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The map label for this gene is ppsA [H]

Identifier: 15921499

GI number: 15921499

Start: 1236825

End: 1239119

Strand: Reverse

Name: ppsA [H]

Synonym: ST1235

Alternate gene names: 15921499

Gene position: 1239119-1236825 (Counterclockwise)

Preceding gene: 15921500

Following gene: 15921497

Centisome position: 45.98

GC content: 34.16

Gene sequence:

>2295_bases
ATGATTAATATTGCTGGAGGGAAAGGAGCTAATTTAGGAGAATTAATATCAATGGGAATAAGAGTACCTCCGGGTTTTGT
CATAACTTCTCATGCGTATAAATATTTCATTTCATATAATAAATTGGATGAGAAAATAAAGGAAATTTTTGAAAAAGAAA
AAGATGATGAAATTATTAGTAGTAAAATTAGGGAATTAATTCTTTCAAGTCAAATTCCTCCAGATTTAGCTAATCAAATA
TTATCTGCATATGAAAACTTATCGAAACTTGTGGGCAAAGAAATTTTAGTAGCTGTAAGATCCTCAGCAACTGCAGAAGA
TATCGAGTCAGCAAGTTTCGCAGGTCAGCAAGAAACTTATCTAAATGTTAGTAAAGGAGAATTATTAGATGCAGTTAAAA
AAGTGTGGGCTAGTTTGTATACTGCAAGAGCAATAAGTTATAGAAGATTTAAAGGAATAGATCAGATAACTGTTGAAATG
GCAGTAGTAGTTCAAAAGATGGTTAATTCGAGATCTGCAGGGGTTATGTTTACACTTCATCCAGTCACAGGGGATAGGAA
CTATATTATGATAGAATCTAGTTGGGGATTAGGAGAAGCTGTAGTCAGCGGTAAAGTTACACCAGATGAAGTAGTTATTG
AAAAATCCTCTTTAAAAATAGTTGAGAAAAAAGTTTCACACAAAATTTTGAAAATAGTTTATGATAGAGAGAAAAGACAA
AATATAATAATTAACTTAAGCAATGAGGAAGCTGATCAAATGTCTATATCAGATGAAGAAGCAATTGAATTAGCTAGATT
AGCATTAAAGATAGAAGAACATTATGGAAGGCCTATGGATATAGAGTGGGCTATAGATGCAGATATGAAATTCCCAGATA
ATGTATTCATAGTTCAAGCTAGACCAGAAACTTATTGGACTAGTAAACAAATAGAGAGAAAGAAAGAAGAGGTTAAAGTT
AGTGTAGGAAAAGTACTTACTAAAGGTTTGCCTGCAAGCCCTGGTATAGCTTATGGAAAAGCTAGAGTTATCCTTGATGT
AAAAGAGGCTAAAGATTTTGAGAAAGGTGATATATTAGTAACTAAAATGACGGATCCAGATTGGGTTCCAATAATGAAAA
TTGCATCTGCGATAGTTACTGATGAAGGAGGAATGACTAGTCATGCCGCAATAGTTTCTAGAGAATTAGGTATCCCAGCT
GTAGTAGGTACAGGAAATGCTACGAAAGTTATTAAAGACGGTGAAGAAATCACAGTGGATGCTATTAGAGGAATAATATA
TGAGGGTAAATTGACATTTGAAACTGAGGAGAAAAAAGAGGCTACACAACCAGTAAGTGTTGGAGGAATAAGCAGAGATG
TCCTATTAAGTCTTTATCCTGTTACAGCAACTAAAATATACATGAACCTAGGACAGCCTGATATTATTCATAAATATCTT
GATTTACCATTTGATGGAATAGGGTTAATGAGGATTGAGTTTATAGTAAGTGAGTGGATCAAATATCATCCCTTATACTT
AATTAAGACTGGTAAACCTGAATTGTTTGTAGATAAATTAGCTGAAGGAATATCAATGGTTGCGTCTGCAATTTATCCTA
GACCAGTTGTTGTTAGGTTCTCTGATTTTAAGACAAACGAGTATAAGAGGTTAATTGGGGGTGAGGAGTTTGAACCAGAA
GAAAGAAATCCTATGATAGGATGGAGAGGAGTATCTAGATACGTTAGTCCACAATATGAACCAGCTTTTAGATTAGAGGT
TAGGGCAATACGTAAAGTTAGAGAAGAAATGGGATTAAAGAATGTATGGGTCATGTTCCCGTTTGTTAGAACCACCTGGG
AATTAGAAAAAGCAATAAAAATAATGGAAGAAGAGGGAGTAAGAAGAGGACAAGATTTTAAAGTATGGATCATGGCTGAA
GTTCCTTCAGTAGTAGTTTTAGCTGACAAATTCTCTCAACTCGTTGATGGCTTCAGTATAGGTAGTAATGACCTAGCCCA
GTTAACTTTAGGTGTAGATAGAGATTCCGATCTTCTTGCAAGAATGGGATATTATGATGAAAGAGATCCTGCAGTTCTAG
AATCAATAAAAAGATTAATAAAGGTTGCACATAAATATGGTAGGACAGTTTCTATATGTGGTCAAGCACCTAGTGTGTAT
CCAGAGATAAATGAATTTCTTGTTAGGCAAGGTATAGATAGTATAAGTGTAAACCCAGATGCCGTAATTCAGGTAAAAAG
ACAAGTGGCTTCAATAGAACAAAAGATAATGTTAGAAAAACTTAGGAAGAAATAA

