Click here to switch to the map view.

The map label for this gene is pycA [H]

Identifier: 15920812

GI number: 15920812

Start: 580461

End: 582002

Strand: Reverse

Name: pycA [H]

Synonym: ST0593

Alternate gene names: 15920812

Gene position: 582002-580461 (Counterclockwise)

Preceding gene: 15920813

Following gene: 15920811

Centisome position: 21.6

GC content: 36.38

Gene sequence:

>1542_bases
ATGCCACCTTTTGGAAAAGTACTCGTTGCAAATAGAGGAGAAATTGCAGTAAGAGTTATGAAAGCAATCAAAGAAATGGG
CATGAAAGCTGTTGCTGTGTATTCAGAAGCTGATAAATATGCTTTGCACGTAAAATATGCAGATGAGGCTTATTATATAG
GGCCGCCACCAGCATTAGAAAGCTACTTAAATATTCAAGCCATTATTGATGCAGCAGAAAAAGCTCATGCTGATGCAGTT
CATCCTGGTTACGGTTTCTTATCTGAAAACGCAGATTTTGCTGAAGCTGTTGTAAAGGCTGGATTAACATGGATTGGACC
ACCAGTAGATGCGATGAGAGCTATTAAGAGTAAGCTAGACGGCAAAAGAATTGCAAAACAAGCTGGAGTACCTATTTCAC
CTGGTTCTGACGGTCCAGTTGATAGTTTAGATGAGGCTCTAAAATTAGCTGAAAAAATTGGCTACCCAATAATGGTAAAA
GCGGCATTCGGTGGTGGAGGTACTGGAATAACTAGGGTTGATAATCCAGACCAGTTAGTCGAAGTTTGGGAAAGGAATAA
AAGATTAGCTTATCAAGCCTTTGGTAAAGCTGATTTATATATTGAAAAGGCGGCAGTTAATCCAAGACATATTGAGTTTC
AGCTAATAGGAGATAAATATGGCAACTATGTAGTAGCATGGGAAAGAGAGTGTACAATTCAAAGAAGAAATCAGAAATTA
ATTGAAGAAGCACCCTCACCAGCTCTAAAAATGGAAGAACGAGAAAGAATGTTTGAACCAATTATCAAGTTCGGTCAAAT
TATTCATTATTACACATTAGGAACATTTGAAACAGTTTTCTCAGATACTACGAGAGAATTCTATTTCCTTGAATTAAATA
AAAGACTTCAAGTTGAACACCCAATTACCGAAATGATATTTAGAATTGACTTAGTAAAACTTCAAATAAATATCGCTGCA
GGTGAACCTTTACCTTTTACCCAAGAAGAATTAAATAAAAGAGTAAGAGGGCACGCAATCGAATATAGAATCAATGCTGA
AGATCCGTTAAATGACTTTACTGGTAGCTCGGGATTTATCACATACTATAAGGAACCGACTGGTCCGGGAGTAAGAGTAG
ATAGTGGTGTTACTTTAGGTAGTTACGTACCACCATTTTACGATTCGTTAATTTCAAAACTCATTGTATATGGCGAAAAT
AGAGCTTATGCCATACAGGCTGGAATAAGAGCTTTAAATGATTATAAAATAGGTGGAGTTAGAACAACAATTGAACTATA
CAAATGGATATCACAAGAGGAAGATTTTCAAAAAGGTAAATTCTCAACTGCATATATAGCTGAGAAGAGAGAACAATACC
TAAAATACTTAAAAGCAAAAGAGCAGATGAAAGCCGCTTTAGCTGCAACGTTATATCAAAGAGGATTGTTAAAGAAAGCA
ACGACTACAGTTAATTCTACTCAACCACAAAATCAAACAAAGAGGTCGAATTGGAAAACATACGGTTTATTGCAACAATC
CTCTTATAGGGTGATGTGGTAA

Upstream 100 bases:

>100_bases
TTTTCTTTTTATAATTATATTCTTTTTATAATCTGTATGATTAAAATTGAAAGATTAAAACTTTTTAAACCATAAATACT
CTATTATAAGTAGGGTAAAA

Downstream 100 bases:

