Click here to switch to the map view.

The map label for this gene is pgk

Identifier: 15921641

GI number: 15921641

Start: 1363837

End: 1365084

Strand: Reverse

Name: pgk

Synonym: ST1357

Alternate gene names: 15921641

Gene position: 1365084-1363837 (Counterclockwise)

Preceding gene: 15921642

Following gene: 15921640

Centisome position: 50.66

GC content: 34.46

Gene sequence:

>1248_bases
ATGATAAAAGTAGCTGATCTTAATATTCCTGTTATAGAAGATTTCGACATAAATGGGAGAAAAGTTTTACTAAGAATAGA
TATAAATTCACCCGTAGACCGAAAAACTGGAAAATTATTAGATGATTCCAGAATTAAAGCCCATGCTGAAACAATTAGAG
AATTACTCGATAAGCAAAATTCTGTAGTAATAATTTCCCACCAAGGTAGACCAGGAGATGATGATTTTATTTCTTTAGAA
GAACACTCTAAAATCCTATCTAAATATGTTGGTAGGGAAATTGAGTTCGTCGATGATGTTATAGGTCCTTATGCTAGAGA
AAAAATATCTAAACTAGGTAAAGGGGAAGCCATCTTATTAGAGAATGTTAGAATCGTATCTGAAGAATTAATAGAGGCTC
CTCCACAACAGCAATCTAAAACTTTTTTAGTAAGAAAATTAGCCCCATTAGTCGATTTTTACATTAACGATGCTTTCGCA
ACAGCACATAGAAGCCAACCAAGTCTAGTAGGATTTCCTTTAGTAAAACCTTCAGCTGCAGGAAGAATAATGGAGAAAGA
AGTTTCTGCATTATCTAAGATCTTTAACAAGGAAGACTCTCCAAAAATATTTGTTTTGGGAGGAAGTAAGGTTAGTGATA
CATTAAAGATAATTGAACACCTAGCAAAAAATAGAGTTGCTGATAGAATATTAACTGGCGGTTTAGTAGCAGAACTATTT
GCTGTTGCGAAAGGTATAAATTTAGGTAAGCCTAATATGCAAGTTTTAGAGAATAAGGGTCTTCTGAGCCTAATTCCGAG
AGCTAGAAAAATCTTGCTATCTGGTGCACCTATAGAAATCCCTGTTGACTTTACAGTGGAAAAACCTAGTGGAGAATTAA
GCAATGATCCGGAGAATAATGTGAGTGGTATTATAAAAGATATAGGGAATACTACCATTGAAATTTATTCCTCTTTTATT
AAGGAAGGCAAAGTCATTACATTAAGAGGACCTATGGGAGTTATTGAAGATGAAAGATTCAGAATTGGTACTAAAAGCTT
ACTGAAAGCCTCTATTGAAAGCCCGGGATATGTAATTATTGGTGGCGGTCATATGATAAGTATGCTTGATAAAGACGTAG
AAATAAATCCAAATAAAATTCATGTATCTACCGGAGGAGGAGCGTTGCTTTTATTTTTAGCCGGTGATAAGTTACCGGCA
TTAGAAGCTTTACATATGTCATGGGTGATGCAAAGTGGAAAAAGTTAA

Upstream 100 bases:

>100_bases
ATTTAATACCACTATTATGGGGTAACATATTAGCATCTAATATTGTTTGCTAAAATACTACTTATTTATATACCTTTTTT
CTAATCTTAAACATGAGATT

Downstream 100 bases:

>100_bases
GGTAGCTGTAAATGGTTACGGGACTATTGGAAAAAGAGTAGCTGATGCGATAAGATTACAACCAGACATGGAATTAATAG
GAGTTGGTAAAACATCACCA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MIKVADLNIPVIEDFDINGRKVLLRIDINSPVDRKTGKLLDDSRIKAHAETIRELLDKQNSVVIISHQGRPGDDDFISLE
EHSKILSKYVGREIEFVDDVIGPYAREKISKLGKGEAILLENVRIVSEELIEAPPQQQSKTFLVRKLAPLVDFYINDAFA
TAHRSQPSLVGFPLVKPSAAGRIMEKEVSALSKIFNKEDSPKIFVLGGSKVSDTLKIIEHLAKNRVADRILTGGLVAELF
AVAKGINLGKPNMQVLENKGLLSLIPRARKILLSGAPIEIPVDFTVEKPSGELSNDPENNVSGIIKDIGNTTIEIYSSFI
KEGKVITLRGPMGVIEDERFRIGTKSLLKASIESPGYVIIGGGHMISMLDKDVEINPNKIHVSTGGGALLLFLAGDKLPA
LEALHMSWVMQSGKS

Sequences:

>Translated_415_residues
MIKVADLNIPVIEDFDINGRKVLLRIDINSPVDRKTGKLLDDSRIKAHAETIRELLDKQNSVVIISHQGRPGDDDFISLE
EHSKILSKYVGREIEFVDDVIGPYAREKISKLGKGEAILLENVRIVSEELIEAPPQQQSKTFLVRKLAPLVDFYINDAFA
TAHRSQPSLVGFPLVKPSAAGRIMEKEVSALSKIFNKEDSPKIFVLGGSKVSDTLKIIEHLAKNRVADRILTGGLVAELF
AVAKGINLGKPNMQVLENKGLLSLIPRARKILLSGAPIEIPVDFTVEKPSGELSNDPENNVSGIIKDIGNTTIEIYSSFI
KEGKVITLRGPMGVIEDERFRIGTKSLLKASIESPGYVIIGGGHMISMLDKDVEINPNKIHVSTGGGALLLFLAGDKLPA
LEALHMSWVMQSGKS
>Mature_415_residues
MIKVADLNIPVIEDFDINGRKVLLRIDINSPVDRKTGKLLDDSRIKAHAETIRELLDKQNSVVIISHQGRPGDDDFISLE
EHSKILSKYVGREIEFVDDVIGPYAREKISKLGKGEAILLENVRIVSEELIEAPPQQQSKTFLVRKLAPLVDFYINDAFA
TAHRSQPSLVGFPLVKPSAAGRIMEKEVSALSKIFNKEDSPKIFVLGGSKVSDTLKIIEHLAKNRVADRILTGGLVAELF
AVAKGINLGKPNMQVLENKGLLSLIPRARKILLSGAPIEIPVDFTVEKPSGELSNDPENNVSGIIKDIGNTTIEIYSSFI
KEGKVITLRGPMGVIEDERFRIGTKSLLKASIESPGYVIIGGGHMISMLDKDVEINPNKIHVSTGGGALLLFLAGDKLPA
LEALHMSWVMQSGKS

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=417, Percent_Identity=31.6546762589928, Blast_Score=175, Evalue=8e-44,
Organism=Homo sapiens, GI31543397, Length=417, Percent_Identity=30.2158273381295, Blast_Score=162, Evalue=4e-40,
Organism=Escherichia coli, GI1789294, Length=403, Percent_Identity=31.2655086848635, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17508823, Length=420, Percent_Identity=31.4285714285714, Blast_Score=177, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6319857, Length=413, Percent_Identity=31.7191283292978, Blast_Score=171, Evalue=2e-43,
Organism=Drosophila melanogaster, GI17136394, Length=417, Percent_Identity=33.3333333333333, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24581203, Length=423, Percent_Identity=31.2056737588652, Blast_Score=170, Evalue=2e-42,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_SULTO (Q971K1)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377310.1
- ProteinModelPortal:   Q971K1
- SMR:   Q971K1
- GeneID:   1459379
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1357
- NMPDR:   fig|273063.1.peg.1478
- HOGENOM:   HBG453500
- OMA:   PGKKDFT
- ProtClustDB:   PRK00073
- BioCyc:   STOK273063:ST1357-MONOMER
- BRENDA:   2.7.2.3
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 45384; Mature: 45384

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 44-44 BINDING 124-124 BINDING 164-164 BINDING 336-336

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVADLNIPVIEDFDINGRKVLLRIDINSPVDRKTGKLLDDSRIKAHAETIRELLDKQN
CEEEEECCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
SVVIISHQGRPGDDDFISLEEHSKILSKYVGREIEFVDDVIGPYAREKISKLGKGEAILL
CEEEEEECCCCCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHCCCCCEEEE
ENVRIVSEELIEAPPQQQSKTFLVRKLAPLVDFYINDAFATAHRSQPSLVGFPLVKPSAA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHH
GRIMEKEVSALSKIFNKEDSPKIFVLGGSKVSDTLKIIEHLAKNRVADRILTGGLVAELF
HHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVAKGINLGKPNMQVLENKGLLSLIPRARKILLSGAPIEIPVDFTVEKPSGELSNDPENN
HHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHEECCCCEEEEEEEEEECCCCCCCCCCCCH
VSGIIKDIGNTTIEIYSSFIKEGKVITLRGPMGVIEDERFRIGTKSLLKASIESPGYVII
HHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCEECCHHHHHHHHCCCCCEEEE
GGGHMISMLDKDVEINPNKIHVSTGGGALLLFLAGDKLPALEALHMSWVMQSGKS
ECCEEEEECCCCCEECCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIKVADLNIPVIEDFDINGRKVLLRIDINSPVDRKTGKLLDDSRIKAHAETIRELLDKQN
CEEEEECCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
SVVIISHQGRPGDDDFISLEEHSKILSKYVGREIEFVDDVIGPYAREKISKLGKGEAILL
CEEEEEECCCCCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHCCCCCEEEE
ENVRIVSEELIEAPPQQQSKTFLVRKLAPLVDFYINDAFATAHRSQPSLVGFPLVKPSAA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHH
GRIMEKEVSALSKIFNKEDSPKIFVLGGSKVSDTLKIIEHLAKNRVADRILTGGLVAELF
HHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVAKGINLGKPNMQVLENKGLLSLIPRARKILLSGAPIEIPVDFTVEKPSGELSNDPENN
HHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHEECCCCEEEEEEEEEECCCCCCCCCCCCH
VSGIIKDIGNTTIEIYSSFIKEGKVITLRGPMGVIEDERFRIGTKSLLKASIESPGYVII
HHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCEECCHHHHHHHHCCCCCEEEE
GGGHMISMLDKDVEINPNKIHVSTGGGALLLFLAGDKLPALEALHMSWVMQSGKS
ECCEEEEECCCCCEECCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479