Click here to switch to the map view.

The map label for this gene is cobA [H]

Identifier: 15920781

GI number: 15920781

Start: 554062

End: 554781

Strand: Reverse

Name: cobA [H]

Synonym: ST0563

Alternate gene names: 15920781

Gene position: 554781-554062 (Counterclockwise)

Preceding gene: 15920782

Following gene: 15920780

Centisome position: 20.59

GC content: 35.14

Gene sequence:

>720_bases
ATGGGAAAGGTTTTTATTGTAGGTGCAGGTCCAGGAGACCCTGAGCTAATTACACTTAAGGCTTATAACTTGATAAAAAA
GGCTAATGTAATTATTTACGATAGATTAGTTCCAAAGACGTTATTAGAAGATACAAAAGCAGAACTTATATATGTAGGAA
AAGAGATAGGGGACTCAAAACTTCAAGATTATATAAATGAACTCTTAGTAGAAAAAGCAAAGACAAATAGCATTGTTGTG
AGATTAAAGGGGGGAGATCCTTATGTTTTCGGAAGGGGAGAAGAAGAGTGTATTTACGTATTATCTCACGGAATTGAATG
CGAAGTAGTTCCGGGAGTAAGTAGTGCTATTGCAGTGCCGGCTTATGCGGGCATTCCGGTTACAAGCAGATTAACAAACT
CCTCTAGCTTTACAGTAATTACAGGGACTAGGGCTGATAATAAAATCATTGATGCAAGTTATATTCCAAGAGAAGGAACT
CTAATAATCTTGATGGGAATTCACGTAATAGAACAATTAGAGAGAGTTTTACTCTCAGTGAGAGATTATGAGGAATATGT
AGCTATTATAGAGAACGGTACAACTGAAAAACAAAGAGTTATAACGGGAAGGCTAAAAGACTTATCTAAACTAATAATAG
AACATAAAATAAGACCTCCTGCAATAATTGTTGTTGGAGATGTTGTGAAATTAAGAGAGAAACTTTGGAAGTTAACTTAA

Upstream 100 bases:

>100_bases
CCATCATTGTAAATTATATACTACTTCTAGTGTTCGGTCTGAAATTTCCTGAAGGAATATTATTTGGAGTTTATATAATT
AAAAAAGTAAAGGCAATTTG

Downstream 100 bases:

>100_bases
AAACATTATATGAGAACTTTAAAAATATGAAAATTAAAGACCCTGTTTGTGGAATGGATGTAGAAGATTCCACTCCTTAT
AAGTTTACCTATAAGGGAAA

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MGKVFIVGAGPGDPELITLKAYNLIKKANVIIYDRLVPKTLLEDTKAELIYVGKEIGDSKLQDYINELLVEKAKTNSIVV
RLKGGDPYVFGRGEEECIYVLSHGIECEVVPGVSSAIAVPAYAGIPVTSRLTNSSSFTVITGTRADNKIIDASYIPREGT
LIILMGIHVIEQLERVLLSVRDYEEYVAIIENGTTEKQRVITGRLKDLSKLIIEHKIRPPAIIVVGDVVKLREKLWKLT

Sequences:

>Translated_239_residues
MGKVFIVGAGPGDPELITLKAYNLIKKANVIIYDRLVPKTLLEDTKAELIYVGKEIGDSKLQDYINELLVEKAKTNSIVV
RLKGGDPYVFGRGEEECIYVLSHGIECEVVPGVSSAIAVPAYAGIPVTSRLTNSSSFTVITGTRADNKIIDASYIPREGT
LIILMGIHVIEQLERVLLSVRDYEEYVAIIENGTTEKQRVITGRLKDLSKLIIEHKIRPPAIIVVGDVVKLREKLWKLT
>Mature_238_residues
GKVFIVGAGPGDPELITLKAYNLIKKANVIIYDRLVPKTLLEDTKAELIYVGKEIGDSKLQDYINELLVEKAKTNSIVVR
LKGGDPYVFGRGEEECIYVLSHGIECEVVPGVSSAIAVPAYAGIPVTSRLTNSSSFTVITGTRADNKIIDASYIPREGTL
IILMGIHVIEQLERVLLSVRDYEEYVAIIENGTTEKQRVITGRLKDLSKLIIEHKIRPPAIIVVGDVVKLREKLWKLT

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=241, Percent_Identity=40.2489626556017, Blast_Score=155, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6322922, Length=236, Percent_Identity=34.7457627118644, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 26414; Mature: 26283

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVFIVGAGPGDPELITLKAYNLIKKANVIIYDRLVPKTLLEDTKAELIYVGKEIGDSK
CCEEEEEECCCCCCCEEEEEEHHHHHHCCEEEEECCCHHHHHCCCCCEEEEEECCCCHHH
LQDYINELLVEKAKTNSIVVRLKGGDPYVFGRGEEECIYVLSHGIECEVVPGVSSAIAVP
HHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCEEEEEECCCEEEEECCCCCCEECC
AYAGIPVTSRLTNSSSFTVITGTRADNKIIDASYIPREGTLIILMGIHVIEQLERVLLSV
CCCCCCCHHEECCCCCEEEEEECCCCCEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHH
RDYEEYVAIIENGTTEKQRVITGRLKDLSKLIIEHKIRPPAIIVVGDVVKLREKLWKLT
HCHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCC
>Mature Secondary Structure 
GKVFIVGAGPGDPELITLKAYNLIKKANVIIYDRLVPKTLLEDTKAELIYVGKEIGDSK
CEEEEEECCCCCCCEEEEEEHHHHHHCCEEEEECCCHHHHHCCCCCEEEEEECCCCHHH
LQDYINELLVEKAKTNSIVVRLKGGDPYVFGRGEEECIYVLSHGIECEVVPGVSSAIAVP
HHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCEEEEEECCCEEEEECCCCCCEECC
AYAGIPVTSRLTNSSSFTVITGTRADNKIIDASYIPREGTLIILMGIHVIEQLERVLLSV
CCCCCCCHHEECCCCCEEEEEECCCCCEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHH
RDYEEYVAIIENGTTEKQRVITGRLKDLSKLIIEHKIRPPAIIVVGDVVKLREKLWKLT
HCHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]