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The map label for this gene is amt [H]

Identifier: 15920882

GI number: 15920882

Start: 643486

End: 645042

Strand: Direct

Name: amt [H]

Synonym: ST0661

Alternate gene names: 15920882

Gene position: 643486-645042 (Clockwise)

Preceding gene: 15920880

Following gene: 15920886

Centisome position: 23.88

GC content: 37.25

Gene sequence:

>1557_bases
ATGGAAATTCAAAAAACCTTTCCCCAAAATAATCTTATAAAGTTATTAATAATACTAATTAGTTTTTCAATCATTCTATT
ATCATTACACTCATTTGGACAGACATCATCAAATACTACAGCAGAAATACAGAGTTTAAATCAAAGTATTTTGGCTTTAG
AAAATCATACAGCAGATTACCCATCTGCAGCAGTTCCTAGCTGGTTAGATACGGGTAGTAATGCATGGATGCTTACGGCT
GCAACGTTTGTAGGACTTCAAAGTGTACCTGGTGTAGCACTATATTATGCTGGATTATCCAAAAAGAAATATGCTGTAAA
TTCCGCATTAATGGTATTTTATGCTTTTGCTGCAGTTTTAGTTGTTTGGATGATAGCTGGTTATAATGCTGGTTTTGGTC
ATCCTGCATTACTTAGTATTAACGGTTATGGAATTTTAGGTTATCCATTACCAGCATGGTTAGGTCACTATGAAGCTAGT
CAAACCGTATTTGGTCCAACTGGAACACCGGTAGATATTCCAACATCTACTTACATATTCTTCCAATTTGTCTTTGCCGC
AATAACTCCAGTATTATTAGCTGGAGGAGTATTAGAGAGAATGAATTTCAAAGCATGGATGATATTTGTACCCTTCTGGA
GTTTATTAGTATATAGCCCAGTAGCTTATTGGTTATTTGCTGGTGGATGGTTAAATCAACTAGGAGCTGTAGACTTTAGC
GGAGGATATGTTATCCATGTAGATGCCGGAGTCGGTGCGTTAGCTGCTGCATTAGCAATAGGTCCTAGATTAGCATCTGA
AAGAAAATTAGAAGCTCATAGCTTACCATTAGTTCTTGCAGGAGCTGGTTTAATTTGGTTAGGATGGGATGGATTTAACG
GTGGTGATCCCGGTGGTGCAACAATCGATGCTGCAATAGCAGTATTAAATACAAATATTGCAACAGCTGTTAGTGCAATA
ACTTGGATGTTAATGGATATGGCATTCTTTAAGAAACCAACATTAGTGGGTGCAACAAGTGGTGCAATAACTGGACTTGT
AGCTATTACGCCAGCTGCAGGATATGTAAATGGTTGGGAAGCAATGCTTATTGGAATTGCATCTGGAAGTATACCATGGT
TATCTCTGTATAAATTTGAGCCTAGACTGAAAGTTGATGATACATTAGGAGTATTCTCTACTCACGGTATTGCAGGAATA
GTGGGAGGATTGTTAACTGGTGTGTTCGCAGATCCAAATGTGACACAATATGTGGATCCAACGTTAAAAGGTGCTTTATA
TGGTAACTTATATCAACTAGGAATACAAGCTGCCGCTGCAGCAGTAGTATTTGTTTATGATTTTGCAATAACTTTTGGTC
TGTTAAAGTTAATAGGACTATTCATACCATTGCAAGCACCACCTGATACATTAGCTATCGGTGATTATGCAATGCATGGA
GAAGTAGCATATTCTGAACTACTAGCTACTTTACCAGAATCGCAGATAAAGAAAGAAGAAATTAGAGAAGAGGCACAAGA
AGAGAAAGAAGATCCAGATGAGAAAAAATCTAAATAA

Upstream 100 bases:

>100_bases
TAATTCGAAAAGTGTTTCGCCTAAAGGCGTTAAAAAACTAAAAAGTTTTAATGAAAATATCAAAAATGCTTATAACCAAT
TTAGAATTAAATGGTTATCG

Downstream 100 bases:

>100_bases
TTTTTATTAAGATAGAATTCTTTATTTTCTCCTAGTAGAAGACCATTCTTTGATAACTTTTTATAGACTCTCATATTTCC
TCAGCCAATAAATTTCTCTA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MEIQKTFPQNNLIKLLIILISFSIILLSLHSFGQTSSNTTAEIQSLNQSILALENHTADYPSAAVPSWLDTGSNAWMLTA
ATFVGLQSVPGVALYYAGLSKKKYAVNSALMVFYAFAAVLVVWMIAGYNAGFGHPALLSINGYGILGYPLPAWLGHYEAS
QTVFGPTGTPVDIPTSTYIFFQFVFAAITPVLLAGGVLERMNFKAWMIFVPFWSLLVYSPVAYWLFAGGWLNQLGAVDFS
GGYVIHVDAGVGALAAALAIGPRLASERKLEAHSLPLVLAGAGLIWLGWDGFNGGDPGGATIDAAIAVLNTNIATAVSAI
TWMLMDMAFFKKPTLVGATSGAITGLVAITPAAGYVNGWEAMLIGIASGSIPWLSLYKFEPRLKVDDTLGVFSTHGIAGI
VGGLLTGVFADPNVTQYVDPTLKGALYGNLYQLGIQAAAAAVVFVYDFAITFGLLKLIGLFIPLQAPPDTLAIGDYAMHG
EVAYSELLATLPESQIKKEEIREEAQEEKEDPDEKKSK

Sequences:

>Translated_518_residues
MEIQKTFPQNNLIKLLIILISFSIILLSLHSFGQTSSNTTAEIQSLNQSILALENHTADYPSAAVPSWLDTGSNAWMLTA
ATFVGLQSVPGVALYYAGLSKKKYAVNSALMVFYAFAAVLVVWMIAGYNAGFGHPALLSINGYGILGYPLPAWLGHYEAS
QTVFGPTGTPVDIPTSTYIFFQFVFAAITPVLLAGGVLERMNFKAWMIFVPFWSLLVYSPVAYWLFAGGWLNQLGAVDFS
GGYVIHVDAGVGALAAALAIGPRLASERKLEAHSLPLVLAGAGLIWLGWDGFNGGDPGGATIDAAIAVLNTNIATAVSAI
TWMLMDMAFFKKPTLVGATSGAITGLVAITPAAGYVNGWEAMLIGIASGSIPWLSLYKFEPRLKVDDTLGVFSTHGIAGI
VGGLLTGVFADPNVTQYVDPTLKGALYGNLYQLGIQAAAAAVVFVYDFAITFGLLKLIGLFIPLQAPPDTLAIGDYAMHG
EVAYSELLATLPESQIKKEEIREEAQEEKEDPDEKKSK
>Mature_518_residues
MEIQKTFPQNNLIKLLIILISFSIILLSLHSFGQTSSNTTAEIQSLNQSILALENHTADYPSAAVPSWLDTGSNAWMLTA
ATFVGLQSVPGVALYYAGLSKKKYAVNSALMVFYAFAAVLVVWMIAGYNAGFGHPALLSINGYGILGYPLPAWLGHYEAS
QTVFGPTGTPVDIPTSTYIFFQFVFAAITPVLLAGGVLERMNFKAWMIFVPFWSLLVYSPVAYWLFAGGWLNQLGAVDFS
GGYVIHVDAGVGALAAALAIGPRLASERKLEAHSLPLVLAGAGLIWLGWDGFNGGDPGGATIDAAIAVLNTNIATAVSAI
TWMLMDMAFFKKPTLVGATSGAITGLVAITPAAGYVNGWEAMLIGIASGSIPWLSLYKFEPRLKVDDTLGVFSTHGIAGI
VGGLLTGVFADPNVTQYVDPTLKGALYGNLYQLGIQAAAAAVVFVYDFAITFGLLKLIGLFIPLQAPPDTLAIGDYAMHG
EVAYSELLATLPESQIKKEEIREEAQEEKEDPDEKKSK

