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The map label for this gene is trpB1

Identifier: 15921496

GI number: 15921496

Start: 1233823

End: 1235091

Strand: Reverse

Name: trpB1

Synonym: ST1233

Alternate gene names: 15921496

Gene position: 1235091-1233823 (Counterclockwise)

Preceding gene: 15921497

Following gene: 15921495

Centisome position: 45.83

GC content: 36.01

Gene sequence:

>1269_bases
ATGGTAAATAAGGATTTGATACCAAAATATTGGTACAATATAATACCAGATCTACCTAAACCTTTACCTCCGCCAAGAGA
TCCACCAGATGCTGAATTCTCAAGAATAGAATTATTAAAAAAGATATTACCAAAAGAAGTTTTAAGGCAGCAGTTTACTA
TTGAGCGTTTCATTAAAATACCAGAAGAAGTTAGAGATAGGTACGCTATTATAGGTAGACCAACACCACTCATGAGAGCT
AAAAGATTAGAAGAATACTTAGATACTCCTGCAAAAATATACTTTAAATTTGAAGGCGCTACACCTACAGGCTCACATAA
GATAAATACAGCTGTACCACAAGCATATTTCGCAGCTGAAGAAGGAATTTCTCATGTAGTTACTGAGACTGGTGCAGGGC
AATGGGGTACTGCTGTAGCATTAGCCGCTTCTATGTATGATCTTTCTTCCACAATATTTATGGTAAGAGTAAGTTATGAG
CAGAAACCTATGAGAAAGACCATTATGGAGTTATATGGTGGAAAAGTATATGCTAGCCCTACAGATCTTACTGAATTTGG
AAGAAAAATACTAAAAGAGAATCCTAATCATCCGGGTTCACTAGGAATTGCTATGAGTGAGGCTATTGAATTTGCTTTAA
ACCATAATTTTAGGTATTTAGTGGGTAGTGTTCTTGATGTAGTCCTTCTTCATCAGTCAGTTATAGGGATGGAAGCTATA
GCTCAGCTAGATGAGCTTGGTGAAGAACCAGATATATTAATTGGCTGTGTAGGTGGAGGAAGCAATTTCGGAGGTTTTAC
TTATCCATTTATAGGAGCTAAAAAAGGTAAGAAATATATAGCAGTAGGTGCTGCTGAAATTCCTAAGTTTAGTACAGGTA
AATATGAATATGATTACCCAGATACTGCTGGTCTATTACCTCTTGTAAAGATGATAACTTTAGGAAAAGATTATGTACCT
CCACCGATATATGCTGGGGGATTACGATATCATGGAGTAGCCCCCACACTAAGTTTGCTAATAAAAGAAAAGATAGTTGA
ATGGCGTGAGTATAAAGAGGAAGAGATTTATGAAGCTGCTAAAATATTTATGAAGACACAAGGTATTGTTCCTGCACCTG
AGTCTGCCCATGCAATTAAGGCTGTAATAGATGAGGCATTAAAAGCTAAGACTGAAAAAGAAAGGAGAGTTATAGTATTT
AATTTAAGTGGTCATGGATTACTAGATCTTGGAAATTATGAATCTATCAGAAGAAGGGTAGAAAAATGA

Upstream 100 bases:

>100_bases
TTATGAGTATAAAGGAGAATTTAATAATTTTGATACCTATTAACATTAAGATTTATAAGTGAGATGAGTTTTTAGTCTAT
TAGGTGTTTTTGTTGCACCA

Downstream 100 bases:

>100_bases
GAAGAATGTTAGTAACTTATATGACTTTAGGATACCCTAACTTAGAATCTTTTTATCAATTTATAGAAAAAAGTGTTGAA
TTAGGGACAGATATTTTAGA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MVNKDLIPKYWYNIIPDLPKPLPPPRDPPDAEFSRIELLKKILPKEVLRQQFTIERFIKIPEEVRDRYAIIGRPTPLMRA
KRLEEYLDTPAKIYFKFEGATPTGSHKINTAVPQAYFAAEEGISHVVTETGAGQWGTAVALAASMYDLSSTIFMVRVSYE
QKPMRKTIMELYGGKVYASPTDLTEFGRKILKENPNHPGSLGIAMSEAIEFALNHNFRYLVGSVLDVVLLHQSVIGMEAI
AQLDELGEEPDILIGCVGGGSNFGGFTYPFIGAKKGKKYIAVGAAEIPKFSTGKYEYDYPDTAGLLPLVKMITLGKDYVP
PPIYAGGLRYHGVAPTLSLLIKEKIVEWREYKEEEIYEAAKIFMKTQGIVPAPESAHAIKAVIDEALKAKTEKERRVIVF
NLSGHGLLDLGNYESIRRRVEK

Sequences:

>Translated_422_residues
MVNKDLIPKYWYNIIPDLPKPLPPPRDPPDAEFSRIELLKKILPKEVLRQQFTIERFIKIPEEVRDRYAIIGRPTPLMRA
KRLEEYLDTPAKIYFKFEGATPTGSHKINTAVPQAYFAAEEGISHVVTETGAGQWGTAVALAASMYDLSSTIFMVRVSYE
QKPMRKTIMELYGGKVYASPTDLTEFGRKILKENPNHPGSLGIAMSEAIEFALNHNFRYLVGSVLDVVLLHQSVIGMEAI
AQLDELGEEPDILIGCVGGGSNFGGFTYPFIGAKKGKKYIAVGAAEIPKFSTGKYEYDYPDTAGLLPLVKMITLGKDYVP
PPIYAGGLRYHGVAPTLSLLIKEKIVEWREYKEEEIYEAAKIFMKTQGIVPAPESAHAIKAVIDEALKAKTEKERRVIVF
NLSGHGLLDLGNYESIRRRVEK
>Mature_422_residues
MVNKDLIPKYWYNIIPDLPKPLPPPRDPPDAEFSRIELLKKILPKEVLRQQFTIERFIKIPEEVRDRYAIIGRPTPLMRA
KRLEEYLDTPAKIYFKFEGATPTGSHKINTAVPQAYFAAEEGISHVVTETGAGQWGTAVALAASMYDLSSTIFMVRVSYE
QKPMRKTIMELYGGKVYASPTDLTEFGRKILKENPNHPGSLGIAMSEAIEFALNHNFRYLVGSVLDVVLLHQSVIGMEAI
AQLDELGEEPDILIGCVGGGSNFGGFTYPFIGAKKGKKYIAVGAAEIPKFSTGKYEYDYPDTAGLLPLVKMITLGKDYVP
PPIYAGGLRYHGVAPTLSLLIKEKIVEWREYKEEEIYEAAKIFMKTQGIVPAPESAHAIKAVIDEALKAKTEKERRVIVF
NLSGHGLLDLGNYESIRRRVEK

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=358, Percent_Identity=29.0502793296089, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321412, Length=373, Percent_Identity=30.8310991957105, Blast_Score=137, Evalue=4e-33,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB1_SULTO (Q971Z5)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377165.1
- ProteinModelPortal:   Q971Z5
- GeneID:   1459231
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1233
- NMPDR:   fig|273063.1.peg.1333
- HOGENOM:   HBG303148
- OMA:   YTLGHDF
- ProtClustDB:   PRK12391
- BioCyc:   STOK273063:ST1233-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026
- TIGRFAMs:   TIGR01415

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 47166; Mature: 47166

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNKDLIPKYWYNIIPDLPKPLPPPRDPPDAEFSRIELLKKILPKEVLRQQFTIERFIKI
CCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PEEVRDRYAIIGRPTPLMRAKRLEEYLDTPAKIYFKFEGATPTGSHKINTAVPQAYFAAE
CHHHHHCEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHCCHHHHHHH
EGISHVVTETGAGQWGTAVALAASMYDLSSTIFMVRVSYEQKPMRKTIMELYGGKVYASP
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHCCEEEECC
TDLTEFGRKILKENPNHPGSLGIAMSEAIEFALNHNFRYLVGSVLDVVLLHQSVIGMEAI
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
AQLDELGEEPDILIGCVGGGSNFGGFTYPFIGAKKGKKYIAVGAAEIPKFSTGKYEYDYP
HHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCCCEECCCC
DTAGLLPLVKMITLGKDYVPPPIYAGGLRYHGVAPTLSLLIKEKIVEWREYKEEEIYEAA
CCCHHHHHHHHHHHCCCCCCCCCEECCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIFMKTQGIVPAPESAHAIKAVIDEALKAKTEKERRVIVFNLSGHGLLDLGNYESIRRRV
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEECCCHHHHHHHH
EK
CC
>Mature Secondary Structure
MVNKDLIPKYWYNIIPDLPKPLPPPRDPPDAEFSRIELLKKILPKEVLRQQFTIERFIKI
CCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PEEVRDRYAIIGRPTPLMRAKRLEEYLDTPAKIYFKFEGATPTGSHKINTAVPQAYFAAE
CHHHHHCEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHCCHHHHHHH
EGISHVVTETGAGQWGTAVALAASMYDLSSTIFMVRVSYEQKPMRKTIMELYGGKVYASP
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHCCEEEECC
TDLTEFGRKILKENPNHPGSLGIAMSEAIEFALNHNFRYLVGSVLDVVLLHQSVIGMEAI
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
AQLDELGEEPDILIGCVGGGSNFGGFTYPFIGAKKGKKYIAVGAAEIPKFSTGKYEYDYP
HHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCCCEECCCC
DTAGLLPLVKMITLGKDYVPPPIYAGGLRYHGVAPTLSLLIKEKIVEWREYKEEEIYEAA
CCCHHHHHHHHHHHCCCCCCCCCEECCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIFMKTQGIVPAPESAHAIKAVIDEALKAKTEKERRVIVFNLSGHGLLDLGNYESIRRRV
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEECCCHHHHHHHH
EK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479