Upstream 100 bases:

>100_bases
TTTTAATGTATAAGCCGGTTATTTTTATTGGGAGACAAATTTGGATTCTTCCCTTATCGAAGGGAGTCTCGTTACTGATC
TTAAAAATATAAGAAAAGAT

Downstream 100 bases:

>100_bases
AACATTCTTTACAATATCTTCTAGCCCCATCAAAATATGCAGTACATTCTTCACAAATTATCCTTCCGCATTTTTCGCAT
TTTCCTATAGATAAATTCTT

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 764; Mature: 764

Protein sequence:

>764_residues
MINIAGGKGANLGELISMGIRVPPGFVITSHAYKYFISYNKLDEKIKEIFEKEKDDEIISSKIRELILSSQIPPDLANQI
LSAYENLSKLVGKEILVAVRSSATAEDIESASFAGQQETYLNVSKGELLDAVKKVWASLYTARAISYRRFKGIDQITVEM
AVVVQKMVNSRSAGVMFTLHPVTGDRNYIMIESSWGLGEAVVSGKVTPDEVVIEKSSLKIVEKKVSHKILKIVYDREKRQ
NIIINLSNEEADQMSISDEEAIELARLALKIEEHYGRPMDIEWAIDADMKFPDNVFIVQARPETYWTSKQIERKKEEVKV
SVGKVLTKGLPASPGIAYGKARVILDVKEAKDFEKGDILVTKMTDPDWVPIMKIASAIVTDEGGMTSHAAIVSRELGIPA
VVGTGNATKVIKDGEEITVDAIRGIIYEGKLTFETEEKKEATQPVSVGGISRDVLLSLYPVTATKIYMNLGQPDIIHKYL
DLPFDGIGLMRIEFIVSEWIKYHPLYLIKTGKPELFVDKLAEGISMVASAIYPRPVVVRFSDFKTNEYKRLIGGEEFEPE
ERNPMIGWRGVSRYVSPQYEPAFRLEVRAIRKVREEMGLKNVWVMFPFVRTTWELEKAIKIMEEEGVRRGQDFKVWIMAE
VPSVVVLADKFSQLVDGFSIGSNDLAQLTLGVDRDSDLLARMGYYDERDPAVLESIKRLIKVAHKYGRTVSICGQAPSVY
PEINEFLVRQGIDSISVNPDAVIQVKRQVASIEQKIMLEKLRKK

Sequences:

>Translated_764_residues
MINIAGGKGANLGELISMGIRVPPGFVITSHAYKYFISYNKLDEKIKEIFEKEKDDEIISSKIRELILSSQIPPDLANQI
LSAYENLSKLVGKEILVAVRSSATAEDIESASFAGQQETYLNVSKGELLDAVKKVWASLYTARAISYRRFKGIDQITVEM
AVVVQKMVNSRSAGVMFTLHPVTGDRNYIMIESSWGLGEAVVSGKVTPDEVVIEKSSLKIVEKKVSHKILKIVYDREKRQ
NIIINLSNEEADQMSISDEEAIELARLALKIEEHYGRPMDIEWAIDADMKFPDNVFIVQARPETYWTSKQIERKKEEVKV
SVGKVLTKGLPASPGIAYGKARVILDVKEAKDFEKGDILVTKMTDPDWVPIMKIASAIVTDEGGMTSHAAIVSRELGIPA
VVGTGNATKVIKDGEEITVDAIRGIIYEGKLTFETEEKKEATQPVSVGGISRDVLLSLYPVTATKIYMNLGQPDIIHKYL
DLPFDGIGLMRIEFIVSEWIKYHPLYLIKTGKPELFVDKLAEGISMVASAIYPRPVVVRFSDFKTNEYKRLIGGEEFEPE
ERNPMIGWRGVSRYVSPQYEPAFRLEVRAIRKVREEMGLKNVWVMFPFVRTTWELEKAIKIMEEEGVRRGQDFKVWIMAE
VPSVVVLADKFSQLVDGFSIGSNDLAQLTLGVDRDSDLLARMGYYDERDPAVLESIKRLIKVAHKYGRTVSICGQAPSVY
PEINEFLVRQGIDSISVNPDAVIQVKRQVASIEQKIMLEKLRKK
>Mature_764_residues
MINIAGGKGANLGELISMGIRVPPGFVITSHAYKYFISYNKLDEKIKEIFEKEKDDEIISSKIRELILSSQIPPDLANQI
LSAYENLSKLVGKEILVAVRSSATAEDIESASFAGQQETYLNVSKGELLDAVKKVWASLYTARAISYRRFKGIDQITVEM
AVVVQKMVNSRSAGVMFTLHPVTGDRNYIMIESSWGLGEAVVSGKVTPDEVVIEKSSLKIVEKKVSHKILKIVYDREKRQ
NIIINLSNEEADQMSISDEEAIELARLALKIEEHYGRPMDIEWAIDADMKFPDNVFIVQARPETYWTSKQIERKKEEVKV
SVGKVLTKGLPASPGIAYGKARVILDVKEAKDFEKGDILVTKMTDPDWVPIMKIASAIVTDEGGMTSHAAIVSRELGIPA
VVGTGNATKVIKDGEEITVDAIRGIIYEGKLTFETEEKKEATQPVSVGGISRDVLLSLYPVTATKIYMNLGQPDIIHKYL
DLPFDGIGLMRIEFIVSEWIKYHPLYLIKTGKPELFVDKLAEGISMVASAIYPRPVVVRFSDFKTNEYKRLIGGEEFEPE
ERNPMIGWRGVSRYVSPQYEPAFRLEVRAIRKVREEMGLKNVWVMFPFVRTTWELEKAIKIMEEEGVRRGQDFKVWIMAE
VPSVVVLADKFSQLVDGFSIGSNDLAQLTLGVDRDSDLLARMGYYDERDPAVLESIKRLIKVAHKYGRTVSICGQAPSVY
PEINEFLVRQGIDSISVNPDAVIQVKRQVASIEQKIMLEKLRKK

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=796, Percent_Identity=41.7085427135678, Blast_Score=592, Evalue=1e-170,
Organism=Escherichia coli, GI1788756, Length=390, Percent_Identity=27.9487179487179, Blast_Score=132, Evalue=1e-31,
Organism=Escherichia coli, GI1788726, Length=388, Percent_Identity=26.8041237113402, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI1789193, Length=386, Percent_Identity=26.4248704663212, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI48994992, Length=356, Percent_Identity=26.9662921348315, Blast_Score=101, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17564524, Length=341, Percent_Identity=30.791788856305, Blast_Score=129, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 85797; Mature: 85797

