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The map label for this gene is glcD [H]

Identifier: 15920870

GI number: 15920870

Start: 633543

End: 634865

Strand: Reverse

Name: glcD [H]

Synonym: ST0649

Alternate gene names: 15920870

Gene position: 634865-633543 (Counterclockwise)

Preceding gene: 15920871

Following gene: 15920869

Centisome position: 23.56

GC content: 34.32

Gene sequence:

>1323_bases
GTGCTTGAAGGAATTGAATACAGTCAAGGAGAAGAAAGAGAAGATTTCGTAGGATTTAAGATTAAGCCTAAAATAGTTGT
TTATCCTAAAAATGAAGAGGAAATTGTCAAAATAGTCAATTATGCTAGAGAGACTAGAACACCCATTGTAACCTGGGGTG
CAGGGACAAGTCTATCTGGGCATTTAATGTGTGATGGATGTATTCTTCTTGATATGAGCAAATACATGAATAAGATAATA
GAAATAAATGAGATAGACTGGTATGCTCATGTACAACCTGGAGTTAACCTAGAATATCTAAATAAGGAATTGAATAAGAA
AGGCTTTTTCTTTCCCCCAGATCCAGCTAGCTTTTTCCTATGTACTGTAGGAGGAGCAACAGCAAACTCATCAGGCGGGA
TGAGAGGTGTAAAATACGGAACTTTTAGGGATTGGGTTTTAGCATTAAAAGTTGTTTTACCAAACGGAAAAGTCATTAAG
CTTGGTGAACCGTTAAGAAAGAATAGAGGAGGATATGATTTAGTACATCTATTTGTGGGAAGTGAAGGAACCTTAGGAAT
TATAACGGAAATATGGCTAAGGATAACGCCCTTGCCAAGAAAGAAAATATATACAGTTTTAGCTTATATGAAAAGTCTTG
AAGATACAGCAGAGACTATAATTGAGCTAAGGAAGAGGAGAATTTTACCAGAAATTTCTGAATATATCGACATAGATGTT
ATAAGAGCTTTAAATAAGAATTTAAATGCGGGATTGAAAGAAAGTGAAGGGGGTGCCTTTATCATATCTGTTGAAGAGGA
TTATCTTGATGAATTGAAAGATATATTAAAAGAAAGAGAGTATATTATTGCTGAAGGAGAAGAGGCTGAAAAAATCTACT
CTATTAGGGCTCAATCAGCTATAGCACTAAGGGCCGAGAGCAAGTATATGTTTGTTGAAGATATAGTTGTTCCAGTATCT
AAATTAATTGATGCTATAAAGAGATTAAAAGAGATCGAAAGAAAATATAACGTCAGAATGCCTGTAATAGCTCACATAGG
AGATGGAAATCTTCACCCTAACATTATGCTTAGTGATACTTCTTTAGCTGAACAAATCTTTGAAGAAGTAGCAAAAATAG
CTATAGAACTTGGTGGTTCAATATCTGGTGAACATGGGATTGGTTACCAAAAGGCTAAACTACTTGCAGAGCAAATTGTT
AGTCATAATGGTGAAGAAGTACTTAAAATAATGAAGGGGATAAAAGACCTAATAGATCCTTATGATATTATGAACCCTAA
TAAGTATGTTGAATTAGCATATAAATTGTGGGAAAAGAAATGA

Upstream 100 bases:

>100_bases
CAGTATACTCGTTGTTAGACAAGCAACCAAGCGAAGAGGAAATAAAAGAGAGCATCGATAACATCAAAAAAATTATTACA
AAGTATTATAATGATTAATT

Downstream 100 bases:

>100_bases
GAGCTGTATTTCCGGGACGATTTCAGCCATTCCACTTAGGTCATTTAGCTGTAATAGAGTGGTTACTCAGCAAATATGAC
GAACTAATTATAGTTGTAGG

Product: glycolate oxidase subunit GlcD

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MLEGIEYSQGEEREDFVGFKIKPKIVVYPKNEEEIVKIVNYARETRTPIVTWGAGTSLSGHLMCDGCILLDMSKYMNKII
EINEIDWYAHVQPGVNLEYLNKELNKKGFFFPPDPASFFLCTVGGATANSSGGMRGVKYGTFRDWVLALKVVLPNGKVIK
LGEPLRKNRGGYDLVHLFVGSEGTLGIITEIWLRITPLPRKKIYTVLAYMKSLEDTAETIIELRKRRILPEISEYIDIDV
IRALNKNLNAGLKESEGGAFIISVEEDYLDELKDILKEREYIIAEGEEAEKIYSIRAQSAIALRAESKYMFVEDIVVPVS
KLIDAIKRLKEIERKYNVRMPVIAHIGDGNLHPNIMLSDTSLAEQIFEEVAKIAIELGGSISGEHGIGYQKAKLLAEQIV
SHNGEEVLKIMKGIKDLIDPYDIMNPNKYVELAYKLWEKK

Sequences:

>Translated_440_residues
MLEGIEYSQGEEREDFVGFKIKPKIVVYPKNEEEIVKIVNYARETRTPIVTWGAGTSLSGHLMCDGCILLDMSKYMNKII
EINEIDWYAHVQPGVNLEYLNKELNKKGFFFPPDPASFFLCTVGGATANSSGGMRGVKYGTFRDWVLALKVVLPNGKVIK
LGEPLRKNRGGYDLVHLFVGSEGTLGIITEIWLRITPLPRKKIYTVLAYMKSLEDTAETIIELRKRRILPEISEYIDIDV
IRALNKNLNAGLKESEGGAFIISVEEDYLDELKDILKEREYIIAEGEEAEKIYSIRAQSAIALRAESKYMFVEDIVVPVS
KLIDAIKRLKEIERKYNVRMPVIAHIGDGNLHPNIMLSDTSLAEQIFEEVAKIAIELGGSISGEHGIGYQKAKLLAEQIV
SHNGEEVLKIMKGIKDLIDPYDIMNPNKYVELAYKLWEKK
>Mature_440_residues
MLEGIEYSQGEEREDFVGFKIKPKIVVYPKNEEEIVKIVNYARETRTPIVTWGAGTSLSGHLMCDGCILLDMSKYMNKII
EINEIDWYAHVQPGVNLEYLNKELNKKGFFFPPDPASFFLCTVGGATANSSGGMRGVKYGTFRDWVLALKVVLPNGKVIK
LGEPLRKNRGGYDLVHLFVGSEGTLGIITEIWLRITPLPRKKIYTVLAYMKSLEDTAETIIELRKRRILPEISEYIDIDV
IRALNKNLNAGLKESEGGAFIISVEEDYLDELKDILKEREYIIAEGEEAEKIYSIRAQSAIALRAESKYMFVEDIVVPVS
KLIDAIKRLKEIERKYNVRMPVIAHIGDGNLHPNIMLSDTSLAEQIFEEVAKIAIELGGSISGEHGIGYQKAKLLAEQIV
SHNGEEVLKIMKGIKDLIDPYDIMNPNKYVELAYKLWEKK

