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The map label for this gene is yiaE [C]

Identifier: 15920922

GI number: 15920922

Start: 678064

End: 678957

Strand: Reverse

Name: yiaE [C]

Synonym: ST0694

Alternate gene names: 15920922

Gene position: 678957-678064 (Counterclockwise)

Preceding gene: 15920925

Following gene: 15920921

Centisome position: 25.2

GC content: 33.0

Gene sequence:

>894_bases
ATGATTATTTCTACAGTCAAATTACCCGAGGAATGTAAGAGATTAATCCCAGTAAAAGATGAAGGTATTACAGAAGATGA
TATAAGAAATGCTGAAATTATAATGCTTTGGCCATCTCAAGCTAGAGAAATTTTACCTAAAGCTGAAAAAGTAAAAATAA
TTCAAACTTTTTCAGCAGGAGTCGATGATTTTCCTTTTGAACTATTAAAGAAAGATCAATTACTGTATTCAAATGCGGGG
GCTTATTCTATATCAGTTGCTGAGCACGCTTTTGCACTTATTTTAGCATTAGCTAAGGGTGTTGGAGTGAAGAAAAGAGT
TGAAACGTATCCCTTGAATAATTCTACAATACTAATTCTAGGTGCTGGTGGTATAGGATCTGAAGTAGCAAAAATAGCAA
AGCTAGGTTTTAATATGTATGTCATTGGTGTATCAAGATCATTTAAAGGAACTTATTATGATGAAAAATATTCACTGAAG
GATCTTGAAAAAGTTATAGGAAGGGCAGATGTTATAGTTGATACGCTTCCATTAAATAAAGAGACTAGAGGTATACTAAA
TTACTCTGTTTTATCAAAAGTTAAGGAAAAATGTATAATTGTAAATGTAGGTAGAGCTGAGACTGTCATAGAAGATGATA
TATATAGGATTTTGAAGGAAAAACCTGGAGTAAGGTTTGGTACTGATGTTTTTTGGAGAGTCAATGGAAAGGAGAATTTT
TCTACCTCTAAACTGTGGGAGTTAGAGAATTTCACTGGAACACCTCACATAGCTGGAGCTACGGCAAATGAAAGGGTATT
AAAGAATGCACTTATACAAGCATGCAAAAATGTTTACAGAATACTTAATGAAGGAGAAGGAGAAAATAAAGTGAAAATTG
AGGATTACATCTAG

Upstream 100 bases:

>100_bases
CGAATATATAAAGCGAACTTATGTTCGTTGAATATAATTCTAGTTTATAAATTTTTAAGTTCTATTTCCAAATAAATATG
TTTATGCTAATTTATTAACA

Downstream 100 bases:

>100_bases
CTGGGGAGTTTTATGGAAGAACATAGAAATAAGGCACTAGAGTACTTAAATGCATCTAAGCTTAATTTTGAACATGGTTT
TTATGATATCGCAACGGTAC

Product: D-3-phosphoglycerate dehydrogenase

Products: 3-phosphohydroxypyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MIISTVKLPEECKRLIPVKDEGITEDDIRNAEIIMLWPSQAREILPKAEKVKIIQTFSAGVDDFPFELLKKDQLLYSNAG
AYSISVAEHAFALILALAKGVGVKKRVETYPLNNSTILILGAGGIGSEVAKIAKLGFNMYVIGVSRSFKGTYYDEKYSLK
DLEKVIGRADVIVDTLPLNKETRGILNYSVLSKVKEKCIIVNVGRAETVIEDDIYRILKEKPGVRFGTDVFWRVNGKENF
STSKLWELENFTGTPHIAGATANERVLKNALIQACKNVYRILNEGEGENKVKIEDYI

Sequences:

>Translated_297_residues
MIISTVKLPEECKRLIPVKDEGITEDDIRNAEIIMLWPSQAREILPKAEKVKIIQTFSAGVDDFPFELLKKDQLLYSNAG
AYSISVAEHAFALILALAKGVGVKKRVETYPLNNSTILILGAGGIGSEVAKIAKLGFNMYVIGVSRSFKGTYYDEKYSLK
DLEKVIGRADVIVDTLPLNKETRGILNYSVLSKVKEKCIIVNVGRAETVIEDDIYRILKEKPGVRFGTDVFWRVNGKENF
STSKLWELENFTGTPHIAGATANERVLKNALIQACKNVYRILNEGEGENKVKIEDYI
>Mature_297_residues
MIISTVKLPEECKRLIPVKDEGITEDDIRNAEIIMLWPSQAREILPKAEKVKIIQTFSAGVDDFPFELLKKDQLLYSNAG
AYSISVAEHAFALILALAKGVGVKKRVETYPLNNSTILILGAGGIGSEVAKIAKLGFNMYVIGVSRSFKGTYYDEKYSLK
DLEKVIGRADVIVDTLPLNKETRGILNYSVLSKVKEKCIIVNVGRAETVIEDDIYRILKEKPGVRFGTDVFWRVNGKENF
STSKLWELENFTGTPHIAGATANERVLKNALIQACKNVYRILNEGEGENKVKIEDYI

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082289, Length=240, Percent_Identity=27.9166666666667, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17532191, Length=232, Percent_Identity=28.448275862069, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI28574286, Length=302, Percent_Identity=30.1324503311258, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI45551003, Length=272, Percent_Identity=29.0441176470588, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585514, Length=272, Percent_Identity=29.0441176470588, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28574282, Length=272, Percent_Identity=29.0441176470588, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45552429, Length=272, Percent_Identity=29.0441176470588, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28574284, Length=272, Percent_Identity=29.0441176470588, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.95

Molecular weight: Translated: 33147; Mature: 33147

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIISTVKLPEECKRLIPVKDEGITEDDIRNAEIIMLWPSQAREILPKAEKVKIIQTFSAG
CEEECCCCCHHHHHHCCCCCCCCCHHHCCCCEEEEECCHHHHHHCCCHHHEEEEEHHCCC
VDDFPFELLKKDQLLYSNAGAYSISVAEHAFALILALAKGVGVKKRVETYPLNNSTILIL
CCCCCHHHHHCCHHEEECCCCEEEHHHHHHHHHHHHHHHCCCCCHHHEEECCCCCEEEEE
GAGGIGSEVAKIAKLGFNMYVIGVSRSFKGTYYDEKYSLKDLEKVIGRADVIVDTLPLNK
ECCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCHHEEEEECCCCC
ETRGILNYSVLSKVKEKCIIVNVGRAETVIEDDIYRILKEKPGVRFGTDVFWRVNGKENF
CHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCCCEECCEEEEEECCCCCC
STSKLWELENFTGTPHIAGATANERVLKNALIQACKNVYRILNEGEGENKVKIEDYI
CHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
>Mature Secondary Structure
MIISTVKLPEECKRLIPVKDEGITEDDIRNAEIIMLWPSQAREILPKAEKVKIIQTFSAG
CEEECCCCCHHHHHHCCCCCCCCCHHHCCCCEEEEECCHHHHHHCCCHHHEEEEEHHCCC
VDDFPFELLKKDQLLYSNAGAYSISVAEHAFALILALAKGVGVKKRVETYPLNNSTILIL
CCCCCHHHHHCCHHEEECCCCEEEHHHHHHHHHHHHHHHCCCCCHHHEEECCCCCEEEEE
GAGGIGSEVAKIAKLGFNMYVIGVSRSFKGTYYDEKYSLKDLEKVIGRADVIVDTLPLNK
ECCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCHHEEEEECCCCC
ETRGILNYSVLSKVKEKCIIVNVGRAETVIEDDIYRILKEKPGVRFGTDVFWRVNGKENF
CHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCCCEECCEEEEEECCCCCC
STSKLWELENFTGTPHIAGATANERVLKNALIQACKNVYRILNEGEGENKVKIEDYI
CHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-phosphoglycerate; NAD+

Specific reaction: 3-phosphoglycerate + NAD+ = 3-phosphohydroxypyruvate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]