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The map label for this gene is topA [H]

Identifier: 15921479

GI number: 15921479

Start: 1217242

End: 1219236

Strand: Reverse

Name: topA [H]

Synonym: ST1216

Alternate gene names: 15921479

Gene position: 1219236-1217242 (Counterclockwise)

Preceding gene: 15921480

Following gene: 15921474

Centisome position: 45.24

GC content: 30.38

Gene sequence:

>1995_bases
ATGAATTGTTATCCTAAAAATTATATCTTAGTTATTGCAGAAAAAAGTAAAGCTGCAAAGAAAATAGCTGAGGCTTTATC
AGATAAACCAACTACATGTAAAAAATACTCAGTAAGCTATTGGGTTGTAAATTATAAAGGAAAAGTAAACGTTGTTGCAC
CTGCTGCTGGTCATTTATTTAATCTTTATGGTGATAGTTCTTTTCCTATTTTTTCAATGGATTGGAAACCTCTTTGGGAA
ATAGATGATTCTGCTAAATATACAAAAAAGTACTATAATTTGCTTAAAGATTTATCAAAATATGCCATTGAATATATTAA
TGCTTGTGATTATGATATTGAAGGTTCTGTAATAGGTTATATGATAATCAAACATTTTGGTGATTTAAAAAAAGCTAAAA
GAATGAAATTTTCAGCGTTAACTAAACAAGACATACAGAAAGCATATTCAAATCTTTCACCATTAGACTACAATATGATA
AATGCAGGTATTGCTAGGCATAAAGTAGATTGGATATGGGGTATTAACGTAAGTAGGGCCTTAATGTTAGCTGTAAGTTC
TGTTACAAAGAAAAGGGTGACATTAAGTGCGGGGAGGGTACAAAGTCCTACTCTAATTCATGTAGTAGAAAATGAGATAG
CAAGAGAACTATATATTCCATTGCCTGTATATAGAGTTAGAGTAAAAATTAAAATAGGAAATGAGACATTTAGCTTGTCG
TTAAATAGAACGTTCGAATACAAGGAAGAAGCTCAAGAGTTTGCTAATAAAATAAAAAAATCAAGGGCTGTAGTGGATGA
GGTCAGAATAGAGGAAAAACCATTAACACGACCTTCACCTTTTAATCTAGGAGATTTACAAGCAGAAGCTGGTAGAATAT
TAGGCTATTCACCTTATTATGTCGAAAGATTAGCTGAAGAATTATACTTAGATGGGTTAATTAGCTATCCTAGAACAAAT
AGCCAAAAAATTCCCCCATCAGTTGATATTAAAAGTATAGTTAATGGATTAGAAGCTAATTTTAAGAACTTGGTAGGGTT
AGTGAGAAACATTACGAAAGGAGAATACATAGTCAGACAAGGAAGTAAAGATGATCCCGCTCATCCTGCAATTTACCCTA
CTGGAAATAGAATAACTGAAAAACTTGGGATTAAACAATATAAGTTGTTTGAACTAATAACTAGGCGTTTTCTTGCAAGC
ATTTCAAGAGATGCTATAATGATAACTCAGAAAATAATCATACGGTTTAAGGATGTGGATATATCTGATGAGATAACTAG
GCAAAAAATAAAGTTTAAAGGATGGTTATTACTATACCCATATCATTCTGTGAAAGATGAGGAATTATTAGATGTAAAAG
AAGGAGAAGAGGTTAAGATAGAATCTGTTTCTGTAAAGATGGATTTGGCTAAACCGGATGTAAAAAGATACAACAAAGTT
CAATTATTAAAATGGATGGAAACTTCTAACTTAGGAACTGAGGCTACAAGAGGAAAAATTATTGAAACCTTATTTAATAG
AAAATATCTTTCACAAAAAAGAGGTACAATATATCCTACATCGCTAGGAATTGTTATAGTTGAGGTGTTGAAAGATTATT
TTAAGGACTTAACAAGCATAGAATTAACTTCAAAGATGGAAGAAAAATTAAACGAAATACTAGATGGGAAAGAGACAGTA
GAAGGAGTTGTCGCAGAAACAATAAATACTCTGAGGATCTATCTAGATAATTATAATAAATATAAAGATCAAATAGGAAT
TAAATTAGCTAAAATATTAGGATTAATGAAGTATAAGAAATGTAAGTTCTGTGACCTTGAGGCAGAATATGATAATTTAT
GTAAATATCACTATAATGCATATATGAAATTGAAAGAAACACTTAAGGTATGGAAAGAGAGAACTAAATTAGAAGATAAA
GATATATTGAAAAAAATTTATAGTTCGAAAACTACGGGAAAATATATAAAGGACATGATTTCAGAGATGATGTGA

Upstream 100 bases:

>100_bases
GAAGAGCTTAAGCGTGGGGTCTTACCTATTACAGTAAGGCGTGTATATCCTAATGGGAAGATAGAGTTGGTTTCAGTTAA
AAAGGTAGAGTTTAAATAGT

Downstream 100 bases:

>100_bases
GTCATGCCAGCTTGATTTGATGATATGAACCCTGGGGTATCTGATCTAAAGTGCTTTAAACTTTTCATGCCATGCTTCAT
ATGCCTTTATCATCTCTTGG

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 664; Mature: 664

Protein sequence:

>664_residues
MNCYPKNYILVIAEKSKAAKKIAEALSDKPTTCKKYSVSYWVVNYKGKVNVVAPAAGHLFNLYGDSSFPIFSMDWKPLWE
IDDSAKYTKKYYNLLKDLSKYAIEYINACDYDIEGSVIGYMIIKHFGDLKKAKRMKFSALTKQDIQKAYSNLSPLDYNMI
NAGIARHKVDWIWGINVSRALMLAVSSVTKKRVTLSAGRVQSPTLIHVVENEIARELYIPLPVYRVRVKIKIGNETFSLS
LNRTFEYKEEAQEFANKIKKSRAVVDEVRIEEKPLTRPSPFNLGDLQAEAGRILGYSPYYVERLAEELYLDGLISYPRTN
SQKIPPSVDIKSIVNGLEANFKNLVGLVRNITKGEYIVRQGSKDDPAHPAIYPTGNRITEKLGIKQYKLFELITRRFLAS
ISRDAIMITQKIIIRFKDVDISDEITRQKIKFKGWLLLYPYHSVKDEELLDVKEGEEVKIESVSVKMDLAKPDVKRYNKV
QLLKWMETSNLGTEATRGKIIETLFNRKYLSQKRGTIYPTSLGIVIVEVLKDYFKDLTSIELTSKMEEKLNEILDGKETV
EGVVAETINTLRIYLDNYNKYKDQIGIKLAKILGLMKYKKCKFCDLEAEYDNLCKYHYNAYMKLKETLKVWKERTKLEDK
DILKKIYSSKTTGKYIKDMISEMM

Sequences:

>Translated_664_residues
MNCYPKNYILVIAEKSKAAKKIAEALSDKPTTCKKYSVSYWVVNYKGKVNVVAPAAGHLFNLYGDSSFPIFSMDWKPLWE
IDDSAKYTKKYYNLLKDLSKYAIEYINACDYDIEGSVIGYMIIKHFGDLKKAKRMKFSALTKQDIQKAYSNLSPLDYNMI
NAGIARHKVDWIWGINVSRALMLAVSSVTKKRVTLSAGRVQSPTLIHVVENEIARELYIPLPVYRVRVKIKIGNETFSLS
LNRTFEYKEEAQEFANKIKKSRAVVDEVRIEEKPLTRPSPFNLGDLQAEAGRILGYSPYYVERLAEELYLDGLISYPRTN
SQKIPPSVDIKSIVNGLEANFKNLVGLVRNITKGEYIVRQGSKDDPAHPAIYPTGNRITEKLGIKQYKLFELITRRFLAS
ISRDAIMITQKIIIRFKDVDISDEITRQKIKFKGWLLLYPYHSVKDEELLDVKEGEEVKIESVSVKMDLAKPDVKRYNKV
QLLKWMETSNLGTEATRGKIIETLFNRKYLSQKRGTIYPTSLGIVIVEVLKDYFKDLTSIELTSKMEEKLNEILDGKETV
EGVVAETINTLRIYLDNYNKYKDQIGIKLAKILGLMKYKKCKFCDLEAEYDNLCKYHYNAYMKLKETLKVWKERTKLEDK
DILKKIYSSKTTGKYIKDMISEMM
>Mature_664_residues
MNCYPKNYILVIAEKSKAAKKIAEALSDKPTTCKKYSVSYWVVNYKGKVNVVAPAAGHLFNLYGDSSFPIFSMDWKPLWE
IDDSAKYTKKYYNLLKDLSKYAIEYINACDYDIEGSVIGYMIIKHFGDLKKAKRMKFSALTKQDIQKAYSNLSPLDYNMI
NAGIARHKVDWIWGINVSRALMLAVSSVTKKRVTLSAGRVQSPTLIHVVENEIARELYIPLPVYRVRVKIKIGNETFSLS
LNRTFEYKEEAQEFANKIKKSRAVVDEVRIEEKPLTRPSPFNLGDLQAEAGRILGYSPYYVERLAEELYLDGLISYPRTN
SQKIPPSVDIKSIVNGLEANFKNLVGLVRNITKGEYIVRQGSKDDPAHPAIYPTGNRITEKLGIKQYKLFELITRRFLAS
ISRDAIMITQKIIIRFKDVDISDEITRQKIKFKGWLLLYPYHSVKDEELLDVKEGEEVKIESVSVKMDLAKPDVKRYNKV
QLLKWMETSNLGTEATRGKIIETLFNRKYLSQKRGTIYPTSLGIVIVEVLKDYFKDLTSIELTSKMEEKLNEILDGKETV
EGVVAETINTLRIYLDNYNKYKDQIGIKLAKILGLMKYKKCKFCDLEAEYDNLCKYHYNAYMKLKETLKVWKERTKLEDK
DILKKIYSSKTTGKYIKDMISEMM