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=426, Percent_Identity=34.5070422535211, Blast_Score=224, Evalue=9e-60,
Organism=Caenorhabditis elegans, GI17550182, Length=339, Percent_Identity=28.9085545722714, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17550180, Length=482, Percent_Identity=24.0663900414938, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI133931100, Length=460, Percent_Identity=25.6521739130435, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI32564042, Length=312, Percent_Identity=25.9615384615385, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324187, Length=461, Percent_Identity=33.1887201735358, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6325396, Length=458, Percent_Identity=28.1659388646288, Blast_Score=157, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6321559, Length=457, Percent_Identity=27.1334792122538, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI161078314, Length=345, Percent_Identity=30.7246376811594, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 55179; Mature: 55179

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIQKTFPQNNLIKLLIILISFSIILLSLHSFGQTSSNTTAEIQSLNQSILALENHTADY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
PSAAVPSWLDTGSNAWMLTAATFVGLQSVPGVALYYAGLSKKKYAVNSALMVFYAFAAVL
CCCCCCHHHCCCCCCEEEHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
VVWMIAGYNAGFGHPALLSINGYGILGYPLPAWLGHYEASQTVFGPTGTPVDIPTSTYIF
HHHHHHCCCCCCCCCEEEEECCCEEECCCHHHHHCCCCCCCEEECCCCCCCCCCCHHHHH
FQFVFAAITPVLLAGGVLERMNFKAWMIFVPFWSLLVYSPVAYWLFAGGWLNQLGAVDFS
HHHHHHHHHHHHHHCHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEEEC
GGYVIHVDAGVGALAAALAIGPRLASERKLEAHSLPLVLAGAGLIWLGWDGFNGGDPGGA
CCEEEEEECCHHHHHHHHHHCCHHCCCCCCHHHCCCEEEECCCEEEEEECCCCCCCCCCC
TIDAAIAVLNTNIATAVSAITWMLMDMAFFKKPTLVGATSGAITGLVAITPAAGYVNGWE
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHEEEEEECCCCCCCCCCE
AMLIGIASGSIPWLSLYKFEPRLKVDDTLGVFSTHGIAGIVGGLLTGVFADPNVTQYVDP
EEEEEECCCCCCEEEEEEECCCCEECCCCCHHHHCCHHHHHHHHHHHHCCCCCCHHHCCC
TLKGALYGNLYQLGIQAAAAAVVFVYDFAITFGLLKLIGLFIPLQAPPDTLAIGDYAMHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHCC
EVAYSELLATLPESQIKKEEIREEAQEEKEDPDEKKSK
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure
MEIQKTFPQNNLIKLLIILISFSIILLSLHSFGQTSSNTTAEIQSLNQSILALENHTADY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
PSAAVPSWLDTGSNAWMLTAATFVGLQSVPGVALYYAGLSKKKYAVNSALMVFYAFAAVL
CCCCCCHHHCCCCCCEEEHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
VVWMIAGYNAGFGHPALLSINGYGILGYPLPAWLGHYEASQTVFGPTGTPVDIPTSTYIF
HHHHHHCCCCCCCCCEEEEECCCEEECCCHHHHHCCCCCCCEEECCCCCCCCCCCHHHHH
FQFVFAAITPVLLAGGVLERMNFKAWMIFVPFWSLLVYSPVAYWLFAGGWLNQLGAVDFS
HHHHHHHHHHHHHHCHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEEEC
GGYVIHVDAGVGALAAALAIGPRLASERKLEAHSLPLVLAGAGLIWLGWDGFNGGDPGGA
CCEEEEEECCHHHHHHHHHHCCHHCCCCCCHHHCCCEEEECCCEEEEEECCCCCCCCCCC
TIDAAIAVLNTNIATAVSAITWMLMDMAFFKKPTLVGATSGAITGLVAITPAAGYVNGWE
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHEEEEEECCCCCCCCCCE
AMLIGIASGSIPWLSLYKFEPRLKVDDTLGVFSTHGIAGIVGGLLTGVFADPNVTQYVDP
EEEEEECCCCCCEEEEEEECCCCEECCCCCHHHHCCHHHHHHHHHHHHCCCCCCHHHCCC
TLKGALYGNLYQLGIQAAAAAVVFVYDFAITFGLLKLIGLFIPLQAPPDTLAIGDYAMHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHCC
EVAYSELLATLPESQIKKEEIREEAQEEKEDPDEKKSK
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]