>100_bases
TGAAGTTACTTAGGGTATCTTCAGAATTAGGAGATAGCTATGTAATGACTTACGATCAACAAGGTAATAAAGATACCGTC
AGCTTTGAGGATAATAAGTT

Product: biotin carboxylase

Products: NA

Alternate protein names: Pyruvic carboxylase A [H]

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MPPFGKVLVANRGEIAVRVMKAIKEMGMKAVAVYSEADKYALHVKYADEAYYIGPPPALESYLNIQAIIDAAEKAHADAV
HPGYGFLSENADFAEAVVKAGLTWIGPPVDAMRAIKSKLDGKRIAKQAGVPISPGSDGPVDSLDEALKLAEKIGYPIMVK
AAFGGGGTGITRVDNPDQLVEVWERNKRLAYQAFGKADLYIEKAAVNPRHIEFQLIGDKYGNYVVAWERECTIQRRNQKL
IEEAPSPALKMEERERMFEPIIKFGQIIHYYTLGTFETVFSDTTREFYFLELNKRLQVEHPITEMIFRIDLVKLQINIAA
GEPLPFTQEELNKRVRGHAIEYRINAEDPLNDFTGSSGFITYYKEPTGPGVRVDSGVTLGSYVPPFYDSLISKLIVYGEN
RAYAIQAGIRALNDYKIGGVRTTIELYKWISQEEDFQKGKFSTAYIAEKREQYLKYLKAKEQMKAALAATLYQRGLLKKA
TTTVNSTQPQNQTKRSNWKTYGLLQQSSYRVMW

Sequences:

>Translated_513_residues
MPPFGKVLVANRGEIAVRVMKAIKEMGMKAVAVYSEADKYALHVKYADEAYYIGPPPALESYLNIQAIIDAAEKAHADAV
HPGYGFLSENADFAEAVVKAGLTWIGPPVDAMRAIKSKLDGKRIAKQAGVPISPGSDGPVDSLDEALKLAEKIGYPIMVK
AAFGGGGTGITRVDNPDQLVEVWERNKRLAYQAFGKADLYIEKAAVNPRHIEFQLIGDKYGNYVVAWERECTIQRRNQKL
IEEAPSPALKMEERERMFEPIIKFGQIIHYYTLGTFETVFSDTTREFYFLELNKRLQVEHPITEMIFRIDLVKLQINIAA
GEPLPFTQEELNKRVRGHAIEYRINAEDPLNDFTGSSGFITYYKEPTGPGVRVDSGVTLGSYVPPFYDSLISKLIVYGEN
RAYAIQAGIRALNDYKIGGVRTTIELYKWISQEEDFQKGKFSTAYIAEKREQYLKYLKAKEQMKAALAATLYQRGLLKKA
TTTVNSTQPQNQTKRSNWKTYGLLQQSSYRVMW
>Mature_512_residues
PPFGKVLVANRGEIAVRVMKAIKEMGMKAVAVYSEADKYALHVKYADEAYYIGPPPALESYLNIQAIIDAAEKAHADAVH
PGYGFLSENADFAEAVVKAGLTWIGPPVDAMRAIKSKLDGKRIAKQAGVPISPGSDGPVDSLDEALKLAEKIGYPIMVKA
AFGGGGTGITRVDNPDQLVEVWERNKRLAYQAFGKADLYIEKAAVNPRHIEFQLIGDKYGNYVVAWERECTIQRRNQKLI
EEAPSPALKMEERERMFEPIIKFGQIIHYYTLGTFETVFSDTTREFYFLELNKRLQVEHPITEMIFRIDLVKLQINIAAG
EPLPFTQEELNKRVRGHAIEYRINAEDPLNDFTGSSGFITYYKEPTGPGVRVDSGVTLGSYVPPFYDSLISKLIVYGENR
AYAIQAGIRALNDYKIGGVRTTIELYKWISQEEDFQKGKFSTAYIAEKREQYLKYLKAKEQMKAALAATLYQRGLLKKAT
TTVNSTQPQNQTKRSNWKTYGLLQQSSYRVMW