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=438, Percent_Identity=31.2785388127854, Blast_Score=204, Evalue=9e-53,
Organism=Homo sapiens, GI37595754, Length=463, Percent_Identity=29.8056155507559, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI119964728, Length=425, Percent_Identity=27.0588235294118, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI4501993, Length=470, Percent_Identity=25.9574468085106, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1789351, Length=443, Percent_Identity=28.2167042889391, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1787977, Length=498, Percent_Identity=26.5060240963855, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI48994907, Length=175, Percent_Identity=31.4285714285714, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI48994914, Length=179, Percent_Identity=28.4916201117318, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71992373, Length=427, Percent_Identity=31.3817330210773, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI71992381, Length=426, Percent_Identity=30.0469483568075, Blast_Score=176, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI71992392, Length=367, Percent_Identity=32.1525885558583, Blast_Score=167, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17534361, Length=444, Percent_Identity=28.8288288288288, Blast_Score=161, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17556096, Length=462, Percent_Identity=26.6233766233766, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320027, Length=431, Percent_Identity=28.538283062645, Blast_Score=189, Evalue=9e-49,
Organism=Saccharomyces cerevisiae, GI6320023, Length=442, Percent_Identity=29.6380090497738, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6320764, Length=444, Percent_Identity=27.027027027027, Blast_Score=127, Evalue=3e-30,
Organism=Drosophila melanogaster, GI18921117, Length=451, Percent_Identity=28.6031042128603, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24639277, Length=451, Percent_Identity=28.6031042128603, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24639275, Length=451, Percent_Identity=28.6031042128603, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24653753, Length=224, Percent_Identity=30.8035714285714, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 49748; Mature: 49748

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEGIEYSQGEEREDFVGFKIKPKIVVYPKNEEEIVKIVNYARETRTPIVTWGAGTSLSG
CCCCCCCCCCCCCCCCCCEEECCEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCCC
HLMCDGCILLDMSKYMNKIIEINEIDWYAHVQPGVNLEYLNKELNKKGFFFPPDPASFFL
CEEECCCCEEHHHHHHHHHEEEECCCEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCEEE
CTVGGATANSSGGMRGVKYGTFRDWVLALKVVLPNGKVIKLGEPLRKNRGGYDLVHLFVG
EEECCCCCCCCCCCCCCCCCCHHHHHHHHHEECCCCCEEECCCHHHHCCCCCEEEEEEEC
SEGTLGIITEIWLRITPLPRKKIYTVLAYMKSLEDTAETIIELRKRRILPEISEYIDIDV
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
IRALNKNLNAGLKESEGGAFIISVEEDYLDELKDILKEREYIIAEGEEAEKIYSIRAQSA
HHHHCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCE
IALRAESKYMFVEDIVVPVSKLIDAIKRLKEIERKYNVRMPVIAHIGDGNLHPNIMLSDT
EEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCH
SLAEQIFEEVAKIAIELGGSISGEHGIGYQKAKLLAEQIVSHNGEEVLKIMKGIKDLIDP
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
YDIMNPNKYVELAYKLWEKK
HHCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MLEGIEYSQGEEREDFVGFKIKPKIVVYPKNEEEIVKIVNYARETRTPIVTWGAGTSLSG
CCCCCCCCCCCCCCCCCCEEECCEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCCC
HLMCDGCILLDMSKYMNKIIEINEIDWYAHVQPGVNLEYLNKELNKKGFFFPPDPASFFL
CEEECCCCEEHHHHHHHHHEEEECCCEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCEEE
CTVGGATANSSGGMRGVKYGTFRDWVLALKVVLPNGKVIKLGEPLRKNRGGYDLVHLFVG
EEECCCCCCCCCCCCCCCCCCHHHHHHHHHEECCCCCEEECCCHHHHCCCCCEEEEEEEC
SEGTLGIITEIWLRITPLPRKKIYTVLAYMKSLEDTAETIIELRKRRILPEISEYIDIDV
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
IRALNKNLNAGLKESEGGAFIISVEEDYLDELKDILKEREYIIAEGEEAEKIYSIRAQSA
HHHHCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCE
IALRAESKYMFVEDIVVPVSKLIDAIKRLKEIERKYNVRMPVIAHIGDGNLHPNIMLSDT
EEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCH
SLAEQIFEEVAKIAIELGGSISGEHGIGYQKAKLLAEQIVSHNGEEVLKIMKGIKDLIDP
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
YDIMNPNKYVELAYKLWEKK
HHCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]