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The map label for this gene is tfbB [H]

Identifier: 15921535

GI number: 15921535

Start: 1270177

End: 1271064

Strand: Reverse

Name: tfbB [H]

Synonym: ST1271

Alternate gene names: 15921535

Gene position: 1271064-1270177 (Counterclockwise)

Preceding gene: 15921536

Following gene: 15921534

Centisome position: 47.17

GC content: 30.86

Gene sequence:

>888_bases
ATGGAGAAAATAAAGTGCCCTAATTGCGGAAGTGAAAGTATAACGTTAGATCTAAACAAAGGCACATACATCTGTACTAA
TTGTGGATATGTCATTGATGAATTTGTTATAGATCAAGGTCCAGAATGGAGAGCGTATACAGAACAAGACAGATTGGAAA
GAGAAAGAACAGGTTCTCCTATTACTGCAAAAGTTCATGATTTTGGAATAACAACAACAATTGGATATTCTAGGTCCAGT
AATAAAACTAAAATAGAAAAATTAAGAGCAATACAGAATAAACTCAGAGTTTCTCCAAAGGATAGAAAGTTAGTCACTTA
TTTATCCGTATTAAATAATGAAGCTGCTAAGCTAAATCTTCCTGAATACGTAAAAGAAACAGCCTCTCTTTTAATAAGAA
AAATAATCGATGAGGGAAAAGCTAAAAGAATAGACATATATACGTTAATTGCTGCGGTACTTTACTATTCTTGTCAAGTA
AATAAAATACCAAAATCCCTTCAAGAGATAAAGAATAATTATGGTATTTCATCTAGTGAATTATGGAAAGCACTAGAGAG
AGTTCAAAAAGTAGCTAAGAGTTCATTAGAGTTTAAACCCAATATAAAACCCACAGAGTTCATTCCAAAAATTGTAGAAA
AACTTAATTTACCACCATATATATCTACTAAAGCTTCTGAATTAGTTGACTTAATGCACAAAAATGGTCTAACTAGTGGA
AAAGGATACACAGCATTAGCAGCTGCTAGTGTATACCTTGTAAGTACACTAATGGATGCAAAAAAGACTCAAAAAGACAT
AGCTAATGCACTAAATATAACAGAAGTTACAATAAGAAATAGATATAAGGAAATTATATCAAATTTCGAAATAGAAGTTA
AACTTTAA

Upstream 100 bases:

>100_bases
GAAATGTGGAAGAACCATACATACTAGCATTACCGTTATCAGAAAAAGCACCCCAAGGTAAGTTATACGTAGAACTTAAA
GAAAAGAGGGGTAAGAAAAA

Downstream 100 bases:

>100_bases
GGACAACAAAATAAATAAGCTAGTTTTACAATATATTAATATAGCCAACACGGTGGTTTTATGGAATCAACTACATCATC
ATATGAAGATCTTGCTTATC

Product: transcription initiation factor IIB

Products: NA

Alternate protein names: TFIIB 2 [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MEKIKCPNCGSESITLDLNKGTYICTNCGYVIDEFVIDQGPEWRAYTEQDRLERERTGSPITAKVHDFGITTTIGYSRSS
NKTKIEKLRAIQNKLRVSPKDRKLVTYLSVLNNEAAKLNLPEYVKETASLLIRKIIDEGKAKRIDIYTLIAAVLYYSCQV
NKIPKSLQEIKNNYGISSSELWKALERVQKVAKSSLEFKPNIKPTEFIPKIVEKLNLPPYISTKASELVDLMHKNGLTSG
KGYTALAAASVYLVSTLMDAKKTQKDIANALNITEVTIRNRYKEIISNFEIEVKL

Sequences:

>Translated_295_residues
MEKIKCPNCGSESITLDLNKGTYICTNCGYVIDEFVIDQGPEWRAYTEQDRLERERTGSPITAKVHDFGITTTIGYSRSS
NKTKIEKLRAIQNKLRVSPKDRKLVTYLSVLNNEAAKLNLPEYVKETASLLIRKIIDEGKAKRIDIYTLIAAVLYYSCQV
NKIPKSLQEIKNNYGISSSELWKALERVQKVAKSSLEFKPNIKPTEFIPKIVEKLNLPPYISTKASELVDLMHKNGLTSG
KGYTALAAASVYLVSTLMDAKKTQKDIANALNITEVTIRNRYKEIISNFEIEVKL
>Mature_295_residues
MEKIKCPNCGSESITLDLNKGTYICTNCGYVIDEFVIDQGPEWRAYTEQDRLERERTGSPITAKVHDFGITTTIGYSRSS
NKTKIEKLRAIQNKLRVSPKDRKLVTYLSVLNNEAAKLNLPEYVKETASLLIRKIIDEGKAKRIDIYTLIAAVLYYSCQV
NKIPKSLQEIKNNYGISSSELWKALERVQKVAKSSLEFKPNIKPTEFIPKIVEKLNLPPYISTKASELVDLMHKNGLTSG
KGYTALAAASVYLVSTLMDAKKTQKDIANALNITEVTIRNRYKEIISNFEIEVKL

Specific function: Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre- initiation complex (DNA-TBP-TFIIB) [H]

COG id: COG1405

COG function: function code K; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TFIIB-type zinc finger [H]

Homologues:

Organism=Homo sapiens, GI4504193, Length=287, Percent_Identity=24.7386759581882, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI22035556, Length=283, Percent_Identity=21.9081272084806, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17564892, Length=285, Percent_Identity=23.1578947368421, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6325343, Length=293, Percent_Identity=22.5255972696246, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17136758, Length=268, Percent_Identity=26.1194029850746, Blast_Score=118, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006670
- InterPro:   IPR011028
- InterPro:   IPR013763
- InterPro:   IPR000812
- InterPro:   IPR013150
- InterPro:   IPR013137 [H]

Pfam domain/function: PF00382 TFIIB; PF08271 TFIIB_Zn_Ribbon [H]

EC number: NA

Molecular weight: Translated: 33319; Mature: 33319

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS51134 ZF_TFIIB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIKCPNCGSESITLDLNKGTYICTNCGYVIDEFVIDQGPEWRAYTEQDRLERERTGSP
CCCCCCCCCCCCCEEEEECCCEEEEECCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCC
ITAKVHDFGITTTIGYSRSSNKTKIEKLRAIQNKLRVSPKDRKLVTYLSVLNNEAAKLNL
CEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCC
PEYVKETASLLIRKIIDEGKAKRIDIYTLIAAVLYYSCQVNKIPKSLQEIKNNYGISSSE
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHH
LWKALERVQKVAKSSLEFKPNIKPTEFIPKIVEKLNLPPYISTKASELVDLMHKNGLTSG
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCC
KGYTALAAASVYLVSTLMDAKKTQKDIANALNITEVTIRNRYKEIISNFEIEVKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MEKIKCPNCGSESITLDLNKGTYICTNCGYVIDEFVIDQGPEWRAYTEQDRLERERTGSP
CCCCCCCCCCCCCEEEEECCCEEEEECCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCC
ITAKVHDFGITTTIGYSRSSNKTKIEKLRAIQNKLRVSPKDRKLVTYLSVLNNEAAKLNL
CEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCC
PEYVKETASLLIRKIIDEGKAKRIDIYTLIAAVLYYSCQVNKIPKSLQEIKNNYGISSSE
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHH
LWKALERVQKVAKSSLEFKPNIKPTEFIPKIVEKLNLPPYISTKASELVDLMHKNGLTSG
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCC
KGYTALAAASVYLVSTLMDAKKTQKDIANALNITEVTIRNRYKEIISNFEIEVKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]