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain [H]

Homologues:

Organism=Homo sapiens, GI116805327, Length=489, Percent_Identity=42.5357873210634, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI189095269, Length=517, Percent_Identity=41.9729206963249, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI65506442, Length=514, Percent_Identity=42.2178988326848, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI295821183, Length=514, Percent_Identity=42.2178988326848, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI106049528, Length=454, Percent_Identity=41.8502202643172, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI106049295, Length=454, Percent_Identity=41.8502202643172, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI106049292, Length=454, Percent_Identity=41.8502202643172, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI38679971, Length=506, Percent_Identity=31.6205533596838, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI38679960, Length=506, Percent_Identity=31.6205533596838, Blast_Score=236, Evalue=4e-62,
Organism=Homo sapiens, GI38679977, Length=506, Percent_Identity=31.6205533596838, Blast_Score=236, Evalue=4e-62,
Organism=Homo sapiens, GI38679967, Length=506, Percent_Identity=31.6205533596838, Blast_Score=236, Evalue=4e-62,
Organism=Homo sapiens, GI38679974, Length=506, Percent_Identity=31.6205533596838, Blast_Score=236, Evalue=4e-62,
Organism=Homo sapiens, GI134142062, Length=507, Percent_Identity=31.7554240631164, Blast_Score=227, Evalue=2e-59,
Organism=Homo sapiens, GI18105007, Length=356, Percent_Identity=22.752808988764, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1789654, Length=445, Percent_Identity=48.0898876404494, Blast_Score=394, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17567343, Length=505, Percent_Identity=43.7623762376238, Blast_Score=370, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71987519, Length=453, Percent_Identity=42.1633554083885, Blast_Score=357, Evalue=9e-99,
Organism=Caenorhabditis elegans, GI17562816, Length=455, Percent_Identity=42.4175824175824, Blast_Score=343, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI133931226, Length=508, Percent_Identity=29.7244094488189, Blast_Score=204, Evalue=8e-53,
Organism=Caenorhabditis elegans, GI71997168, Length=530, Percent_Identity=27.5471698113208, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI71997163, Length=509, Percent_Identity=27.8978388998035, Blast_Score=182, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6319685, Length=440, Percent_Identity=42.5, Blast_Score=354, Evalue=2e-98,
Organism=Saccharomyces cerevisiae, GI6319695, Length=450, Percent_Identity=39.3333333333333, Blast_Score=330, Evalue=4e-91,
Organism=Saccharomyces cerevisiae, GI6321376, Length=450, Percent_Identity=40.4444444444444, Blast_Score=321, Evalue=2e-88,
Organism=Saccharomyces cerevisiae, GI6323863, Length=472, Percent_Identity=31.7796610169492, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6324343, Length=512, Percent_Identity=29.8828125, Blast_Score=228, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24651757, Length=475, Percent_Identity=40.2105263157895, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI281363050, Length=451, Percent_Identity=43.6807095343681, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24652224, Length=451, Percent_Identity=43.6807095343681, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24652222, Length=451, Percent_Identity=43.6807095343681, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24652220, Length=451, Percent_Identity=43.6807095343681, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24652218, Length=451, Percent_Identity=43.6807095343681, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24652212, Length=456, Percent_Identity=43.2017543859649, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24652210, Length=456, Percent_Identity=43.2017543859649, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24652214, Length=456, Percent_Identity=43.2017543859649, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI19921944, Length=456, Percent_Identity=43.2017543859649, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24652216, Length=456, Percent_Identity=43.2017543859649, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24651759, Length=436, Percent_Identity=39.9082568807339, Blast_Score=329, Evalue=3e-90,
Organism=Drosophila melanogaster, GI161076407, Length=587, Percent_Identity=29.4718909710392, Blast_Score=222, Evalue=5e-58,
Organism=Drosophila melanogaster, GI24586460, Length=587, Percent_Identity=29.4718909710392, Blast_Score=222, Evalue=5e-58,
Organism=Drosophila melanogaster, GI161076409, Length=587, Percent_Identity=29.4718909710392, Blast_Score=222, Evalue=6e-58,
Organism=Drosophila melanogaster, GI24586458, Length=587, Percent_Identity=29.4718909710392, Blast_Score=222, Evalue=6e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004549
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 57765; Mature: 57634

