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The map label for this gene is nth [C]

Identifier: 15921690

GI number: 15921690

Start: 1406436

End: 1407110

Strand: Direct

Name: nth [C]

Synonym: ST1401

Alternate gene names: 15921690

Gene position: 1406436-1407110 (Clockwise)

Preceding gene: 15921689

Following gene: 15921691

Centisome position: 52.19

GC content: 30.07

Gene sequence:

>675_bases
GTGTGTAACCCAAAAGAACTTTTAAATAAATTATCTAATGTTTATTCTATAGACCCAAAGGATTTTGTAGCATACTATGT
GTGTAAAAACACAAGTGATGTGTTTAAAACATTTGTTGCTACAATTCTCTCACAGAATTCTACTGATAAAGCAACATATG
TTGCATATAATAATCTTGAAAATAAAATTGGAGTTACGGTTGATAAGATTTTAAGCATAAGTGAAGATGAACTTAAGGAA
GTTATTAAGATTGTAGGTCTTTCCAATTCAAAAGCAAGGTATATAAAGAATATAGCATTATTTTTCAAAAGAAATAAAAT
AGATGAGCTTACAAGACTTCCTTGCGACAAATTAAGGGAATTATTTCTTACTGTAGACGGAATTGGTGAAAAAACCGCTG
ATGTTGTTCTGGTAAATTGTTTTAAATGTAAGTTTTTCCCAGTGGATACACATATAAAAAGAGTTATGAGCCGTCTAGGT
ATTTTAGGGTCAAAACCACAATATAAAGAAATTGCTGATTTCTTTATATCGTCACTTAATGAAGATGAATTGCTAGAATT
ACATCAGTTGCTAATACTTCACGGAAGAAAAACTTGCACTGCAAAAAAACCTTTATGCGATAAATGTGTAATAAATTATT
GCTGTGAATATTTCTCTCGAATGGGTAAGCACTAA

Upstream 100 bases:

>100_bases
AACGTGTCCTTTCTTAATTGCTTCTAACACTAATTTTATCTCATACATTAATTATCACATTTATTTCAAACCTTTTTAAG
GTAAATAGCAAATATATCTA

Downstream 100 bases:

>100_bases
GGATTTATTACCTCATGAAGATATCATACCCTCTATAGTAGAAGAAAATGTCTTAAATATAAAGAAAAAACAAAAAATTG
TTCCTATAATAGTAGATACC

Product: endonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MCNPKELLNKLSNVYSIDPKDFVAYYVCKNTSDVFKTFVATILSQNSTDKATYVAYNNLENKIGVTVDKILSISEDELKE
VIKIVGLSNSKARYIKNIALFFKRNKIDELTRLPCDKLRELFLTVDGIGEKTADVVLVNCFKCKFFPVDTHIKRVMSRLG
ILGSKPQYKEIADFFISSLNEDELLELHQLLILHGRKTCTAKKPLCDKCVINYCCEYFSRMGKH

Sequences:

>Translated_224_residues
MCNPKELLNKLSNVYSIDPKDFVAYYVCKNTSDVFKTFVATILSQNSTDKATYVAYNNLENKIGVTVDKILSISEDELKE
VIKIVGLSNSKARYIKNIALFFKRNKIDELTRLPCDKLRELFLTVDGIGEKTADVVLVNCFKCKFFPVDTHIKRVMSRLG
ILGSKPQYKEIADFFISSLNEDELLELHQLLILHGRKTCTAKKPLCDKCVINYCCEYFSRMGKH
>Mature_224_residues
MCNPKELLNKLSNVYSIDPKDFVAYYVCKNTSDVFKTFVATILSQNSTDKATYVAYNNLENKIGVTVDKILSISEDELKE
VIKIVGLSNSKARYIKNIALFFKRNKIDELTRLPCDKLRELFLTVDGIGEKTADVVLVNCFKCKFFPVDTHIKRVMSRLG
ILGSKPQYKEIADFFISSLNEDELLELHQLLILHGRKTCTAKKPLCDKCVINYCCEYFSRMGKH

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Escherichia coli, GI1787920, Length=193, Percent_Identity=33.160621761658, Blast_Score=89, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17554540, Length=190, Percent_Identity=27.3684210526316, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00730 HhH-GPD [H]

EC number: 4.2.99.18

Molecular weight: Translated: 25629; Mature: 25629

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCNPKELLNKLSNVYSIDPKDFVAYYVCKNTSDVFKTFVATILSQNSTDKATYVAYNNLE
CCCHHHHHHHHHHHCCCCHHHHEEHHEECCCHHHHHHHHHHHHCCCCCCCEEEEEECCCC
NKIGVTVDKILSISEDELKEVIKIVGLSNSKARYIKNIALFFKRNKIDELTRLPCDKLRE
CHHCCHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHCCHHHHHH
LFLTVDGIGEKTADVVLVNCFKCKFFPVDTHIKRVMSRLGILGSKPQYKEIADFFISSLN
HHHHHCCCCCHHHHHHEEEEHHEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC
EDELLELHQLLILHGRKTCTAKKPLCDKCVINYCCEYFSRMGKH
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCNPKELLNKLSNVYSIDPKDFVAYYVCKNTSDVFKTFVATILSQNSTDKATYVAYNNLE
CCCHHHHHHHHHHHCCCCHHHHEEHHEECCCHHHHHHHHHHHHCCCCCCCEEEEEECCCC
NKIGVTVDKILSISEDELKEVIKIVGLSNSKARYIKNIALFFKRNKIDELTRLPCDKLRE
CHHCCHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHCCHHHHHH
LFLTVDGIGEKTADVVLVNCFKCKFFPVDTHIKRVMSRLGILGSKPQYKEIADFFISSLN
HHHHHCCCCCHHHHHHEEEEHHEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC
EDELLELHQLLILHGRKTCTAKKPLCDKCVINYCCEYFSRMGKH
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Carbon-Oxygen Lyase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]