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINIAGGKGANLGELISMGIRVPPGFVITSHAYKYFISYNKLDEKIKEIFEKEKDDEIIS
CEECCCCCCCCHHHHHHCCCCCCCCEEEECCCEEEEHHHHHHHHHHHHHHHCCCCHHHHH
SKIRELILSSQIPPDLANQILSAYENLSKLVGKEILVAVRSSATAEDIESASFAGQQETY
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCE
LNVSKGELLDAVKKVWASLYTARAISYRRFKGIDQITVEMAVVVQKMVNSRSAGVMFTLH
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEE
PVTGDRNYIMIESSWGLGEAVVSGKVTPDEVVIEKSSLKIVEKKVSHKILKIVYDREKRQ
ECCCCCCEEEEECCCCCCHHHHCCCCCCHHEEEEHHHHHHHHHHHHHHHHHHHHCCCCCC
NIIINLSNEEADQMSISDEEAIELARLALKIEEHYGRPMDIEWAIDADMKFPDNVFIVQA
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEE
RPETYWTSKQIERKKEEVKVSVGKVLTKGLPASPGIAYGKARVILDVKEAKDFEKGDILV
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEHHCCCCCCCCEEE
TKMTDPDWVPIMKIASAIVTDEGGMTSHAAIVSRELGIPAVVGTGNATKVIKDGEEITVD
EECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEECCCCCEEEECCCEEEHH
AIRGIIYEGKLTFETEEKKEATQPVSVGGISRDVLLSLYPVTATKIYMNLGQPDIIHKYL
HHHCEEEECEEEEECHHHHHHCCCCCCCCCCHHHHHEEECCEEEEEEEECCCHHHHHHHH
DLPFDGIGLMRIEFIVSEWIKYHPLYLIKTGKPELFVDKLAEGISMVASAIYPRPVVVRF
CCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
SDFKTNEYKRLIGGEEFEPEERNPMIGWRGVSRYVSPQYEPAFRLEVRAIRKVREEMGLK
ECCCCHHHHHHHCCCCCCCCCCCCCEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
NVWVMFPFVRTTWELEKAIKIMEEEGVRRGQDFKVWIMAEVPSVVVLADKFSQLVDGFSI
EEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEHHHHHHHHCCCCC
GSNDLAQLTLGVDRDSDLLARMGYYDERDPAVLESIKRLIKVAHKYGRTVSICGQAPSVY
CCCCCEEEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
PEINEFLVRQGIDSISVNPDAVIQVKRQVASIEQKIMLEKLRKK
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MINIAGGKGANLGELISMGIRVPPGFVITSHAYKYFISYNKLDEKIKEIFEKEKDDEIIS
CEECCCCCCCCHHHHHHCCCCCCCCEEEECCCEEEEHHHHHHHHHHHHHHHCCCCHHHHH
SKIRELILSSQIPPDLANQILSAYENLSKLVGKEILVAVRSSATAEDIESASFAGQQETY
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCE
LNVSKGELLDAVKKVWASLYTARAISYRRFKGIDQITVEMAVVVQKMVNSRSAGVMFTLH
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEE
PVTGDRNYIMIESSWGLGEAVVSGKVTPDEVVIEKSSLKIVEKKVSHKILKIVYDREKRQ
ECCCCCCEEEEECCCCCCHHHHCCCCCCHHEEEEHHHHHHHHHHHHHHHHHHHHCCCCCC
NIIINLSNEEADQMSISDEEAIELARLALKIEEHYGRPMDIEWAIDADMKFPDNVFIVQA
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEE
RPETYWTSKQIERKKEEVKVSVGKVLTKGLPASPGIAYGKARVILDVKEAKDFEKGDILV
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEHHCCCCCCCCEEE
TKMTDPDWVPIMKIASAIVTDEGGMTSHAAIVSRELGIPAVVGTGNATKVIKDGEEITVD
EECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEECCCCCEEEECCCEEEHH
AIRGIIYEGKLTFETEEKKEATQPVSVGGISRDVLLSLYPVTATKIYMNLGQPDIIHKYL
HHHCEEEECEEEEECHHHHHHCCCCCCCCCCHHHHHEEECCEEEEEEEECCCHHHHHHHH
DLPFDGIGLMRIEFIVSEWIKYHPLYLIKTGKPELFVDKLAEGISMVASAIYPRPVVVRF
CCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
SDFKTNEYKRLIGGEEFEPEERNPMIGWRGVSRYVSPQYEPAFRLEVRAIRKVREEMGLK
ECCCCHHHHHHHCCCCCCCCCCCCCEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
NVWVMFPFVRTTWELEKAIKIMEEEGVRRGQDFKVWIMAEVPSVVVLADKFSQLVDGFSI
EEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEHHHHHHHHCCCCC
GSNDLAQLTLGVDRDSDLLARMGYYDERDPAVLESIKRLIKVAHKYGRTVSICGQAPSVY
CCCCCEEEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
PEINEFLVRQGIDSISVNPDAVIQVKRQVASIEQKIMLEKLRKK
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7805870 [H]