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS50975 ATP_GRASP ; PS00866 CPSASE_1 ; PS50979 BC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPFGKVLVANRGEIAVRVMKAIKEMGMKAVAVYSEADKYALHVKYADEAYYIGPPPALE
CCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCEEECCCCHHHH
SYLNIQAIIDAAEKAHADAVHPGYGFLSENADFAEAVVKAGLTWIGPPVDAMRAIKSKLD
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCC
GKRIAKQAGVPISPGSDGPVDSLDEALKLAEKIGYPIMVKAAFGGGGTGITRVDNPDQLV
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHH
EVWERNKRLAYQAFGKADLYIEKAAVNPRHIEFQLIGDKYGNYVVAWERECTIQRRNQKL
HHHHCCCCEEEHHCCCCEEEEEECCCCCCEEEEEEECCCCCCEEEEEECCHHHHHHHHHH
IEEAPSPALKMEERERMFEPIIKFGQIIHYYTLGTFETVFSDTTREFYFLELNKRLQVEH
HHHCCCCCCCHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCCCEEEEEEECCCEEECC
PITEMIFRIDLVKLQINIAAGEPLPFTQEELNKRVRGHAIEYRINAEDPLNDFTGSSGFI
CHHHHHHHEEEEEEEEEEECCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEE
TYYKEPTGPGVRVDSGVTLGSYVPPFYDSLISKLIVYGENRAYAIQAGIRALNDYKIGGV
EEEECCCCCCEEECCCCEECCCCCHHHHHHHHHHHEECCCCEEEEHHHHHHHCCEECCCC
RTTIELYKWISQEEDFQKGKFSTAYIAEKREQYLKYLKAKEQMKAALAATLYQRGLLKKA
HHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTTVNSTQPQNQTKRSNWKTYGLLQQSSYRVMW
HCCCCCCCCCCHHHHCCCCEEEEEECCCCEECC
>Mature Secondary Structure 
PPFGKVLVANRGEIAVRVMKAIKEMGMKAVAVYSEADKYALHVKYADEAYYIGPPPALE
CCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCEEECCCCHHHH
SYLNIQAIIDAAEKAHADAVHPGYGFLSENADFAEAVVKAGLTWIGPPVDAMRAIKSKLD
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCC
GKRIAKQAGVPISPGSDGPVDSLDEALKLAEKIGYPIMVKAAFGGGGTGITRVDNPDQLV
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHH
EVWERNKRLAYQAFGKADLYIEKAAVNPRHIEFQLIGDKYGNYVVAWERECTIQRRNQKL
HHHHCCCCEEEHHCCCCEEEEEECCCCCCEEEEEEECCCCCCEEEEEECCHHHHHHHHHH
IEEAPSPALKMEERERMFEPIIKFGQIIHYYTLGTFETVFSDTTREFYFLELNKRLQVEH
HHHCCCCCCCHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCCCEEEEEEECCCEEECC
PITEMIFRIDLVKLQINIAAGEPLPFTQEELNKRVRGHAIEYRINAEDPLNDFTGSSGFI
CHHHHHHHEEEEEEEEEEECCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEE
TYYKEPTGPGVRVDSGVTLGSYVPPFYDSLISKLIVYGENRAYAIQAGIRALNDYKIGGV
EEEECCCCCCEEECCCCEECCCCCHHHHHHHHHHHEECCCCEEEEHHHHHHHCCEECCCC
RTTIELYKWISQEEDFQKGKFSTAYIAEKREQYLKYLKAKEQMKAALAATLYQRGLLKKA
HHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTTVNSTQPQNQTKRSNWKTYGLLQQSSYRVMW
HCCCCCCCCCCHHHHCCCCEEEEEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]