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic type I/III topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=580, Percent_Identity=26.8965517241379, Blast_Score=143, Evalue=6e-34,
Organism=Homo sapiens, GI4507635, Length=699, Percent_Identity=25.6080114449213, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI1787529, Length=535, Percent_Identity=26.3551401869159, Blast_Score=132, Evalue=1e-31,
Organism=Escherichia coli, GI1788061, Length=635, Percent_Identity=24.8818897637795, Blast_Score=124, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17555378, Length=490, Percent_Identity=26.3265306122449, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI32563869, Length=589, Percent_Identity=24.2784380305603, Blast_Score=108, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323263, Length=658, Percent_Identity=24.9240121580547, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24585251, Length=613, Percent_Identity=24.9592169657423, Blast_Score=138, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24640096, Length=698, Percent_Identity=23.2091690544413, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013826
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005739
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 76603; Mature: 76603

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNCYPKNYILVIAEKSKAAKKIAEALSDKPTTCKKYSVSYWVVNYKGKVNVVAPAAGHLF
CCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCEEEEEECCCCCEE
NLYGDSSFPIFSMDWKPLWEIDDSAKYTKKYYNLLKDLSKYAIEYINACDYDIEGSVIGY
EECCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
MIIKHFGDLKKAKRMKFSALTKQDIQKAYSNLSPLDYNMINAGIARHKVDWIWGINVSRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCEEEEECCCHHHH
LMLAVSSVTKKRVTLSAGRVQSPTLIHVVENEIARELYIPLPVYRVRVKIKIGNETFSLS
HHHHHHHHHHHHEEEECCCCCCCEEEEEHHHHHHHHHCCCCCEEEEEEEEEECCEEEEEE
LNRTFEYKEEAQEFANKIKKSRAVVDEVRIEEKPLTRPSPFNLGDLQAEAGRILGYSPYY
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCEECCCHHH
VERLAEELYLDGLISYPRTNSQKIPPSVDIKSIVNGLEANFKNLVGLVRNITKGEYIVRQ
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
GSKDDPAHPAIYPTGNRITEKLGIKQYKLFELITRRFLASISRDAIMITQKIIIRFKDVD
CCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHEEHHHHEEEECCC
ISDEITRQKIKFKGWLLLYPYHSVKDEELLDVKEGEEVKIESVSVKMDLAKPDVKRYNKV
CCHHHHHHHHEECCEEEEECCCCCCCCHHCCCCCCCEEEEEEEEEEEECCCCCHHHCCHH
QLLKWMETSNLGTEATRGKIIETLFNRKYLSQKRGTIYPTSLGIVIVEVLKDYFKDLTSI
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ELTSKMEEKLNEILDGKETVEGVVAETINTLRIYLDNYNKYKDQIGIKLAKILGLMKYKK
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHCC
CKFCDLEAEYDNLCKYHYNAYMKLKETLKVWKERTKLEDKDILKKIYSSKTTGKYIKDMI
CCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHH
SEMM
HHCC
>Mature Secondary Structure
MNCYPKNYILVIAEKSKAAKKIAEALSDKPTTCKKYSVSYWVVNYKGKVNVVAPAAGHLF
CCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCEEEEEECCCCCEE
NLYGDSSFPIFSMDWKPLWEIDDSAKYTKKYYNLLKDLSKYAIEYINACDYDIEGSVIGY
EECCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
MIIKHFGDLKKAKRMKFSALTKQDIQKAYSNLSPLDYNMINAGIARHKVDWIWGINVSRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCEEEEECCCHHHH
LMLAVSSVTKKRVTLSAGRVQSPTLIHVVENEIARELYIPLPVYRVRVKIKIGNETFSLS
HHHHHHHHHHHHEEEECCCCCCCEEEEEHHHHHHHHHCCCCCEEEEEEEEEECCEEEEEE
LNRTFEYKEEAQEFANKIKKSRAVVDEVRIEEKPLTRPSPFNLGDLQAEAGRILGYSPYY
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCEECCCHHH
VERLAEELYLDGLISYPRTNSQKIPPSVDIKSIVNGLEANFKNLVGLVRNITKGEYIVRQ
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
GSKDDPAHPAIYPTGNRITEKLGIKQYKLFELITRRFLASISRDAIMITQKIIIRFKDVD
CCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHEEHHHHEEEECCC
ISDEITRQKIKFKGWLLLYPYHSVKDEELLDVKEGEEVKIESVSVKMDLAKPDVKRYNKV
CCHHHHHHHHEECCEEEEECCCCCCCCHHCCCCCCCEEEEEEEEEEEECCCCCHHHCCHH
QLLKWMETSNLGTEATRGKIIETLFNRKYLSQKRGTIYPTSLGIVIVEVLKDYFKDLTSI
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ELTSKMEEKLNEILDGKETVEGVVAETINTLRIYLDNYNKYKDQIGIKLAKILGLMKYKK
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHCC
CKFCDLEAEYDNLCKYHYNAYMKLKETLKVWKERTKLEDKDILKKIYSSKTTGKYIKDMI
CCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHH
SEMM
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]