKILEYLSKKREASAEDISKQLNIPIESVFSISQLLKEKGYIEVEEKKVIKYEL
CCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEECCEEEEEEE
TDEGKRRLKEGFPEEKLVKLLDGKEKKIHELKDKLGRDFEISIGWAKRKGLVKIDGDTVI
CCHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEECCCCEEEECCCEEE
PLVKDYEAKEEKEALINPEKANKDILQQLLNRKLLIKKEEKILNIRLIREPLEIKPALIF
CHHHCCCCHHHHHHHCCCCCCCHHHHHHHHCCCEEEECHHHHEEEEEECCCCCCCCEEEE
LTPELLASGEWKKYALREYNVEAYPPFYPIGKKHFFKEFLERLRDLMRDLGFKEVYSDYV
EEHHHHCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCEE
EIEFYNFDLLFQAQDHPAREIHDSFAISGKGKLTDEKLIQRVKQIHERGWKYNWDVNISM
EEEEEEEEEEEEECCCCHHHHHHHHEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEE
RLMLRSQTTATTARVLASRPKPPIRVFTIGKVFRPDSIDATHLIEFHQLDGLVIEDNFTF
EEEEECCCHHHHHHHHHCCCCCCEEEEEECCEECCCCCCHHHHHEECCCCCEEEECCCCH
RDLLGTLKEIFNGIGIKEIRFKPAYFPFTEPSVEVYGKIEGLGWVEMAGAGLLRPEILEA
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCHHHCCCCCCCHHHHHH
VEISSSAGAWGLGIDRLAMLLLGLKDIRLLYATDIEYLRNRKVEINADN
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCCEEEECCC
>Mature Secondary Structure
MLSENEIKILEYLSKKREASAEDISKQLNIPIESVFSISQLLKEKGYIEVEEKKVIKYEL
CCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEECCEEEEEEE
TDEGKRRLKEGFPEEKLVKLLDGKEKKIHELKDKLGRDFEISIGWAKRKGLVKIDGDTVI
CCHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEECCCCEEEECCCEEE
PLVKDYEAKEEKEALINPEKANKDILQQLLNRKLLIKKEEKILNIRLIREPLEIKPALIF
CHHHCCCCHHHHHHHCCCCCCCHHHHHHHHCCCEEEECHHHHEEEEEECCCCCCCCEEEE
LTPELLASGEWKKYALREYNVEAYPPFYPIGKKHFFKEFLERLRDLMRDLGFKEVYSDYV
EEHHHHCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCEE
EIEFYNFDLLFQAQDHPAREIHDSFAISGKGKLTDEKLIQRVKQIHERGWKYNWDVNISM
EEEEEEEEEEEEECCCCHHHHHHHHEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEE
RLMLRSQTTATTARVLASRPKPPIRVFTIGKVFRPDSIDATHLIEFHQLDGLVIEDNFTF
EEEEECCCHHHHHHHHHCCCCCCEEEEEECCEECCCCCCHHHHHEECCCCCEEEECCCCH
RDLLGTLKEIFNGIGIKEIRFKPAYFPFTEPSVEVYGKIEGLGWVEMAGAGLLRPEILEA
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCHHHCCCCCCCHHHHHH
VEISSSAGAWGLGIDRLAMLLLGLKDIRLLYATDIEYLRNRKVEINADN
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479