Click here to switch to the map view.

The map label for this gene is trpA

Identifier: 15921495

GI number: 15921495

Start: 1233089

End: 1233826

Strand: Reverse

Name: trpA

Synonym: ST1232

Alternate gene names: 15921495

Gene position: 1233826-1233089 (Counterclockwise)

Preceding gene: 15921496

Following gene: 15921494

Centisome position: 45.79

GC content: 29.95

Gene sequence:

>738_bases
ATGAGAAGAATGTTAGTAACTTATATGACTTTAGGATACCCTAACTTAGAATCTTTTTATCAATTTATAGAAAAAAGTGT
TGAATTAGGGACAGATATTTTAGAGATTGGTTTGCCACCTAAATATGCTAAATATGACGGTCCAGTTATAAGAAAAAGTT
ATAAAGCCGTTACAAGTTGGTTAAAAGATTATATAACTCCTTTAAAAGAGGTAAGGAAAAAAGTAAATATTCCAATTATT
ATACTTACGTATCTCGAAGATTATCTTTCTAATTTAGATAACTTCTTAACTACTCTTCATGAAATAGGAATAGACGGGGT
TTTATTTCCAGATTTATTAATAGATTTCATAGATGAGTATGAGGAGTATGTTAGTAAAATAAAGGGAAAAGGTGTAAAAG
CTGTCTTATTTACTGGTCCTTCATTACCTGATAATTTAATTATAAAAGCTTCTAGAATTTCAGATATTTTCCTTTATTAT
GGTGTTAGACCTACAACAGGTATAATTATTCCAGTTAGCGTGGATTCATTAATTACAAGAGTAAGGAATTTGGTTCAAAA
TAAACTAGTTGTGGGCTTTGGTTTGAATGACTTTAATGACTTAAGAAAAGCCTTAAGCGCTGGAGCTGATGGTGTAGCCA
TAGGTACAGCTTTCATAGAAGAAATAGAAAAAAATGGTATTCAATCAGCTTTACATTTAGTCAAAACTATTAGAGGGATA
TTAGATGAATACAGCTGA

Upstream 100 bases:

>100_bases
CTAAGACTGAAAAAGAAAGGAGAGTTATAGTATTTAATTTAAGTGGTCATGGATTACTAGATCTTGGAAATTATGAATCT
ATCAGAAGAAGGGTAGAAAA

Downstream 100 bases:

>100_bases
ATTACTAAGAAAAATAATCAGAAGAGAAAATCTAACTGAAGATGAAGCAAGAAGTATAGCTAATAGCGTAATGAAGGCAG
AAGTACCAGAAATAGTTACT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MRRMLVTYMTLGYPNLESFYQFIEKSVELGTDILEIGLPPKYAKYDGPVIRKSYKAVTSWLKDYITPLKEVRKKVNIPII
ILTYLEDYLSNLDNFLTTLHEIGIDGVLFPDLLIDFIDEYEEYVSKIKGKGVKAVLFTGPSLPDNLIIKASRISDIFLYY
GVRPTTGIIIPVSVDSLITRVRNLVQNKLVVGFGLNDFNDLRKALSAGADGVAIGTAFIEEIEKNGIQSALHLVKTIRGI
LDEYS

Sequences:

>Translated_245_residues
MRRMLVTYMTLGYPNLESFYQFIEKSVELGTDILEIGLPPKYAKYDGPVIRKSYKAVTSWLKDYITPLKEVRKKVNIPII
ILTYLEDYLSNLDNFLTTLHEIGIDGVLFPDLLIDFIDEYEEYVSKIKGKGVKAVLFTGPSLPDNLIIKASRISDIFLYY
GVRPTTGIIIPVSVDSLITRVRNLVQNKLVVGFGLNDFNDLRKALSAGADGVAIGTAFIEEIEKNGIQSALHLVKTIRGI
LDEYS
>Mature_245_residues
MRRMLVTYMTLGYPNLESFYQFIEKSVELGTDILEIGLPPKYAKYDGPVIRKSYKAVTSWLKDYITPLKEVRKKVNIPII
ILTYLEDYLSNLDNFLTTLHEIGIDGVLFPDLLIDFIDEYEEYVSKIKGKGVKAVLFTGPSLPDNLIIKASRISDIFLYY
GVRPTTGIIIPVSVDSLITRVRNLVQNKLVVGFGLNDFNDLRKALSAGADGVAIGTAFIEEIEKNGIQSALHLVKTIRGI
LDEYS

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Saccharomyces cerevisiae, GI6321412, Length=229, Percent_Identity=29.6943231441048, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_SULTO (Q971Z6)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377164.1
- ProteinModelPortal:   Q971Z6
- SMR:   Q971Z6
- GeneID:   1459230
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1232
- NMPDR:   fig|273063.1.peg.1332
- HOGENOM:   HBG508952
- OMA:   NPKYDGP
- ProtClustDB:   PRK13125
- BioCyc:   STOK273063:ST1232-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 27560; Mature: 27560

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 35-35 ACT_SITE 46-46

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRMLVTYMTLGYPNLESFYQFIEKSVELGTDILEIGLPPKYAKYDGPVIRKSYKAVTSW
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
LKDYITPLKEVRKKVNIPIIILTYLEDYLSNLDNFLTTLHEIGIDGVLFPDLLIDFIDEY
HHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EEYVSKIKGKGVKAVLFTGPSLPDNLIIKASRISDIFLYYGVRPTTGIIIPVSVDSLITR
HHHHHHHCCCCCEEEEEECCCCCCHHEEEHHHHHHHHHEECCCCCCCEEEEECHHHHHHH
VRNLVQNKLVVGFGLNDFNDLRKALSAGADGVAIGTAFIEEIEKNGIQSALHLVKTIRGI
HHHHHHCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDEYS
HHCCC
>Mature Secondary Structure
MRRMLVTYMTLGYPNLESFYQFIEKSVELGTDILEIGLPPKYAKYDGPVIRKSYKAVTSW
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
LKDYITPLKEVRKKVNIPIIILTYLEDYLSNLDNFLTTLHEIGIDGVLFPDLLIDFIDEY
HHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EEYVSKIKGKGVKAVLFTGPSLPDNLIIKASRISDIFLYYGVRPTTGIIIPVSVDSLITR
HHHHHHHCCCCCEEEEEECCCCCCHHEEEHHHHHHHHHEECCCCCCCEEEEECHHHHHHH
VRNLVQNKLVVGFGLNDFNDLRKALSAGADGVAIGTAFIEEIEKNGIQSALHLVKTIRGI
HHHHHHCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDEYS
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479